• Journal of Microbiology and Biotechnology
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• v.26 no.9
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• pp.1613-1619
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• 2016

Francisella tularensis is a highly virulent pathogen of humans and other mammals. Moreover, F. tularensis has been designated a category A biothreat agent, and there is growing interest in the development of a protective vaccine. In the present study, we determine the in vitro and in vivo immune responses of a subunit vaccine composed of recombinant peptides Tul4 and FopA from epitopes of the F. tularensis outer membrane proteins. The recombinant peptides with adjuvant CpG induced robust immunophenotypic change of dendritic cell (DC) maturation and secretion of inflammatory cytokines (IL-6, IL-12). In addition, the matured DCs enabled ex vivo proliferation of naive splenocytes in a mixed lymphocyte reaction. Lastly, we determined the in vivo immune response by assessment of antibody production in C57BL/6 mice. Total IgG levels were produced after immunization and peaked in 6 weeks, and moreover, Tul4-specific IgG was confirmed in the mice receiving peptides with or without CpG. Based on these results, we concluded that the recombinant peptides Tul4 and FopA have immunogenicity and could be a safe subunit vaccine candidate approach against F. tularensis.

• The Korean Journal of Health Service Management
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• v.7 no.3
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• pp.83-93
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• 2013

This study was conducted on 350 students of health and medical department of universities in Busan from June 3, 2013 to June 21, 2013, and a total of 350 questionnaires were researched. Among them 337 responses were used after 13 unfaithful responses were excluded. The effects of oral health belief on use of dental service of students of health and medical department of universities were examined and analyzed to provide basic data which can be used to expand understanding about oral health belief, to promote changes in behaviors regarding oral health and to improve oral health of the public. Collected data was analyzed through SPSS (Statistical Packages for Social Science 15.0. SPSS Inc. USA). For verification of differences of oral health belief depending on general matters and oral health behavior, t-test and ANOVA analysis were conducted, and for examination of the effects of oral health belief on use of medical service, logistic regression and regression analysis were conducted. The study results suggest that those who had higher sensitivity among oral health belief variables had higher probability of needing dental treatment and seeing the dentist immediately. And those who showed higher sensitivity and importance had higher frequency of seeing the dentist for six months. Future studies need to be conducted on the methods to change oral health belief for sustainable and systematic oral health enhancement in consideration of the factors affecting oral health belief and oral health behavior of the students of dental hygiene department.

• Asian-Australasian Journal of Animal Sciences
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• v.20 no.6
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• pp.971-975
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• 2007

Two experiments were conducted to evaluate the feeding values of red pepper seed oil meal (RPSOM) and to investigate its dietary supplementation on broiler performances. In Exp. 1, nutritional values of RPSOM were evaluated by analyzing chemical composition and determining true metabolizable energy (TME), nitrogen corrected TME (TMEn) and true available amino acid (TAAA). RPSOM contained 22.50% of CP, 4.75% of ether extract, 27.70% of crude fiber, 4.73% of crude ash and 49.97 ppm of xanthophylls. The contents of capsaicin and dihydrocapsaicin were 34 mg and 31 mg/100 g DM, respectively. The values of TME and TMEn determined by force-feeding 16 roosters were 1.73 kcal/g and 1.63 kcal/g DM, respectively. The average TAAA value of 17 amino acids was 85.22%. In Exp. 2, a total of 225 Ross male broiler chicks, 3 weeks old, were randomly divided into 9 groups of 25 birds each and assigned to three experimental diets containing 0 (control), 5 or 10% RPSOM fed ad libitum for 3 weeks. No significant differences were observed in growth performances and carcass characteristics. The level of serum cholesterol in the 10% RPSOM group was significantly lower than that of the control group (113.92 vs. 137.50 mg/dl). The dietary RPSOM at 5 and 10% levels increased the content of C18:2 ${\omega}{\sigma}$ in leg muscle compared with the control group. The results suggested that RPSOM can be included into broiler feed up to 10% without any negative effects on broiler performances and physiological responses and used as a non-conventional plant protein source, if its nutritional values are well evaluated.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.6
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• pp.2587-2592
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• 2012

Background: Limited human papillomavirus (HPV) related knowledge might be a barrier to future vaccine acceptance. From 2008-2010, PATH conducted an HPV vaccination demonstration project in partnership with the government immunization program in Vietnam, which included awareness campaigns prior to vaccination. Objective: To assess and compare knowledge and attitudes about cervical cancer and HPV vaccines between mothers and daughters, and whether knowledge was associated with vaccination status. Methods: We analyzed HPV-related knowledge and attitude data from mother-daughter paired responses to a cross-sectional household survey. After parents completed the survey, daughters were asked the same questions. We calculated the frequency of responses for each question and devised a scaled composite measure for knowledge. Results: Participants believed they had received enough information about cervical cancer and HPV vaccines and it was sufficient to make a decision about vaccination. Fifty percent of the participants knew HPV causes cervical cancer and 80% knew the HPV vaccine prevented cervical cancer. Mothers had more knowledge about cervical cancer and HPV infection (p<0.01), compared to daughters, who had more vaccine specific knowledge (p<0.01). However, the total mean knowledge score was similar for the groups. Girls not fully vaccinated had a lower mean knowledge score than fully vaccinated girls (p<0.001). Conclusions: Our results suggest that the purpose of the HPV vaccine was clearly messaged; however, some misconceptions about cervical cancer and HPV still exist. Limited knowledge about the magnitude of cervical cancer, HPV as a cause of cervical cancer, and HPV vaccines may have contributed to incomplete vaccination.

• Journal of the Korean Magnetics Society
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• v.1 no.2
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• pp.31-36
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• 1991

Structural and compositional heterogeneities of sputter deposited, amorphous $Co_{87}Zr_{4}NB_{9}$ thin films were investigated using TEM and EDS with windowless detector. The films deposited with substrate bias and annealed in rotating magnetc field showed two amorphous phases of Co-rich region and (ZrNb)oxide-rich region, and revealed 'ultra-soft' magnetic properties. Revesible bias-responses and overdamped frequency responses, along with small Hc, Hk and Mr/Ms ratio, give the possibility of ultra-soft magnetic behavior fo CoZrNb thin films. We proposed the vortex type magnetization distribution in remanent state which was correlated with the thin film heterogeneity. Then, the ultra-soft characteristics of the compositionally heterogeneous films were explained by the spin vortices that minimized the total magnetostatic and exchange coupling energies.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.9 no.3
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• pp.20-29
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• 2010

The variable message sign(VMS) is a representative traffic information service medium which has the largest users and is provided and operated by a governmental agency. In this paper, we focused on psychological responses of individuals who use VMS traffic information in the light of the need for VMS to provide user-oriented service. A total of t재 scenarios were plotted to conduct our experiment on a virtual test track. A stress index analysis on VMS location, the first scenario, showed the lowest stress value at the point of 1.5 kilometers before the exit. A stress index analysis on the contents of VMS information (communication, time, and speed), the second scenario, showed the lowest stress value at time information.

• Journal of Life Science
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• v.20 no.8
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• pp.1173-1180
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• 2010

We investigated changes in photosynthesis and activity of ascorbate peroxidase (APX) that scavenges ROS as responses to oxidative stress induced by salinity in rice (Oryza sativa L.). Photosynthetic efficiency of rice leaves, monitored in terms of Fv/Fm, declined with the increase of salt concentration (100-300 mM NaCl). Salinity caused an increase of $H_2O_2$ in leaves of rice, with an increase of APX activity. Among total APX isoforms, an isoform of stromal-APX 1 in leaves of rice was completely inactivated by 300 mM NaCl, but was not affected by chilling or drought. The results suggest that salt stress acts in quite a different mechanism in relation to the activity of stromal-APX from that of other stresses such chilling and drought. We carried out RT-PCR for analysis of genes expression of APX isoforms as affected by salt stress. The expression of cytosolic APX/thylakoid-bound APX genes in leaves of rice exposed to salt stress was increased, while stromal APX gene expression rapidly declined.

• Journal of Korean Elementary Science Education
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• v.26 no.2
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• pp.216-225
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• 2007

The purpose of this study is to find out the differences (if any) and the causes of preferences for science between elementary school boys and girls. Another purpose of this study is to find out when such differences in preference begin and what their causes we. Fourth, fifth and sixth grades from elementary school A in Gyeongg-gi Province participated twice in research surveys on preference levels in science with education, career course and personal experience taken into account. A total of 997 survey responses, (excluding no responses or half-hearted ones) were selected for comparative analysis. The analysis methods used were frequency analysis, cross stabs and one-way ANOVA Analysis which depended on survey items. The results of this study show that preference levels in science are 61.7% and the difference of preference levels in science between boys and girls are 31.2% and 30.5% with boys showing higher percentages in science preference levels than girls(p<.01). Moreover, the results also show that the point in which changes in preference level in science emerges is from fifth and sixth grades. Therefore, the gender difference in preference levels in science begins with fifth grade students, with science being slightly more favored by boys. finally, the main causes in gender differences seems to be the experiment participants' levels, scientific education textbook preference level, interests in science class, and plans for the future that are related to field of science. Among these causes, interests level in science class and preference levels in science are closely related.

• Culinary science and hospitality research
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• v.23 no.5
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• pp.58-66
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• 2017

The purpose of this study was to assess priority of educational needs for the development of curriculum based on key competencies of NCS for departments related to culinary arts in a 2-year college. In order to achieve this objective, the survey has been distributed to cooks working in hotel restaurants or restaurant companies by social network service and a total of 360 responses were analyzed excluding 18 responses. The collected surveys were analysed by using paired t-test, Borich's needs analysis and the locus for focus analysis. The results of this study were as follows. First, the difference between the level of importance and present level about key competencies recognized by cooks working in restaurant company was statistically significant in 9 fields of key competencies excluding mathematical competence. The difference recognized by cooks working in hotel was statistically significant in all 10 fields of key competencies. Second, the results of Borich's needs assessment showed that the education needs of problem solving competence were the highest, followed by interpersonal, resource management, communication, information competence. Finally, considering both Borich's needs assessment and the locus for focus analysis, cooks working in hotel restaurants recognized that educational needs of communication, interpersonal, problem-solving, information competence were the top priority, and cooks working in restaurant companies recognized that educational needs of communication, interpersonal, skill, resource management competence were the top priority. Therefore, it is necessary to develop curriculum for culinary arts specialists based on key competence of top priority.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.25 no.3
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• pp.101-118
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• 2014

Given the context of rapid developing ubiquitous computing environment, it is imperative for users to search and use images based on affective meanings. However, it has been difficult to index affective meanings of image since emotions of image are substantially subjective and highly abstract. In addition, utilizing low level features of image for indexing affective meanings of image has been limited for high level concepts of image. To facilitate the access points of affective meanings of image, this study aims to utilize user-provided responses of images. For a data set, emotional words are collected and cleaned from twenty participants with a set of fifteen images, three images for each of basic emotions, love, sad, fear, anger, and happy. A total of 399 unique emotion words are revealed and 1,093 times appeared in this data set. Through co-word analysis and network analysis of emotional words from users' responses, this study demonstrates expanded word sets for five basic emotions. The expanded word sets are characterized with adjective expression and action/behavior expression.