• Journal of Microbiology and Biotechnology
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• v.10 no.1
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• pp.91-94
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• 2000

The suspension cells of Taxus cuspidata were transformed with Agrobacterium tumefaciens harboring binary vector pCAMBIE1302 encoding mgfp. Transient transfection efficiency was compared by using the fluoremetric measurement. The transient transfection efficiency was improved by transformation with DMSO and/or sonication treatment. Optimum conditions for DMSO and sonication treatment were 3% and 30sec, respectively. selection and maintenance of transformed cells were continued for 3 months. An insertion of the mgfp gene in transformed cells was detected by PCR and an expression of GFP confirmed by the western blot analysis.

• Proceedings of the KIEE Conference
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• 1999.11c
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• pp.555-557
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• 1999

This paper implements an active noise controller in a headset by solving Robust $H_{\infty}$ control problem. In the $H_{\infty}$ control framework, we can suppose the noise in a headset as disturbance and the noise control problem is cast on the well-known $H_{\infty}$ regulation problem. By representing the system as LFT(Linear Fractional Transformaion) form, the controller is obtained using D-K iteraitons. The designed controller was implemented with operational amplifiers and it produced the desired noise reduction performance.