• 한국방송∙미디어공학회:학술대회논문집
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• 한국방송공학회 2009년도 IWAIT
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• pp.703-708
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• 2009

The present paper describes the application of an improved impression transfer vector method (Sakurai et al., 2007) to transform the three basic dimensions (Evaluation, Activity, and Potency) of higher-order impression. First, a set of shapes and surface textures of faces was represented by multi-dimensional vectors. Second, the variation among faces was coded in reduced parameters derived by applying principal component analysis. Third, a facial attribute along a given impression dimension was analyzed to select discriminative parameters from among principal components with higher sensitivity to impressions, and obtain an impression transfer vector. Finally, the parametric coordinates were changed by adding or subtracting the impression transfer vector and the image was manipulated so that its facial appearance clearly exhibits the transformed impression. A psychological rating experiment confirmed that the impression transfer vector modulated three dimensions of higher-order impression. We discussed the versatility of the impression transfer vector method.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2013년도 춘계학술대회
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• pp.1006-1008
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• 2013

A novel recombinant baculovirus vector system containing coding genes for polyhedron promoter, vesicular stomatitis virus G (VSVG), polyA, cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP), and protein transduction domain (PTD) was constructed. We applied this recombinant baculovirus vector into cells and murine tissues and compared efficacy of gene transfer and expression of this recombinant baculovirus vector system with control vector system. From this result, we confirmed that this novel recombinant baculovirus vector system was very effective than control vector system.

• 한국잠사곤충학회지
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• 제37권1호
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• pp.46-51
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• 1995

국내 분리주의 BmNPV를 이용하여 유용 단백질을 생산할 수 있는 새로운 전이벡터의 제작과 외내 유전자의 발현에 대한 연구결과는 다음과 같다. 1. PCR 기법에 의하여 다각체 단백질 유전자의 ＋1 - －194에 해당하는 promoter 부위를 증폭하고 클로닝 하였다. 2. 다각체 단백질 유전子의 5' 및 3'의 leader 부위를 promoter 부위와 함께 단계적으로 클로닝하여 전이벡터를 완성하였다. 3. 완성된 전이벡터는 pBmKSK1으로 명명하였으며, 염기서열 결정을 통하여 다각체 단백질 유전자의 ＋2 부터 ＋597까지 제거되고 외내 유전자의 클로닝 sites로 EcoRI, KpnI과 SacI을 가짐을 확인하였다. 4. 외래 유전자로서 E. coli의 $\beta$-galactosidase 유전자를 pBmKSKl에 클로닝하고 누에 세포주에 전이시킨 후, X-gal 염색 및 SDS-PAGE에 의하여 pB- mKSKl이 제 기능을 수행함을 확인하였다.

• Journal of Microbiology and Biotechnology
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• 제9권5호
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• pp.541-547
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• 1999

Constructions of a transfer vector and a recombinant baculovirus using the thymidine kinase gene of the Herpes simplex virus type 1 strain F (HSV -1) were carried out. Newly cloned transfer vector, pHcgXIIIB, was constructed by insertion of the glycoprotein gX gene signal peptide sequence of Pseudorabies virus into the baculovirus vector pHcEV-IV. The gX sequence was inserted just downstream from the promoter for the polyhedrin gene of the Hyphantria cunea nuclear polyhedrosis virus (HcNPV). HSV-1 thymidine kinase(tk) gene (1.131 kb) was used as a candidate gene for transferring into the baculovirus expression system. The tk gene was inserted into a BamHI site downstream from the gX sequence-promoter for the polyhedrin gene in the pHcgXIIIB transfer vector and was transferred into the infectious lacZ-HcNPV expression vector. Recombinant virus was isolated and was named gX-TK-HcNPV. The recombinant virus produced a 45 kDa gX-TK fusion protein in Spodoptera frugiperda cells, which was confirmed by Western blot analysis. Microscopic examination of gX-TK-HcNPV-infected cells revealed normal multiplication. Fluorescent antibody staining indicated that the gX-TK fusion protein was present in the cytoplasm. These results indicated that the transfer vector successfully transferred the gX-tk gene into the baculovirus expression system.

• 한국잠사곤충학회지
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• 제39권2호
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• pp.180-185
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• 1997

To develope the novel baculovirus transfer vector, the p10 gene was cloned from the Bombyx mori nuclear polygedrosis virus (BmNPV) vB2 strain isolated from the B. mori larvae of sericultural farms. The novel transfer vector was constructed by using the p10 gene of BmNPV vB2 strain was 210 bp. The TAAG sequence at the -71 bp of upstream from translation initiator ATG and two polyadenylation signal site at the downstream from terminator TAA were also detected in the p10 gene. The 5' and 3' flanking region of the p10 gene amplified by PCR was cloned into pBluescriptII SK(+) and then transfer vector pBm10 was construceted. The 7.9 kb pBm10 was analysed by restriction enzymes and the map was confirmed. In order to determine the expression of foreign gene of pBm10, $\beta$-galactosidase gene was inserted in the SmaI site of foreign gene cloning site of pBm10. The pBm10 containing $\beta$-galactosidase gene was cotranfected wth genomic DNA of BmNPV vB2 into BmN-4 cells. The recombinant baculovirus expressing $\beta$-galactosidase was also produced polygedra in the infected cells. The results indicated that pBm10 is functional, suggesting that in the baculovirus expression vector system, the recombinant virus produced by pBm10 was effective by oral infection for the producing recombinant proteins in in vivo expression.

• 대한전기학회논문지:전력기술부문A
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• 제54권6호
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• pp.274-283
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• 2005

This paper presents a new method to derive energy function based on vector product. Using this method, an energy function to consider transfer conductances and capacitors is derived. Then we recommend a voltage collapse criteria to predict the voltage collapse in power systems by using the energy margin derived by the proposed energy function. This energy function is applied to a 2-bus power system reflecting transfer conductances and capacitors. We show that the energy function derived based on vector product can be applied in order to analyze power system stability and the energy margin can be utilized as a criterion of voltage collapse by simulation for the 2-bus system.

• 한국소음진동공학회논문집
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• 제20권6호
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• pp.562-567
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• 2010

A passenger vehicle has various and complicated transmission paths of sound and vibration. In order to identify the mechanism of transfer path, estimation of excitation force and exact modeling of transfer path are required. In this paper vector synthesis technique is employed to identify the characteristics of road noise and its transmission to vehicle compartment through noise and vibration analysis. Vibration reduction efficiency of each transfer path is evaluated by comparing individual vector components obtained virtual simulation. The degree of effect is used to estimate the contribution of vibration input components to total output. And in this paper presents a new technique based on simulation studies using vector synthesis diagram and design of experiments, by which the effects of magnitude and phase change of input paths can be predicted.

• 한국소음진동공학회논문집
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• 제20권11호
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• pp.1071-1077
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• 2010

The reduction of the vehicle interior noise has been the main interest of noise and vibration harshness(NVH) engineers. A passenger vehicle has various and complicated transmission paths of sound and vibration. In order to identify the mechanism of transfer path, estimation of excitation force and exact modeling of transfer path are required. This paper presents method for estimating the noise source contribution on the road noise of the vehicle in a multiple input system where the input sources may be coherent with each other. And vector synthesis technique is employed to identify the characteristics of road noise and its transmission to vehicle compartment through noise and vibration analysis. Vibration reduction efficiency of each transfer path is evaluated by comparing individual vector components obtained virtual simulation.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2013년도 추계학술대회
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• pp.946-948
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• 2013

베큘로바이러스 시스템이 제조되었는데 이것은 polyhedron promoter, vesicular stomatitis virus G (VSVG), polyA, cytomegalovirus (CMV) promoter, enhanced green fluorescent protein (EGFP), protein transduction domain (PTD) 유전자를 재조합한 것이다. 본 재조합벡터 시스템은 인간 섬유아세포에 적용하여 시험하였고 재조합된 유전자의 전이와 유전자 발현을 대조 벡터시스템과 비교하였다. 본 연구로부터 새롭게 제작된 본 베큘로바이러스 시스템이 유전자의 전이와 유전자 발현 면에서 대조 벡터시스템 보다 고효율을 나타내었다.

• 한국정보통신학회논문지
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• 제18권8호
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• pp.2017-2022
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• 2014

폴리히드론 프로모터, 수포성구내염 바이러스G, 폴리A, 사이토메가바이러스 프로모터, 강화녹색형광단백질, 단백질전달부위 유전자 등을 포함한 새로운 베큘로바이러스 시스템이 제조되었다. 본 재조합벡터 시스템은 인간 섬유아세포에 적용하여 시험하였고 재조합된 유전자의 전달과 유전자 발현을 대조 벡터시스템과 비교하였다. 본 연구로부터 새롭게 제작된 본 베큘로바이러스 시스템이 유전자의 전이와 유전자 발현 면에서 대조 벡터시스템 보다 고효율을 나타내었다.