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Plant Disease Caused by Cucumber Mosaic Cucumovirus - Potential Role of Genes Associated with Symptom - (Cucumber Mosaic Cucumovirus에 의한 식물의 병 - 병징관련 유전자의 기능을 중심으로 -)

  • 최장경;김혜자
    • Plant Disease and Agriculture
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    • v.5 no.1
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    • pp.14-19
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    • 1999
  • Cucumber mosaic cucumovirus (CMV) is an isometric plant virus with functionally divided genomic RNAs and a broad host range. RNA 1 and RNA 2 each encode one protein, both of which are essential for replication. RNA 3 encodes the viral coat protein and an additional protein thought to be involved in potentiating the cell-to-cell movement of the virus. Functions of the RNAs have been confirmed using a pseudorecombinant virus constructed with infectious cDNA-derived transcripts of the RNAs. Generally, CMV produces different symptoms in various host plants depending on the virus strains. In this mini-review, we describe the potential role of the genes associated with symptom expression of CMV RNAs.

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Molecular Cloning of a cDNA Encoding Novel Tomato ACC Oxidase Using RT-PCR

  • Yang, Suk-Jin;Hahn, Kyu-Woong
    • Journal of Life Science
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    • v.9 no.2
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    • pp.72-75
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    • 1999
  • Using RT-PCR, a cDNA encoding tomato (Lycopersicon esculentum) ACC oxidase has partially been cloned, sequenced and identified. The nucleotide suquence of the clone was in the coding region and shared about 80% of homology iwht the other ACC oxidase genes of tomato, and 70∼84% with those of other plants such as Oryza sativa, Nicotiana tabacum and Helianthus annuus. In the wounded tomato leaves, this nucleotide transcripts were accumulated rapidly and declined slowly thereafter. These results suggested that the predicted clone might be another member of tomato ACC oxidase gene family.

Perception of Cervical Cancer Screening Among Japanese University Students Who Have Never Had a Pap Smear: A Qualitative Study

  • Oshima, Sumiko;Maezawa, Masaji
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.7
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    • pp.4313-4318
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    • 2013
  • This study aimed to explore attitudes towards cervical cancer screening among Japanese university students who had never had a Pap smear. Four focus-group discussions, each with 15 female university students, took place in November and December 2009. Discussions were recorded and transcripts were analyzed to extract attitudes of young women towards cervical cancer screening. The four themes that emerged were: i) a low sense of reality about cervical cancer; ii) a lack of knowledge about both cervical cancer and Pap smears; iii) a lack of motivation to get screened, and iv) a reluctance to visit the gynecologist. Participants who were interested in undergoing screening for cervical cancer cited the influence of conversations with friends and family, a diagnosis of cancer within their family, and relevant information from the media. The results indicate the importance of getting young women more interested in cervical cancer screening and overcoming their tendency to avoid visiting a gynecologist.

An Exploration on Formative Evaluation Methods for Free Semester System in Middle School (중학교 자유학기제 형성평가 방안 탐색)

  • WON, Hyo-Heon
    • Journal of Fisheries and Marine Sciences Education
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    • v.28 no.1
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    • pp.289-299
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    • 2016
  • The purpose of the study was to develop evaluation methods which would measure student achievement and progress without traditional paper-pencil tests such as mid-term and final examinations. More specifically, the main research focus were to establish general directions of student assessment during free semester, to build evaluation models supporting student's participation and learning, and to report and record various student evaluation results. As research results, we found that student evaluation of free semester should be organized to improve a) experience learning activity, self-regulatory and collaboratory study, b) high-order thinking ability and character-building, and c) teacher-student-parent cooperation. Since traditional paper-pencil tests were restricted in free semester, student achievement should be provided by a way of performance descriptions on transcripts rather than quantitative grade points. Student performance descriptions had to show not only subject knowledge but also students efforts, motivation, and participation. These multiple and educationally meaningful information would be collected by teacher-student-parent communication and collaboration.

Transformation of Lettuce (Lactuce sativa L.) Using Iron Storage Protein Ferritin Gene (철 저장단백질 관련 Ferritin 유전자를 이용한 상추의 형질전환)

  • 김성하;노일섭;최장선;강권규
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.3
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    • pp.147-151
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    • 2001
  • Explants of Lactuce sativa cultivar, chungchima, were co-cultivated with Agrobacterium tumefaciences LBA4404, EHA101 strains containing nptll gene and ferritin gene encoding iron storage protein from soybean for transformation. Through initial selection of regenerated explants by culturing on a kanamycin and carbenicillin containing MS medium, multiple shoots were obtained after 2 months of culture. For a complementary step of selection, putative transgenic shoots were transferred to 1/2 MS basal medium supplemented with 100 mg/L kanamycin and 500 mg/L carbenicillin. The selected shoots were tested with PCR analysis using nptll, ferritin specific primers whether ferritin gene was introduced to genome of the plants. These results confirmed that produced the specific PCR bands in the putative transgenic lines. Additionally the Northern blot showed that transcripts of ferritin gene were detected in mature leaf of the transgenic lines. These results suggest that ferritin gene be successfully integrated and transcribed in the putative transgenic lettuce plants.

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Concept Mapping Analysis of Customers' Cafe Experience (Concept Mapping을 이용한 커피전문점 고객의 경험 인식 분석)

  • Shin, Seo-Young;Cha, Sung-Mi;Chung, Ji-Yoon
    • The Korean Journal of Food And Nutrition
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    • v.23 no.1
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    • pp.94-101
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    • 2010
  • Concept mapping was used to understand the structure of customer's cafe experience. In January and February, 2009, interviews were conducted with 26 subjects residing in the Seoul-Gyeonggi area. One hundred-two statements describing cafe experience were extracted from the interview transcripts, and were sorted and rated for their importance. Concept mapping software was used to generate a concept map denoting seven dimensions of cafe experience('comfort', 'place of my own', 'pleasant atmosphere', 'personal coffee preference', 'service', 'uniqueness', and 'value'). The results provided meaningful dimensions of cafe customers' experience, which ought to be considered when planning a cafe.

The Specificity of Environmental Influence - Home Environment Affects Korean-Chinese Children's Early Language Development via Maternal Speech - (초기 언어발달에 있어 환경적 영향의 특수성 - 중국 조선족 아동의 가정환경에 따른 단어발달에서 어머니 언어의 매개효과 -)

  • Jeon, Hyo-Jeong;Lee, Kwee-Ock;Park, Hyewon
    • Korean Journal of Child Studies
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    • v.25 no.5
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    • pp.163-178
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    • 2004
  • The hypothesis was tested that children whose families differ in socioeconomic status(SES) and educational level differ in their rates of productive language development because they have different language-learning experiences. Naturalistic interaction between mothers and their children was video taped. Transcripts of these interactions provided the basis for estimating the growth in children's productive vocabularies and properties of maternal speech. The sixty children from age 1 to 3 were selected in Yanji, China. The results show that the high educated mothers' children grew more than the low educated mothers' children in their mean length of utterances. Properties of maternal speech that differed as a function of mother's educational level fully accounted for this difference. Implications of these findings for mechanisms of environmental influence on child development are discussed.

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Patterns of Teacher Questioning Discourse in Korean Science Classrooms

  • Shin, Myeong-Kyeong;Yager, Robert E.;Oh, Puil-Seok
    • Journal of the Korean earth science society
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    • v.24 no.2
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    • pp.61-73
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    • 2003
  • This is a descriptive study to identify patterns of teacher questioning discourse. Transcripts from Korean secondary science classrooms were examined while extensive review of literature on classroom discourse was carried out. When it is assumed that teacher questioning discourse can be categorized into different patterns by considering together the apparent exchange structures and pedagogical functions, various patterns of teacher questioning discourse were revealed. Although most patterns found illustrate the centrality of the teacher, a few of them are considered alternatives to the typical IRE discourse. A framework for classifying teacher questioning discourse is suggested and its implications for science teacher education and future research discussed.

Isolation and Localization of New Germination-related Sequences from Wheat Embryos

  • Caliskan, Mahmut;Bashiardes, Stavros;Ozcan, Birgul;Cuming, Andrew C.
    • BMB Reports
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    • v.36 no.6
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    • pp.580-585
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    • 2003
  • Subtractive library hybridization was used to isolate the cDNA clones that corresponded to the transcripts that were specifically up-regulated during wheat embryo germination. The clones with numbers 5, 6, 7, 8, 24, and 26 appeared to be more abundant in germinating wheat embryos. Among the isolated clones, we identified four new members of the wheat "germin" gene family. We also identified two novel sequences which exhibited distinct germination up-regulation, and displayed characteristic spatial patterns of expression. One of these, represented by clone pSB10, was principally expressed in the root tissue of germinating embryos. The second was represented by the pSB7 clone and was expressed in both the root and shoot primordia of the embryonic axis, as well as within the coleoptile.

A Class Management System on Mobile Platform (모바일 기반 학급관리 시스템)

  • Kim, Sung Jun;Park, Dong Gyu
    • Journal of Korea Multimedia Society
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    • v.19 no.4
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    • pp.780-788
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    • 2016
  • Development of information and communication technologies has given a lot of changes in the business processing methods including the school and class managements. However, many business processing systems are still based on desktop computer. In particular, most of the class management system only supports desktop computers; hence school teachers have difficulty in using their class managements systems on mobile environment. In this paper, we proposed and developed a mobile classroom management system for teachers, which will easily handle information such as personal records of students, student counseling records, student life record and so on. The implementation of the application of mobile-based class management can be managed by many school elements including student register information, student evaluation records and transcripts, counseling report, and so on.