• Title/Summary/Keyword: transformation group

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Transformation of PAT gene into Lettuce (Lactuca sativa L.) using Agrobacterium tumefaciens (Agrobacterium tumefaciens를 이용한 상추 (Lactuca sativa L.)의 PAT유전자 형질전환)

  • 류정아;김창길;이현숙;최경배;양덕춘
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.4
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    • pp.197-200
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    • 2001
  • Agrobacterium tumefaciens MP90 harboring PAT (phosphinothricin acetyltransferase) and NPTII-GUS gene were used for the genetic transformation of lettuce (Lactuca Sativa L.). Shoot regeneration from cotyledon explants were obtained from the MS medium supplemented with 0.1 mg.L$^{-1}$ NAA, 1.0 mg.L$^{-1}$ 2ip, 50 mg.L$^{-1}$ kanamycin and 500 mg.L$^{-1}$ carbenicillin after cocultivation with A. tumefaciens for 2 days. Kanamycin resistance test of transgenic plants indicated that the NPTII gene was integrated into the lettuce genome and was stably expressed. PCR and northern blot analysis indicated that bialaphos resistance gene (PAT) was stably integrated into the lettuce genome. The transgenic plant sprayed with Basta (1500x) remained healthy with continuous growth, while the control group exhibited fatality.

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Dynamics of Democratic Labor Union Movement since 1987 in South Korea (1987년 이후 민주노조운동의 동학)

  • Cho, Hyorae
    • Korean Journal of Labor Studies
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    • v.24 no.1
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    • pp.29-64
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    • 2018
  • This paper reviews the dynamics of growth and decline of Democratic Labor Union(Minju-nojo) Movement from the perspective of the conflicts among the industrial relations actors on the institutional incorporation of KCTU, a transformation of industrial relations institutes, and labor flexecurity in the change of political opportunity. The negotiations for the institutional incorporation of KCTU concluded in the 1997-98 labor laws. Since 1998, the conflicts and the compromises on the change of 1997-98 labor laws has continued. It was a kind of games for the transformation of the institutions of industrial relations and labor flexcurity. But the 1997-98 labor laws has shown the path-dependency. Since 2008, a cycle of the movement has entered a decline stage. The decline of Democratic Labor Union Movement came from the institutionalization of the movement, the change of political opportunity, and a generational change of 1987 cohorts group which has been the center of the movement.

A Verification on the Statistical Significance between Groups Using Regression Analysis (회귀분석을 이용한 집단 간 통계적 유의성 검증에 관한 연구)

  • Nam, Soo-Tai;Shin, Seong-Yoon;Jin, Chan-Yong
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2019.05a
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    • pp.163-164
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    • 2019
  • In this study, we compared the differences between groups in a model that investigates the effect of smartphone users on the intent to use continuously. There are various methodologies for group difference analysis, but in this study, we try to verify the size comparison of regression analysis $R^2$. In order to analyze the difference between groups, we try to prove through hypothesis test whether there is a meaningful difference in the intention of continuous use of Korean and Chinese smartphone users collected through previous research. The results of the analysis are useful as a method to determine whether smartphone users in China and Korea are aware of differences or not. According to this procedure, first, the formula for calculating Z-transformation of Fisher and Z-score test statistic calculation formula were used. However, this methodology is also used in the verification of control effect using correlation coefficient. Also, the theoretical implications are presented based on the analysis results.

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Variation of Li Diffusion Coefficient during Delithiation of Spinel LiNi0.5Mn1.5O4

  • Rahim, Ahmad Syahmi Abdul;Kufian, Mohd Zieauddin;Arof, Abdul Kariem Mohd;Osman, Zurina
    • Journal of Electrochemical Science and Technology
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    • v.13 no.1
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    • pp.128-137
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    • 2022
  • For this study, the sol gel method was used to synthesize the spinel LiNi0.5Mn1.5O4 (LNMO) electrode material. Structural, morphological, electrochemical, and kinetic aspects of the LNMO have been characterized. The synthesized LNMO was indexed with the Fd3m cubic space group. The excellent capacity retention indicates that the spinel framework of LNMO has the ability to withstand high rate charge-discharge throughout long cycle tests. The Li diffusion coefficient (DLi) changes non-monotonically across three orders of magnitude, from 10-9 to 10-12 cm2 s-1 determined from GITT method. The variation of DLi seemed to be related to three oxidation reactions that happened throughout the charging process. A small dip in DLi at the beginning stage of Li deintercalation is correlated with the oxidation of Mn3+ to Mn4+. While two pronounced DLi minima at 4.7 V and 4.75 V are due to the oxidation of Ni2+/Ni3+ and Ni3+/Ni4+ respectively. The depletion of DLi at the high voltage region is attributed to the occurrence of two successive phase transformation phenomena.

Effects of the Soybean Powder with Rich Aglycone Isoflavone on Lipid Metabolism and Antioxidative Activities in Hyperlipidemic Rats (고지혈증 흰쥐에서 비배당체 이소플라본 고함유 대두분말의 혈청 지질 대사 영향과 항산화효과)

  • Lim, Ae-Kyoung;Jung, Hee-Kyoung;Hong, Joo-Heon;Oh, Jung-Suk;Kwak, Jung-Hoon;Kim, Yong-Hae;Kim, Dae-Ik
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.3
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    • pp.302-308
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    • 2008
  • This study was performed to evaluate the effects of soybean powder with rich aglycone isoflavone through bio-transformation on lipid metabolism and antioxidative activites in diet induced hyperlipidemic rats. The rats were randomly divided into four groups: NO (basal diet normal group), CO (high fat diet control group), BFF10 (soybean powder with rich aglycone isoflavone 10% group), and BFF20 (soybean powder with rich aglycone isoflavone 20% group). After 7 weeks of BFF10 or BFF20 diets consumption, the concentrations in serum triglyceride, total cholesterol, LDL-cholesterol, and atherogenic index ratios were significantly decreased in the BFF10 and BFF20 diets groups compared with those in the CO group. The activities of alanine amino transferase and aspartate amino transferase were significantly decreased in the BFF10 and BFF20 than those in the CO group. Thiobarbituric acid reactive substance levels of serum were decreased in BFF10 and BFF20 groups compared to that of the CO group. The super oxide dismutase activites were increased in BFF10 and BFF20 groups compared to that of the CO group.

Isolation and characterization of a novel DNA segment that enables the plasmids to replicate autonomously in Aspergillus nidulans (Aspergillus nidulans에서 플라스미드의 자가복제를 유발하는 DNA절편의 분리 및 분석)

  • Kim, Jin-Hee;Han, Kyu-Yong;Han, Kap-Hoon;Han, Dong-Min
    • Korean Journal of Microbiology
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    • v.34 no.3
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    • pp.120-125
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    • 1998
  • A plasmid pNPG contains a genomic DNA complementing npgA1 which is located on the left arm of linkage group I. It transformed Aspergillus nidulans at a high frequency. No abortive transformants were observed and the $Trp^+$ transformants were all $Npg^+$. The 10.4 kb Psti fragment of the genomic DNA was subcloned into pILJ16, which increased the transformation efficiency by more than 200-folds. The transformants were mitotically unstable and yielded $Arg^-$ conidia at the frequency of more than 80%. An additional gene cloned into the plasmid containing the fragment was always lost with $argB^+$ marker. These characteristics strongly indicate the possibility that the plasmids autonomously replicate. The full activity of enhanced transformation was retained on the 4.9 kb EcoRI-HaeIII fragment. The DNA segment was similar to AMA1 rather than ANS1 in function and designated AMA2.

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Bitcoin Distribution in the Age of Digital Transformation: Dual-path Approach

  • Lee, Won-Jun;Hong, Seong-Tae;Min, Taeki
    • Journal of Distribution Science
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    • v.16 no.12
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    • pp.47-56
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    • 2018
  • Purpose - The potential use of cryptocurrencies in a retail environment proposes a rapid shift from the traditional financial system. Nakamoto(2008) defines Bitcoin as an open source alt-coin based on the blockchain technology. Luther(2016) insists that the new technology will be widely adopted for the digital payment processes. However, the use of Bitcoin is in the real world is still sparse. Despite the growing attention and purported benefits, it is doubtful whether the Bitcoin will be eagerly accepted by ordinary consumers in the mainstream market. To answer this question, this paper develops a causal model that has a dual path to explain the motivation to adopt Bitcoin. According to Glaser, Zimmermann, Haferkorn, Weber, and Siering(2014), Bitcoin is both an asset and a currency at the same time. In summary, the attitude towards Bitcoin may vary depending on whether the fin-tech product is viewed as an asset or as a currency. Based on the arguments, we propose that asset attitude and currency attitude will give influence to consumers' intention to adopt Bitcoin. Research design, data, and methodology - Quantitative data collection is conducted from a Bitcoin SIG(special interest group) working in an internet community. As a result, 192 respondents who know Bitcoin completed the survey. To analyze the causal relations in the research model, PLS-SEM(partial least squares structural equation modeling) method is used. Also, reliability and validity of measures are tested by performing Cronbach's alpha test, Fornell-Larcker test and confirmatory factor test. Results - Our test results show that every hypothesis is supported except the influence of perceived ease of use. In addition, we find that the relationships between constructs are different between the high innovative group and low innovative group. Conclusions - We provide evidence that asset attitude and currency attitude are key antecedents of Bitcoin adoption.

The effect of resin cement type and cleaning method on the shear bond strength of resin cements for recementing restorations

  • Koodaryan, Roodabeh;Hafezeqoran, Ali;Maleki, Amin Khakpour
    • The Journal of Advanced Prosthodontics
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    • v.9 no.2
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    • pp.110-117
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    • 2017
  • PURPOSE. This laboratory study assessed the effect of different dentin cleaning procedures on shear bond strength of resin cements for recementing prosthesis. MATERIALS AND METHODS. A $4{\times}4$ flat surface was prepared on the labial surface of 52 maxillary central incisors. Metal frames ($4{\times}4{\times}1.5mm$) were cast with nickel-chromium alloy. All specimens were randomly divided into 2 groups to be cemented with either Panavia F2.0 (P) or RelyX Ultimate (U) cement. The initial shear bond strength was recorded by Universal Testing Machine at a crosshead speed of 0.5 mm/min. Debonded specimens were randomly allocated into 2 subgroups (n = 13) according to the dentin cleaning procedures for recementation. The residual cement on bonded dentin surfaces was eliminated with either pumice slurry (p) or tungsten carbide bur (c). The restorations were rebonded with the same cement and were subjected to shear test. Data failed the normality test (P < .05), thus were analyzed with Mann Whitney U-test, Wilcoxon signed rank test, and two-way ANOVA after logarithmic transformation (${\alpha}=.05$). RESULTS. The initial shear bond strength of group P was significantly higher than group U (P = .001). Pc and Uc groups presented higher bond strength after recementation compared to the initial bond strength. However, it was significant only in Pc group (P = .034). CONCLUSION. The specimens recemented with Panavia F2.0 provided higher bond strength than RelyX Ultimate cement. Moreover, a tungsten carbide bur was a more efficient method in removing the residual resin cement and increased the bond strength of Panavia F2.0 cement after recementation.

Curie Temperature and Tunable Dielectric Properties of Barium Strontium Titanate Thick Films (티탄산 바륨 스트론튬 (BaxSr1-xTiO3) 후막의 상전이온도와 가변 유전특성)

  • Jeon, So-Hyun;Kim, In-Sung;Min, Bok-Ki;Song, Jae-Sung;Yoon, Jon-Do
    • Journal of the Korean Ceramic Society
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    • v.43 no.7 s.290
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    • pp.421-426
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    • 2006
  • [ $(BaSr)TiO_3$ ] thick films were prepared by tape casting method, using $BaTiO_3\;and\;SrTiO_3$ powder slurry in order to investigate dielectric properties i.e. dielectric constant, ${\varepsilon}_r$, Curie temperature, $T_c$. Grain growth within $(BaSr)TiO_3$ thick films was observed with increasing weight ratio of $BaTiO_3$. This observation can be explained by phenomena of substitution of $Sr^{2+}$ ion for $Bi^{2+}$ ion in the $BaTiO_3$ system. Also, the Curie temperature in $(BaSr)TiO_3$ thick films was shifted to lower temperature range with increasing $ SrTiO_3$. Furthermore, Curie temperature having maximum dielectric constant was in the range of $-40^{\circ}C\;to\;30^{\circ}C$, and hence sharper phase transformation occurred at Curie temperature. There occurred decrease in tunability and k-factor of $(Ba_{0.6}Sr_{0.4})TiO_3$ calculated from the dielectric constant, ${\varepsilon}_r$ above Curie temperature. In addition, above the $60^{\circ}C$, phase fixation was observed. This means that internal stress relief occurred with increasing $90^{\circ}$ domains.

Threshold Crossing Rate, Phase Distribution and Group Properties of Nonlinear Random Waves of finite Bandwidth (유한한 Bandwidth를 갖는 비선형 불규칙 파열에서의 Threshold Crossing Rate, 위상분포와 파군특성)

  • Jo, Yong-Jun
    • Journal of Korea Water Resources Association
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    • v.30 no.3
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    • pp.225-233
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    • 1997
  • The nonlinear effects on the statistical properties of wave groups in terms of the average nomber of waves in a group and the mean number of waves in a high run is studied in this paper utilizing the complex envelope and total phase function, random variable transformation technique and perturbation method. It tures out that the phase distribution is modified significantly by nonlinearities and it show systematic excess of values near the mean phase and the corresponding symmetrical deficiency on both sides away from the mean. for the case of threshold crossing rate, it turns out that threshold crossing rate reaches its maxima just below the mean water level rather than zero and considerable amount of probability mass is shifted toward the larger values of water surface elevation as nonlinearity is getting profound. Furthermore, the mean waves in a high run associated with nonlinear wave are shown to have larger values than the linear counterpart. Similar trend can also be found in the average number of waves in a group.

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