• 동아시아식생활학회지
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• 제16권2호
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• pp.165-173
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• 2006

To investigate the effects of yellow soybean powder on serum lipid level and enzyme activities, male Sprague-Dawley(SD) rats were given hyperlipidemic diets for 6 weeks. Rats(n=8) were divided into three groups consisting the normal group(N), hyperlipidemic diets group(C) and hyperlipidemic diets and yellow soybean powder group(YSP). YSP group lowered triglyceride, total lipid, phospholpid, ester-cholesterol and free-cholesterl than control group. YSP group lowered total-cholesterol, VLDL-cholesterol and LDL-cholesterol but increased HDL-cholesterol of the serum compared to the control group. Athergenic Index(AI) and LHR(LDL-cholesterol/HDL-cholesterol ratio) of YSP group was decreased by control group (p<0.05). On the other hand, glutamic oxaloacetate transaminase(GOT), glutamic pyruvate transaminase (GTP), lactate dehydrogenase(LDHase) and alkaline phosphatase(ALP) enzymes activities were decreased by hyperlipidemic diets group (C). These results suggest that yellow soybean powder fed a lipid lowering effect in serum of rats.

• 한국환경과학회지
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• 제29권4호
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• pp.351-359
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• 2020

The purpose of this study was to investigate the improvement effect of mung bean (Phaseolus aureus L.) on the hepatic functional enzyme and catalase activity of streptozotocin (STZ)-induced diabetic rats. Sprague-Dawley (SD) male rats were divided into four groups (n=6), and fed experimental diets containing mung bean meal [basal diet+5% mung bean (BM), basal diet+STZ+5% mung bean (SM)], and control (Basal Diet, BD), BS groups (basal diet+STZ). Serum concentrations of Blood Urea Nitrogen (BUN) and creatinine were significantly decreased (p<0.05) by 5% mung bean supplementation diet. The activities of aspartate transaminase (AST), alanine transaminase (ALT), akaline phosphatase (ALP), lactate dehydrogenase (LDH), amylase and lipase were decreased in the BD, BM and SM group than BS group. The catalase (CAT) activity was significantly increased (p<0.05) in mung bean supplementation diet (BM, SM group) than diabetic group (BS). In vivo experiments with diabetic rats showed that ingestion of mung bean supplementation diet were effective in BUN concentration, and hepatic functional enzyme activities.

• 한국식품영양과학회지
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• 제28권1호
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• pp.199-204
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• 1999

This study has attempted to examine the effect of Artemisia iwayomogi extract on antioxidant and liver function related enzymes in rats fed high fat diet along with B( )P administration. The activities of the serum glutamic oxaloacetic transaminase, glutamic pyruvate transaminase and alkaline phosphatase of the rats fed Artemisia iwayomogi ethanol extract were decreased compared to the control. Similarily, the activities of the enzymes were also decreased when the combination of B( )P and ethanol extracts were administered compared to the group adminstered only B( )P. On the other hand, high fat diet increased the above liver function related enzymes. The activities of antioxidant enzymes including GST, catalase and Cu,Zn SOD were significantly increased by feeding the extracts (p<0.01) in addition to the increase of tocopherol contents in the serum. These results suggest that Artemisia iwayomogi extracts can protect cell membranes from the damages by free radicals or hydroperoxides and further may lead to the protection from cancer risks.

• Journal of Nutrition and Health
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• 제32권5호
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• pp.519-525
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• 1999

The effects of Ganoderma Lucidum consumption (GL) on the liver function and lipid metabolic disorder induced by alcohol consuming were studied in rats using GL powder(1%, 2%, 4%) and 10% ethly alcohol. Thirty-five rats(Sprague-Dawley, male) were divided into five groups and fed experimental diets for seven weeks. The concentrations of serum cholesterol, HDL-cholesterol and triglyceride were the highest in the alcohol group, while the levels were apparently reduced by in groups fed GL. The atherogenic indices(AI) also tended to be higher in the alcohol group. Glutamic oxaloacetic transaminase(GOT) activities were significantly lower in body alcohol + 2% GL and alcohol + 4% GL groups, and glutamic pyruvic transaminase(GPT)activities lower only in the alcohol + 2% GL compared with the alcohol group. Alkaline phosphatase(ALP) activities were not significantly different among the groups. The elevated levels of liver cholesterol and triglyceride due to alcohol consumption, were apparently reduced by GL supplementation. On microscopic observation of liver tissues, fat droplets appeared extensively on the liver-lobule in the alcohol group, while fat droplets appeared only on the central vein in the alcohol + 1% GL group. However, in groups administered 2% and 4% GL, fat droplets appeared similar to the alcohol group. The concentrations of fecal bile acid were significantly increased in groups fed GL. These results indicate that GL exerted some beneficial effects on lipid metabolic disorder caused by long-term alcohol consumption. Thus, it seems that GL may be effective in preventing or curing some aspects of alcohol toxicity. More detailed studies are needed in order to determine proper dietary levels of GL for combating alcohol toxicity.

• Journal of Radiation Protection and Research
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• 제18권2호
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• pp.61-69
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• 1993

우라늄 피폭후 효소활성도가 내부피폭의 biomarker로서의 가능성을 연구하기 위하여 수서생물인 잉어(Caprinus carpio)의 복강내 우라늄 투여시 간조직에서 일어나는 여러가지 효소계의 변화를 조사하였다. 이러한 효소활성도의 변화는 우라늄 내부피폭의 biomarker로서 가능성을 가지고 있으며 연구결과는 다음과 같다. 1) 6일째까지 우라늄을 주사하여 적출한 간에서의 전체단백질량은 계속 감소하고 있다. 2) 세포내의 lysosome내에 함유하고 있는 acid pretense와 ${\beta}-glucuronidase$의 활성도는 6일째 투여후까지 활성도가 감소하고 있다. 3) Alkaline phosphatase의 경우 6일째 우라늄 투석후까지 증가하고 있으며 반대로 acid phosphatase의 경우 6일째 우라늄 투여후까지 활성도가 급격히 증가하였고 glutamate oxaloacetate transaminase의 활성도는 완만하게 증가하고 있다. 4) Creatine kinase의 활성도는 완만한 감소를 보이고 있으며 malate dehydrogenase는 첫번 우라늄 투여후에 활성도가 급격히 감소하였고 3일째 우라늄 투여후에는 활성도가 거의 나타나지 않았다.

• 생명과학회지
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• 제10권5호
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• pp.490-495
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• 2000

규산염 광물질인 맥반석을 3%수준으로 식이중에 첨가하여 십자매에 2주간 급여한 후 생리적 및 생화학적 성상에 미치는 영향에 대하여 검토하였다. 체중 증가량, 식이 섭취량, 식이 효율, 상대적 간장 및 심장 무게는 대조군과 맥반석 첨가군 사이에 유의적인 차이가 없었고, 상대적 신장 및 근위 무게는 맥반석 첨가군에서 각각 증가 및 감소하였다. 혈청 총 단백질, 혈당, 알부민, 중성지질, 콜레스테롤 농도는 두군 사이에 차이가 없었다. 간기능 지표인 GOT및 ALP 활성은 맥반석 첨가군에서 각각 증가 및 감소하였으나, GTP 활성은 유의적인 차이가 없었다. 따라서, 맥반석 3% 수준의 식이중첨가는 십자매의 생리적 및 생화학적 상태에는 영향을 미치지 않으며, 식이의 영양효율을 증가시키는 것으로 사료된다.

• 한국미생물·생명공학회지
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• 제47권1호
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• pp.139-147
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• 2019

Glutamate decarboxylase catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA), contributing to pH homeostasis through proton consumption. The reaction is the first step toward the GABA shunt. To date, the enzymes involved in the glutamate metabolism of Photobacterium damselae subsp. piscicida have not been elucidated. In this study, an open reading frame of P. damselae subsp. piscicida, showing homology to the glutamate decarboxylase or putative pyridoxal-dependent aspartate 1-decarboxylase genes, was isolated and cloned into an expression vector to produce the recombinant enzyme. Preliminary gas chromatography-mass spectrometry characterization of the purified recombinant enzyme revealed that it catalyzed not only the decarboxylation of glutamate but also the transamination of GABA. This enzyme of P. damselae subsp. piscicida could be bifunctional, combining decarboxylase and transaminase activities in a single polypeptide chain.

• 대한수의사회지
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• 제16권8_10호
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• pp.293-295
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• 1980

Hepatic and renal funtions were observed in normal shephered dogs anesthetized with methoxyflurane for two hours by inhalation techniques used in veterinary practice. Serum glutamicpyruvic transaminase (SGPT) and alkaline phosphotase (ALP) activities were

• 동의신경정신과학회지
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• 제13권2호
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• pp.195-211
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• 2002

Gagamguibitang, a composite Korean medicinal drug prescribed by us, was evaluated for its sedative effects by measurements of potentiation on pentobarbital-induced sleeping time, anticonvulsive activities in animal model, inhibitory effect on GABA transaminase activity, and antioxidative activities in vitro- and/or in vivo assay. The results were summerized as follows : 1. Gagamguibitang showed about 2-fold prolongation of pentobarbital-induced sleeping time compared to the control group after administration(p.o) with 2.0g/kg of mice body weight. 2. Gagamguibitang strongly lengthened onset time of pentylenetetrazole-induced convulsion, shortened the duration of convulsion and diminished the lethality after treatment(p.o) with 1.0g/kg of mice body weight. 3. Gagamguibitang inhibited dose-dependently the brain GABA transaminase activity in vitro compared to the control group and in vivo compared to the pentylenetetrazole-treated group. 4. Gagamguibitang inhibited effectively brain lipid peroxidation by 45.8% at a dose of l0mg/ml in vitro and by 47.5% after oral treatment with 0.5g/kg of mice body weight in vivo assay. 5. Gagamguibitang exhibited a potent scavenging activity on DPPH radical in a dose-dependent manner with ca. 92% activity at l0mg/ml. As a result, Gagamguibitang can be useful for the effective sedative drug in clinical application.

• 방사선산업학회지
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• 제5권2호
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• pp.125-130
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• 2011

The purpose of this study was to evaluate the protective effect of the Perilla frutescens cv. Chookyoupjaso mutant water extract (PFWE) on gamma ray-induced oxidative stress in mice. Gamma-ray is one of the sources for inducing oxidative stress. The study was divided into 6 groups with 6 mice for each treatment. Groups I and II were treated with saline (vehicle) only, groups III, IV, V, and VI were pretreated with PFWE 10, 20, 50, $50mg\;kg^{-1}$ respectively for 2 weeks before gamma radiation. And then groups II, III, IV, V were irradiated. We found that the activities of aspartate transaminase (AST) and alanine transaminase (ALT) were increased and the activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) were decreased by irradiation in mice. However, treatment of PFWE attenuated the activities of AST and ALT in a dose-dependent manner in irradiated mice. Furthermore, treatment of PFWE significantly increased the activities of SOD, GPx, and GR in a dose-dependent manner in irradiated mice, except for the CAT. Interestingly, the activities of GPx and GR were significantly increased by PFWE treatment. Taken together, PFWE could be effective in protecting on gamma ray-induced oxidative stress in mice.