• Journal of Ginseng Research
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• 제42권3호
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• pp.343-351
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• 2018

Background: Red ginseng is a popularly used traditional medicine with antiaging effects in Asian countries. The present study aimed to explore the changes in protein expression underlying the mechanisms of life span extension and antiaging caused by red ginseng extract (RGE) in Drosophila melanogaster. Methods: A proteomic approach of two-dimensional polyacrylamide gel electrophoresis (2-DE) was used to identify the differential abundance of possible target proteins of RGE in D. melanogaster. The reliability of the 2-DE results was confirmed via Western blotting to measure the expression levels of selected proteins. Proteins altered at the expression level after RGE treatment (1 mg/mL) were identified by matrix-assisted laser desorption/ionization-time of flight tandem mass spectrometry and by searching against the National Center for Biotechnology nonredundant and Uniprot protein databases. The differentially expressed proteins were analyzed using bioinformatics methods. Results: The average survival life span of D. melanogaster was significantly extended by 12.60% with RGE treatment (1 mg/mL) compared to untreated flies. This followed increased superoxide dismutase level and decreased methane dicarboxylic aldehyde content. Based on the searching strategy, 23 differentially expressed proteins were identified (16 up-regulated and 7 down-regulated) in the RGE-treated D. melanogaster. Transduction pathways were identified using the Kyoto Encyclopedia of Genes and Genomes database, and included the hippo and oxidative phosphorylation pathways that play important roles in life span extension and antiaging process of D. melanogaster. Conclusion: Treatment with RGE in D. melanogaster demonstrated that mechanisms of life span extension and antiaging are regulated by multiple factors and complicated signal pathways.

• 디지털융복합연구
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• 제17권10호
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• pp.427-432
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• 2019

본 연구의 목적은 환경 문제에 대한 시대적 배경과에 맞추어 일본의 대표적인 친환경 건축가인 후지모리 테루노부 작품에 나타나는 공간표현 특성을 알아보고자한다. 연구 방법으로는 1990년대부터 현재까지의 작품 중 그를 대표하는 다실 공간 5곳을 선정하여 문헌연구를 통하여 공간의 표현 특성을 알아보고자 한다. 분석결과로는 반드시 자연소재를 사용하여 공간을 마감 하고 건축물에 식물을 도입하는 전략을 적극적으로 사용하고 있었다. 일본의 전통방식을 차용하는 전략은 공상성과 일탈성을 나타내는 공간표현 방식으로 사용하고 있었다. 이 시대에 새로움을 추구하는 현대인들의 요구와 실현 가능한 대안의 친환경 건축 계획 시 또 다른 공간디자인 방법론으로 제시할 수 있다고 기대해 본다.

• 커뮤니케이션디자인학연구
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• 제59권
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• pp.114-127
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• 2017

현재 여백에 대한 연구는 주로 미술학, 건축학 논저에서 찾아볼 수 있으며, 영상 학술 연구 중에서는 비교적 적은편이다. 그러나 여백은 의미를 표현하는 수단으로서 영상 작품에 독특한 예술적 매력을 부여하며, 영화 예술에 있어 여백 형식의 발전은 능동적인 미학적 영향을 미친다. 영상작품 속 여백사상에 대한 깊이 있는 연구를 위해 본문에서는 먼저 '여백'의 정의에 대해서 논의하고 여백이 생겨난 역사와 사회적 기원을 거슬러 올라가서 여백과 도교(道敎)학설의 관계와 중국화 속에서의 응용을 살펴볼 것이다. 다음은 도교에서 말하는 '여백' 사상을 결합하여 영상예술 속에서의 여백의 기능과 예술적 표현, 그리고 사람들의 사상승화에 미친 영향 등을 분석할 것이다. 여백은 하나의 의미표현 수단으로서 미적 가치를 실현함과 동시에 대중의 영화예술 지각능력을 강화한다. 그러하기에 여백이라는 유구한 미학 사상은 새로운 시대, 새로운 영역에서 지속적인 생기를 발산하게 될 것이다.

• 대한본초학회지
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• 제34권1호
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• pp.99-107
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• 2019

Objectives : Cudrania tricuspidata (C. tricuspidata) is well-known traditional herbal remedy and its root, leaf and fruit were used for treatment of inflammation, tumor and painkilling. However, effect of C. tricuspidata fruit on tight junction is still unknown. The aim of this research was to determine the effect of C. tricuspidata fruit extract on human keratinocyte HaCaT cells. Methods : The antioxidant effects of water extract of C. tricuspidata (WECT) and ethanol extract of C. tricuspidata (EECT) were analyzed by using an ABTS assay. To confirm the cytotoxicity of WECT and EECT, MTS assay was performed. The mRNA expression levels of tight junction related genes were analyzed using quantitative RT-PCR analysis. Furthermore, dispase assay was used to investigate the alteration of cell-cell adhesion strength of EECT treated HaCaT cells. Results : WECT and EECT showed strong antioxidant activity. No obvious cytotoxicity was observed in both WECT and EECT until $2.0mg/m{\ell}$ concentration. The mRNA expression level of Claudin 6 were significantly increased by EECT treatment, whereas the WECT did not affect the expression of Claudin 6. Furthermore, EECT treatment enhances cell-cell adhesion strength. Conclusions : In this study, we investigated the physiological activities of the extracts of Cudrania tricuspidata fruit extracts on human keratinocytes by two different extraction methods. EECT might have an anti-aging activity on the skin by reducing oxidative stress. Moreover, it may be a useful ingredient in atopic dermatitis and skin-moisturizing, given its effects of altering Claudin 6 gene expression and enhancing cell-cell adhesion strength.

• The Korean Journal of Physiology and Pharmacology
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• 제27권3호
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• pp.241-256
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• 2023

Although chimeric antigen receptor T cell (CAR-T) is a promising immunotherapy in hematological malignancies, there remain many obstacles to CART cell therapy for solid tumors. Identifying appropriate tumor-associated antigens (TAAs) is especially critical for success. Using a bioinformatics approach, we identified common potential TAAs for CAR-T cell immunotherapy in solid tumors. We used the GEO database as a training dataset to find differentially expressed genes (DEGs) and verified candidates using the TCGA database, obtaining seven common DEGs (HM13, SDC1, MST1R, HMMR, MIF, CD24, and PDIA4). Then, we used MERAV to analyze the expression of six genes in normal tissues to determine the ideal target genes. Finally, we analyzed tumor microenvironment factors. The results of major microenvironment factor analyses showed that MDSCs, CXCL1, CXCL12, CXCL5, CCL2, CCL5, TGF- β, CTLA-4, and IFN-γ were significantly overexpressed in breast cancer. The expression of MST1R was positively correlated with TGF- β, CTLA-4, and IFN-γ. In lung adenocarcinoma, MDSCs, Tregs, CXCL12, CXCL5, CCL2, PD-L1, CTLA-4, and IFN-γ were significantly overexpressed in tumor tissues. The expression of MST1R was positively correlated with TGF- β, CTLA-4, and IFN-γ. In bladder cancer, CXCL12, CCL2, and CXCL5 were significantly overexpressed in tumor tissues. MST1R expression was positively correlated with TGF- β. Our results demonstrate that MST1R has the potential as a new target antigen for treating breast cancer, lung adenocarcinoma, and bladder cancer and may be used as a progression indicator for bladder cancer.

• 대한본초학회지
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• 제39권4호
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• pp.19-28
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• 2024

Objectives : Ephedrae Herba has been used in the East Asian traditional medicine, for treatment of asthma, cold and influenza. Silymarin is an effective antioxidant and its anti-fibrogenic, anti-inflammatory, and hepatoprotective properties have been reported. This study was performed to explore an anti-fibrogenic potential of Ephedrae Herba extract (EHE) + silymarin on immortalized human hepatic stellate cell line, LX-2 cells. Methods : We studied the anti-fibrogenic effects of EHE + silymarin on transforming growth factor β1 (TGF-β1) signaling pathway in LX-2 cells. Cell viability was measured using the MTT assay. mRNA levels were detected by real-time PCR. TGF-β1 signaling-related proteins expression were detected by Western blot. Results : Silymarin 30 ㎍/mL and EHE 100 ㎍/mL showed cytotoxicity on LX-2 cells. Therefore, the concentrations of silymarin and EHE were studied at 10 ㎍/mL, respectively. Silymarin significantly reduced PAI-1 protein expression, Smad binding element (SBE) luciferase activity, and mRNA (PAI-1, MMP2 and 9) expression compared to TGF-β1. EHE significantly reduced SBE luciferase activity and mRNA (PAI-1, MMP2 and 9) expression compared to TGF-β1. More importantly, EHE + silymarin significantly reduced all parameters compared to TGF-β1, and also significantly reduced compared to EHE alone and silymarin alone. Conclusion : The results indicate that EHE + silymarin has anti-fibrogenic effect in LX-2 cells induced by TGF-β1. Additionally, EHE + silymarin shows more effective anti-fibrogenic effect than EHE alone and silymarin alone.

• Journal of Animal Science and Technology
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• 제66권5호
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• pp.936-948
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• 2024

The Conical 9 well dish (C9 well dish) is characterized by a decreasing cross-sectional area towards the base. This design was hypothesized to enhance embryonic development by emulating the in vivo physical environment through density modulation. Comparative analyses revealed no significant difference in nuclear maturation rates between the C9 well dish and the 5-well dish. Reactive oxygen species (ROS) generation was lower in the C9 well dish compared to the 5-well dish; however, this difference was not statistically significant. On the second day of in vitro culture, the cleavage rate in the C9 well dish was 4.66% higher, although not statistically significant, and the rates of blastocyst development were similar across both dishes. No significant differences were observed in the intracellular levels of glutathione (GSH) and ROS, as well as in the total cell number within the blastocysts between the dish types. The expression of mitogen-related factors, TGFα and IGF-1, in the blastocysts was consistent between the dishes. However, PDGFβ expression was significantly lower in the C9 well dish compared to the 35 mm petri dish. Similarly, the expression of the apoptosis factor Bax/Bcl2l2 showed no significant differences between the two dishes. Despite the marked difference in PDGFβ expression, its impact on blastocyst formation appeared negligible. The study also confirmed the feasibility of culturing a small number of oocytes per donor, collected via Ovum Pick-Up (OPU), with reduced volumes of culture medium and mineral oil, thus offering economic advantages. In conclusion, the present study indicates that the C9 well dish is effective for in vitro development of a small quantity of oocytes and embryos, presenting it as a viable alternative to traditional cell culture dishes.

• 조형예술학연구
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• 제10권
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• pp.183-218
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• 2006

Colors change in time corresponding with the value system and aesthetic consciousness of the time. The roles that colors play in painting can be divided into the formative role based on the contrast and harmony of color planes and the aesthetic role expressed by colors to represent the objects. The aesthetic consciousness of the orient starts with the Civility(禮) and Pleasure(樂), which is closely related with restrained or tempered human feelings. In the art world of the orient including poem, painting, and music, what are seen and felt from the objects are not represented in all. Added by the sentiment laid background, the beauty of the orient emphasizes the beauty of restraint and temperance, which has long been the essential aesthetic emotion of the orient. From the very inception of oriental painting, colors had become a symbolic system in which the five colors associated with the philosophy of Yin and Yang and Five Forces were symbolically connected with the four sacred animals of Red Peacock, Black Turtle, Blue Dragon, and White Tiger. In this color system the use of colors was not free from ideological matters, and was further constrained by the limited color production and distribution. Therefore, development in color expression seemed to have been very much limited because of the unavailability and unreadiness of various colors. Studies into the flow in oriental painting show that color expression in oriental painting have changed from symbolic color expression to poetic expression, and then to emotional color expression as the mode of painting changes in time. As oriental painting transformed from the art of religious or ceremonial purpose to one of appreciation, the mast visible change in color expression is the one of realism(simulation). Rooted on the naturalistic color expression of the orient where the fundamental properties of objects were considered mast critical, this realistic color expression depicts the genuine color properties that the objects posses, with many examples in the Flower & Bird Painting prior to the North Sung dynasty. This realistic expression of colors changed as poetic sentiments were fused with painting in later years of the North Sung dynasty, in which a conversion to light ink and light coloring in the use of ink and colors was witnessed, and subjective emotion was intervened and represented. This mode of color expression had established as free and creative coloring with vivid expression of individuality. The fusion of coloring and lyricism was borrowed from the trend in painting after the North Sung dynasty which was mentioned earlier, and from the trend in which painting was fused with poetic sentiments to express the emotion of artists, accompanied with such features as light coloring and compositional change. Here, the lyricism refers to the artist's subjective perspective of the world and expression of it in refined words with certain rhythm, the essence of which is the integration of the artist's ego and the world. The poetic ego projects the emotion and sentiment toward the external objects or assimilates them in order to express the emotion and sentiment of one's own ego in depth and most efficiently. This is closely related with the rationale behind the long-standing tradition of continuous representation of same objects in oriental painting from ancient times to contemporary days. According to the thoughts of the orient, nature was not just an object of expression, but recognized as a personified body, to which the artist projects his or her emotions. The result is the rebirth of meaning in painting, completely different from what the same objects previously represented. This process helps achieve the integration and unity between the objects and the ego. Therefore, this paper discussed the lyrical expression of colors in the works of the author, drawing upon the poetic expression method reflected in the traditional Flower and Bird Painting, one of the painting modes mainly depending on color expression. Based on the related discussion and analysis, it was possible to identify the deep thoughts and the distinctive expression methods of the orient and to address the significance to prioritize the issue of transmission and development of these precious traditions, which will constitute the main identity of the author's future work.

• 한국전통조경학회지
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• 제31권3호
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• pp.75-82
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• 2013

본 연구는 한국전통정원을 조성할 수 있는 기준점 마련과 기반 구축을 위한 기초연구로서, 해외에 조성된 사례를 바탕으로 조성내용과 표현방식에 있어서 현황과 문제점을 규명하는데 목적을 두고 있다. 9개의 사례지를 선정하여 직접 현장조사와 모니터링 평가를 실시하였다. 연구의 결과를 종합하면, 전반적으로 전통의 단순한 재현으로 인한 대중적 보편성과 시대성이 결여되고 있으며, 전통성과 실험적 해석에서의 오류가 나타나고 있었다. 디자인측면에서는 전통정원의 점경물, 장식물 위주의 편집된 디자인 형태로 나타나며, 경관적인 면에서는 전통정원의 공간규모 및 체험위계에 대한 전달이 미흡하고, 주변 경관의 맥락에 대한 고려가 부족하였다. 안내 및 정보전달측면에서는 다양한 명칭으로 인하여 한국정원에 대한 인지성이 결여되어 있어 명칭에 대한 통일이 요구된다. 조성 관리 및 이용측면에서는 공간성격에 적합한 식재 수종과 유사 대체수종에 대한 제안이 필요하고, 이용행태를 고려한 수용적 자세가 요구된다.

• 한국보육지원학회지
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• 제10권5호
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• pp.229-248
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• 2014

본 연구는 유아 다중지능발달에 있어 민속놀이가 효율적으로 활용되고자 하는데 목적을 두고, 유아교육 및 민속놀이 관련 전문가 20명을 대상으로 델파이 조사를 통해 다중지능발달을 위한 민속놀이의 선정기준을 마련한 것이다. 연구결과 언어지능에는 쓰기 듣기 말하기 단어습득-활용, 논리수학지능에는 전략 수세기 패턴 가설-검증 비교-대조 계산능력, 공간지능에는 그리기 색칠 표상활동 조작-만들기, 신체-운동지능에는 대근육 눈과 손의 협응 유연성 조절력 균형 순발력 근력, 음악지능에는 노래 악기연주, 대인관계지능에는 조망수용 역할분담 협동 토의, 개인이해지능에는 개인적 의미-연계 계획-결정 정서표현 문제해결 이타심, 자연탐구지능에는 생물 무생물 계절 일기 천체가 주요 요소로 포함된 문항들이 선정되었다. 또한 선정기준에 따라 민속놀이를 분석한 결과 각각의 민속놀이는 여러 지능에 해당되는 것을 알 수 있었다. 이는 민속놀이가 유아의 다중지능발달을 도모하는데 있어 활용가치가 높다는 것을 시사하는 것이라 할 수 있다.