• Title/Summary/Keyword: transformed

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Functional Expression of Recombinant Tumstatin in Stably Transformed Drosophila melanogaster S2 Cells

  • Sohn, Bong-Hee;Kim, Yong-Soon;Kang, Pil-Don;Lee, Sang-Uk;Lee, Jong-Min;Chung, In-Sik
    • Proceedings of the Korean Society of Sericultural Science Conference
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    • 2003.10a
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    • pp.147-148
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    • 2003
  • The purpose of this experiment is to confirm whether the recombinant tumstatin revealed from the stably transformed Drosophila melanogaster S2 cells has in vitro capacity. Materials and Methods : Materials - Cell line : Drosophila melanogaster Schneider 2 (S2) cells - vector pMT/BiP/V5-His and pCoHygro (Invitrogen) (omitted)

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A Study on a Method of Determining the Most Vital Arc in the Maximum Flow Problem (최대유통문제에서 MVA를 결정하는 방법에 관한 연구)

  • 정호연
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.19 no.40
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    • pp.263-269
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    • 1996
  • The most vital arc in the maximum flow problem is that arc whose removal results in the greatest reduction in the value of the maximal flow between a source node and a sink node. This paper develops an algorithm to determine such a most vital arc(MVA) in the maximum flow problem. We first define the transformed network corresponding In a given network in order to compute the minimal capacity for each candidate arc. The set of candidate arcs for a MVA consists of the arcs whose flow is at least as greate as the flow over every arc in a minimal cut As a result, we present a method in which the MVA is determined more easily by computing the minimal capacity in the transformed network. The proposed method is demonstrated by numerical example.

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Agrobacterium-mediated transformation of Lycopersicon esculentum (cv. MicroTom) with two pathogen-induced hot pepper transcription factors

  • Seong, Eun-Soo;Oh, Sang-Keun;Eunsook Chung;Park, Doil
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.79.1-79
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    • 2003
  • Two pathogen-induced hot pepper transcription factors (CaNACl and CapIfl) were introduced into‘MicroTom’tomato by Agrobacterium tumefaciens-mediated transformation. We used to nptII containing kanamycin resistance gene as a selection marker. Both transformed and non-transformed plants were transferred to pot after rooting test in vitro. To approximate the levels of caNACl transcript in leaves of wild-type and transgenic plants, RNA blots were hybridized with double-stranded full-length CaNACl probe at moderate stringency, Although the relative signal strength for hybridization fluctuated among the samples on different blots, transgenic plant lines N-1, N-2 and N-3 consistently displayed increased levels of CaNACl transcript relative to other transgenic lines and wild-type plants. Of all the transgenic lines examined, line N-7 had the least amount of CaNACl transcript. Role of these transcription factors in pathogen defense will be examined by overexpression in tomato.

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Video Segmentation and Key frame Extraction using Multi-resolution Analysis and Statistical Characteristic

  • Cho, Wan-Hyun;Park, Soon-Young;Park, Jong-Hyun
    • Communications for Statistical Applications and Methods
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    • v.10 no.2
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    • pp.457-469
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    • 2003
  • In this paper, we have proposed the efficient algorithm that can segment the video scene change using a various statistical characteristics obtained from by applying the wavelet transformation for each frames. Our method firstly extracts the histogram features from low frequency subband of wavelet-transformed image and then uses these features to detect the abrupt scene change. Second, it extracts the edge information from applying the mesh method to the high frequency subband of transformed image. We quantify the extracted edge information as the values of variance characteristic of each pixel and use these values to detect the gradual scene change. And we have also proposed an algorithm how extract the proper key frame from segmented video scene. Experiment results show that the proposed method is both very efficient algorithm in segmenting video frames and also is to become the appropriate key frame extraction method.

Validation Comparison of Credit Rating Models Using Box-Cox Transformation

  • Hong, Chong-Sun;Choi, Jeong-Min
    • Journal of the Korean Data and Information Science Society
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    • v.19 no.3
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    • pp.789-800
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    • 2008
  • Current credit evaluation models based on financial data make use of smoothing estimated default ratios which are transformed from each financial variable. In this work, some problems of the credit evaluation models developed by financial experts are discussed and we propose improved credit evaluation models based on the stepwise variable selection method and Box-Cox transformed data whose distribution is much skewed to the right. After comparing goodness-of-fit tests of these models, the validation of the credit evaluation models using statistical methods such as the stepwise variable selection method and Box-Cox transformation function is explained.

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AN IMPROVED COMPUTATION OF COMPONENT CENTERS IN THE DECREE-n BIFURCATION SET

  • Geum, Young-Hee;Kim, Young-Ik
    • Journal of applied mathematics & informatics
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    • v.10 no.1_2
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    • pp.63-73
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    • 2002
  • The governing equation locating component centers in the degree-n bifurcation set is a polynomial with a very high degree and its root-finding lacks numerical accuracy. The equation is transformed to have its degree reduced by a factor(n-1). Newton's method applied to the transformed equation improves the accuracy with properly chosen initial values. The numerical implementation is done with Maple V using a large number of computational precision digits. Many cases are studied for 2 $\leq$ n $\leq$ 25 and show a remarkably improved computation.

Prediction of effective stiffness on short fiber reinforced composite materials (단섬유 복합재료의 탄성계수 예측)

  • 임태원;한경섭
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.15 no.2
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    • pp.611-617
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    • 1991
  • Effective stiffness of short fiber composite with a three-dimensional random orientation of fibers is derived theoretically and compared with available experimental data. The laminate analogy and transformed laminate analogy are used for modulus prediction of 2-D and 3-D random composites, respectively. The effective stiffness of random oriented fiber composite can be expressed in terms of longitudinal and transverse stiffnesses of unidirectional composites. The result of transformed laminate analogy is more accurate than other approaches such as, Christensen-Waals equational and Lavengood-Goettler equation, etc. Also the effective properties of random oriented fiber composite can be expressed in terms of fiber and matrix properties such as elastic modulus, shear modulus and Poisson's ratio.

Polymerization of Tetraethoxysilane by Using Remote Argon/dinitrogen oxide Microwave Plasma

  • Chun, Tae-Il;Rossbach, Volker
    • Textile Coloration and Finishing
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    • v.21 no.3
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    • pp.19-25
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    • 2009
  • Polymerization of tetraethoxysilane on a glass substrate was investigated by remote microwave plasma using argon with portions of nitrous oxide as carrier gas. Transparent layer like a thickness of 0.5 ${\mu}m$ 3 ${\mu}m$ were obtained, differing in chemical composition, depending on plasma power and treatment time as well as on ageing time. In general the milder the treatment and the shorter the ageing was, the higher was the content of organic structural elements in the layer. We have identified that the chemical structure of our samples composed of mainly Si O and Si C groups containing aliphatics, carbonyl groups. These results were obtained by X ray photon spectroscopy, Fourier transformed infrared spectroscopy, and scanning electron microscope combined with Energy dispersive X ray spectroscopy.

Trend Analysis for Stratospheric Ozone Concentration in the Middle Latitude Northern Hemisphere Using HALOE Data (HALOE 자료를 이용한 중위도 지역의 오존농도 추이분석)

  • Ka, Soo-Hyun;Kwon, Mi-Ra;Oh, Jung-Jin
    • Journal of Korean Society for Atmospheric Environment
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    • v.21 no.4
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    • pp.413-422
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    • 2005
  • The ozone concentration measured by HALOE (Ver 19) from Oct. 1991 to Dec. 2003 is used for analyzing the variation of ozone concentration. The HALOE loaded in UARS is observing several gases in the atmosphere, from 10km to 80km. Fourier analysis of these data in the middle latitude northern hemisphere is reported in this paper. To detect any possible long term trends, the fourier transformed time series was back transformed after removing signals with time periods of less than 6 months. Although the results clearly show the strong annual cycle, it is difficult to show any long term trends from the fourier series. We also compared the ozone volume mixing ratio's from HALOE with that from the ground-based radiometry to evaluate the accuracy of microwave observation at Sookmyung Women's University.

Production of Recombinant Rotavirus Capsid Protein VP7 from Stably Transformed Drosophila melanogaster S2 Cells

  • Park, Jong-Hwa;Chang, Kyung-Hwa;Lee, Youn-Hyung;Kim, Hae-Yeong;Yang, Jai-Myung;Chung, In-Sik
    • Journal of Microbiology and Biotechnology
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    • v.12 no.4
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    • pp.563-568
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    • 2002
  • Stably transformed Drosophila melanogaster 52 cells producing recombinant VP7 were obtained, and recombinant VP7 expression was confirmed by Western blot analysis. The molecular weight of recombinant VP7 expressed in 52 cells was approximately 35.5 kDa, and 75% of the total VP7 produced was present in the medium. Recombinant VP7 contained N-linked glycosylated oligosaccharides. Aprotinin, leupeptin, and polyvinylpyrrolidone did not have any noticeable effect on recombinant VP7 production; however, DMSO and sodium butyrate increased its production by 120% and 60%, respectively.