• Title/Summary/Keyword: total reflection

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Development of a Total Internal Reflection Fluorescence (TIRF) Microscopy for Precise Imaging the Drying Pattern of a Sessile Droplet (고착 액적 증발면의 정밀 관측을 위한 전반사 형광 현미경 기법 개발)

  • Wonho Cho;Jinkee Lee
    • Journal of the Korean Society of Visualization
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    • v.21 no.3
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    • pp.65-74
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    • 2023
  • Compared to epifluorescence(EPI) microscopy which captures fluorescence from the entire depth of sample, total internal reflection fluorescence(TIRF) can selectively visualize only a single surface of it. TIRF uses a thin evanescent field generated by the total internal reflection of laser light on surface. However, conventional TIRF system are designed for total internal reflection to occur at the upper surface of sample, making them unsuitable for sessile droplet imaging. We designed a TIRF system suitable for a sessile droplet imaging by utilizing slide glass as a lightguide. We presented the details for constructing the TIRF system using a prism, slide glass, air slit, and optical trap. Then, we compared the TIRF with EPI by imaging the droplet with fluorescent particles during its drying process. As a result, TIRF allows us to distinctly visualize the drying pattern on the bottom surface of droplet.

Characteristics of Nanoscale Modes Guided by the Total External Reflection of Surface Plasmon-Polaritons (표면 플라즈몬-폴라리톤의 외부-전반사에 의해 도파되는 나노 크기 모드의 특성)

  • Seol, Kang Hee;Song, Seok Ho
    • Korean Journal of Optics and Photonics
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    • v.23 no.1
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    • pp.36-41
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    • 2012
  • Total external reflection (TER), which does not occur on a dielectric interface, is a unique feature of surface plasmon-polaritons (SPP). We propose an SPP-TER waveguide structure consisting of low-index dielectric nanocore covered with high-index dielectric on a flat metal surface. The SPP mode confined in the nanocore by the TER effect has a mode size much smaller than wavelength scale. Numerical comparison of mode characteristics between the SPP-TER waveguides and other total-internal-reflection-based waveguides such as metal or high-index dielectric nanowires show that the SPP-TER structures can possess higher modal gain for applications of nanocavity lasers.

Secondary Optics Design of Dissymmetrical Light Distribution for 100 W LED Safety Luminaires (100 W급 LED 보안 등기구용 비대칭 배광의 2차 렌즈 설계)

  • Shin, Ik-Tae;Yang, Jong-Kyung;Lee, Dong-Jin;Par, Dae-Hee
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.23 no.6
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    • pp.471-476
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    • 2010
  • This paper has studied dissymmetrical light distribution design using the secondary optics in the simulation. fundamental Lambertian radiation distribution based on LED has been cut off by the secondary optics, and the first surface slope of lens and second surface slope of total reflection area have been calculated through formula. PMMA (the index of refraction: 1.49361) which is material of lens has been selected. critical angle($42.02993^{\circ}$) between Air and PMMA has been calulated by snell's law, and total reflection angle slope has been selected about $16.67^{\circ}$ to occur the total reflection. when the first surface slope and the second surface slope has been set up, Rays of all total reflection area have generated the total reflection. finally, designed LED Module has been estimated by Korean Industrial Standards for LED safety street lighting. dissymmetrical light distribution have been analyzed with reached effect of road illuminance, and average road illuminance which are each 70.6 lx, 40.35 lx, and 25.88 lx have been satisfied with Korean Industrial Standards for LED safety street lighting.

Fluorescece Microscope using Total Internal Reflection for Measuring Biochip (내부 전반사 방식에 의한 바이오칩 측정 장비)

  • Bae, Soo-Jin;Kang, Uk
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.56 no.9
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    • pp.1694-1698
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    • 2007
  • This study suggests a new fluorescence microscope to observe micro-samples within fluorophore in a variety of biomedical fields including the fluorescence analysis of a biochip, such as a DNA micro-array. A fluorescence microscope is a device for irradiating light onto a micro-object, executing an excitation and fluorescence emission process. In this study, it adopts a total internal reflection fluorescence(TIRF) method to excite a whole micro-sample substrate different from an existing way which uses an evanescent wave resulting from a total internal reflection on the micro-sample surface. Suggested TIRF microscope can reduce optical noise and obtain images with higher sensitivity thus obtain precise information about the density, quantity, location, etc. of a flurophore, and can simultaneously process separate images even when plurality of fluorophores having different excitation and fluorescent wavelength ranges is distributed, thus easily obtain information about the fluorophores.

TM polarized photon tunneling in a frustrated total internal reflection structure (Frustrated Total Internal Reflection 구조에서의 TM 편광된 광자의 터널링)

  • Lee, Byoung-Ho;Lee, Wook
    • Korean Journal of Optics and Photonics
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    • v.7 no.2
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    • pp.112-119
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    • 1996
  • TM polarized photon tunneling time and the position where TM photons appear after two-dimensional tunneling in a frustrated total internal reflection (FTIR) structure are calculated. This is done by converting the FTIR tunneling problem of TM photons to an electron tunneling problem with a position-dependent effective mass for a heterostructure potential barrier. Derived results are compared with TE photon cases.

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Analysis of Students' Reflective Journals on Medical Communication Role Plays (의료면담 역할극 사례의 학습자 성찰일지 분석)

  • Kim, Young Jon;Lee, Seung Hyun;Yoo, Hyo Hyun
    • Korean Medical Education Review
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    • v.19 no.3
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    • pp.169-174
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    • 2017
  • This study explores the phenomenon of learner reflection in writing. Sixty-nine reflection logs were submitted by students after medical interview role plays. Using thematic analysis methodology, the following three steps were carried out: (1) the entire contents of the logs were analyzed and coded, (2) the coded contents were reclassified and categorized into higher themes, and (3) all reflection logs were re-analyzed to derive the frequency according to their classifications. The learners' reflection contents were classified into 'preparation,' 'experience,' and 'future plan.' The levels of reflection were 'simple information and appreciation,' 'acquisition and application of knowledge,' and 'analysis and criticism.' The types of reflection were identified only at the level of 'analysis and criticism' and could be classified into 'relativization,' 'objectification,' and 'internalization.' Students' reflection levels were highest in the levels of 'acquisition and application of knowledge' (49.3%), and 'analysis and criticism' (37.7%). A total of 14.5% of the students showed 'internalization.' In order to enable internalized reflection at a critical level, instructional strategies to induce and promote reflection are needed.

Polymer $1{\times}2$ Thermo-Optic Digital Optical Switch Based on the Total-Internal-Reflection Effect

  • Han, Young-Tak;Shin, Jang-Uk;Park, Sang-Ho;Han, Sang-Pil;Baek, Yong-Soon;Lee, Chul-Hee;Noh, Young-Ouk;Park, Hyo-Hoon
    • ETRI Journal
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    • v.33 no.2
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    • pp.275-278
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    • 2011
  • This letter presents a polymer $1{\times}2$ thermo-optic totalinternal-reflection digital optical switch (TIR-DOS) with an index contrast of 1.5%-${\delta}$ operating at low power consumption. The structure of our $1{\times}2$ TIR-DOS was created by adding a reflection port to that of a conventional multimode filtering variable optical attenuator. To improve the total-internalr-eflection efficiency, a heater offset was applied to the crossing region of multimode waveguides of the TIR-DOS. The fabricated $1{\times}2$ TIR-DOS shows a low electrical power consumption of 18 mW for an on-off ratio of 35 dB.

An Analysis of Electric-field Density into Mountain Area Using DTED (디지털 지도를 이용한 산악지형의 전계강도 분석)

  • Lim, Joong-Soo;Chae, Gyoo-Soo;Park, Young-Chul;Kim, Min-Nyum
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.7 no.5
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    • pp.852-857
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    • 2006
  • This paper presents a precision method to calculate the electric field density of mountain area using digital terrain elevation data(DTED). Generally we calculate the electric field density of a point adding a direct field density and horizontal reflection field density between two points. In this paper, we consider a vertical reflection field density from vertical surface near the wave propagation line between transmitter and receiver. The vertical reflection electric field have different propagation path and polarization from a horizontal reflection field. And the total electric field density adding horizontal field density and vertical reflection value is more accurate than a direct path electrical field density or direct field density adding a horizontal reflection field density.

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The Relationship between Welding Conditions and Ultrasonic Reflection Coefficients of Dissimilar Metals Friction Welded Joints (이종재 막찰용접 이음부에서의 초음파 반사계수와 용접조건과의 관계)

  • 오세규;김동조;한상덕
    • Journal of Ocean Engineering and Technology
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    • v.1 no.1
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    • pp.138-143
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    • 1987
  • Friction welding has emerged as a reliable process for high-production commercial applications with significant economic and technical advantages. But nondestructive test in friction weld was not clearly developed. Therefore the experimental verification is necessary in order to understand the characteristcs of the pulse echo effects according to various change in welding conditions. This paper presents an attempt to determine the relationship between the varios welding conditions and the coefficients of reflection using the ultrasonic pulse echo method in dissibilar metals friction weld. The new approach of calculating the coefficients of reflection based on measured amplitudes of the echoes is applied in this paper. These coefficients provides a single quantitative measurement which involves both acoustic energy reflected at the welded interface as well as transmitted across the interface. As a result, it was known that the quantitave relationship between welding conditions and the coefficients of reflection using the ultrasonic pulse echo exists in dissimilar metals friction weld.

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