• Food Science and Biotechnology
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• 제18권6호
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• pp.1464-1469
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• 2009

Jiwhang (Rehmannia glutinosa), one of the most widely used medicinal herbs, was dried with various methods such as sun drying, hot air drying, vacuum drying, and freeze drying methods, and their effects on the antioxidant capacity in relation with the content of total phenolic compounds were studied with a steamed-and-dried rehmannia (sookjiwhang) for comparison. Generally, total phenolic contents decreased significantly by all of the drying treatments except the steamed-and-dried rehmannia, in which total phenolic contents increased 2.4 fold compared with fresh rehmannia. Content of verbascoside, a functional phenolic compound, was the highest in the freeze-dried rehmannia ($177.97{\pm}0.02\;{\mu}g/g$ d.m.) followed by vacuum-dried ($105.55{\pm}0.07\;{\mu}g/g$ d.m.), hot air-dried ($23.01{\pm}0.02\;{\mu}g/g$ d.m.), and sun-dried ($4.89{\pm}0.13\;{\mu}g/g$ d.m.) ones comparable to the fresh rehmannia ($80.15{\pm}1.26\;{\mu}g/g$ d.m.). Antioxidant capacity determined by both 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis (3-ethyl-benzthiazoline-6-sulphonic acid) (ABTS) methods agreed with the result of total phenolic contents, that is, the antioxidant capacity was the highest in the steamed-and-dried rehmannia followed by fresh rehmannia, vacuum-dried, hot air-dried, sun-dried, and freeze-dried ones. Conclusively, the total phenolic contents and antioxidant capacity of rehmannia were greatly affected by the drying methods used.

• 한국작물학회지
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• 제51권spc1호
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• pp.209-214
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• 2006

전국의 산과 들의 생육조건이 다양한 지역에서 생육한 약쑥, 뺑쑥, 참쑥, 제비쑥, 물쑥, 황해쑥, 더위지기, 가는잎쑥 등에 대하여 수집한 쑥 100계통을 실험에 이용하여 항산화력과 항산화 성분 함량을 비교 분석하였다. 합성 항산화제인 BHT와 비교 실험한 지질과산화 억제력을 살펴보았을때 모든 쑥 시료에서 BHT 200 ppm과 유사한 억제력을 나타내어 그 우수성을 확인할 수 있었다. 쑥 수집종 100계통의 AEAC, EDA, 총페놀함량, 플라보노이드 등을 분석한 결과 쑥 수집종의 총 페놀화합물 함량은 $156{\sim}1,767mg/100g$ 범위로 최대 10배의 함량변이를 보였는데, 총 페놀화합물의 함량이 900mg/100g 이상인 수집종은 총 20 개체였다. 플라보노이드 함량도 종간, 수집지역 간에 편차가 컸는데 $82.9{\sim}852.2mg/100g$ 범위로 변이의 폭이 컸다. 전자공여능의 경우 $13.4{\sim}95.0%$의 범위로 다양하였으며 전자공여능이 90% 이상인 수집종은 총 23개체였다. ABTS와 DPPH 라디칼을 이용한 에탄을 추출물의 항산화력 측정 결과 총 페놀화합물 함량이 높았던 대부분의 시료에서 높은 라디칼 제거능을 나타내었다. 본 연구 결과 항산화력이 우수하며 생육 특성 및 수량성이 우수한 AC-60, AC-67, AC-77을 우수 계통으로 선발하였다.

• 생약학회지
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• 제48권3호
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• pp.208-212
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• 2017

In this study, analysis of active compounds that are believed to be highly relevant to antioxidant activity was carried out on the methanol extract and its solvent fractions of Corni fructus. The DPPH radical scavenging activity for the comparison of antioxidant activity was higher in order of aqueous fraction > methanol extract > ethyl acetate fraction > n-hexane fraction. It is similar to the order of total polyphenol contents in the samples. As a result of LC-MS analysis, phenolic acid compounds such as caffeic acid, gallic acid and chlorogenic acid and lognin, which is known as a representative active ingredient of Corni fructus, were identified as active compounds. And the antioxidative activity and the total polyphenol content of the extracts and solvent fractions were found to be related to the contents of the compounds. Particularly, it was confirmed that phenolic acid such as caffeic acid contributes to the antioxidative activity of the aqueous fraction of Corni fructus methanol extract.

• 한국자원식물학회지
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• 제12권3호
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• pp.215-220
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• 1999

전남지방의 고로쇠나무 수액에 대해 sucrose, 무기물, phenol화합물을 분석한 결과는 다음과 같다. 1. Sucrose의 함량은 일반적으로 2차 채취시기에 높게 나타났으며, 지역에 따라 차이는 있으나 2∼3%정도였다. 채취방법은 사구법이 천공법보다 높게 나타났다. 2. ToTal phenol화합물 역시 2차 채취시기에 높게 나타났으며, 0.034∼0.151 mg/100ml의 수준이었고, 가장 다량 함유된 phenol화합물은 phloroglucinol이었으며, 역시 사구법에서 천공법보다 높게 나타났다. 3.무기물의 경우 역시 2차 채취시기에 높게 나타났으며, 역시 사구법에서 천공법보다 높게 나타났다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.64-64
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• 2019

Screening and identification of genetic resources based on their phytoconstituents and morphological characters potentially provide baseline data for researchers, breeders, and nutraceutical companies who wish to formulate a nutrient-dense diet and health beneficial supplement. Thus, we evaluated the amount of total phenolic content and major phenolic compounds; examined if phenolic compounds could be used as distinguishing factors for perilla genetic resources; and investigated the relation between some quantitative and qualitative morphological characters with the contents of phenolic compounds in 360 accessions obtained from National Agrobiodiversity Center gene bank, Jeonju, Korea. Total phenolic content (TPC) was estimated using Folin-Ciocalteu colorimetric assay. Individual phenolic compounds were determined using an Ultra-High Performance Liquid Chromatography system equipped with Photodiode Array detector. Considerable variations were observed in TPC (7.99 to 117.47 mg GAE/g DE), rosmarinic acid (RA) (ND to 19.19 mg/g DE), caffeic acid (CA) (ND to 0.72 mg/g DE), apigenin-7-O-diglucuronide (ADG) (ND to 1.24 mg luteolin equivalent (LUE)/g DE), scutellarein-7-O-glucuronide (SG) (ND to 4.32 mg LUE/g DE), and apigenin-7-O-glucuronide (AG) (ND to 1.60 mg LUE/g DE). RA was the most dominant phenolic compound in most accessions (95.3%) followed by SG. The adaxial leaf color was light green, green and dark green in 13.8%, 65.0%, and 21.1 % of the accessions, respectively. 78.8% of the accessions had light green color at the abaxial side with the remaining being described as green. Most of the accessions (96.9%) were cordate shape, the remaining being eclipse. Intensities of green pigment at abaxial and adaxial leaf surfaces were correlated with contents of individual phenolic compounds and TPC whereas leaf length and width had no correlation with TPC, CA and RA, and negatively correlated with ADG, AG, and SG. Leaf shape was not related with content of phenolic compounds, color of leaves, or the length or width of leaves. Accessions IT57426, IT157434, IT267710, and IT267712 which contained relatively high contents of TPC and major phenolic compounds (RA and SG) could be used for further research in breeding and bioassay test. Our study result showed the contents of total phenolics and individual phenolic compounds along with the morphological characters could be useful distinguishing factors for perilla genetic resources.

• 한국약용작물학회지
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• 제20권6호
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• pp.447-453
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• 2012

This study was conducted to determine the contents of phenolic compounds and astragalosides in Astragalus membranaceus. Between the phenolic compound groups, flavonoids compounds (51.13%) had higher average concentrations than the phenolic acids groups. Among the 30 phenolic compounds, quercetin ($353.11{\mu}g\;g^{-1}$) provided the highest concentrations. According to different cultivation year, 3-year-cultivated Astragalus ($2612.57{\mu}g\;g^{-1}$) showed the highest concentrations of phenolic compounds. According to different harvest days, 6/5 harvesting Astragalus ($2615.80{\mu}g\;g^{-1}$) showed the highest concentrations of phenolic acids. Comparison between the top and subterranean parts of harvested Astragalus plants cultivated for 2 years, 3 years, and 5 years showed big difference of total phenolic compounds in concentrations. Further, the top part had higher amounts of the total phenolic compounds than the subterranean part among all Astragalus. This tendency was similar to those of the top root and lateral root. The concentration of the phenolic compounds in the top root was higher than that of the lateral root.

• 한국식품영양과학회지
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• 제46권8호
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• pp.937-943
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• 2017

본 연구에서는 발아에 의한 팥의 기능성분 변화를 살펴보기 위하여 6일 동안 발아시키면서 유리형 및 결합형 폴리페놀, 플라보노이드, 페놀산 및 안토시아닌의 변화를 분석하였다. 유리형 폴리페놀 및 플라보노이드 함량은 발아기간이 증가함에 따라 각각 약 2.6배 및 5배 정도 증가하였으며, 결합형 폴리페놀 및 플라보노이드 함량은 발아 초기 감소하였다가 발아 3일 및 2일 이후 각각 증가하였다. 발아기간에 따른 구성 페놀산의 변화의 경우 ferulic acid, veratric acid, hesperidin, salicylic acid, naringenin 및 hesperidin과 총 페놀산은 유리형 및 결합형 페놀산 함량이 모두 증가하였지만, 안토시아닌 및 안토시아니딘은 침지 및 발아과정에서 손실되어 감소하였다. 본 연구 결과 팥을 발아시킬 경우 폴리페놀, 플라보노이드 및 페놀산 함량이 증가함에 따라 팥에 함유된 기능성분의 이용성을 증대시키기 위해서는 발아공정의 적용이 효과적이라고 판단된다.

• 한방비만학회지
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• 제23권1호
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• pp.10-17
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• 2023

Objectives: The aim of this study was to compare the total phenolic content of Korean Acanthopanax senticosus stems harvested in different seasons and provide basic data for developing functional reinforcement products based on the optimal harvesting time. Methods: Each sample harvested in different seasons was extracted and concentrated twice for 2 hours using 70% ethyl alcohol. Phenolic compounds were analyzed using high-performance liquid chromatography for simultaneous multi-component analysis of 14 compounds, including syringaresinol and so on. Results: The results showed that the stem of Korean Acanthopanax senticosus harvested in winter (November 29th) (EAS-5) had the highest phenolic content of 1.038%. The stem of Korean Acanthopanax senticosus harvested in autumn (October 1st) (EAS-4) showed the second-highest phenolic content of 0.764%, followed by the stem of Korean Acanthopanax senticosus harvested in spring (February 2nd) (EAS-1) with a content of 0.390%. On the other hand, the stem of Korean Acanthopanax senticosus harvested in the summer (June 2nd) (EAS-3) showed the lowest content at 0.342%. In conclusion, the stem of Korean Acanthopanax senticosus harvested in winter (EAS-5) showed the highest phenolic compound content. Conclusions: Considering the extraction yield and the total phenolic content, as well as the concentrations of key functional components such as eleutheroside B, chlorogenic acid, and syringaresinol in the 70% ethyl alcohol extract of Korean Acanthopanax senticosus, it is suggested that the stems of Korean Acanthopanax senticosus harvested during the winter season are suitable for the development of novel materials with enhanced anti-obesity functionality.

• 한국식품조리과학회지
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• 제29권3호
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• pp.275-281
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• 2013

This study was performed to evaluate the quality characteristics and antioxidative activities of cookies prepared with different amounts of purple sweet potato powder. Moisture and crude ash contents were lowest in the control group followed in order by the 10%, 20%, and 30% purple sweet potato powder addition groups. The lightness and yellowness values measured by Hunter color system decreased based on the amount of purple sweet potato powder concentration added to cookies. The total phenolic compound and anthocyanin contents were the highest in 30% purple sweet potato powder added cookies. The DPPH radical scavenging activity of 30% purple sweet potato powder added cookies showed the highest values among groups. In the sensory evaluation, the scores for taste, texture and overall preference were significantly highest in cookies with 10% and 20% purple sweet potato powder. Considering the scores of antioxidant activity and sensory evaluation, the concentration of between 10% and 20% addition of purple sweet potato powder would be ideal for development of functional cookies.

• 한국식품조리과학회지
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• 제28권6호
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• pp.773-779
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• 2012

This study was conducted to investigate the physiological activities of saccharified cherry tomato gruel containing different levels(0, 15, 30, and 45%) of cherry tomato puree. The total phenolic compound and flavonoids contents of saccharified cherry tomato gruel with cherry tomato puree were 1.73-5.09 mg/g and 0.28-7.01 mg/g, respectively. DPPH radical scavenging activities of saccharified cherry tomato gruel with cherry tomato puree were 8.67-92.58%, respectively. The ABTS radical scavenging activities of saccharified cherry tomato gruel with cherry tomato puree ranged from 6.02 to 61.59%. The ACE inhibitory activity of cherry tomato rice gruel increased with cherry tomato puree showed a range of 38.85-62.15%, respectively. With increasing additions of cherry tomato puree, the total phenolic compound and flavonoid contents, DPPH and ABTS radical scavenging activities and, ACE inhibitory activities were increased significantly (p<0.05). In conclusion, saccharified cherry tomato gruel could be used as a gruel processing method to in order to increase the functional values of gruels.