• Title/Summary/Keyword: transformed

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Chip Shape Control using AE Signal in Pure Copper Turning (순동선삭가공에서 AE 신호를 이용한 칩 형상 제어)

  • Oh, Jeong Kyu;Kim, Pyeong Ho;Koo, Joon Young;Kim, Duck Whan;Kim, Jeong Suk
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.23 no.4
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    • pp.330-336
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    • 2014
  • The continuous chip generated in cutting process deteriorates workpiece, tool, and machine tool system. It is necessary to treat this continuous chip in ductile material machining condition for stable cutting. This paper deals with the chip control method using acoustic emission(AE) signal in pure copper turning operation. AE raw signals, root mean square(RMS) signals and wavelet transformed signals measured in turning process are introduced to analysis for chip patterns. With analysis of AE signals, it is obtained that the produced chip patterns are correlated with the specified AE signals which are transformed by fuzzy pattern algorithm. By this experimental investigation, the chip patterns can be classified at significant level in pure copper machining process and controlled from continuous chips to reduced-length stable chips.

Gene Expression in Transformed Higher Plants II. Effect $Ca^{2+}$ on $\beta-Glucan$ Synthetase II Activity and Changes of Peroxidase Pattern in Transformed Potato Tuber (고등 식물의 형질전환에 따른 유전자 발현 II. 형질전환된 감자 괴경의 $\beta-Glucan$ Synthetase II의 활성에 미치는 Ca2+의 효과 및 Peroxidase의 변화 양상)

  • 정현숙
    • Journal of Plant Biology
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    • v.34 no.4
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    • pp.261-266
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    • 1991
  • The hairy root was induced form potato tuber disc by infection of a. rhizogenes. The detection of the agropine and mannopine by paper electrophoresis confirmed that induced hairy root was transformed by A. rhizogenes. The activity of peroxidase was the highest at 5 weeks and isozyme pattern of peroxidase revealed 3 cathodic bands and 2 anodic bands and new C4 band(pI 4.6) was observed at 7 weeks after cultivation in hairy root was isoelectric focusing. To study the effect of Ca2+ on cell wall formation in hairy root, channel blocker of Ca2+ was treated. The activity of $\beta$-glucan synthetase II(GS II) related to cell wall synthesis was inhibited by about 50% in diltiazem and flunarizine treatment than that of control, but stimulated in CaCl2 treatment. Therefore these results showed that Ca2+ might be an effective factor in the cell wall formation. The activity of GS II by NaF treatment was increased by about 30%. This result suggested that the activity GS II is changed through phosphorylation process.

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Transformation of Measured Blasting Vibration Data to Vibration Level by Digital Filter (디지털 필터를 이용한 진동의 변환 연구)

  • Kim, Yong-Kuk;Kim, Sik;Lee, Sang-Eun;Yang, Hyung-Sik
    • Tunnel and Underground Space
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    • v.10 no.3
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    • pp.486-491
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    • 2000
  • Blasting vibrations velocity had been used to design blasting pattern and predict damage-possibility of structures. As vibration levels are currently required to estimate the damage concerning human-body and livestock, so measured vibration velocities must be transformed into vibration levels. In this study, the digital filter program was developed to transform measured data into vibration levels. Simple harmonic vibrations and measured data obtain by Blastmate series (Instantel Inc.) were applied to the digital filter program. As a result, simple harmonic vibrations and measured data were successfully transformed into vibration levels using the developed program. Measured data from any instrument with ASCII output-function will similarly be transformed into level units.

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Transformation of Measured Blasting Vibration Data to Vibration Level by Digital Filter (디지털 필터를 이용한 진동의 변환 연구)

  • Kim, Yong-Kuk;Kim, Sik;Lee, Sang-Eun;Yang, Hyung-Sik
    • Proceedings of the Korean Society for Rock Mechanics Conference
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    • 2000.09a
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    • pp.241-246
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    • 2000
  • Blasting vibrations velocity had been used to design blasting pattern and predict damage-possibility of structures. As vibration levels are currently required to estimate the damage concerning human-body and livestock, so measured vibration velocities must be transformed into vibration levels. In this study, the digital filter program was developed to transform measured data into vibration levels. Simple harmonic vibrations and measured data obtained by Blastmate series (Instantel Inc.) were applied to the digital filter program. As a result, simple harmonic vibrations and measured data were successfully transformed into vibration levels using the developed program. Measured data from any instrument with ASCII output-function will similarly be transformed into level units.

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Chronic Treatment of Ethanol Inhibits Proliferation of Normal Fibroblasts, but Not Oncogenic ras-Transformed Cells

  • Gu, Young-Hwa;Park, Mi-Sun;Jhun, Byung-H.
    • Biomolecules & Therapeutics
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    • v.6 no.4
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    • pp.345-350
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    • 1998
  • The adverse effects of ethanol on cell proliferation have been described for a variety of tissues and cells. In the present study, we investigated whether chronic ethanol intoxication impairs the cell proliferation and DNA synthesis induced by oncogenic $H-ras^{V12}$ - and $v-K-ras^{V12}$-transformed cells. Ethanol treatment inhibited the cell proliferation and the DNA synthesis of control parental fibroblasts in a time- and dose-dependent manner. In contrast, ethanol did not suppress the proliferation of either oncogenic $H-ras^{V12}$ - or $v-K-ras^{V12}$ -transformed fibroblasts. Microinjection of oncogenic $H-Ras^{V12}$ protein induces DNA synthesis and ethanol treatment did not interfere with the DNA synthesis. The antiproliferative toxicity of ethanol was rescued by antioxidants, such as N-acetylcysteine and 4-methlpyrazole. These results indicate that the antiproliferative action site of ethanol toxicity lies upstream or is independent of Ras and ethanol exerts its toxicity through a free radical formation.

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Effective Components on the Taste of Ordinary Korean Soy Sauce (한국재래식 간장의 맛에 영향을 미치는 성분)

  • 김종규;정영건;양성호
    • Microbiology and Biotechnology Letters
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    • v.13 no.3
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    • pp.285-287
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    • 1985
  • To investigate effective constituents of the many taste components in ordinary Korean soy sauce, we analyzed free amino acids, organic acids, free sugars and saline as taste components in ordinary Korean soy sauce, and determined sensory score of the ordinary Korean soy sauce taste with 45 persons of the trained pannels. The relationships between original data transformed with variables and sensory score of the ordinary Korean soy sauce were analyzed by stepwise multiple regression analysis. Eighty five percents of the ordinary Korean soy sauce taste is affected by twenty one kinds (Isoleucine, Leucine, Valine, NaCl, Lactic acid, Alanine, Phenylalanine, Tartaric acid, Sugar(\ulcorner), Proline, Malic acid, Glycine, Tryptophan, Arginine, Glutaric acid, Maltose, Histidine, Glucose, Fructose and Serine) of the taste components by stepwise multiple regression analysis of original data. Eighty one percents of the ordinary Korean soy sance taste is affected by sixteen kinds (Lactic acid, NaCl, Fumaric.Succinic acid, Tyrosine, Tartaric acid, Glycine, Malonic acid, Malic acid, Tryptophan, Glutaric acid, Methionine, Histidine, Cysteine, Maltose, Fructose and (Glutamic acid) of the taste components by stepwise multiple frgression analysis of original data transformed with square root. Eighty five percents of the ordinary Korean soy sauce taste is affected by nineteen kinds (Fumaric.Succinic acid, Lactic acid, Phenylalanine, NaCl, Tyrosine, Sugar(\ulcorner), Tartaric acid, Leucine, Glutaric acid, Methionine, Glycine, Tryptophan, Histidine, Proline, Cysteine, Glutamic acid, Maltose, Threonine and Oxalic acid) of the taste components by stepwise multiple regression analysis of original data transformed with logarithm.

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Using Web2.0 Techniques to Overcome the Limitations of Current DACUM Method : A Case Study (Web2.0을 이용한 DACUM 기법의 변형과 활용 : 서울시 교육체계 수립 사례를 중심으로)

  • Kim, Min-Jeong;Hwang, Eun-Dong
    • Journal of Information Technology Services
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    • v.7 no.3
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    • pp.159-174
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    • 2008
  • The purpose of this study was to introduce a case that used web2.0 techniques to overcome the limitations of current DACUM method. For the purpose, firstly, we summarized the characteristics and limitations of current DACUM as a method to develop an occupational curriculum. The summary indicated that the results of DACUM process are likely to be influenced by the selection procedure of out-performers. In addition, current DACUM method is difficult to be adapted to big organizations, difficult to reflect rapidly changing society, and lack of qualitative review for the findings. To overcome these limitations, Seoul city designed a transformed DACUM method based on web2.0 techniques. The most outstanding features of the transformed DACUM method were web-based peer review systems to validate the findings of DACUM workshop. We concluded that the transformed DACUM method was proper to big size organizations faced with rapid changes and some organizations with special needs to establish their training systems based on the results of job analysis. Finally, we discussed several practical tips for the Seoul city's method.

Biometric Image Cryptographic Algorithm Based on the Property of Wavelet Transform Coefficient (웨이브렛 변환 계수의 특성을 이용한 생체 영상 암호화 알고리즘)

  • Shin, Jonghong
    • Journal of Korea Society of Digital Industry and Information Management
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    • v.12 no.2
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    • pp.41-49
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    • 2016
  • Lossless encryption methods are more applicable than lossy encryption methods when marginal distortion is not tolerable. In this research, the author propose a novel lossless symmetric key encryption/decryption technique. In the proposed algorithm, the image is transformed into the frequency domain using the lifting wavelet transform, then the image sub-bands are encrypted in a such way that guarantees a secure, reliable, and an unbreakable form. The encryption involves scattering the distinguishable frequency data in the image using a reversible weighting factor amongst the rest of the frequencies. The algorithm is designed to shuffle and reverse the sign of each frequency in the transformed image before the image frequencies are transformed back to the pixel domain. The results show a total deviation in pixel values between the original and encrypted image. The decryption algorithm reverses the encryption process and restores the image to its original form. The proposed algorithm is evaluated using standard security and statistical methods; results show that the proposed work is resistant to most known attacks and more secure than other algorithms in the cryptography domain.

Production of Human Serum Albumin in Chloroplast-Transformed Tobacco Plants

  • Ko, Suk-Min;Kim, Hyun-Chul;Yoo, Byung-Ho;Woo, Je-Wook;Chung, Hwa-Jee;Choi, Dong-Woog;Liu, Jang-R.
    • Journal of Plant Biotechnology
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    • v.33 no.4
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    • pp.233-236
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    • 2006
  • Human serum albumin (HSA) is the most abundant protein in plasma and is the most often used intravenous protein in many human therapies. However, HSA is currently extracted only from plasma because commercially feasible recombinant expression systems are not available. This study attempted to develop an efficient system for recombinant HSA production by chloroplast transformation of tobacco. A HSA cDNA was isolated from a cDNA library constructed with human liver tissue. Chloroplast transformation vectors were constructed by introducing various regulatory elements to HSA regulatory sequences. Vectors were delivered by particle bombardment into leaf explants and chloroplast-transformed plants were subsequently regenerated into whole plants. Southern blot analysis confirmed that the HSA cDNA was incorporated between rps12 and orf70B of the chloroplast genome as designed. Western blot analysis revealed that hyper-expression and increasing the stability of HSA were achieved by modification of the regulatory sequences using the psbA5'UTRs in combination with elements of the 14 N-terminal amino acids of the GFP and the FLAG tag. However, only plants transformed with the vector containing all of these elements were able to accumulate HSA.

Disease Occurrence in Transgenic Rice Plant Transformed with Silbene Synthase Gene and Evaluation of Possible Horizontal Gene Transfer to Plant Pathogens

  • Yu, Sang-Mi;Jeong, Ui-Seon;Lee, Ha Kyung;Baek, So Hyeon;Kwon, Soon Jong;Lee, Yong Hoon
    • Research in Plant Disease
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    • v.20 no.3
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    • pp.189-195
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    • 2014
  • Genetic engineering is being used to enhance disease resistance and nutritional value of crops including rice plant. Considering the fast-growing agricultural biotechnology and rapidly increasing global area of transgenic crops, the risk evaluation on environment is necessary. In this study, we surveyed the difference of disease occurrence between transgenic rice variety, Iksan526 transformed with peanut stilbene synthase gene and non-transgenic rice varieties, Dongjin and Nampyeong in the field. Moreover, the possibility of gene transfer from transgenic rice to bacterial and fungal pathogens was investigated. The results of this study indicated that there was no significant difference in the occurrence and severity of the diseases between Iksan526 and Dongjin or Nampyeong. In addition, the results suggested that rice pathogen, such as Xanthomonas oryzae pv. oryzae, Rhizoctonia solani and Magnaporthe grisea did not take up stilbene synthase and bar genes under natural conditions. Moreover the transformed DNA was not transferred to the pathogens even in repetitive contacts.