• Journal of Microbiology and Biotechnology
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• 제4권1호
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• pp.7-12
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• 1994

A yeast strain secreting glucoamylase was transformed with an expression vector (pMS12) containing the promoter of yeast alcohol dehydrogenase I gene ADC1, mouse salivary $\alpha$-amylase cDNA, and a segment of yeast $21\mu m$ plasmid. The transformed strain could produce ethanol from starch (4%, w/v) through a direct one-step process with the conversion efficiency of 93.2%, during 5 days of fermentation, while the original, untransformed strain exhibited a conversion efficiency of 38.1% under the same condition. When the regulatory site of the ADC1 promoter region was removed, the production of ethanol increased to 29~37% in the presence of exogenous 3%(v/v) ethanol in the fermentation medium.

• 대한수학회논문집
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• 제20권4호
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• pp.695-702
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• 2005

We study the transformed measures with respect to the real parameters of a self-similar measure on a self-similar Can­tor set to give a simple proof for some result of its multifractal spectrum. A transformed measure with respect to a real parameter of a self-similar measure on a self-similar Cantor set is also a self­similar measure on the self-similar Cantor set and it gives a better information for multifractals than the original self-similar measure. A transformed measure with respect to an optimal parameter deter­mines Hausdorff and packing dimensions of a set of the points which has same local dimension for a self-similar measure. We compute the values of the transformed measures with respect to the real parameters for a set of the points which has same local dimension for a self-similar measure. Finally we investigate the magnitude of the local dimensions of a self-similar measure and give some correlation between the local dimensions.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권2호
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• pp.105-109
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• 2006

A transformed hairy root clone of Scutellaria baicalensis was established following infection with Agrobacterium rhizogenes ATCC15834. Three root clones of S baicalensis were selected by growth habit and baicalin content. The most active strain-the SR-03 clone-was examined for its growth and baicalin content under various culture conditions. The root growth and baicalin content were maximized in a Schenk and Hildebrandt medium supplemented with 4 and 6% sucrose, respectively. The accumulation of baicalin in transformed hairy roots was enhanced through exposure to various elicitors. Elicitation was attained by the addition of methyl jasmonate, salicylic acid, and various concentrations of fungal cell wall elicitors to the medium. The accumulation of baicalin in the elicited cultures ranged from 10.5 to 18.3 mg/g dry weight of the roots, which was 1.5- to 3-fold the amount attained in controls.

• Communications for Statistical Applications and Methods
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• 제16권6호
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• pp.891-901
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• 2009

이 논문에서는 GARCH 모형에서 가정한 오차향의 분포에 근접하도록 자료를 변환하고 변환된 자료를 이용하여 모수와 예측구간을 구한 후 다시 역변환을 통해 원래의 척도에서의 VaR을 계산하는 방법에 대해 알아본다. KOSPI와 KOSDAQ 수익률을 이동시키며 VaR을 계산하고 이들 VaR의 포함확률을 계산하여 병목수준에 얼마나 근접하는지를 알아봄으로써 변환-역변환 방법과 변환을 적용하지 않는 방법의 결과를 비교해 본다.

• 전자공학회논문지S
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• 제35S권5호
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• pp.59-67
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• 1998

This paper propose a new vector-quantizer design method for coefficients of wavelet transformed images. In conventional wavelet transform, it is quite often to employ wavelet transformed coefficients, not containing images to be encoded, as training sequences for designing a vector-quantizer. This method has a serious drawback ; it is not known how to find a proper set of training images. This paper investigates characteristics of images that should be considered in the design of vector-quantizers for wavelet transformed images. Besides the statistical parameters such as correlation and standard deviation, edge components are shown to characterise wavelet transform images. Training sequences established in accordance with the above knowledge are used in the design of quantizers having guaranteed range of applicable images. Results of computer simulations are shown to demonstrate the effectiveness of the proposed method.

• 한국초지조사료학회지
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• 제21권3호
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• pp.145-150
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• 2001

This study was conducted to obtain the transformed birdsfoot trefoil (Lotus corniculatus L.) plants with BcHSP17.6 gene using Agrobacterium turnefaciens LBA4404 and we confirmed transformed gene from the regenerated birdsfoot trefoil plants. The expression vector, pBKH4 vector, harboring BcHSP17.6 gene was used for production of transgenic birdsfoot trefoil plants. The callus of birdsfoot trefoil was cocultivated with Agrobacteriurn turnefaciens and transformed calli were selected on kanamycin-containing SH-kc medium to regenerate into plants. The transformed birdsfoot trefoil plants were produced 4 momths after cultivation on BOi2Y medium. The transgenic birdsfoot trefoil plants were analyzed by isolation of genomic DNA and genomic Southern hybridization using a -32P labelled BcHSPl7.6 fragments. (Key words : Birdsfoot trefoil, Transgenic plant. BcHSP17.6 gene, Callus induction, Plant regeneration)

• Kyungpook Mathematical Journal
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• 제62권3호
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• pp.557-581
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• 2022

The anisotropic-diffusion convection equation with exponentially variable coefficients is discussed in this paper. Numerical solutions are found using a combined Laplace transform and boundary element method. The variable coefficients equation is usually used to model problems of functionally graded media. First the variable coefficients equation is transformed to a constant coefficients equation. The constant coefficients equation is then Laplace-transformed so that the time variable vanishes. The Laplace-transformed equation is consequently written as a boundary integral equation which involves a time-free fundamental solution. The boundary integral equation is therefore employed to find numerical solutions using a standard boundary element method. Finally the results obtained are inversely transformed numerically using the Stehfest formula to get solutions in the time variable. The combined Laplace transform and boundary element method are easy to implement and accurate for solving unsteady problems of anisotropic exponentially graded media governed by the diffusion convection equation.

• International Journal of Industrial Entomology and Biomaterials
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• 제2권1호
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• pp.19-26
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• 2001

Transformed Spodoptera frugiperda (Sf9) cells expressing baculovirus very late factor (VLF-1) were constructed by using Autograha nuclear polyhedrosis virus (AcNPV) immediate earthy gene (ie1). Neomycin-resistance gene as a selectable marker was introduced under the control of AcNPV ie1 promoter, and Bombyx mori nuclear polyhedrosis (BmNPV-K1) vlf-1 gene was introduced under the control of the Drosophila heat shock protein gene (hspr70) promoter to yield dual expression plasmid with two independent transcription units. It was transfected into Sf9 cells and cell clones expressing vlf-1 were selected by G4l8 treatment. Genomic DNA from transformed cells was isolated and integration of AcNPV iel harboring vlf-1 was confirmed by PCR using AcNPV iel-specific primers and Southern blot analysis. The transformed cells expressing VLF-1 in an infection-independent manner expressed foreign gene product of recombinant baculovirus in the earlier stage of infection compared with control Sf9 cells. These results suggest the possible to develop highly efficient transformed insect cells for baculovirus expression vector system.

• 한국환경복원기술학회지
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• 제9권2호
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• pp.1-15
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• 2006

As the imports of foreign agricultural products are liberalized and the consumption of agricultural products declines, abandoned rice paddy fields continues to rise. However, such abandoned rice paddy fields has not been precisely surveyed yet. In this backdrop, a necessity to develop technology to utilize such abandoned rice paddy fields has emerged. Utilization of abandoned rice paddy fields as wetlands may be a good example. This study aimed to survey the current status and characteristics of wetlands created within abandoned rice paddy fields by selecting abandoned rice paddy fields throughout the nation and conducting field surveys on the sites that had transformed into wetlands. The abandoned rice paddy fields almost transformed into wetland and the types of wetlands transformed from abandoned rice paddy fields were mainly Inland/Moutain/Depression/Abandoned rice paddy fields/Marsh/Phragmites communis community and Inland/Moutain/Depression/Abandoned rice paddy fields/Swamp/Salix koreensis community. Abandoned rice paddy fields that had transformed into wetlands was depending heavily on waterways for water supply than other reservoirs and lakes do. Abandoned rice paddy fields transformed into wetlands was most observed in mountainous area. Abandoned rice paddy fields are because agricultural land is no longer profitable due to international and social changes and is not cultivated as government policy. Wetland period and dimension originated from abandoned rice paddy fields are very various and its surrounding land its mostly forest and the next largest follow roads and rural community. The abandoned rice paddy fields transformed into wetlands is mostly deserted currently. Despite their value as wetlands, no restoration and utilization efforts are made in Korea today. Therefore, it is imperative to conduct a precise current status survey on these areas and introduce management and restoration plans at the government level in the case of important habitats.

• Journal of Microbiology and Biotechnology
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• 제11권2호
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• pp.186-192
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• 2001

A primary culture of human fetal hepatocytes was obtained through a therapeutic abortion process at 26 weeks of gestation period. More than $10^8$ cells were seeded on a plastic plate. These hepatocytes were infected with hepatitis B virus (HBV). The HBV was purified from serum of one chronic HBV carrier. Transformed hepatocytes were subcultured in a 10% FBS-supplemented medium. The morphology of the transformed cell was epithelial-like. The cells from the first pass showed signs of early proliferation and had a latent period of more than 3 months after 6-7 passages. After the rest period, the transformed cell proliferated actively and they were subcultured every three days. Transformed hepatocytes were characterized by detection of the HBV transcript by RT-PCR. The secretion of virions from transformed cells was investigated by PCR with the cell medium. Two types of virions secreted into the culture medium were examined by using the transmission electron microscope. Another approach to study the secretion of virions in to culture medium was carried out with HBV antibody. HBsAg was detected in the culture medium of transformed cells using ELISA and Western blot analyses. These data suggested that the human fetal hepatocyte cell line has been established by infection of HBV, in which this cell line secreted viral particles into the culture medium.