• Journal of Ginseng Research
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• 제42권3호
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• pp.270-276
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• 2018

Background: Notoginsenoside Ft1 is a promising potential candidate for cardiovascular and cancer disease therapy owing to its positive pharmacological activities. However, the yield of Ft1 is ultralow utilizing reported methods. Herein, an acid hydrolyzing strategy was implemented in the acquirement of rare notoginsenoside Ft1. Methods: Chemical profiles were identified by ultraperformance liquid chromatography coupled with quadruple-time-of-flight and electrospray ionization mass spectrometry (UPLC-Q/TOF-ESI-MS). The acid hydrolyzing dynamic changes of chemical compositions and the possible transformation pathways of saponins were monitored by ultrahigh-performance LC coupled with tandem MS (UHPLC-MS/ MS). Results and conclusion: Notoginsenoside Ft1 was epimerized from notoginsenoside ST4, which was generated through cleaving the carbohydrate side chains at C-20 of notoginsenosides Fa and Fc, and vinaginsenoside R7, and further converted to other compounds via hydroxylation at C-25 or hydrolysis of the carbohydrate side chains at C-3 under the acid conditions. High temperature contributed to the hydroxylation reaction at C-25 and 25% acetic acid concentration was conducive to the preparation of notoginsenoside Ft1. C-20 epimers of notoginsenoside Ft1 and ST4 were successfully separated utilizing solvent method of acetic acid solution. The theoretical preparation yield rate of notoginsenoside Ft1 was about 1.8%, which would be beneficial to further study on its bioactivities and clinical application.

• 대한예방한의학회지
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• 제15권2호
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• pp.1-19
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• 2011

Toxicokinetics of Korean Traditional Medicines(TKM) is the description of what rate TKM will enter the body and what happens to it once it is in the body in terms of toxicology. However, it is not easy to understand TKM toxicokinetics because of various factors such as a mixture of 2-30 kinds of herbal materials containing thousands of chemicals, and complex chemical properties. For these reasons, little is known about toxicokinetics of TKM. This study was aimed to characterize and review the absorption, distribution and metabolism of korean traditional medicines in a view of toxicokinetics. For this aim, some of korean traditional medicines were reviewed on a basis of drug-drug interaction, biotransformation and intestinal metabolisms by bacteria. As the factors affecting mainly on toxicokinetics of TKM, individual herbal material's degree of lipophilicity and metabolic rate, and decoction components according to different kinds of herbal materials were considered. Other factors such as intestinal pH and bacterial activity for metabolism affecting on TKM toxicokinetics, especially in small intestine. It would be a better way for improving the adverse or poor effects caused by TCM if the factors affecting on toxicokinetics of TKM is considered.

• Natural Product Sciences
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• 제17권1호
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• pp.33-37
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• 2011

The plant species reported here are used by traditional healers in Gabon for different ailments such as wounds, malaria, fever, gonorrhea or diarrhea. The aim of this study was to evaluate the antimicrobial activities of 7 plants (Strombosiopsis tetrandra, Tetraberlinia bifoliolata, Dichapetalum barbatum, Guibourtia demeusii, Dacryodes normandii, Manniophytum fulvum, Paropsia grewoides) against different strains of both Methicillin-Resistant Staphylococcus aureus (MRSA) and Salmonella. Disc diffusion was first used to determine the antimicrobial effectiveness of the plants' ethanolic extracts. Then the minimum inhibitory concentrations of the crude extracts of either leaves or stem barks of the 7 plants were determined using broth micro-dilution. The ethanolic plant extracts showed very good activity against both MRSA and Salmonella strains where the MICs ranged from $250\;{\mu}g/ml$ to $1000\;{\mu}g/ml$. The study shows that many of the tested plants used by Gabonese traditional healers have antimicrobial activities and give support to their traditional use.

• Natural Product Sciences
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• 제10권4호
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• pp.152-167
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• 2004

Traditional Chinese Medicines (TCM) have attracted great interest in recent researchers as alternative medicines for incurable diseases. This review focuses on qualitative and quantitative analytical approaches for bioactive metabolites of components flavonoids and saponins of traditional Chinese medicines by TLC system, although various methods have been introduced. Emphasis will be put on the processes of metabolite extraction from intestinal bacterial cultures or urines, separation (mobile phase) and detection. The identified metabolites by selection of extraction solvent and detection methods are also discussed. In addition, metabolite determinations of flavonoids (baicalin, apiin, rutin, quercetin, quercitrin, kaempferol, diosmin, hesperidin, poncirin, naringin, puerarin, daidzin, daidzein, tectoridin) and saponins (ginsenosides, kalopanaxsaponins, glycyrrhizin, chiisanoside, saikosaponins, soyasaponins) in culture fluid, in urine and in some herbal formula extracts are summarized. These bioactive metabolites of these components by intestinal microflora should be connected to pharmacological actions.

• 생약학회지
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• 제40권4호
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• pp.388-393
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• 2009

Advanced glycation end products (AGEs) have been implicated in the development of diabetic complications. The AGEs inhibitors or cross-link breakers attenuate various functional and structural manifestations of diabetic complications. In this study, 64 herbal medicines from China and Vietnam have been investigated with an in vitro evaluation system using AGEs inhibitory activity. Of these, eight herbal medicines ($IC_{50}$<50 ${\mu}g$/ml) were found to have strong AGEs inhibitory activity compared with aminoguanidine (14 days, $IC_{50}$=75.98 ${\mu}g$/ml; 28 days, $IC_{50}$=88.27 ${\mu}g$/ml). Particularly, four herbal medicines, Buddleja officinalis (whole plant), Syzygium cuminii (leaf), Eugenia caryophyllate (seed), and Paeonia suffruticosa (root) showed more potent inhibitory activity (approximately 5-6 fold) than the positive control aminoguanidine.

• Journal of Ginseng Research
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• 제45권1호
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• pp.1-21
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• 2021

Panax species have gained numerous attentions because of their various biological effects on cardiovascular, kidney, reproductive diseases known for a long time. Recently, advanced analytical methods including thin layer chromatography, high-performance thin layer chromatography, gas chromatography, high-performance liquid chromatography, ultra-high performance liquid chromatography with tandem ultraviolet, diode array detector, evaporative light scattering detector, and mass detector, two-dimensional high-performance liquid chromatography, high speed counter-current chromatography, high speed centrifugal partition chromatography, micellar electrokinetic chromatography, high-performance anion-exchange chromatography, ambient ionization mass spectrometry, molecularly imprinted polymer, enzyme immunoassay, 1H-NMR, and infrared spectroscopy have been used to identify and evaluate chemical constituents in Panax species. Moreover, Soxhlet extraction, heat reflux extraction, ultrasonic extraction, solid phase extraction, microwave-assisted extraction, pressurized liquid extraction, enzyme-assisted extraction, acceleration solvent extraction, matrix solid phase dispersion extraction, and pulsed electric field are discussed. In this review, a total of 219 articles published from 1980 to 2018 are investigated. Panax species including P. notoginseng, P. quinquefolius, sand P. ginseng in the raw and processed forms from different parts, geographical origins, and growing times are studied. Furthermore, the potential biomarkers are screened through the previous articles. It is expected that the review can provide a fundamental for further studies.

• 대한한의학회지
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• 제32권4호
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• pp.48-67
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• 2011

Objectives: There has been a lot of controversy about whether Korean traditional medicines cause drug-induced hepatotoxicity or not. However, this controversy has not been examined from a scientific approach, especially in terms of toxicology. Thus, this article for traditional Korean medicine-induced hepatotoxicity was reviewed on a basis of toxicological assessment. Methods: Toxicological assessments were carried out in a view of reactive intermediates formation from biotransformation in animals based on cytochrome P450 activity inducing oxidative stress, and case reports in world-wide. Results and Conclusions: Several herbal materials used for traditional Korean medicines were identified as possible agents causing drug-induced hepatotoxicity. Most of these agents seem to produce toxic reactive intermediates caused by cytochrome P450. Oxidative stress followed by cytochrome P450 activation could also be a reason for herb-induced hepatotoxicity. Plausible prevention of herb-induced hepatotoxicity was suggested.

• 한국의사학회지
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• 제33권1호
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• pp.99-112
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• 2020

In this study, we examine the changes to Korean medicine that occurred when 'proprietary medicines' (賣藥) swept through the pharmaceutical market during the Japanese occupation (1910-1945 C.E.). Proprietary medicine during the Japanese colonial period took various forms including ready-made, over-the-counter, patent, and nostrum type pharmaceuticals. This paper examines how Korean medicine, which was the dominant form of medicine during the Joseon Dynasty, was forced to adapt to the rise of proprietary medicines. We found that the prescription of Korean medicine herbal decoctions became more like proprietary medicine in the way that they were formulated. In addition, prescriptions in Korean medicine books were reformulated with prescriptions and medicines from outside the tradition. Proprietary medicines, many of which were made with secret recipes handed down in a family, also attracted attention. Such prescriptions were made famous through advertisements and further influenced future Korean medicine doctors. New prescriptions took advantage of the trust and authority existing in traditional Korean medicine by introducing ginseng and traditional medicinal herbs such as deer antler velvet (鹿茸, Cervi Parvum Cornu). This paper argues that proprietary medicine of the Japanese colonial period distorted the concept of traditional herbal medicine.

• Advances in Traditional Medicine
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• 제1권1호
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• pp.1-7
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• 2000

Although Oriental medicines have long been used effectively in treating many diseases throughout the world, the pharmacological mechanisms of most Oriental medicines used have not been defined. As part of our continuing search for biologically active antiallergic drugs from natural sources, Oriental medicines were analyzed. Some Oriental medicines have been used against various allergic diseases for generations, and still occupies an important place in traditional medicine in Korea. It is also still unclear how Oriental medicine prevents allergic disease in experimental animal models. Some Korean folk medicines inhibited the mast cell-mediated allergic reaction. This review summarizes the effective folk medicine in experimental effect of allergic reaction. Potential antiallergic folk medicines include: Poncirus trifoliata; Siegesbeckia glabrescence; Solanum lyratum; Aquilaria agallocha; Ulmi radicis; Polygonum tinctorium; Hwanglyun-Haedok-Tang; Rehmannia glutinosa; Kum- Hwag-San; Syzygium aromaticm; Spirulina platensis; Sosiho-Tang; Sinomenium acutum; Schizonepta tenuifolia; Shini-San; Magnoliae flos; Sochungryoung-Tang; Oryza sativa; Cryptotympana atrata; Salviae radix; Rosa davurica; Asiasari radix; Chung-Dae-San; and Cichorium intybus. Understanding the mechanisms of action for these Oriental medicines can permit drug development and laying of the ground-work for evaluating potential synergistic effects by addition and subtraction of prescriptions.

• 생약학회지
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• 제42권4호
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• pp.371-378
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• 2011

Aldose reductase (AR) has been implicated in the development of the diabetic complications. To discover novel treatments for diabetic complications from natural sources, 65 Korean herbal medicines have been investigated for inhibitory activities on AR. Of these, 23 herbal medicines exhibited a significant inhibitory activity compared with 3,3-tetramethyleneglutaric acid (TMG). Particularly, 8 herbal medicines, Acer tataricum (twig, stem and leaf), Acer tataricum (fruit), Rhododendron schlippenbachii (twig, stem and leaf), Weigela subsessilis (twig, stem and leaf), Acer mono (branch and leaf), Ailanthus altissima (twig, stem and leaf), Lindera obtusiloba (branch and leaf), Solidago serotina (whole plant) showed three times more potent inhibitory activity than the positive control, TMG.