• Journal of Microbiology
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• v.34 no.4
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• pp.311-315
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• 1996

The retroviral Gag polyprotein directs the assembly of virion particles and plays an important role in some events after entry into a host cell. The Gag polyprotein of a virus mixture is responsible for inducing murine acquired immunodeficiency syndrome (MAIDS) when injected into susceptible strains of mice. In order to identify the host cellular proteins which interact with the MAIDS virus Gag proteins and possibly mediate the function of the Gag proteins, mouse T-cell leukemic cDNA expression library was screened using the yeast GAL4 two hybrid system. Of 11 individual positive clones, the clone Y1 was selected for the study of protein-protein interaction. Its DNA sequence revealed that it was an exact match to the murine SH3 domain-containing protein SH3P8. It is expressed as 2.4 kbp transcripts in testis at higher levels and in various tissues tested at lower levels. Glutathione S-transferase-Y1 fusion protein binds tightly to $Pr60^{def-gag}$ as well as $Pr65^{eco-gag}$.

• Fisheries and Aquatic Sciences
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• v.11 no.3
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• pp.177-181
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• 2008

Glyceraldehyde 3-phosphate dehydrogenase(GAPDH) is a key enzyme for carbohydrate metabolism in most living organisms. Recent reports and our own searches of teleost species in publicly available genomic databases have identified at least two distinct GAPDH genes in a given species. The two GAPDH genes are located on the same chromosome in teleosts, whereas they are located on the different chromosomes in mammals. Thus, we reconstructed a phylogenetic tree to better understand the evolutionary history of the GAPDH genes in the vertebrate lineage. Our phylogenetic analysis revealed unambiguously that the two GAPDH genes of teleosts are phylogenetically closely affiliated to one of the cytosolic GAPDH and spermatogenic GAPDH-S of mammals. This indicates that the two paralogous GAPDH genes shared a common ancestor and subsequently underwent a gene duplication event during early vertebrate evolution. However, GAPDH-S of teleosts showed significant differences in the polypeptide residues and tissue distribution of its mRNA transcripts from that of mammals, implying they have undergone a different history of functionalization.

• Journal of the Korean Society for information Management
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• v.20 no.4 s.50
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• pp.49-66
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• 2003

To understand current state of the digital reference services in Korean academic libraries. a comprehensive examination and analysis was performed. Data was collected from July 8, 2003 to July 22, 2003, through direct site examination and recording the question and answer transcripts. The analysis focused on the provision characteristics and use of the digital reference services. Results revealed interesting findings and related problems. Suggestions for future development are provided.

• Journal of Plant Biotechnology
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• v.42 no.1
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• pp.13-18
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• 2015

In this study we confirmed the stable integration of Arabidopsis AVP1 in the genomes of bottle gourd $T_3$ homozygous lines and its transcription, and additionally evaluated possibility of translocation of the AVP1 mRNA from transgenic bottle gourd rootstocks to wild type watermelon scions. Each AVP1 gene in two bottle gourd T3 lines is abundantly expressed under a field condition. Given the grafting between wild type watermelon scions and AVP1-expressing bottle gourd rootstocks, no translocation of the AVP1 mRNA was detected in leaves, both sexual flowers, and fruits of the scions.

• Proceedings of the Korean Society of Life Science Conference
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• 2006.09a
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• pp.45.2-45.2
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• 2006

• Research in Mathematical Education
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• v.16 no.4
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• pp.265-276
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• 2012

These days, with the emphasis on statistical literacy, the importance of communication is the focus of attention. Communication about statistics is important since it is a way of describing the understanding of concepts and the interpretation of data. However, students usually have trouble with expressing what they understand, especially through writing. In this paper, we examined preservice teachers' difficulties when they wrote about statistical concepts. By comparing preservice teachers' written responses and interview transcripts of the variance concept task, we could find the missing information in their written language compared to their verbal language. From the results, we found that preservice teachers had difficulty in connecting terms contextually and conceptually, presenting various factors of the concepts that they considered, and presenting the problem solving strategies that they used.

• Journal of Life Science
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• v.10 no.6
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• pp.610-616
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• 2000

The DPB11 gene, which genetically interacts with DNA polymerase II ($\varepsilon$) encoded by POL2 and DPB2, is required for DNA replication and the S phase checkpoint in Saccharomyces cerevusiae. The transcripts of DPB11 did not fluctuated as evidently as DPB2 and POL2 genes during cell cycle. To identify the physical interaction between Dpb2 and Dpb11, we examined the interaction by two-hybrid system. The interaction between Dpb2 and Dpb11 was detected in a two-hybrid assay. These results suggest that the amount of the Dpb2-Dpb11 complex may peak at the G1/S boundary. Therefore, we strongly suggest the involvement of the Dpb2-Dpb11 complex in a progression of DNA replication and S-phase checkpoint.

• Journal of Applied Biological Chemistry
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• v.42 no.2
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• pp.62-65
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• 1999

Iron regulatory proteins (IRPs) 1 and 2 bind with equally high affinity to specific RNA stem-loop sequences known as iron-responsive elements (IRE) which mediate the post-transcriptional regulation of many genes of iron metabolism. To study putative IRE-like sequences in RNA transcripts using the IRP-IRE interaction, Eight known genes from database were selected and the RNA binding activity of IRE-like sequences were compared to IRP-2. Among them, the IRE-like sequence in 3'-untranslational region (UTR) of divalent ration transporter-1 (DCT-1) shows a significant RNA binding affinity. This finding predicts that IRE consensus sequence present within 3'-UTR of DCT-1 might confer the regulation by IRP-2.

• Journal of The Korean Association For Science Education
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• v.24 no.1
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• pp.121-128
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• 2004

This study was to investigate high students' conceptions of acids and bases, and their views on learning science. Multiple sources of data were collected over six months with a participation of sit tenth graders and their science teacher. The transcripts of interviews and other data were examined with an eye toward students' conceptions of acids and bases, and their views of learning science. Students' views of science are displayed the representative pattern. Each pattern is represented with an episode. Students' views of learning have been found to reflect the transmissive models of science educational practice. Students accept passive and difficult-to-modify views of the learner roles that they should play in the science classroom. Students identified science classes as conservative places, despite the introduction of science literacy as a goal of Korean science education since 1980. Behaviorism remains the major influence in their expectation, design, and practice in school science. Moreover, 'transmission' remains the persistent and dominant classroom cultural dynamic for both teaching and learning of science.

• Child Health Nursing Research
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• v.23 no.2
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• pp.219-228
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• 2017

Purpose: The purpose of this study was to understand and explore the essences of Korean mothers' experiences of caring for visually impaired children. Methods: Data were collected through individual in-depth interviews using open-ended questions. Five mothers of children with visual impairments participated in the interviews. Verbatim transcripts were analyzed using phenomenological analysis. Results: Analysis with Colaizzi's method showed two categories which included 14 themes in 4 theme clusters. The first category was 'surviving in the dark reality' included 2 theme clusters, of 'outcrying in the darkness' and 'enduring the darkness while caring for my child alone'. The second category was 'living as the light of maternal affection' including 2 theme clusters of 'preparing the light to shine in the darkness' and 'going through the darkness with the lights of love'. Conclusion: The findings from this study provide deep understanding of mothers' experiences of caring for visually impaired children. Based on the results of the study, health professionals can develop effective family nursing interventions to improve the quality of life for these families including the visually impaired children and their mothers.