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Comparative Analysis of Immunosuppressive Metabolites Synthesized by an Entomopathogenic Bacterium, Photorhabdus temperata ssp. temperata, to Select Economic Bacterial Culture Media (곤충병원세균(Photorhabdus temperata ssp. temperata) 유래 곤충 면역 억제물질 생성 비교 연구를 통한 저렴한 세균 배지 선발)

  • Seo, Sam-Yeol;Jang, Ho-Jin;Kim, Kun-Woo;Kim, Yong-Gyun
    • Korean journal of applied entomology
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    • v.49 no.4
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    • pp.409-416
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    • 2010
  • An entomopathogenic bacterium, Photorhabdus temperata ssp. temperata (Ptt), suppresses insect immune responses and facilitates its symbiotic nematode development in target insects. The immunosuppressive activity of Ptt enhances pathogenicity of various microbial pesticides including Bacillus thuringiensis (Bt). This study was performed to select a cheap and efficient bacterial culture medium for large scale culturing of the bacteria. Relatively cheap industrial bacterial culture media (MY and M2) were compared to two research media, Luria-Bertani (LB) and tryptic soy broth (TSB). In all tested media, a constant initial population of Ptt multiplied and reached a stationary phase at 48 h. However, more bacterial colony densities were detected in LB and TSB at the stationary phase compared to two industrial media. All bacterial culture broth gave significant synergism to Bt pathogenicity against third instars of the diamondback moth, Plutella xylostella. Production of bacterial metabolites extracted by either hexane or ethyl acetate did not show any significant difference in total mass among four culture media. Reverse phase HPLC separated the four bacterial metabolites, which were not much different in quantities among four bacterial culture broths. This study suggests that two industrial bacterial culture media can be used to economically culture Ptt in a large scale.

Study on job satisfaction and organizational commitment between regular and irregular workers of Distribution Companies (유통업체 정규직 / 비정규직의 직무만족과 조직몰입에 관한 연구)

  • Lee, Hee-Phil;Kim, Hong
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.3 no.3
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    • pp.89-123
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    • 2008
  • This study has the purpose to research on the actual conditions of discriminative treatment between regular and irregular work and find what kind of environment factors can effect on the job-satisfaction, organizational commitments. Especially, this study has addressed to improvement of conditions for irregular work and researched the difference factors between regular and irregular workers's job-satisfaction, organizational commitment, and employment - environmental. For the data collection, a total of 400 questionnaires were distributed for survey, 195 responses were available for statistical analysis. This questionnaires were sent to the distribution companies that has a variety type of employment as regular and irregular worker in Seoul province by e-mail and direct way. And SPSS 12.0(ver.) was used to analyze the data. The main results were as the following; First, the human relationship and the normative commitment were statistically different between regular and irregular work in the part of job satisfaction and organizational commitment. Second, income were important to improve the satisfaction of job environment and personnel policy and the normative commitment. Corporation welfare were important for the satisfaction of human relationship, job environment and personnel policy, normative commitment affective commitment and continuous commitment. Stability of employment were important to improve the satisfaction of human relationship and job environment, normative commitment and affective commitment.

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EFFECTS OF DIFFERENT OXIDIZED SURFACES OF IMPLANT ON OSSEOINTEGRATION; RESONANCE FREQUENCY AND HISTOMORPHOMETRIC ANALYSIS STUDY IN MINI-PIGS (다양한 양극산화막 처리방법이 임플란트 골유착에 미치는 영향)

  • Kwon, Kyung-Hwan;Min, Seung-Ki;Sung, Dae-Hyuk;Choi, Jae-Young;Choi, Seung-Lim;Cha, Su-Ryeon;Oh, Hee-Kyun
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.29 no.5
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    • pp.383-393
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    • 2007
  • The aim of the present study is to investigate the effect of anodized surface of osseointegration implants by using of resonance frequency analysis (RFA) and histomorphometric analysis. A total of 96 screw-shaped implants were devided into 4 groups. Seventy-two implants were prepared by electrochemical oxidation with 3 different ways; Group 1 (n=24) were prepared at galvanostatic mode in 0.25M sulfuric acid and phosphoric acid, Group 2 (n=24) were prepared at galvanostatic mode in calcium glycerophosphate and calcium acetate, and Group 3 (n=24) were prepared at galvanostatic mode in 0.25M sulfuric acid and phosphoric acid followed by Calcium metaphosphate(CMP) coating. Control group (n=24) were the RBM surfaced implants. The implants were placed in the mandibles of 12 mini pigs. Bone tissue responses were evaluated by resonance frequency analysis(RFA) and histomorphometric analysis that were undertaken at 2, 4 and 6weeks after implant placement. The following result were obtained. 1. Twenty-two of 96 implants (4 in control group, 5 in group 1, 7 in group 2, and 6 in group 3) were failed due to faliure of osseoitegration. The failure rate of osseointegration was 22.9%. 2. The mean values of RFA in control, group 2 and groups 3 showed the similar values, but there was no significant difference among groups. 3. Histomorphometric evaluation demonstrated significantly higher bone-to-implant contact ratio in group 2 at 3 and 4 weeks after implant placement than other groups (p<0.05), but there was no significant difference among groups at 6weeks after implant placement.

A STUDY OP THE CURRICULUM IMPROVEMENT OP NURSING EDUCATION (간호교육과정의 개선을 위한 일 조사연구)

  • 이연순
    • Journal of Korean Academy of Nursing
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    • v.4 no.2
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    • pp.67-77
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    • 1974
  • Introduction This study is concerned with improving the nursing education curriculum. The purpose of the study was to 1. Review the basic principles of curriculum development. 2. Determine opinion about current nursing curriculums from recent nursing school graduates. Methods of the study A questionnaire was distributed to 250 nurses, in f general hospitals in Seoul. The nurses were graduated from nursing schools in 1973, but represented all types of nursing programs. The Questionnaire was designed to illicit responses about nursing school curriculums in 5 areas: 1. Establishment of objectives 2. Selection of learning experience 3. Organization of learning experience 4. Instruction 5. Evaluation Summary of the major findings 1. Establishment of objectives Thirty nine point three percent of the nurses said that the first objective of the general nursing education was stated "to educate a nurse as a human being with humanity:" Ninety point eight percent of the total respondents said that thor knew the objectives of the curriculum of their nursing schools, but more half of them (58.3%) understood the objectives vaguely at the time they attended nursing school. 2. Selection of learning experience Thirty nine point eight percent of the respondents said their curriculum contained "only a few" liberal arts courses. 3. Organization of learning experience forty seven point six percent. of the respondents were reported that the number of course hours was "insufficient" in liberal arts courses. Fifty seven point three percent of the nurses felt there was an overemphasis on any special topic in liberal arts courses. 4. Instruction Methods of instruction were as follows: Liberal arts courses-a. lecture which centered on explanation (61.1%), b. lecture which centered on writing (43.7%) Supporting nursing courses-a. lecture which centered on explanation (34.0%). b. laboratory Practice (37.8%) Clinical nursing courses-a. lecture which centered on writing (33.0%), b. clinical practice (38.4%) The percentage of respondents replying that various equipment for the learning activities were "insufficient"were as follows: Liberal arts courses-51.0% Supporting nursing courses-28.6% Clinical nursing courses-37.9% 5. Evaluation In the liberal arts courses, 30.6% o( the respondents answered that the evaluation of records was in their opinion "poor, " and 28.2% of the respondents reported that the method of evaluation was "unfair and discouraging. " "Development of a systematic theory of nursing" was thought to be essential for curriculum improvement. The respondents felt that the junior year was the hardest because 1. Number of subjects was too great (28.1%) 2. Lack of a systematic theory of nursing (14.5%) 3. Conflict due to the difference between nursing theory and nursing practice (21.4%) Recommendations 1. Since this study was limited to nurses who had graduated in 1973, a larger study involving professors, graduate students, and nurses from a wider area is suggested in order to establish the reliability and validity of the present findings. 2. Nursing research should be carried out more actively in order to develope nursing science. 3. A permanent organization for research into ways to improve the curriculum should be established in each nursing school.

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Effects of walking exercise for wellness convergence in the digital age - Based on physical activity - (디지털 시대에 융복합적인 웰니스를 위한 걷기 운동의 효과 - 신체활동 중심으로 -)

  • Kim, Myoung-Su;Kim, Sung-Hee;Lee, Shin-Ho
    • Journal of Digital Convergence
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    • v.13 no.5
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    • pp.365-374
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    • 2015
  • The goal of this study was 1) to compare the physical activity between the normal(NO) and obese (OB) group at baseline and 2) to investigate responses of the physical activity to a 24-week walking exercise program in both groups. A total of 29 middle-aged women was classified NO and OB group based on the BMI($25kg/m^2$). Walking exercise was designed to energy expenditure of 500 kcal per day, with a frequence of 3 days per week for 24 weeks. Body composition(Weight, BMI, BF(%), Hip, WHR) were measured at the baseline and after the walking exercise program(24 week). Physical activity were measured during 24 week for wellness convergence in the digital age. In conclusion, the current findings of this study indicate that 1) physical activity is not difference in both groups at baseline 2) 24-week walking exercise program is an effective means of improving physical activity in both NO and OB groups.

Comparisons of Cognitive Functions between Urban and Rural Dwelling Older Adults: A Preliminary Study (도시와 농촌거주 노인의 인지기능 비교: 예비연구)

  • Kim, Jung Wan;Kang, Yeonwook;Yoon, Ji Hye
    • Journal of Digital Convergence
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    • v.15 no.4
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    • pp.469-476
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    • 2017
  • Mini-Mental State Examination (MMSE) and naming tests have been used clinically as screening and diagnostic tools for mild cognitive impairment and dementia. Researches in other countries have reported that residential areas would affect one's cognitive abilities. In Korean, however, there was no systematic studies on geographical influences to cognitive ability among a sample of urban/rural residents. The aim of this study was to investigate the effect of urban/rural locality of residence on cognitive tests in normal elderly Korean. Residents aged 65 years and older (N=108) were administered the K-MMSE, confrontation/generative naming tests, and medical/socio-demographic interview. When comparing the total number of correct responses between urban residents and rural residents, the performances of rural group were significantly worse than those of the urban group in K-MMSE. Based on the results, our finding suggests that a factor of residential locality should be considered to examine the function and explain the cognitive decline in rural community-dwelling elders.

The Role of Effective Tutors Recognized by Students who have Experienced Problem-Based Learning-Focused on Focus Group Interview (문제중심학습을 경험한 학생들이 인식하는 효과적인 튜터의 역할-Focus group을 중심으로)

  • Choi, Eun-Young
    • The Journal of the Convergence on Culture Technology
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    • v.6 no.4
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    • pp.469-476
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    • 2020
  • This study is a qualitative study to grasp the meaning of the effective role of tutors of college students experiencing problem-based learning through focus group interviews. The subjects of this study were 4th grade students in the Department of Nursing who had experienced PBL, and a total of 21 people were divided into 3 groups and interviewed. Data were analyzed in content to find meaningful topics. The results of this study were analyzed into two subjects and eight categories. Based on the research results, if we summarize the role of effective tutors recognized by students in PBL. 1) Create a permissive atmosphere so that students can express their ideas freely and interact. 2) Give praise and encouragement. 3) Encourage all learners to participate. In particular, learners with low participation should be asked questions to promote active discussion. 4) Avoid unnecessary intervention and participate in the discussion together. 5) Adjust the learning speed and manage the time well. 6) Develop a scenario that meets the goals. 7) Classes are run around questions that can promote learners' thinking. 8) It plays a role of helping to form knowledge by providing positive feedback to learners' responses.

Contents Strategic Approach to Tourism Destination Storytelling Ads (관광스토리텔링 광고효과에 관한 컨텐츠전략적 접근)

  • Lee, Eun-Mi;Uan, ZhulLiy;Yoon, Sung-Joon
    • The Journal of the Convergence on Culture Technology
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    • v.6 no.4
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    • pp.245-252
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    • 2020
  • Recently, as the income level of consumers increases, interest in tourism products has increased, competition among tourist destinations has been fierce. Storytelling ads are drawing attracting attention as one of the ways to establish differentiated positioning for tourism products. This study investigates the role of the involvement of tourism activity in the storytelling ads effects, and examines the effects of the attitude toward storytelling ads on tourist destination commitments, visit intention and SNS WOM (word-of-mouth) intention. This study also empirically tests the role of mental simulation in the relationship between storytelling ads attitudes and visit intention. A total of 255 usable responses were obtained from a sample of 270 consumers aged 18 or older in South Korea. Following the two-stage approach of model validation, measurement validity of each construct appearing in the structural model and hypothesized structural paths were tested. The findings show that all hypotheses are supported except H3 that posits the impact of attitude toward the storytelling ads on visit intention and mental simulation showed a moderate effect on the relationship between ads attitude and visit intention in the case of rational appeal. This study suggests that the production of storytelling ads of tourist destinations can be effective strategy to improve regional competitiveness and increase visit and SNS WOM intention.

THE DEGREE OF EGO IMPAIRMENT IN ADOLESCENT PSYCHIATRIC PATIENT GROUPS (청소년 정신과 환자군을 대상으로 한 자아기능의 장애정도 비교 - Rorschach검사의 자아손상지표를 중심으로 -)

  • Oh, Youn-Hee;Kim, Zoung-Soul;Shin, Min-Sup
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • v.10 no.2
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    • pp.178-185
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    • 1999
  • Objective:This study was designed to examine the validity of the Ego Impairment Index(EII) in adolescent psychiatric patients(depression, depressive-conduct disorder, schizophrenia), and to explore the validity of the depressive-conduct disorder as a diagnostic entity. Method:19 depressives, 13 depressive-conduct disordered, and 10 schizophrenics with the age from 12 to 18 were selected based on DSM-IV and ICD-10 criteria, and their responses of the Rorschach and MMPI were analyzed. The EII was empirically developed by Perry and Viglione(1991) in order to assess the degree of ego impairment, and was derived from the Rorschach test. Results:It was founded that a single factor, ego impairment, was derived from the principal component analysis and explained 57.18% of total variances. The degree of ego impairment was grater in schizophrenics than in the other two groups, but there were no difference between depressives and depressive-conducts. Conclusions:These results offered support for the use EII as an empirical means of assessing the degree of ego impairment in adolescent patients. And these results suggest that depressive-conduct disorder group is more similar to the depressive group, implying that depressive-conduct disorder might correspond to so called masked depression. The clinical implication and limitation of present study were discussed.

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Mediating Effects of Perceived Risk and Usefulness between Online Information Credibility and Intention to Use (온라인 정보의 신뢰성 및 정보 활용의도 사이의 지각된 위험과 유용성의 매개효과에 관한 연구)

  • Sun, Jonghak;Yoon, Jung-Hyeon
    • Management & Information Systems Review
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    • v.33 no.4
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    • pp.99-118
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    • 2014
  • Using the theory of attribution, this study investigates the determinants if controllability and explores underlying dimensions of online information credibility, and then investigates if the credibility of online information influences the users' intention to use the online information for evaluating or judging the involved products. Moreover, as a research attempt to investigate the impacts of online information credibility, this study examines whether the main effect of perceived online information credibility on the intention of using online information to make a decision of purchase is mediated by both perceived risk and perceived usefulness. A total of 287 survey forms were collected from online consumers. We examined reliability by exploring internal consistency of the multiple item scales in the overall sample. Convergent and discriminant validity were also examined for evidence of construct validity. Then, PLS technique was employed to test the research model. As a result of analyzing data from a dataset of 287 responses via PLS technique, it is found that (1) both sources (controllability and stability) of perceived credibility are significantly associated with both perceived risk and perceived usefulness, and (2) perceived risk as well as perceived usefulness partially mediate the link between the two sources of credibility and intention to use. The findings of this study also suggest that the two dimensions of online information credibility influence information recipient's intention to use. Moreover, the online information including descriptions about controllability and stability can trigger potential consumers to perceive risk about consumption of the informed products and services. Therefore, providing online information with highly described controllability and stability can increase not only the credibility of the online information itself, but also the intention to use the online information through perceived risk and usefulness.

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