• Journal of Microbiology and Biotechnology
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• v.22 no.2
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• pp.190-198
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• 2012

RNA interference (RNAi) is rapidly becoming a valuable tool in biological studies, as it allows the selective and transient knockdown of protein expression. The short-interfering RNAs (siRNAs) transiently silence gene expression. By contrast, the expressed short-hairpin RNAs induce long-term, stable knockdown of their target gene. Trichoplusia ni (T. ni) cells are widely used for mammalian cell-derived glycoprotein expression using the baculovirus system. However, a suitable shRNA expression system has not been developed yet. We investigated the potency of shRNA-mediated gene expression inhibition using human and Drosophila U6 promoters in T. ni cells. Luciferase, EGFP, and ${\beta}$-N-acetylglucosaminidase (GlcNAcase) were employed as targets to investigate knockdown of specific genes in T. ni cells. Introduction of the shRNA expression vector under the control of human U6 or Drosophila U6 promoter into T. ni cells exhibited the reduced level of luciferase, EGFP, and ${\beta}$-N-acetylglucosaminidase compared with that of untransfected cells. The shRNA was expressed and processed to siRNA in our vector-transfected T. ni cells. GlcNAcase mRNA levels were down-regulated in T. ni cells transfected with shRNA vectors-targeted GlcNAcase as compared with the control vector-treated cells. It implied that our shRNA expression vectors using human and Drosophila U6 promoters were applied in T. ni cells for the specific gene knockdown.

• Journal of the Korean Institute of Gas
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• v.12 no.2
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• pp.85-91
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• 2008

The deviation of measured pressures in pipeline networks from normal or reference pressures is useful information for judging the operation of the pipeline networks. A cost-effective monitoring of pipeline networks including a leak detection capability can be realized when transient pressure variation is accurately predicted using measured conditions at supply- and demand-sides of the networks. In this study, a pipeline network analysis program was developed based on one-dimensional flow equations for compressible fluids. The validity of the present analysis was demonstrated by simulating the flow in a straight pipeline and comparing the results with the previously reported ones. Pressure and flow rate transients in several simple city-gas pipeline networks were also analyzed to show the usefulness of the developed program.

• Mycobiology
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• v.48 no.5
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• pp.410-417
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• 2020

Fungal ecological interactions play a key role in structuring community assemblages. These associations may involve both antagonistic and synergistic relationships, which are commonly influenced by abiotic factors such as nutrient conditions. However, information for extreme, oligotrophic systems remain poor. Herein, interactions among key members of the aquatic transient fungal community (Aspergillus niger, Cladosporium sp., and Coprinellus micaceus) of a low-nutrient freshwater system in the Cuatro Ci enegas Basin, Mexico were studied. Pairwise interaction bioassays were explored in vitro under different nutrient conditions, including carbohydrates-rich, carbohydrates and amino peptides-rich, and low nutrients. Our results indicated that antagonistic patterns prevail among the studied taxa. However, nutrient-dependent changes were observed in Cladosporium sp. shifting to synergy under carbohydrates-rich conditions, suggesting changes in the fungal community composition as a result of nutrient enrichment. Remarkably, our findings contrast with previous work demonstrating mainly synergistic interactions between our tested fungal isolates and co-occurring autochthonous bacteria (Aeromonas spp. and Vibrio sp.) under low-nutrient conditions. This observation may indicate that bacteria and fungi exhibit distinct community-level responses, driven by nutrient conditions. This contributes to the knowledge of fungal community dynamics and interspecific interactions in an oligotrophic ecosystem, highlighting the relevance of nutrient-based shifts and antagonistic interactions in ecosystem dynamics.

• Nuclear Engineering and Technology
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• v.31 no.5
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• pp.476-485
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• 1999

In order to evaluate the gravity-injection capability to maintain core cooling after a loss-of-shutdown-cooling event during shutdown operation, the plant conditions of the Yong Gwang Units 3&4 were reviewed. The six cases of possible gravity-injection paths from the refueling water tank (RWT) were identified and the thermal-hydraulic analyses were performed using the RELAP5/MOD3.2 code. The core cooling capability was significantly dependent on the gravity-injection path, the RCS opening, and the injection rate. In the cases with the pressurizer manway opening higher than the RWT water level, the coolant was held up in the pressurizer and the system pressure continued increasing after gravity-injection. The gravity injection eventually stopped due to the high system pressure and the core was uncovered. In the cases with the injection path and opening on the same leg side, the core cooling was dependent on whether the water injected from the RWT passed the core region or not. However, in the cases with the injection path and opening on the different leg side, the system was well depressurized after gravity-injection and the core boiling was successfully prevented for a long-term transient. In addition, from the sensitivity study on the gravity-injection flow rate, it was found that about 54 kg/s of injection rate was required to maintain the core cooling and the core cooling could be provided for about 10.6 hours after event with that injection rate from the RWT. Those analysis results would provide useful information to operators coping with the event.

• Journal of Korean Society of Transportation
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• v.21 no.3
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• pp.97-106
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• 2003

When a route choice is done under uncertainty, a driver has some expectation of traffic conditions that will occur according to the route chosen. This study tries to build a framework in which we can observe the learning behavior of the drivers' expectations of the travel time under nonstationary environment. In order to investigate how drivers have their subjective expectations on traffic conditions in response to public information, a numerical experiment is carried out. We found that rational expectations(RE) formation about the route travel time can be expressed by the adaptive expectation model when the travel time changes in accordance with the nonstationary process which consists of permanent shock and transient shock. Also, we found that the adaptive parameter of the model converges to the fixed value corresponding to the route conditions.

• ALGAE
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• v.36 no.4
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• pp.315-326
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• 2021

Ion channels are membrane protein complexes mediating passive ion flux across the cell membranes. Every organism has a certain set of ion channels that define its physiology. Dinoflagellates are ecologically important microorganisms characterized by effective physiological adaptability, which backs up their massive proliferations that often result in harmful blooms (red tides). In this study, we used a bioinformatics approach to identify homologs of known ion channels that belong to 36 ion channel families. We demonstrated that the versatility of the dinoflagellate physiology is underpinned by a high diversity of ion channels including homologs of animal and plant proteins, as well as channels unique to protists. The analysis of 27 transcriptomes allowed reconstructing a consensus ion channel repertoire (channelome) of dinoflagellates including the members of 31 ion channel families: inwardly-rectifying potassium channels, two-pore domain potassium channels, voltage-gated potassium channels (K_v), tandem K_v, cyclic nucleotide-binding domain-containing channels (CNBD), tandem CNBD, eukaryotic ionotropic glutamate receptors, large-conductance calcium-activated potassium channels, intermediate/small-conductance calcium-activated potassium channels, eukaryotic single-domain voltage-gated cation channels, transient receptor potential channels, two-pore domain calcium channels, four-domain voltage-gated cation channels, cation and anion Cys-loop receptors, small-conductivity mechanosensitive channels, large-conductivity mechanosensitive channels, voltage-gated proton channels, inositole-1,4,5-trisphosphate receptors, slow anion channels, aluminum-activated malate transporters and quick anion channels, mitochondrial calcium uniporters, voltage-dependent anion channels, vesicular chloride channels, ionotropic purinergic receptors, animal volage-insensitive cation channels, channelrhodopsins, bestrophins, voltage-gated chloride channels H⁺/Cl^- exchangers, plant calcium-permeable mechanosensitive channels, and trimeric intracellular cation channels. Overall, dinoflagellates represent cells able to respond to physical and chemical stimuli utilizing a wide range of G-protein coupled receptors- and Ca²⁺-dependent signaling pathways. The applied approach not only shed light on the ion channel set in dinoflagellates, but also provided the information on possible molecular mechanisms underlying vital cellular processes dependent on the ion transport.

• Korean Chemical Engineering Research
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• v.57 no.1
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• pp.28-41
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• 2019

We carried out performance analysis of Sodium-Water Reaction Pressure Relief System of Prototype Generation-IV Sodium-Cooled Fast Reactor. We analyzed transient-dynamic behavior of fluids inside the steam generator to vent into a sodium dump tank or a water dump tank when tubes in the steam generator were broken to cause a large-water-leak accident. Accordingly, we preliminarily evaluated design requirements of our system. Our results showed that sodium in the shell side of the steam generator and in Intermediate Heat Transport System was completely vented within 50 s and feed water in the tube side of the steam generator was completely vented within 2.5 s. It was analyzed that pressure of the tube side of the steam generator was higher than pressure of the shell side of the steam generator, which showed that sodium in the shell side did not flow into the tube side. Our results are expected to be used as basis information to performance analysis of Sodium-Water Reaction Pressure Relief System of Prototype Generation-IV Sodium-Cooled Fast Reactor.

• KIPS Transactions on Software and Data Engineering
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• v.8 no.7
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• pp.289-302
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• 2019

Structural change of time series means that the distribution of observations is relatively stable in the period of constituting the entire time series data, but shows a sudden change of the distribution characteristic at a specific time point. Within a non-stationary long-term time series, it is important to determine in a timely manner whether the change in short-term trends is transient or structurally changed. This is because it is necessary to always detect the change of the time series trend and to take appropriate measures to cope with the change. In this paper, we propose a method for decision makers to easily grasp the structural changes of time series by visualizing the test results based on the unit root test. Particularly, it is possible to grasp the short-term structural changes even in the long-term time series through the method of dividing the time series and testing it.

• Nuclear Engineering and Technology
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• v.51 no.6
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• pp.1514-1524
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• 2019

Hydrogen-steam gas mixture may be injected into containment with flow regime varying both spatially and transiently due to wall effect and pressure difference between primary loop and containment in severe accidents induced by loss of coolant accident. Preliminary CFD analysis is conducted to gain information about the helium flow regime transition process from jet to buoyancy plume for forthcoming experimental study. Physical models of impinging jet and wall condensation are validated using separated effect experimental data, firstly. Then helium transportation is analyzed with the effect of jet momentum, buoyancy and wall cooling discussed. Result shows that helium distribution is totally dominated by impinging jet in the beginning, high concentration appears near gas source and wall where jet momentum is strong. With the jet weakening, stable light gas layer without recirculating eddy is established by buoyancy. Transient reversed helium distribution appears due to natural convection resulted from wall cooling, which delays the stratification. It is necessary to concern about hydrogen accumulation in lower space under the containment external cooling strategy. From the perspective of experiment design, measurement point should be set at the height of connecting pipe and near the wall for stratification stability criterion and impinging jet modelling validation.

• Nuclear Engineering and Technology
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• v.54 no.5
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• pp.1549-1559
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• 2022

As an accident tolerant fuel (ATF) concept, oxide dispersion strengthened Zircaloy-4 (ODS Zry-4) cladding has been developed to enhance the mechanical properties of cladding using laser processing technology. In this study, a simulation technique was established to investigate the mechanical properties and effects of Y₂O₃ particles for the ODS Zry-4. A 3D representative volume element (RVE) model was developed considering the parameters of the size, shape, distribution and volume fraction (VF) of the Y₂O₃ particles. From the 3D RVE model, the Young's modulus, coefficient of thermal expansion (CTE) and creep strain rate of the ODS Zry-4 were effectively calculated. It was observed that the VF of Y₂O₃ particles had a significant effect on the aforementioned mechanical properties. In addition, the predicted properties of ODS Zry-4 were applied to a simulation model to investigate cladding deformation under a transient condition. The ODS Zry-4 cladding showed better performance, such as a delay in large deformation compared to Zry-4 cladding, which was also found experimentally. Accordingly, it is expected that the simulation approach developed here can be efficiently employed to predict more properties and to provide useful information with which to improve ODS Zry-4.