• Journal of Microbiology and Biotechnology
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• 제22권1호
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• pp.50-57
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• 2012

Phospholipase C-${\gamma}l$ (PLC-${\gamma}l$) expression is associated with cellular transformation. Notably, PLC-${\gamma}$ is up-regulated in colorectal cancer tissue and breast carcinoma. Because exotoxins released by Clostridium botulinum have been shown to induce apoptosis and promote growth arrest in various cancer cell lines, we examined here the potential of Clostridium difficile toxin A to selectively induce apoptosis in cells transformed by PLC-${\gamma}l$ overexpression. We found that PLC-${\gamma}l$-transformed cells, but not vector-transformed (control) cells, were highly sensitive to C. difficile toxin A-induced apoptosis and mitotic inhibition. Moreover, expression of the proapoptotic Bcl2 family member, Bim, and activation of caspase-3 were significantly up-regulated by toxin A in PLC-${\gamma}l$-transformed cells. Toxin A-induced cell rounding and paxillin dephosphorylation were also significantly higher in PLC-${\gamma}l$-transformed cells than in control cells. These findings suggest that C. difficile toxin A may have potential as an anticancer agent against colorectal cancers and breast carcinomas in which PLC-${\gamma}l$ is highly up-regulated.

• 대한금속재료학회지
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• 제50권6호
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• pp.455-462
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• 2012

Al-Si coated boron steel and Zn coated DP steel plates were laser-welded to manufacture a Tailor Welded Blank (TWB) for a car body frame. Hot-stamping heat treatment ($900^{\circ}C$, 5 min) was applied to the TWB weld, and the microstructural change and transformation mechanism were investigated in the Al-rich area near the bond line of the Al-Si coated steel side. There was Al-rich area with a single phase, $Fe_3(Al,Si)$, which was transformed to ${\alpha}-Fe$ (Ferrite) after the heat treatment. It could be explained that the $Fe_3(Al,Si)$ phase was transformed to ${\alpha}-Fe$ during heat treatment at $900^{\circ}C$ for 5 min and the resultant ${\alpha}-Fe$ phase was not transformed by rapid cooling. Before the heat treatment, the microstructures around the $Fe_3(Al,Si)$ phase consisted of martensite, bainite and ${\alpha}-Fe$ while they were transformed to martensite and ${\delta}-Fe$ after the heat treatment. Due to the heat treatment, Al was diffused to the $Fe_3(Al,Si)$ and this resulted in an increase of Al content to 0.7 wt% around the Al-rich area. If the weld was held at $900^{\circ}C$ for 5 min it was transformed to a mixture of austenite (${\gamma}$) and ${\delta}-Fe$, and only ${\gamma}$ was transformed to the martensite by water cooling while the ${\delta}-Fe$ was remained unchanged.

• Journal of Ginseng Research
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• 제45권1호
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• pp.163-175
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• 2021

Background: Ginsenosides, which have strong biological activities, can be divided into polar or less-polar ginsenosides. Methods: This study evaluated the phytochemical diversity of the saponins in Panax ginseng (PG) root, American ginseng (AG) root, and Panax notoginseng (NG) root; the stem-leaves from Panax ginseng (SPG) root, American ginseng (SAG) root, and Panax notoginseng (SNG) root as well as the saponins obtained following heating and acidification [transformed Panax ginseng (TPG), transformed American ginseng (TAG), transformed Panax notoginseng (TNG), transformed stem-leaves from Panax ginseng (TSPG), transformed stem-leaves from American ginseng (TSAG), and transformed stem-leaves from Panax notoginseng (TSNG)]. The diversity was determined through the simultaneous quantification of the 16 major ginsenosides. Results: The content of ginsenosides in NG was found to be higher than those in AG and PG, and the content in SPG was greater than those in SNG and SAG. After transformation, the contents of polar ginsenosides in the raw saponins decreased, and contents of less-polar compounds increased. TNG had the highest levels of ginsenosides, which is consistent with the transformation of ginseng root. The contents of saponins in the stem-leaves were higher than those in the roots. The transformation rate of SNG was higher than those of the other samples, and the loss ratios of total ginsenosides from NG (6%) and SNG (4%) were the lowest among the tested materials. In addition to the conversion temperature, time, and pH, the crude protein content also affects the conversion to rare saponins. The proteins in Panax notoginseng allowed the highest conversion rate. Conclusion: Thus, the industrial preparation of less-polar ginsenosides from SNG is more efficient and cheaper.

• Asian-Australasian Journal of Animal Sciences
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• 제23권2호
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• pp.246-252
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• 2010

In order to improve the availability of phytase and probiotics together, a phytase gene from Aspergillus ficuum has been expressed in Lactobacillus. In this study, the transformed Lactobacillus with phytase gene was fed to pigs to determine its effect on pig production, feed conversion and gut microbes. Forty eight, 60-day-old, castrated pigs (Duroc${\times}$Landrace${\times}$Pietrain) were assigned to 6 groups, 8 pigs for each group. Group 1 was the control, group 2 was added with chlortetracycline (500 mg/kg), group 3 was added with the transformed Lactobacillus (500 mg/kg) with 20% (w/w) of calcium monohydrogen phosphate (CMP, $CaHPO_{4}$) removed, group 4 was added with the natural Lactobacillus (500 mg/kg) with 20% (w/w) of CMP removed, group 5 was added with the transformed Lactobacillus (500 mg/kg) with 40% (w/w) of CMP removed, group 6 was added with phytase (500 mg/kg) with 40% (w/w) of CMP removed. The results showed: i) the average daily gain (ADG) was improved in groups 2, 3 and 4 (p<0.05); ii) the diarrhea rates in the groups added with Lactobacillus were lower than in the other groups (p<0.05), in which the transformed Lactobacillus had more effect on reducing digestive disease; iii) the transformed Lactobacillus was most effective in improving the digestibilities of crude protein (CP), calcium (Ca), phosphorus (P), compared with the other groups (p<0.05); iv) Lactobacillus could increase lactic acid bacterium number and ammonia concentrations, and decrease pH values and E. coli number in pig feces (p<0.05); v) the phytase activity in the feces of pigs fed with the transformed Lactobacillus was 133.32 U/g, which was higher than in group 4 (9.58 U/g, p<0.05), and was almost the same as group 6 (135.94 U/g); vi) the transformed Lactobacillus could increase serum concentrations of IgA, triglyceride, and glutamic oxaloacetic transaminase activity (p<0.05), and had no significant effect on other serum indexes (p>0.05).

• Journal of Acupuncture Research
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• 제25권5호
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• pp.17-25
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• 2008

Objectives : The authors performed this study to further understand Saam acupuncture treatment in an aspect of the use of two patterns i.e. fixed- and transformed-pattern. Methods : The authors did documentary survey based on Do Hae Kyo Kam Sa Am Do In Chim Beop. 1. We investigated the frequency of the use of fixed- and transformed-pattern as well as the examples of use. 2. We surveyed related literatures and classified transformed-pattern. We did study on the principles of prescriptions of fixed- and transformed-pattern and researched the utilization. Results and Conclusions : 1. Fixed-pattern occupys 35.3% and transformed-pattern takes much more part. 2. Fixed-pattern is based on Nangyoung 69nan, and has the prescription of two enhancement and two inhibition by adaptation of Sangsaengsanggeuk(相生相剋) into self meridian and other meridian, and suggests the importance of the order of needling. 3. There are main points and assistant points in tonifying and suppressing of Fixed-pattern, accordingly, it has a system like Gunshinjwasa(君臣佐使). 4. Transformed-pattern is classified into following 3 types: first, the use of Nangyoung 75nan; second, the substitution of Junghuyl(井穴) with Hyunghyul(滎穴) or Haphyul(合穴) according to Nangyoung 73nan; third, the substitution of the same Osuhyul(五輸穴) in other meridians.

• Communications for Statistical Applications and Methods
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• 제14권1호
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• pp.141-146
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• 2007

In this paper, we consider a Box-Cox transformed threshold GARCH(1,1) process and find a sufficient condition under which the process is geometrically ergodic and has the ${\beta}$-mixing property with an exponential decay rate.

• 대한수학회논문집
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• 제10권3호
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• pp.621-632
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• 1995

The expression of the density of the thinnest covering of $R^n$ shall be transformed into a more 'available' form, and some applications of the transformed expression shall be introduced.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.117.2-118
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• 2003

Sample size calculations plays an important role in a bioequivalence trials and is determined by considering power under the alternative hypothesis. The regulatory guideline recommends that $2{\times}2$ crossover design is conducted and raw data is log-transformed for statistical analysis. In this paper, we discuss the sample size calculation in $2{\times}2$ crossover design with the log-transformed data.

• Journal of the Korean Data and Information Science Society
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• 제28권1호
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• pp.143-152
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• 2017

블럭내의 완전랜덤화 제약은 하나의 블럭이 여러 실험구로 분할되는 분할요인모형으로 해결할 수 있다. 본 연구는 $3{\times}3$ 분할요인모형에서 두 주요인 및 하나의 블럭이 모두 고정일 경우에는, 실제로 존재하는 효과크기가 작을수록 혹은 검정대상의 요인효과 크기보다 검정대상 이외의 효과들의 크기가 상대적으로 작을수록 주구요인효과 및 세구요인효과 검정을 위한 순위변환 통계량의 검정력은 기존의 모수적 통계량의 검정력보다 뛰어남을 알 수 있다. 또한 모집단 모형의 오차항이 지수분포 및 이중지수분포일 때 효과크기 및 효과구성유형에 상관없이 거의 모든 상황하에서 순위변환 통계량의 검정력이 모수적 통계량의 검정력보다 상대적으로 높은 우위를 보이며, 정규분포 및 균일분포하에서는 상당히 유사한 수준을 나타낸다. 한편 두 주요인은 고정이나 하나의 블럭이 랜덤일 경우에는, 두 주요인 및 블럭이 모두 고정일 경우보다 모수적 통계량 및 순위변환 통계량의 검정력은 각각 낮은 수준을 보인다. 특히 주구요인효과 검정보다 세구요인효과 검정을 위한 모수적 통계량 및 순위변환 통계량의 검정력이 다소 낮은 수준임을 보이지만, 순위변환 통계량의 검정력은 모수적 통계량의 검정력에 비하여 높은 상대적 검정력 우위를 나타낸다.

• 감성과학
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• 제5권2호
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• pp.23-28
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• 2002

본 연구에서는 의류용 직물의 소리와 변환색채의 특성을 분류하고, 역학특성과의 관계분석을 통해 직물의 역학적 특성과 소리 그리고 변환색채와의 관계를 상관분석하고자 하였다. 다양한 의류용 직물의 스치는 소리를 녹음한 후 소리의 물리량으로 군집분석을 하였으며, Okamoto와 Mori의 색채변환 프로그램을 이용하여 직물의 소리를 색채로 변환하였다. 직물의 소리는 각각 silky, crispy, paper-like, worsted, flax의 다섯 그룹으로 나뉘었다. Silky는 매끄럽고 부드러운 견직물 등으로 구성되었으며, LPT(소리의 음압), $\Delta$f, ARC값이 군집 중 가장 낮아 심리 음향학적 loudness(z)와 sharpness(z)가 제일 낮은 조용한 직물들로 나타났다. 변환된 색채는 홍색 계열이었으며 색채 빈도수가 가장 많았다. raper-like는 뻣뻣하고 파삭파삭한 직물들로 LPT, $\Delta$f ARC값이 군집 중 가장 높아 loudness와 sharpness가 높은 시끄러운 직물들로 나타났다. Worsted는 모직물과 모직물 느낌의 직물들로 LPT, $\Delta$f, loudness값이 paper-like다음으로 높은 값을 보였으며 sharpness는 유사했다. Paper-like와 worsted의 변환된 색채는 청색 계열이 주류를 이루었으며 매우 낮은 색채 빈도수를 나타냈다. Crispy는 표면이 까실까실한 느낌의 직물들로 loudness(z)와 sharpness(z)의 값은 높지 않았으나 변환된 색채는 다양한 색채계열과 빈도수를 나타냈다.