• Title/Summary/Keyword: trace analysis

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Anti-Ionizing Radiation Effect of Selenium on Osteoporosis Model during Bone Repair Process (골다공증 모델의 뼈 재생기에 있어 셀레늄(Selenium)의 방사선 보호작용)

  • Kim, Hyun;Cho, Sang-Rae;Choe, Changyong;Seong, Hwan-Hoo
    • Journal of Embryo Transfer
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    • v.30 no.3
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    • pp.201-206
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    • 2015
  • Selenium (Se) is an essential trace element for humans and animals, and several findings suggest that dietary Se intake may be necessary for bone health. Accumulating evidence indicates that Se compounds possess anticancer properties. Se is specifically incorporated into proteins in the form of selenocysteine and non-specifically incorporated as selenomethionine in place of methionine. This study evaluated protection by Se in the bone repair process in ovariectomized rats after irradiation. For such purpose, 80 ovariectomized female Sprague-Dawley rats were randomly divided into 4 experimental groups: ovariectomized (Ov), Ov/Se, Ov/irradiated (Irr) and Ov/ Se/Irr. A bone defect was created on the tibia of all animals 40 days after ovariectomy. Two days after surgery, only the Ov/Se and Ov/Se/Irr rats received 0.8 mg Se/kg. Three days after surgery, only the Ov/Irr and Ov/Se/Irr rats received 10 Gy of X-rays on the lower limb region. The animals were euthanized at 7, 15, 22 and 29 days after surgery to assess the repair process, which was evaluated by analysis of trabecular bone number (Masson Trichrome) and birefringence analysis (Picrosirius). It was possible to observe a delay in the bone repair process in the ovariectomized/irradiated group and similarity between the ovariectomized, Ov/ Se and Ov/Se/Irr groups. Our findings suggest that sodium selenite may influence a radioprotective effect in the bone repair of tibia of ovariectomized rats without toxicity.

Analysis of Synthetic Fragrances (SFs) in Water Using Stir Bar Sorptive Extraction (SBSE) and GC-MS/MS (교반막대 추출법과 GC-MS/MS를 이용한 수중의 합성 향물질류 분석)

  • Seo, Chang-Dong;Son, Hee-Jong;Yoom, Hoon-Sik;Choi, Jin-Taek;Ryu, Dong-Choon;Kwon, Ki-Won;Jang, Seung-Ho
    • Journal of Korean Society of Environmental Engineers
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    • v.36 no.6
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    • pp.387-395
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    • 2014
  • A highly sensitive analytical method based on stir bar sorptive extraction (SBSE) technique and gas chromatography/tandem mass spectrometry (GC-MS/MS) has been developed, allowing the simultaneous multi-analyte determination of 11 synthetic fragrances (SFs) in water samples. The stir bar coated with polydimethylsiloxane (PDMS) was added to 40 mL of water sample at pH 3 and stirred at 1,100 rpm for 120 min. Other SBSE parameters (salt effect and presence of organic solvent) were optimised. The method shows good linearity (coefficients > 0.990) and reproducibility (RSD < 10.9%). The extraction efficiencies were above 83% for all the compounds. The limits of detections (LOD) and limits of quantification (LOQ) were 2.1~4.1 ng/L and 6.6~12.9 ng/L, respectively. The developed method offers the ability to detect 11 SFs at ultra-low concentration levels with only 40 mL of sample volume. Matrix effects in tap water, river water, wastewater treatment plant (WWTP) final effluent water and seawater were investigated and it was shown that the method is suitable for the analysis of trace level of 11 SFs. The method developed in the present study has the advantage of being rapid, simple, high-sensitive and both user and environmentally friendly.

Longitudinal Analysis of Information Science Research in JASIST 1985-2009 (정보학연구의 25년간 동향 분석 : JASIST 논문을 중심으로)

  • Seo, Eun-Gyoung
    • Journal of the Korean Society for information Management
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    • v.27 no.2
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    • pp.129-155
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    • 2010
  • In recent years, the changes in information technology have been so dramatic and the rate of changes has increased so much that information science research rigorously evolves with the passage of time and proliferates in diverging research directions dynamically. The aims of this study are to provide a global overview of research trends in information science and to trace its changes in the main topics over time. The study examined the topics of research articles published in JASIST between 1985 and 2009 and identified its changes during five 5 year periods. The study found that the most productive area has consistently been 'Information Retrieval', followed by 'Informetrics', 'Information Use and Users', 'Network and Technology', and 'Publishing and Services'. Information retrieval is a predominant core area in Information Science covering computer-based handling of multimedia information, employment of new semantic methods from other disciplines, and mass information handling on virtual environments. Currently Informetric studies shift from finding existing phenomena to seeking valuable descriptive results and researchers of information use have concentrated especially on information-seeking aspects, so adding greater sophistication to the relatively simple approach taken in information retrieval.

Development of dimethyl sulfide gas CRM and stability test (다이메틸설파이드 가스 인증표준물질 개발 및 안정성 평가)

  • Kim, Young-Doo;Heo, Gwi-Suk;Oh, Sang-Hyub;Kim, Byoung-Moon;Bae, Hyun-Kil;Woo, Jin-Chun
    • Analytical Science and Technology
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    • v.18 no.6
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    • pp.552-558
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    • 2005
  • A type of dimethyl sulfide gas CRM in the ppb level was developed for the analysis of trace-level odorous gas in environmental atmosphere. The concentration of dimethyl sulfide ($(CH_3)_2S$) was 10 umol/mol level in the cylinder filled with nitrogen, 1500 psi. And the variability of the concentration for 3 years was about 0.1% due to the adsorption or instability of $(CH_3)_2S$. The gas standards produced simultaneously in 4 bottles and examined by GC-FID were shown with 0.4%, reproducibility of preparation and 0.25%, standard uncertainty due to weighing and purity. The relative expended uncertainty of 1.1% (95% of confidence level, k=2) was assigned to the certified value of 10 umol/mol level of $(CH_3)_2S$ after quantitative evaluation on the purity, mixing, weighing, analysis, adsorption and stability of dimethyl sulfide gas.

Modelling of the noise-added saturated steam table using neural networks (노이즈가 포함된 포화증기표의 신경회로망 모델링)

  • Lee, Tae-Hwan;Park, Jin-Hyun
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.15 no.2
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    • pp.413-418
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    • 2011
  • The thermodynamic properties of steam table are obtained by measurement or approximate calculation under appropriate assumptions. Therefore they are supposed to have basic measurement errors. And thermodynamic properties should be modeled through function approximation for using in numerical analysis. In order to make noised thermodynamic properties corresponding to measurement errors, random numbers are generated, adjusted to appropriate magnitudes and added to original thermodynamic properties. Both neural networks and quadratic spline interpolation method are introduced for function approximation of these modified thermodynamic properties in the saturated water based on pressure and temperature. In analysis spline interpolation method gives much less relative errors than neural networks at both ends of data. Excluding the both ends of data, the relative errors of neural networks is generally within ${\pm}0.2%$ and those of spline interpolation method within ${\pm}0.5$~1.5%. This means that the neural networks give smaller relative errors compared with quadratic spline interpolation method within range of use. From this fact it was confirmed that the neural networks trace the original values better than the quadratic interpolation method and neural networks are more appropriate method in modelling the saturated steam table.

Isolation and Identification of Zinc-Enriched Yeast Saccharomyces cerevisiae FF-10 from the Tropical Fruit Rambutan (열대과일 Rambutan으로부터 아연 고함유 효모 Saccharomyces cerevisiae FF-10 분리 및 특성)

  • Cha, Jae-Young;Heo, Jin-Sun;Kim, Jung-Wook;Lee, Seon-Woo;Cho, Young-Su
    • Journal of Life Science
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    • v.18 no.4
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    • pp.447-453
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    • 2008
  • Zinc is an essential trace element in Human Being. Highly zinc containing yeast strain isolated from the tropical fruit, rambutan and the zinc concentration in this yeast strain was 306 ppm (30.6 mg%)per dry matter basis. This strain was found to be a rounded type, normal size, and multi-polar budding. Phylogenetic analysis using the ITS1-5.85 rDNA sequences from isolated strain is most similar to yeast Saccharomyces cerevisiae at the level of nucleotide sequence identity at 99%. This strain was produced alcohol by about 12% using fully colonized koji-rice with Aspergillus oryzae. In conclusion, the isolated strain was found to be closely related to the S. cerevisiae based on its morphological and physiological properties, and alcohol fermentation. The phylogenetic analysis of strain FF-10 using ITS 5.8S rDNA sequence data also supported the closely related to the S. cerevisiae. Accordingly, the isolated yeast was named as S. cerevisiae FF-10. Further studies on the best culture conditions for zinc production from zinc-enriched S. cerevisiae FF-10 are under investigation.

Bronze Production Technology in the Early Iron Age: A comparative study of bronze artifacts recovered from the Hoam-dong site in Chungju and Chongsong-ri in Buyeo (초기철기시대 청동기의 제작기술 - 충주 호암동유적과 부여 청송리유적 출토 청동기의 비교 연구-)

  • Han, Woorim;Hwang, Jinju;Kim, Sojin
    • Korean Journal of Heritage: History & Science
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    • v.51 no.4
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    • pp.224-233
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    • 2018
  • Thirty-three Early Iron Age bronzes at the sites of Hoam-dong in Chungju and Cheongsong-ri in Buyeo were investigated in order to study the manufacturing technique and the provenance of lead. Chemical analysis using X-ray fluorescence showed that 33 bronzes consist of copper(Cu), tin(Sn) and lead(Pb) served as major elements. Major and minor elemental analyses by EPMA were performed on two mirrors and 2 weapons of the bronzes investigated. The results shows that bronze mirrors from Chungju and Buyeo were high-tin bronzes(> 30 wt%). And 20% of tin and 5% of lead were founded in bronze weapons. Iron, zinc, arsenic, silver, nickel, sulfur and cobalt detected in four bronzes as minor and trace elements. The four bronzes were alloyed considering their function and were not heat treated after casting due to their high tin content. Lead isotope analysis using TIMS indicates that thirty-three bronzes were distributed southern Korea peninsula except Zone 1. As a result, lead raw materials came from various regions in Korean Peninsula not from Gyeongsang-do regions. The manufacturing techniques of bronze ware generalized at this age, and bronze was produced in various sites using raw materials from various sources.

Establishment of Accuracy Criteria of Flood Inundation Map Using Quantitative Evaluation Indices (정량적 평가 지표를 활용한 호우피해 예측지도의 정확도 판단기준 설정)

  • Lee, Jin-Young;Kim, Dongkyun;Park, Kyung Woon;Kim, Tae-Woong
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.39 no.3
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    • pp.381-389
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    • 2019
  • It is important to estimate flood overflow because adverse weather phenomena are frequently occurring in recent years. In order to cope with such abnormal floods, it is essential to perform flood inundation simulations for constructing flood inundation maps as nonstructural countermeasures. However, there is no quantitative evaluation method and criterion for flood inundation prediction. In this study, the Receiver Operation Characteristics (ROC) and Lee Sallee Shape Index (LSSI) were employed to quantitatively evaluate the accuracy of flood inundation maps for 10 administrative districts. Comparing predicted inundation maps with actual inundation trace maps, the ROC score was 0.631 and the LSSI was 25.16 %. Using the ROC and the LSSI, we proposed an evaluation criterion for flood inundation map. The average score was set as an intermediate score and distributed into 5 intervals. The validity of the evaluation criterion was investigated by applying to the XP-SWMM model, which has been verified and corrected. The ROC analysis result was 0.8496 and the LSSI was 51.92 %. It is considered that the proposed evaluation criteria can be applied to flood inundation maps.

Comparative Analysis of Nutritional Components of Protaetia brevitarsis Larvae Fed Soybean Curd Cake (비지박 함유 먹이원 급이 흰점박이꽃무지 유충의 영양성분 비교분석)

  • Kim, Sun Young;Yoo, Taeho;Ji, Sangmin;Song, Jeong-Hun;Kim, So-Yun;Chang, Gyu-Dong
    • Journal of Life Science
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    • v.32 no.12
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    • pp.997-1004
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    • 2022
  • To verify the nutritional superiority and safety of Protaetia brevitarsis larvae fed a food source containing soybean curd cake, the nutrients and harmful substances of larvae reared only with fermented oak sawdust and a food source containing soybean curd cake were comparatively analyzed. The crude protein content was 50.0% in the larvae fed with a food source containing soybean curd cake (SCC), which was 1.1 times higher than that of the larvae fed with fermented oak sawdust (FOS) at 44.7%. Potassium, which showed the highest content among macrominerals, was 2.5 times higher in SCC (3,169.6 mg/100 g) than in FOS (1,245.9 mg/100 g). Among trace minerals, zinc showed 12.6 times higher content than FOS (8.2 mg/100 g) in SCC (103.0 mg/100 g). As a result of the analysis of harmful substances, all heavy metals met the edible insect control standards, and pathogenic microorganisms, such as E. coli and Salmonella spp, were not detected. According to the above study, the larvae of P. brevitarsis fed soybean curd cake contain various nutrients, and safety has also been confirmed, so it is judged to be suitable for use as food.

Column Comparison for the Separation of Ferimzone Z and E Stereoisomers and Development of Trace Residue Analysis Method in Brown Rice Using HPLC-MS/MS (컬럼 비교를 통한 Ferimzone Z 및 E 입체 이성질체의 물질 분리 및 HPLC-MS/MS를 활용한 현미 중 미량잔류분석법 개발)

  • Mun-Ju Jeong;So-Hee Kim;Hye-Ran Eun;Ye-Jin Lee;Su-Min Kim;Jae-Woon Baek;Yoon-Hee Lee;Yongho Shin
    • Korean Journal of Environmental Agriculture
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    • v.42 no.3
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    • pp.203-210
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    • 2023
  • Ferimzone Z is a fungicide for effectively controlling rice blast. Under light irradiation conditions, it undergoes a rapid conversion to its E-stereoisomer. Given the importance of isomers in risk assessments of residues in crops, an analytical method was developed for individual isomer quantification. A comparative analysis performed using two columns in HPLC-MS/MS demonstrated that the isomers were successfully separated using the Cadenza column. For the brown rice sample preparation, 5 g of the homogenized sample was saturated with 7 mL of water. The sample was then extracted with a 10 mL mixed solvent of acetonitrile and ethyl acetate (1:1, v/v) that contained 0.1% formic acid, and it was subsequently partitioned with magnesium sulfate and sodium chloride. The upper layer was purified using dSPE containing C18 and PSA sorbents. The established method was subjected to method validation, and it showed recovery rates of 90.6-98.8% (RSD ≤ 3.9%) at concentrations of 0.01, 0.1, 2 mg/kg, with a soft matrix effect (%ME) ranging from -3.1% to +6.5%. This method can be employed in monitoring studies of brown rice to determine the conversion ratio from the Z isomers to the E isomers.