• The Journal of Korean Society of Virology
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• v.29 no.1
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• pp.33-38
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• 1999

Retroviral vector provide a highly efficient method for gene transfer into eukaryotic cells. This vector system can be divided into two components; the retroviral vector itself and the retroviral packaging cell line. The key improvement in the design of these two components are, focused on two aspects; the reduction of helper virus production and high titer-virus. We used PA317 for retrovirus packaging cell line, for its high producibility of viral titer. To test the ability of the vectors to generate high titer-virus, we have chosen four different retroviral vectors; LN, LNSX, LNCX and LXSN. To test easily the viral titer, we have made recombinant construction with CD4 and CD8, checked their viral titer and stained their surface expression. LXSN which contain SV40 early promoter in front of neo gene showed best results in viral transient transfection assay, dot blot assay and surface expression. In addition, recombinant containing CD8 generally showed much higher viral titration and surface expression than CD4.

• Journal of KSNVE
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• v.10 no.6
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• pp.1048-1058
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• 2000

Thipaper presents a methodology to suppress the vibration of thin rectangular plate clamped all edges using piezo-ceramic material as actuators and sensors. Dynamic characteristics of the structure bonded with distributed actuators/sensors are identified by the Multi-Input Multi-Output (MIMO) frequency domain modeling technique based on the experimental data. Hybrid control scheme is adopted and feedback controller is designed by LQG(Linear Quadratic Gaussian). Feedforward controller is adapted by multiple filtered -$x$ LMS(least mean square) algorithm. Experiment result demonstrates the effective reduction of the vibration label for both the transient and persistent external disturbances.

• Nuclear Engineering and Technology
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• v.44 no.4
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• pp.393-404
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• 2012

After the Fukushima nuclear accident in 2011, there has been increasing concern regarding severe accidents in nuclear facilities. Severe accident scenarios are difficult for operators to monitor and identify. Therefore, accurate prediction of a severe accident is important in order to manage it appropriately in the unfavorable conditions. In this study, artificial intelligence (AI) techniques, such as support vector classification (SVC), probabilistic neural network (PNN), group method of data handling (GMDH), and fuzzy neural network (FNN), were used to monitor the major transient scenarios of a severe accident caused by three different initiating events, the hot-leg loss of coolant accident (LOCA), the cold-leg LOCA, and the steam generator tube rupture in pressurized water reactors (PWRs). The SVC and PNN models were used for the event classification. The GMDH and FNN models were employed to accurately predict the important timing representing severe accident scenarios. In addition, in order to verify the proposed algorithm, data from a number of numerical simulations were required in order to train the AI techniques due to the shortage of real LOCA data. The data was acquired by performing simulations using the MAAP4 code. The prediction accuracy of the three types of initiating events was sufficiently high to predict severe accident scenarios. Therefore, the AI techniques can be applied successfully in the identification and monitoring of severe accident scenarios in real PWRs.

• ETRI Journal
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• v.32 no.1
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• pp.33-43
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• 2010

The increasing demand for non-sinusoidal currents affects the quality of distribution networks. Harmonic detection is a crucial step in the cancellation of those components by active power filters. In this paper, the discrete cosine transform (DCT) is compared with different implementations based on Fourier transforms, demonstrating their equivalences and the advantages provided by the former. We demonstrate that the phase error in the presence of grid frequency deviations and the transient length are reduced by half in comparison to the discrete Fourier transform. A novel algorithm is developed to provide frequency adaptation to the DCT, taking advantage of its good features. The window width is adjusted in real time according to the actual value of the grid fundamental frequency by means of a phase-locked loop. A technique based on dithering is employed to overcome the limitation caused by the truncation of the window number of samples, so the frequency resolution is enhanced. The theoretical approach is verified by simulated and experimental results.

• Nuclear Engineering and Technology
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• v.52 no.10
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• pp.2183-2195
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• 2020

A mathematical model for the flowrate and rotation speed of RCP during idling was established. The numerical calculation method and dimensionless method were used to analyze the flow, head, torque and pressure and speed changes under idle conditions. Regularity, using the Q criterion vortex identification judgment method combined with surface flow spectrum morphology analysis to diagnose the vortex dynamic characteristics on RCP blade. On impeller blade, there is two oscillations in the pressure ratio on pressure surface in blade outlet region. The velocity on the suction surface is two times more oscillating than the inlet of blade, and there is an intersection with the velocity ratio curve on pressure surface. On blade of guide vane, the pressure ratio increases along the inlet to outlet direction, and the speed ratio decreases with the increase of idle time. There is a vortex that rotates counterclockwise on the suction surface, and the streamline on the suction surface of blade is subjected to the entrainment and blocking action of the vortex creates a large reverse flow in the main flow region. There are two vortices at the outlet of guide vane suction side and the vortices are in opposite directions.

• Animal cells and systems
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• v.16 no.4
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• pp.289-294
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• 2012

Histone methylation has diverse functions including transcriptional regulation via its lysine or arginine residue methylation. Studies indicate that deregulation of histone methylation is linked to human cancers including leukemia. Histone H3K27 methyltrnasferase response element II binding protein (RE-IIBP), as a transcriptional repressor to target gene IL-5, interacts with HDAC and is over-expressed in leukemia patient samples. In this study, we have identified that hematopoiesis-related genes GATA1 and HOXA9 are down-regulated by RE-IIBP in K562 and 293T cells. Transient reporter analysis revealed that GATA1 transcription was repressed by RE-IIBP. On the other hand, HOXA9 and PBX-related homeobox gene MEIS1 was up-regulated by RE-IIBP. These results suggest that RE-IIBP might have a role in hematopoiesis or leukemogenesis by regulating the transcription of target genes, possibly via its H3K27 methyltransferase activity.

• Journal of Power Electronics
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• v.4 no.2
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• pp.102-111
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• 2004

This paper is concerned with a deadbeat current control implementation of shunt-type three-phase active power filter (APF). Although the one-dimensional deadbeat control method can attain time-optimal response of APF compensating current, one sampling period is actually required fur its settling time. This delay is a serious drawback for this control technique. To cancel such a delay and one more delay caused by DSP execution time, the desired APF compensating current has to be predicted two sampling periods ahead. Therefore an adaptive predictor is adopted for the purpose of both predicting the control error of two sampling periods ahead and bringing the robustness to the deadbeat current control system. By adding the adaptive predictor output as an adjustment term to the reference value of half a source voltage period before, settling time is made short in a transient state. On the other hand, in a steady state, THD (total harmonic distortion) of the utility grid side AC source current can be reduced as much as possible, compared to the case that ideal identification of controlled system could be made.

• International Journal of Precision Engineering and Manufacturing
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• v.6 no.4
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• pp.37-43
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• 2005

This paper presents a hybrid control algorithm for the active noise control in the rectangular enclosure using an active/passive foam actuator. The hybrid control composes of the adaptive feedforward with feedback loop in which the adaptive feedforward control uses the well-known filtered-x LMS(least mean square) algorithm and the feedback loop consists of the sliding mode controller and observer. The hybrid control has its robustness for both transient and persistent external disturbances and increases the convergence speed due to the reduced variance of the jiltered-x signal by adding the feedback loop. The sliding mode control (SMC) is used to incorporate insensitivity to parameter variations and rejection of disturbances and the observer is used to get the state information in the controller deign. An active/passive smart foam actuator is used to minimize noise actively using an embedded PVDF film driven by an electrical input and passively using an absorption-foam. The error path dynamics is experimentally identified in the form of the auto-regressive and moving-average using the frequency domain identification technique. Experimental results demonstrate the effectiveness of the hybrid control and the feasibility of the smart foam actuator.

• Current Industrial and Technological Trends in Aerospace
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• v.8 no.1
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• pp.131-139
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• 2010

Avionics technologies have been developed with a development of an airplane since 1903. Historically, radio communication was started in 1910's, radio navigations in 1920's and autopilot was applied first in 1930's. Glass cockpit was initiate on MD-80 in 1979 first and now spreaded widely and similar with GPS navigation. Avionics in modern aircraft has a great deal of importance in view of flight safety and maintaining comfortableness. As avionics develops, so do the certification technologies. This paper introduces update avionics certification technologies developed recently.

• Nuclear Engineering and Technology
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• v.37 no.1
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• pp.25-78
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• 2005

As a key and core knowledge for the design of various types of nuclear reactors, the discipline of reactor physics has been advanced continually in the past six decades and has led to a very sophisticated fabric of analysis methods and computer codes in use today. Notwithstanding, the discipline faces interesting challenges from next-generation nuclear reactors and innovative new fuel designs in the coming. After presenting a brief overview of important tasks and steps involved in the nuclear design and analysis of a reactor, this article focuses on the currently-used design and analysis methods, issues and limitations, and current activities to resolve them as follows: (1) Derivation of the multi group transport equations and the multi group diffusion equations, with representative solution methods thereof. (2) Elements of modem (now almost three decades old) diffusion nodal methods. (3) Limitations of nodal methods such as transverse integration, flux reconstruction, and analysis of UO2-MOX mixed cores. Homogenization and related issues. (4) Description of the analytic function expansion nodal (AFEN) method. (5) Ongoing efforts for three-dimensional whole-core heterogeneous transport calculations and acceleration methods. (6) Elements of spatial kinetics calculation methods and coupled neutronics and thermal-hydraulics transient analysis. (7) Identification of future research and development areas in advanced reactors and Generation-IV reactors, in particular, in very high temperature gas reactor (VHTR) cores.