• Proceedings of the Korean Society for Bioinformatics Conference
• /
• 2006.02a
• /
• pp.78-78
• /
• 2006

Gene expression analysis can be performed by one-color (intensity-based) or two-color (ratio-based) microarray platforms depending on the specific applications and needs of the researcher. The traditional two-color approach is well founded from a historical and scientific standpoint, and the one-color approach, when paired with high quality microarrays and a robust workflow, offers additional flexibility in experimental design. Two of the major requirements of any microarray platform are system reproducibility, which provides the means for high confidence experiments and accurate comparison across multiple samples; and high sensitivity, for the detection of significant gene expression changes, including small fold changes across multiple gene sets. Each of these requirements is fulfilled by the Agilent One-color Gene Expression Platform as illustrated by the data included in this study. As a result, researchers have the ability to take advantage of the enhanced performance and sensitivity of Agilent's 60-mer oligonucleotide microarrays, and experience the first commercial microarray platform compatible with both one- and two-color detection.

• Journal of Aquaculture
• /
• v.22 no.1
• /
• pp.51-55
• /
• 2009

Transcriptional response of the second isoform of glyceraldehyde (GAPDH-2) to infectious challenges using various bacterial species and the rockbream iridovirus (RBIV) was examined in the spleen of the barred knifejaw (Oplegnathus fasciatus). Bacterial challenges of the juvenile barred knifejaws with Escherichia coli, Edwardsiella tarda, Vibrio anguillarum and Streptococcus iniae resulted in the significant elevation of the GAPDH-2 transcripts in the spleen up to 12-fold relative to that in the non-challenged controls (PBS-injected). In addition, the barred knifejaw fingerlings injected with RBIV stock also represented the highly upregulated mRNA expression of the GAPDH-2 up to more than 20-fold when compared to that of control fingerings. Results obtained from this study strongly suggest that the GAPDH-2 is no longer a housekeeping glycolytic protein and rather than that it might be associated with immune-relevant cellular activities. From this finding, the traditional dogma for the use of GAPDH as an invariant standard for gene expression assays should be carefully revised depending on the kinds of biological stimulations applied in this species.

• Journal of the Korea Fashion and Costume Design Association
• /
• v.15 no.1
• /
• pp.47-57
• /
• 2013

In terms of expression, beauty illustration has become more diverse and innovative. Because of continued unique creative activities, more novelty has been pursued. In this study, beauty illustration in new artistic and decorative images was used to investigate the basic concepts of art activities, illustration and beauty illustration and make up for beauty illustration which adheres to traditional and classical expressions. In this study, the following results were obtained: First, in terms of expression of beauty illustration, the work with art expressions are still poor compared to other fields such as fashion illustration. Therefore, it is necessary to attempt beauty illustration of a new image which expresses artistic emotions. Second, by using an artistic technique called 'paper sculpture', a semi-solid shape has been completed, and a decorative beauty illustration was created. People get new inspiration through beautiful and innocent art pieces, which in turn motivate to create new expression. New art activities which take a human body as an art support beauty activities through picture and more sensitive inspiration. It was attempted to express other emotion and beauty by observing a single piece of work. A new possibility in beauty illustration which is differentiated from other art activities is proposed and its domain is developed.

• The Korean Journal of Physiology and Pharmacology
• /
• v.27 no.6
• /
• pp.513-520
• /
• 2023

Cornuside is a secoiridoid glucoside compound extracted from the fruits of Cornus officinalis. Cornuside has immunomodulatory and anti-inflammatory properties; however, its potential therapeutic effects on diabetic nephropathy (DN) have not been completely explored. In this study, we established an in vitro model of DN through treating mesangial cells (MMCs) with glucose. MMCs were then treated with different concentrations of cornuside (0, 5, 10, and 30 μM). Cell viability was determined using cell counting kit-8 and 5-ethynyl-2'-deoxyuridine assays. Levels of proinflammatory cytokines, including interleukin (IL)-6, tumor necrosis factor-α, and IL-1β were examined using enzyme-linked immunosorbent assay. Reverse transcription quantitative real-time polymerase chain reaction and Western blotting were performed to detect the expression of AKT and nuclear factor-kappa B (NF-κB)-associated genes. We found that cornuside treatment significantly reduced glucose-induced increase in MMC viability and expression of pro-inflammatory cytokines. Moreover, cornuside inhibited glucose-induced phosphorylation of AKT and NF-κB inhibitor alpha, decreased the expression of proliferating cell nuclear antigen and cyclin D1, and increased the expression of p21. Our study indicates that the anti-inflammatory properties of cornuside in DN are due to AKT and NF-κB inactivation in MMCs.

• International journal of advanced smart convergence
• /
• v.13 no.2
• /
• pp.148-153
• /
• 2024

This article embarks on an exploration of how the burgeoning landscape of AI technology is reshaping and augmenting creative expression within the realm of 3D animation. As AI continues to evolve and mature, its integration into the process of 3D animation creation has become an increasingly focal point of investigation and analysis. The article undertakes a comprehensive examination of the myriad applications of AI within the domain of 3D animation, shedding light on its multifaceted contributions to various aspects of the creative process. Furthermore, it delves into the transformative impact that AI technology has on enhancing creative expression within 3D animation, particularly through increased productivity, personalized content creation, and the expansion of creative boundaries. By automating repetitive and time-consuming tasks inherent in traditional production methods, AI liberates artists and animators to unleash their creative ingenuity and push the boundaries of their craft. Through empirical research and case studies, the article elucidates how AI serves as a catalyst for innovation, fostering a conducive environment for the exploration of novel techniques and artistic styles.

• Journal of the Korean Society of Costume
• /
• v.65 no.1
• /
• pp.89-103
• /
• 2015

The purpose of this study is to design fashion-cultural products by reviewing the symbolism in Changseung and Sotdae, which are folk belief sculptures. This study was performed by literature reviews of Changseung and Sotdae in order to look into the characteristics of folk belief and traditional culture, and the designs were developed from the symbolism extracted from Changseung and Sotdae with photoshop CS5 and illustrator CS5. The Symbolism of Changseung and Sotdae were as follows: First, Changseung and Sotdae express the 'symbiotic world view' that human beings need to devote one's life to nature. Second, original materials were saved in order to achieve the 'pursuit of essence'. Third, 'simple esthetic expression' was symbolized through the undivided condition between art, religion and life. And fourth, 'empathic humor' was seen through the basis of emotional sense of affinity. The first concept of design development was 'Pursuit of Essence', which was inspired by the characteristics of 'symbiotic world view' and 'pursuit of essence'. Changseungs were expressed as simple and abstract, and Sotdaes as simplified and modern by symmetrical and rotating copy. The second concept, 'Touch of Humor' was inspired by 'simple esthetic expression' and 'emphatic humor'. The face of Changseungs was exaggerated in a humorous ways and Sotdae was developed as sub-motives. The items for the fashion-cultural design were T-shirts, bags, and scarves. Total of 24 items were developed with 4 differently styled designs for each concept. This study was based on basic culture and attempted to diversify the traditional culture items. It hopes to raise the value of traditional culture, and furthermore help build up national competitiveness.

• Journal of the Korea Furniture Society
• /
• v.26 no.4
• /
• pp.456-466
• /
• 2015

Today, a definition of tourism is shifting from simple sightseeing to experiencing various cultures directly and/or indirectly. Along with the shift, all countries of the world dedicate their full attention on globalization project. In order to do so, they try to promote their images and cultures through eating and drinking spaces for food where the rest of the world can approach with ease. China is a country of diverse culinary culture. By analyzing eating and drinking spaces operated in four Chinese cities, this research studies how the traditional Chinese elements are expressed in modernization. With the research, it will navigate a direction as a new design way to establish modernized traditionality in the future. The research conducted an advanced research that set up and analyzed a standard frame of realizing practice for traditionality. By doing so, it sorted out and reviewed the characteristics of interior space into four styles; an indicative imitation method, a partial invoked method, a partial transformation method and a conceptual reception method. According to the outcome of an analysis, (a) the indicative imitation method that directly employed Chinese tradition was most used one, followed by (b) the partial invoked method that carried a part of traditional space, (c) the partial transformation method that simplified or transformed the tradition, and (d) the conceptual reception method that interpreted hidden minds and concepts by illustrating modern spirit over an inherent order in traditional Chinese space, in respective order.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.24 no.4
• /
• pp.624-629
• /
• 2010

Nardostachys jatamansi water extract (NJ) has long been used for the treatment of inflammation-and immune-mediated disorders in the oriental countries. However, its site of action and pharmacological mechanism are not fully investigated. In this study, the authors tried to explore the cytoprotective and anti-inflammatory actions of NJ. First of all, NJ has no harmful effects on viability of neuronal cell line HT22 cells in the dose range of 300 mg/ml. On the contrary, it shows cytoprotective effects on the cells treated with reactive oxygen species H2O2. Probably the cytoprotective effects of NJ might be caused by its ability to induce well known cytoprotective gene hem oxygenase-1 (HO-1). Furthermore, NJ shows inhibitory effects on the expression of inducible nitric oxide synthase (iNOS) and NO production which are known to destroy the integrity of both cells and tissues. It also inhibits potent proinflammatory cytokine tumor necrosis factor-alpha (TNF-a) production. The blocking effects of NJ on cytopathic and proinflammatory actions of LPS might be caused by the induction of cytoprotective and anti-inflammatory genes HO-1 in macrophages cell line RAW 264.7 cells. The results in this study suggest NJ could be used for the amelioration of inflammation which is underlying mechanism responsible for most chronic diseases.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.34 no.2
• /
• pp.336-344
• /
• 2010

Stage costumes are characterized by the importance as a medium of total visual expression on a synthetic art and need to be designed more effectively. This study followed the stage costumes of the play 'Long long time ago whuo-ee whuoee' which participated in the China-Japan-Korea University Theatre Festival. By consisting of the framework of a Korean story that tells and expands the visual expression province by the access method of a producer, the unique costume in the play was created by the dyeing of the traditional hangi. First, the use of hanji in theater represents originality and a fresh variation with the formative beauty of stage costumes in accordance with hanji that conforms to the recreation of tradition. Second, the dip dyeing technique made it possible to express colors limitlessly and the airbrush dyeing technique along with dyeing twice could account for the weak points of the color. As a result, the color brought effect as expected. Third, the weak points were supported by a Jumchi technique that created the fibroid material tangled with stronger adhesion in the creation of a hangi costume. In addition, dyed hangi was attached to the outer fabric. It allowed the costume to have a unique texture that was both soft and tough. This enforced the visualization of the costume and durability to prevent possible damage by the performers. Forth, pieces of hanji were attached to the damaged parts of the costume after re-visiting the dress rehearsal. As a result, the stage costumes represented the shabby clothes that express the social class of the characters. A new understanding of the excellence of traditional hanji was found and confirmed the possibility of it as a material for stage costumes through the study.

• Journal of Digital Convergence
• /
• v.12 no.11
• /
• pp.505-513
• /
• 2014

Modern metal craft is losing our tradition and personality with simple expressive methods and limited techniques without artistry with the purpose of mass production. In order for development of modern metal craft and to succeed our unique culture, there is a need to consider traditional craft techniques that have been delivered since the ancient. Inlay craft requires high concentration and technology, and it is typical traditional metal craft techniques that show contemporary culture and ideas well. the period of the Three States was the time when inlay technique was introduced the first, and it can be seen in Baekje, Gaya and Silla. Furthermore, when inlay craft is applied for modern metal craft, a possibility was found to grant artistic expression and historical value of our unique artistic expression and historical value. For this purpose, it is expected to establish development of modern metal craft and our unique individuality firmly when continuously seeking practical measures that can accept contemporary crafts and expanding opportunities of education to learn traditional techniques.