• Microbiology and Biotechnology Letters
• /
• v.19 no.4
• /
• pp.356-361
• /
• 1991

- To investigate the possibility of genetic development for a multi-purpose strain of Bacillus subtilis YBL-7 against Fusat-iurn solani causing root rot of many impotant corps, the plasmid pGU66 inserting urease gene of Bacillus pasteurii had been introduced into Bacillus subtilis YBL-7 by PEG-induced protoplast (PIP) transformation system. Protoplasts of B. subtilis YBL-7 were prepared by treating the cells with lysozyme (200 $\mu g$/ml) in hypertonic buffer (SMMP). The highest transformation frequency was achieved when cells of the strain with lysozyme at $42^{\circ}C$ for 90 minutes. Optimal transformation was obtained using polyethylene glycol (MW 4000) at final concentration of 30% (V/V). The transformation frequency was increased proportionally to 1.2 $\mu g$ of plasmid DNA. At best condition, the transformation frequency (transformants/ regenerants/$\mu g$ of DNA) for pGU66 was appoximately $4 \times 10^{-3}$. Also, the urease gene was strongly expressed in the transformants of B. subtilis YBL-7 and maintained steadily. The antifungal ability of transformant was very similar to that of B. ssubtilis YBL-7.

• Journal of the Korea Society for Simulation
• /
• v.15 no.3
• /
• pp.79-86
• /
• 2006

This paper proposes a new protocol to enhance the routing performance and applies our proposal to AODV (Ad hoc On-demand Distance Vector routing) protocol, which is a typical on-demand routing protocol in MANET. In general, when a route repair procedure is performed due to the transfer or failure of a node, the source node re-initiate a route discovery procedure in most routing protocols of MANET. This causes to increase the control traffic overhead largely and degrade the routing performance. The main idea of our study is to provide a methodology that can minimize the control traffic overhead and enhance the performance by initiating a route repair procedure at destination node and utilizing the previous routing information maximumly. To evaluate the performance of the our mechanism, we have performed a series of simulations. The simulations include comparisons with AODV and AODV with local repair. The simulations have been executed under simulation environments taking into account mobility speed and network site. The simulation results show that our proposal overall outperforms the existing protocols.

• KSBB Journal
• /
• v.25 no.2
• /
• pp.167-172
• /
• 2010

Streptomyces scabiei producing phytotoxin called thaxtomin, which cause scab disease on economically important crops such as potato. For molecular genetics study of S. scabiei an effective transformation method was established based on conjugal transfer from Escherichia coli ET12567 (pUZ8002) using a phiC31-derived integration vector, pSET152, containing oriT and attP fragments. The high frequency was obtained on MS medium containing 50 mM $MgCl_2$. In addition, the sequence and location of the chromosomal integration attB site of S. scabiei was identified for the first time in the strains producing thaxtomin by the southern blot analysis of exconjugants and the sequencing of plasmid containing DNA flanking the insertion sites from exconjugant chromosome. Similar to the case of Streptomyces species, a single phiC31 attB site of S. scabiei is present within an ORF encoding a pirin-homolog.

• Journal of Korean Association for Spatial Structures
• /
• v.8 no.4
• /
• pp.73-80
• /
• 2008

The objective of current study is to develop an optimization technique for the seismic actively controlled building structures using active tendon devices by an efficient solution of LQR control gain. In order to solve the active control system, the Ricatti closed-loop algorithm has been applied, and the state vector has been formulated by the transfer matrix and solved by a numerical technique of the trapezoidal rule. The time-delay problem has been also considered by phase compensation. To optimize the performance index, the ratio of the weighted matrix is the design variable, allowable story drift limits of IBC 2000 and tendon forces have been applied as restraint conditions, and the optimum control program has been developed with the algorithm of the SUMT technique. In examples of the optimization problem of eight stories shear buildings, it is evaluated that the optimum controlled building is more suitable in the control of earthquake response than the uncontrolled system and can reduce the performance index to compare with the controlled system with a constant ratio of the weighted matrix.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2005.03a
• /
• pp.354-361
• /
• 2005

Physical parameters such as porosity and effective porosity are important physical parameters that determine the transfer and movement of water and solutes in porous media. Various methods of determining these parameters have been developed, with varying degrees of accuracy and applicability. Most of the existing methods produce static results. They do not produce instantaneous and real time of porosity and effective porosity in a porous media. In this study, a new permittivity method called Frequency Domain Reflectometry with Vector analyzer (FDR-V) is proposed to determine the porosity and effective porosity of some sand samples in the laboratory. The advantage of the FDR-V method is that it instantaneously determines the temporal variation of dielectric constants of porous media. Then, the porosity and the effective porosity of porous media are computed using well established empirical equations. Results obtained from the FDR-V method compared favorably with results from other permittivity methods such as gravimetric, injection and replacement tests. The ratio of effective porosity to porosity was $85{\sim}92%$, when FDR-V was used. This value compared favourably with 90%, which has been usually quoted in previous studies. Considering the convenience and its applicability, the measurement system of FDR-V permittivity holds a great potential in porous media and contaminant transport studies.

• Journal of Plant Biotechnology
• /
• v.30 no.2
• /
• pp.115-121
• /
• 2003

In this study, plant regeneration and Agrobacterium tumefaciens-mediated transformation Kentucky bluegrass(Poa pratensis L.) were evaluated. Three different types of calli were produced depending on the combinations of growth regulators. They were non-friable brown or gray-colored callus (type I), compact, friable and yellow or white-colored callus (typeII), and soft, watery translucent callus with differentiated structure (typeIII). The highest regenerable organogenic callus (typeII) was obtained on the medium containing 1mg/L, 2,4-D and 0.1mg/L BA. Additionally, the production of typeII calli increased significantly when AgNO$_3$ was added to the callus induction and growth medium. The highest frequency of multiple shout formation from typeII callus was obtained on MS medium containing 1mg/L BA and 1mg/L Thidiazuron(TDZ). The organogenic calli(typeII) were inoculated with Agrobacterium tumefaciens strain EHA101 harboring the binary vector pIG121Hm with $\beta$-glucuronidase gene, and various factors were found to influence the transfer-DNA delivery efficiency. The highest transient GUS activity was observed on typeIIcallus. In the present work, we reported the first transient GUS activity of Kentucky bluegrass mediated by Agrobacterium tumefaciens. Our system may contribute to genetic improvement for breed-recalcirtrant grass species, Kentucky bluegrass.

• Applied Chemistry for Engineering
• /
• v.27 no.3
• /
• pp.335-341
• /
• 2016

In this study, impurity effects on diffusive-convection flow fields by physical vapor transport under terrestrial and microgravity conditions were numerically analyzed for the mixture of $Hg_2Cl_2-I_2$ system. The numerical analysis provides the essence of diffusive-convection flow as well as heat and mass transfer in the vapor phase during the physical vapor transport through velocity vector flow fields, streamlines, temperature, and concentration profiles. The total molar fluxes at the crystal regions were found to be much more sensitive to both the gravitational acceleration and the partial pressure of component $I_2$ as an impurity. Our results showed that the solutal effect tended to stabilize the diffusive-convection flow with increasing the partial pressure of component $I_2$. Under microgravity conditions below $10^{-3}g_0$, the flow fields showed a one-dimensional parabolic flow structure indicating a diffusion-dominant mode. In other words, at the gravitational levels less than $10^{-3}g_0$, the effects of convection would be negligible.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.7 no.2
• /
• pp.127-131
• /
• 2003

Protein disulfide isomerase (PDI) found in the endoplasmic reticulum (ER) catalyzes disulfide bond exchange and assists in protein folding of newly synthesized proteins. PDI also functions as a molecular chaperone and has been found to be associated with proteins in the ER. In addition, PDI functions as a subunit of two more complex enzyme systems: the prolyl-4-hydroxylase and the triacylglycerol transfer proteins. A cDNA that encodes protein disulfide isomerase was previously isolated from Bombyx mori (bPDI), in which open reading frame of 494 amino acids contained two PDI-typical thioredoxin active site of WCGHCK and an ER retention signal of the KDEL motif at its C-terminal, and we report its functional characterization here. This putative bPDI cDNA is expressed in insect Sf9 cells as a recombinant proteins using baculovirus expression vector system. The bPDI recombinant proteins are successfully recognized by antirat PDI antibody, and shown to be biologically active in vitro by mediating the oxidative refolding of reduced and scrambled RNase. This suggests that bPDI may play an important role in protein folding mechanism of insects.

• Aerospace Engineering and Technology
• /
• v.8 no.2
• /
• pp.83-91
• /
• 2009

This paper concerns the dynamic property of TVC system in the upper stage of KSLV-I. The minimum bandwidth of TVC system is predicted by gathering and comparing the dynamic test data through whole development phases of KSLV-I. The linear models which approximate the dynamic data are also suggested. It is shown that the minimum bandwidth of KSLV-I TVC system is guaranteed over 6.0 Hz at one degree command. It is also shown that the linear model of KSLV-I TVC dynamics takes the form of the transfer function with an 8-th order denominator and a 2-nd order numerator. These results will play an important role in analyzing the flight stability and performance of KSLV-I.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 2003.10a
• /
• pp.369-372
• /
• 2003

This paper describes a design of OFDM (Orthogoanl Frequency Division Multiplexing) based wireless LAN system receiver, defined in IEEE 802.11a standards. Because OFDM system uses several orthogonal sequence sets, it ran avoid selective fading of fast data transfer problem when it is used with error correction code. But if the receiver is not synchronized, the orthogonal of between sub-ralliers will be destroyed and the data interruption will be generated. So it makes error property get worse very murk. For improving the noise error, we use the relationship of phasor between sub-carriers and make system synchronization using one tab equalizer. The designed OFDM block is described by Verilog HDL for the efficient and small size hardware. And we preform the functional verification and evaluation using the vector of standards.