• Biomolecules & Therapeutics
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• 제19권2호
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• pp.201-205
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• 2011

Although various biological activities of resveratrol have been extensively studied, most reports have focused on trans-resveratrol and little attention has been paid to the cis-isomer. In this study, the effect of cis-resveratrol on platelet activity was examined and compared with that of the trans-isomer. Treatment with cis-resveratrol resulted in inhibition of platelet aggregation induced by thrombin, collagen or ADP, which are representative aggregation-inducing agents, and the trans-isomer elicited the same effects. These effects were concentration-dependent in the range of 1-100 ${\mu}M$. However, the potency of the cis-isomer was much lower than that of the trans-isomer; the $IC_{50}$ values for the cis-isomer versus the trans-isomer were $31{\pm}12$ vs $151{\pm}3$, $161{\pm}3$ vs $91{\pm}4$, and $601{\pm}15$ vs $251{\pm}6\;{\mu}M$ for thrombin-, collagen- and ADP-induced aggregation, respectively. These results indicate that cis-resveratrol has a less potent anti-platelet activity, compared with the trans-isomer, and raise the possibility that the biological activities of the cis-isomer may be different from those of the trans-isomer. It will be necessary to evaluate the activity of cis-resveratrol independently of the trans-isomer.

• 한국자원식물학회지
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• 제20권6호
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• pp.558-562
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• 2007

The high-performance liquid chromatography(HPLC) method for the determination of trans-resveratrol in 34 germplasms and processing methods of peanut seeds has been modified. Peanut germplasms contained trans-resveratrol contents of $0.14{\sim}4.96{\mu}g/g$, but findings for the testa color were not significant. However, two germplasms, 'KIGAN' and 'CS1', contained more trans-resveratrol contents than the other germplasms. The contents of their were $2.26{\mu}g/g\;and\;4.96{\mu}g/g$. The tested processing methods caused no significant changes in trans-resveratrol contents. The contents of fresh, boiled, and roasted peanuts were 0.36, 0.32, and $0.40{\mu}g/g$, respectively in cv. Palkwang, and 0.22, 0.22, and $0.26{\mu}g/g$, respectively, in cv. Jakwang. Differences were not significant among fresh, boiled, and roasted peanuts. The grains of 'Palkwang' and 'Jakwang' contained trans-resveratrol contents of $0.34{\mu}g/g\;and\;0.24{\mu}g/g$, and testa contained $1.12{\mu}g/g\;and\;1.00{\mu}g/g$, respectively. However, when comparing absolute quantity, the trans-resveratrol contents appears to be approximately $3{\sim}4$ times higher in the testa than in the grain of the peanut, although the total contents were not different because the ratio of testa was low in peanut seeds.

• 분석과학
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• 제16권2호
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• pp.125-133
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• 2003

적포도주에 들어 있는 trans-resveratrol을 분석하기 위하여 본 연구에서는 Sep-Pak $C_{18}$-cartridges를 이용한 고체상 추출법의 전처리 및 LC/MS-ESI 방법과 LC/MS-APCI 방법을 사용하여 국내에서 시판되고 있는 포도주에서 trans-resveratrol의 함량을 비교 분석하였다. LC/MS-ESI 방법에 의한 trans-resveratrol 함량은 $0.06-4.31{\mu}g/mL$로 검출되었고, 회수율은 88.4-97.9%, 상대 표준편차는 0.6-4.6%로 나타났으며, 검출한계는 $0.001{\mu}g/mL$이었다. LC/MS-APCI방법의 경우 trans-resveratrol 함량은 $0.09-4.02{\mu}g/mL$가 검출되었고, 검출한계는 $0.005{\mu}g/mL$로 나타났다.

• Journal of Microbiology and Biotechnology
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• 제9권5호
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• pp.691-693
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• 1999

Resveratrol (trans-3,5,4'-trihydroxystilbene), a phenolic substance present in both grapes and wines, has been reported to have certain pharmacological effects. Using an ethylacetate-phase extraction followed by a high performance liquid chromatographic analysis, the concentration of trans-resveratrol was measured in 9 red wines commercially available in Korea, including the Korean red wine (Noul). Noul red wine with a 1998 vintage had a trans-resveratrol concentration of 3.3 ㎎/l, which was rather higher than the concentrations found in other wines (0.19-2.45 ㎎/l) with the exception of the French wine, J. P. Chenet (3.39 ㎎/l). In addition, the grapes of Vitis labrusca cv. Sheridan cultivated in the Kyungsan area showed a trans-resveratrol content of 6.4 ㎎/㎏.

• 한국작물학회지
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• 제48권6호
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• pp.429-433
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• 2003

본 연구에서는 땅콩의 resveratrol에 대한 체계적인 연구를 위하여 고속액체크로마토그래피HPLC)를 이용한 땅콩의 trans-resveratrol 분석체계 확립과 품종 및 생육시기별 trans-resveratrol 함량 변화를 구명하여 금후 고부가가치 고함유 기능성 품종개발의 기초 자료로 활용하고자 수행한 결과를 요약하면 다음과 같다. 1. HPLC를 이용한 trans-resveratrol 분석조건은 컬럼은 Xterra $\textrm{C}_{18}$, 이동상 용매조성은 (A) glacial acetic acid DCW(52.6 : 900)과 (B) acetonitril solution A(80:20), 용매흐름속도는 1.1 ml/min, 검출기는 UV-Vis detector 308 nm 이었다. 2. 땅콩에서 trans-resveratrol 추출시 추출용매의 경우 80%EtOH과 80%MeOH이 유의차가 없었고, 추출온도의 경우에도 온도간 유의성이 인정되지 않았으며, ICH guideline에 따른 안전성과 경제성을 고려한 trans-resveratrol의 최적추출 조건은 80% EtOH, $25^{\circ}C$, 45분이었다. 3. 땅콩 품종별 trans-resveratrol 함량 분포범위는 0.018-1.125 $\mu\textrm{g}/\textrm{g}$으로 품종별 유의차가 있었으나, 초형과 립 크기에 따른 trans-resveratrol 함량은 일정한 경향을 보이지 않았다. 4. 종실과 종피를 포함한 종실의 trans-resverarol함량은 종실(0.152-0.305 $\mu\textrm{g}/\textrm{g}$) 보다 종피를 포함한 종실(0.188-0.345 $\mu\textrm{g}/\textrm{g}$)에서 더 높았다. 5. 생육시기별 trans-resveratrol 함량은 신남광땅콩(0.227$\mu\textrm{g}/\textrm{g}$ 대광땅콩(0.418 $\mu\textrm{g}/\textrm{g}$)은 파종 후 110일에 팔광땅콩(0.486 $\mu\textrm{g}/\textrm{g}$)과 밀양16호(0.441 $\mu\textrm{g}/\textrm{g}$)는 파종 후 130일에 가장 높았다.

• 한국식품과학회지
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• 제35권5호
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• pp.764-768
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• 2003

본 연구는 생물학적, 비생물학적인 스트레스에 대한 방어물질중의 한가지인 trans-resveratrol를 포도와 포도가공품(포도주, 포도쥬스)으로부터 분석하는 방법을 확립하였다. trans-Resveratrol 기기분석 조건은 다음과 같다. 분석 column은 Nucleosil 100-5 C18을 사용하였으며 이동상은 acetonitrile: water(40:60, v/v)로서 UV검출기(306 nm)를 이용하여 0.3 mL/min의 유속으로 측정하였다. 분석한 결과, 재현성과 반복성의 CV%는 10%이하였으며, 회수율은 $90{\sim}110%$이었다. 또한, 포도, 포도주 그리고 포도쥬스의 최소검출한계는 14.5 ng/g, 3.62 ng/mL 그리고 4.02 ng/mL이고, 최소정량한계는 14.8 ng/g, 3.69 ng/mL 그리고 4.10 ng/mL이었다. 표준물질인 trans-resveratrol 농도가 $0.048{\sim}2.4\;{\mu}g/mL$일때 $R^2$ 값은 0.999이었다. 32품종의 포도, 9종류의 포도주 그리고 9종류의 포도쥬스 내의 trans-resveratrol 함량은 각각 $0.76{\sim}207.14\;{\mu}g/100\;g$, $5.41{\sim}275.66\;{\mu}g/L$ 그리고 $63.25{\sim}751.55\;{\mu}g/L$로 분석되었다.

• Preventive Nutrition and Food Science
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• 제7권4호
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• pp.447-450
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• 2002

Previously, a methanol extract from seeds of Paeonia lactiflora was shown to have a potent inhibitory activities against tyrosinase and soybean lipoxygenase (SLO). Seven stilbenes, trans-resveratrol-4-Ο-$\beta$-D-glucoside, trans resveratrol, trans-$\varepsilon$-viniferin, cis-$\varepsilon$-viniferin, gnetin H, suffruticosol A and B were isolated from the seeds as active principles for inhibition of the enzymatic activity. Among them, the resveratrol trimer, gnetin H exhibited the most potent inhibitory activities against tyrosinase and SLO, respectively. Additionally, the resveratrol dimers, trans-$\varepsilon$-viniferin and cis-$\varepsilon$-viniferin exhibited significant inhibitory activity against the two oxidative enzymes. Meanwhile, three other stilbene derivatives, such as trans-resveratrol, suffruticosol A and suffruticosol B had also weak inhibition activity. The least inhibitory activity was observed in transresveratrol-4-Ο-$\beta$-D-glucoside. These results suggest that resveratrol dimers and trimer in the seeds of Paeonia lactiflora are potentially useful therapeutic agents against pathological disorders such as hyperpigmentation and inflammation.

• Biomolecules & Therapeutics
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• 제20권3호
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• pp.306-312
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• 2012

Resveratrol (trans-3,5,4'-trihydroxystilbene) has received considerable attention recently for the potential neuroprotective effects in neurodegenerative disorders where heme oxygenase-1 (HO-1) and sirtuin 1 (SIRT1) represent promising therapeutic targets. Resveratrol has been known to increase HO-1 expression and SIRT1 activity. In this study, the effects of resveratrol and trans-3,5,4'-trimethoxystilbene (TMS), a resveratrol derivative, on cytotoxicity caused by glutamate-induced oxidative stress, HO-1 expression, and SIRT1 activation have been investigated by using murine hippocampal HT22 cells, which have been widely used as an in vitro model for investigating glutamate-induced neurotoxicity. Resveratrol protected HT22 neuronal cells from glutamate-induced cytotoxicity and increased HO-1 expression as well as SIRT1 activity in a concentration-dependent manner. Cytoprotection afforded by resveratrol was partially reversed by the specific inhibition of HO-1 expression by HO-1 small interfering RNA and the nonspecific blockage of HO-1 activity by tin protoporphyrin IX, but not by SIRT1 inhibitors. Surprisingly, TMS, a resveratrol derivative with methoxyl groups in lieu of the hydroxyl groups, and trans-stilbene, a non-hydroxylated analog, failed to protect HT22 cells from glutamate-induced cytotoxicity and to increase HO-1 expression and SIRT1 activity. Taken together, our findings suggest that the cytoprotective effect of resveratrol was at least in part associated with HO-1 expression but not with SIRT1 activation and, importantly, that the presence of hydroxyl groups on the benzene rings of resveratrol appears to be necessary for cytoprotection against glutamate-induced oxidative stress, HO-1 expression, and SIRT1 activation in HT22 neuronal cells.

• 한국식품영양과학회지
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• 제43권2호
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• pp.243-249
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• 2014

몇 가지 식물에서 발견되는 항독성 물질(phytoalexin)인 trans-resveratrol(3,5,4'-trihydroxy-trans-stilbene)은 항암, 항산화효과 및 심혈관질환 등에 효과가 큰 물질로 알려져 있으며, 포도, 땅콩 및 오디 등의 발효 시에 증가되어 그 효과가 커지는 것으로 알려져 있다. 한편 stachyose 및 raffinose 등의 기능성 올리고당은 장내 미생물의 생육을 도와 정장작용을 하는 것으로 알려져 있다. 따라서 본 연구에서는 3품종의 대두를 이용하여 제조한 청국장의 발효 중 trans-resveratrol과 기능성 올리고당의 함량 변화를 조사하였으며, 이에 따른 항산화효과를 알아보려고 하였다. trans-Resveratrol 함량은 원료 콩보다는 발효되었을 때 대체로 발효기간이 길어질수록 그 함량이 높게 나타났다. 특히 48시간 발효 후에는 3품종의 콩에서 늘찬, 대원 및 태광의 순으로 각각 $50.06{\pm}0.82$, $39.04{\pm}0.49$, $34.00{\pm}0.54{\mu}g/g$이었으며, 원료 콩에 비하여 4배 이상 증가하였다. Stachyose 함량은 원료 콩에서 $10.84{\pm}0.42{\sim}13.05{\pm}0.13mg/g$, 증자 직후에는 $11.37{\pm}0.03{\sim}12.05{\pm}0.52mg/g$이었으나, 발효 12시간 후에는 $0.16{\pm}0.01{\sim}0.33{\pm}0.02mg/g$으로 원료 콩에 비하여 97% 이상이 줄어들었으며 발효 24시간 후부터는 전혀 검출되지 않았다. 한편 raffinose의 함량은 원료 콩에서 $2.66{\pm}0.09{\sim}3.54{\pm}0.05mg/g$으로 가장 낮았고, 증자 직후부터 발효 24시간 후에는 $10.61{\pm}0.16{\sim}12.66{\pm}0.17mg/g$으로 약 3~4배가량 증가하였으나 48시간에서는 오히려 $8.28{\pm}0.17{\sim}11.83{\pm}0.44mg/g$으로 감소하는 경향을 보였다. 청국장의 발효기간에 따른 항산화효과는 FRAP, DPPH, ABTS assay 모두에서 증자 직후에는 원료 콩에서보다 다소 낮게 나타났으나 대체로 발효기간이 길어짐에 따라 활성이 높아지는 경향이었다. 이와 같은 경향은 trans-resveratrol의 항산화활성이 DPPH, ABTS와 FRAP assay 등에서 $RC_{50}$ 값이 $4.71{\pm}0.36{\sim}8.46{\pm}0.05{\mu}g/mL$인 것을 미루어 볼 때, 발효기간에 따라 유의적으로 증가한 trans-resveratrol의 영향이 일부 있었을 것으로 생각되었다. 그러나 발효 중 기능성 올리고당(stachyose 및 raffinose)의 변화는 청국장의 항산화효과와는 관련이 없는 것으로 나타났다.

• The Korean Journal of Physiology and Pharmacology
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• 제28권4호
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• pp.345-359
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• 2024

Deposition of extracellular matrix (ECM) in the trabecular meshwork (TM) increases aqueous humour outflow resistance leading to elevation of intraocular pressure (IOP) in primary open-angle glaucoma, which remains the only modifiable risk factor. Resveratrol has been shown to counteract the steroid-induced increase in IOP and increase the TM expression of ECM proteolytic enzymes; however, its effects on the deposition of ECM components by TM and its associated pathways, such as TGF-β-SMAD signalling remain uncertain. This study, therefore, explored the effects of trans-resveratrol on the expression of ECM components, SMAD signalling molecules, plasminogen activator inhibitor-1 and tissue plasminogen activator in dexamethasone-treated human TM cells (HTMCs). We also studied the nature of molecular interaction of trans-resveratrol with SMAD4 domains using ensemble docking. Treatment of HTMCs with 12.5 µM trans-resveratrol downregulated the dexamethasone-induced increase in collagen, fibronectin and α-smooth muscle actin at gene and protein levels through downregulation of TGF-β1, SMAD4, and upregulation of SMAD7. Downregulation of TGF-β1 signalling by trans-resveratrol could be attributed to its effect on the transcriptional activity due to high affinity for the MH2 domain of SMAD4. These effects may contribute to resveratrol's IOP-lowering properties by reducing ECM deposition and enhancing aqueous humour outflow in the TM.