• 원예과학기술지
• /
• 제29권5호
• /
• pp.467-473
• /
• 2011

Flavonoid are large group of the polyphenolic compounds which are distinguished by an aromatic or phenolic ring structure and the phenolic compounds are induced by microbial infection, ultraviolet radiation, temperature and chemical stress. They are known for their antioxidant activity, anti-allergic, anti-inflammatory, anti-microbial and anti-cancer activities. In this study, changes in flavonoid content were investigated using heterologous chalcone isomerase (CHI) expression system. Also, phenolic compounds level was measured to examine the relation between flavonoids and phenols contents. Explants of lettuce (Lactuca sativa L.) were transformed with Agrobacterium tumefaciens LBA 4404 strain containing pFLH-CHI (derived from pPZP2Ha3) vector constructed with CHI gene from Brassica rapa. The putative transgenic plants were confirmed by genomic DNA PCR analysis. Also the transcription levels of the gene were analyzed by semi-quantitative RT-PCR with gene specific primers. The total flavonoid contents were increased at $T_0$ and $T_1$ generations over 1.4 and 4.0 fold, respectively. Total phenol contents also increased at $T_1$ generation. These results indicate that CHI gene plays an important role to regulate the accumulation of flavonoids and its component changes.

• 한국자원식물학회지
• /
• 제30권6호
• /
• pp.686-692
• /
• 2017

The aim of this study was to determine the suitability of surfactant to extract higher phenolic compound, flavonoid and antioxidant activity from Tartary buckwheat and evaluate the potentiality of surfactant as a screening agent for breeding purpose. Primarily, we employed two types of surfactant (Hydrophilic: Tween 20 and Lipophilic: Span 80) to select the suitable surfactant agent for the extraction of optimum bioactive compounds. Between two surfactants, Tween 20 showed highest efficiency at 4 mM concentration to extract total phenolic content (TP), total flavonoid (TF) and antioxidant activity (AA). Tween 20 at 4 mM concentration was fixed for further analysis along with hot water ($90^{\circ}C$) treatment as a control. In our findings, highest TP (118 mg/g), TF (38 mg/g) and AA (76%) was achieved in KW21 and KW22 among the fifteen accessions of Tartary buckwheat. In other way, TP, TF and AA was 200%, 120% and 110% higher in surfactant formulation compared with control treatment, respectively.

• 한국유기농업학회지
• /
• 제26권3호
• /
• pp.477-488
• /
• 2018

본 연구에서는 국내 주요재배 매실 품종의 총 페놀, 플라보노이드 함량과 항산화 효과를 확인하고자 하였다. 총 페놀 함량은 $64.13-93.43mg{\cdot}100g^{-1}$으로 품종 간 함량에 차이가 있었다. 이 중 '앵숙'과 '단아'의 함량이 비교적 높게 나타났다. 또한 총 플라보노이드 함량을 측정한 결과 $6.16-18.57mg{\cdot}100g^{-1}$으로 총 페놀과 같이 품종 간에 유의적인 함량 차이를 보였다. 이 중 '고성'에서 가장 높은 함량을 보였다. DPPH 라디칼 소거활성을 측정한 결과 '남고', '회향실', '장속', '단아', '앵숙'에서 모두 50% 이상 비교적 강하게 나타났다. ABTS 라디칼 소거활성 측정 결과 모든 품종에서 80% 이상으로 전체적으로 높은 소거할성을 보였다. 이 중 '앵숙'이 가장 높은 소거활성을 보였다. 아질산염 소거 능력은 모든 품종에서 50% 이상의 높은 소거능력을 가지고 있는 것으로 확인 되었는데 이중 '남고'(76.03%), '고성'(70.56%), '양로'(70.32%)에서 70% 이상으로 비교적 높은 소거 활성을 보였다.

• Fisheries and Aquatic Sciences
• /
• 제18권2호
• /
• pp.123-130
• /
• 2015

Seaweed Sargassum horneri extracts were obtained using supercritical carbon dioxide ($SC-CO_2$) and different solvents. $SC-CO_2$ was kept at a temperature of $45^{\circ}C$ and pressure of 250 bar. The flow rate of $CO_2$ (27 g/min) was constant during the entire 2-h extraction period, and ethanol was used as a cosolvent. Six different solvents [acetone, hexane, methanol, ethanol, acetone mix methanol (7:3), and hexane mix ethanol (9:1)] were used for extraction and agitated by magnetic stirring (250 rpm) in the dark at $25^{\circ}C$ for 20 h; the ratio of material to solvent was 1:10 (w/v). Antioxidant properties of S. horneri extracted using $SC-CO_2$ with ethanol and different solvents have shown good activity. The highest activity belongs to $SC-CO_2$ with ethanol extracted oil, showing DPPH, ABTS, total phenolic content, and total flavonoid levels of $68.38{\pm}1.21%$, $83.51{\pm}1.25%$, $0.64{\pm}0.02mg/g$, and $5.57{\pm}0.05mg/g$, respectively. The S. horneri extracts showed a significant correlation between the antioxidant activity and phenolic content. Based on these results, the $SC-CO_2$ extract (ethanol) of the seaweed extract from brown seaweed may be a valuable antioxidant source.

• 한국의류학회지
• /
• 제42권2호
• /
• pp.269-277
• /
• 2018

This study extracted Artemisia capillaris Thunberg with distilled water and ethanol to investigate its antioxidant effect. We then investigated the possibility of producing nanofibers by an electrospinning process by adding the extracts to polyvinyl alcohol (PVA). The electrospinning method used a PVA concentration of 12wt.%, an applied voltage of 10kV, and a tip-to-collector distance of 15cm. Total polyphenol and total flavonoid contents were measured to verify the antioxidant activity of Artemisia capillaris Thunberg extracts (ACEs). The total polyphenol content of the distilled water extract and the ethanol extract were measured as 218.47 and 271.26mg/g, respectively, and the total flavonoid content of the distilled water extract and the ethanol extract were measured as 141.68 and 34.98mg/g, respectively. As the content of the ACEs in the PVA nanofibers increased, the Diameters of the nanofibers and the uniformity of the diameters decreased. The electrospinning process was fabricated in a relatively uniform form without beads, and the diameters of the nanofibers that were produced ranged from 340 to 390nm. The results of FT-IR, XRD and DSC analyses confirmed that the ACEs were well mixed with the PVA molecules and were electrospun.

• 한국식품영양학회지
• /
• 제35권2호
• /
• pp.96-105
• /
• 2022

This study compared and analyzed the antioxidant effect of Doenjang prepared from vegetable water, and explored the optimal addition ratio of vegetables of 5 kinds and the possibility of application to Doenjang. The sample is three kinds of vegetable water (VW1, VW2, VW3) prepared by adding different ratios of radish, carrot, green onion, onion and shiitake mushroom and Denjang prepared using it. Doenjang was aged and fermented at about 40℃ for 40 days, and then separated and used only solids. The content of their antioxidant compounds was measured the content of total phenolic acid contents and total flavonoid contents. In addition, the antioxidant effect was measured by electron donating activity, SOD-like activity, ABTs radical scavenging activity and reducing power. The total phenolic acid contents and total flavonoid contents were high at VW3 and that Doenjang made with VW3. Electron donating activity and SOD-liked activity were high at VW2 and Doenjang made with VW2. ABTs radical scavenging activity was high in Doenjang made of VW3, and Reducing power was high in VW3. Therefore, if Doenjang is prepared with vegetable water prepared by properly mixing 5 types of vegetables, the possibility of developing Doenjang with high antioxidant effect was suggested.

• Journal of the Korean Wood Science and Technology
• /
• 제46권4호
• /
• pp.346-354
• /
• 2018

Kemenyan resin from Styrax sumatrana is a unique non-timber forest product (NTFP) native from Sumatera Island, Indonesia. It possesses a wide range of applications in the pharmaceutical, perfume, and cosmetics industries. In this paper, six kemenyan resin samples were investigated to evaluate their free radical scavenging activity using 1,1-diphenyl-2-picrylhydrazyl (DPPH) reagent. The kemenyan resin samples, which originated from North Tapanuli, Pakpak Bharat, and Humbang Hasundutan, showed high antioxidant activity with $IC_{50}$ < 16 mg/L. The antioxidant activity of common kemenyan resin constituents, i.e., cinnamic acid, ethyl cinnamate, gallic acid, and vanillin was also investigated as positive control, although they exhibited lower antioxidant activity ($IC_{50}$ < 1000 mg/L), except for gallic acid ($IC_{50}$ = 5,23 mg/L). The total phenolic and flavonoid contents (TPC and TFC) for all samples were 44-66 mg gallic acid equivalents (GAE)/g sample and 143-160 mg quarcetin equivalents (QE)/g sample. The results revealed that kemenyan resin has high potency as an antioxidant and could be used as a natural antioxidant resource.

• Natural Product Sciences
• /
• 제19권2호
• /
• pp.186-191
• /
• 2013

Screenings of potential antioxidant activities of Crataegus pinnatifida BUNGE Major. fruits extracted 80% methanol were performed using four antioxidant assays. Significant differences were observed both in total phenolic contents (TPC) and total flavonoid contents (TFC), DPPH radical scavenging activity, nitric oxide scavenging activity, ABTS radical scavenging assay, and reducing power assay. The total polyphenol content and total flavonoid content in the extract were measured to be $224.4{\pm}0.52$ mg GAE/100 g and $12{\pm}0.25$ mg QE/100 g, respectively. When the tested concentration was $500{\mu}g/mL$, DPPH and ABTS radical-scavenging activities of methanolic extracts were 84.15% and 88.8%, respectively. The reducing power and nitric oxide scavenging activity were increased at the manner of dose-dependently. These results suggest that methanolic extracts of Crataegus pinnatifida Bge. fruits possess excellent radical scavenging activities and may serve as a potential source of natural antioxidant.

• 한국식품과학회지
• /
• 제47권4호
• /
• pp.539-543
• /
• 2015

다래 과실(품종명 만수)의 건조 절편을 제조하고자 열풍건조기를 이용하여 35, 50 및 $70^{\circ}C$의 여러 온도에서 생과를 24시간 동안 열풍건조 하였다. 열풍건조 다래의 총 페놀 함량, 총 플라보노이드 함량과 산화방지능은 열처리하지 않은 생과(대조구)보다 감소하였다. ABTS 및 DPPH 라디칼 소거능에 기반하여 측정한 산화방지능과 총 페놀 함량 및 총 플라보노이드 함량과의 상관관계는 $r^2$이 0.9 이상으로 높은 반면에, ORAC 법을 이용한 산화방지능은 상대적으로 낮은 상관관계를 나타내었다. 이러한 실험결과를 바탕으로 건조다래의 제조 시 온도 등 최적 가공 조건을 확립하여, 기능성이 우수한 건조다래를 개발할 필요가 있다. 또한, 고분자 함량이 많은 과피와 함께 과육의 섭취가 가능한 다래를 활용하여 주스, 젤리 등 다양한 제품을 개발할 필요도 있다.

• 생약학회지
• /
• 제45권2호
• /
• pp.141-146
• /
• 2014

In this study, we investigated on antioxidative activities and tyrosinase inhibitory activities of methanol extract and its fractions from roots of Arctium lappa. The total phenolic compound and flavonoid content of the ethylacetate fraction was found to be 818.29 mg/g and 360.59 mg/g as the highest content. In the measurement of DPPH radical scavenging ability and tyrosinase inhibitory activity, the ethylacetate fraction was higher than the other fractions and the extract. In addition, comparative analysis of phenolic compounds by liquid chromatography-mass spectrometry (MS)/MS system under the multiple-reaction monitoring (MRM) with negative-ion electrospray ionization mode. The main phenolic compounds in the extract and fractions of roots from Arctium lappa were cynarin and chlorogenic acid. The main phenolic compound of the ethylacetate fraction was cynarin. n-Butanol fraction had a significantly higher chlorogenic acid content than other samples. In conclusion, DPPH radical scavenging ability and tyrosinase inhibitory activity of the cynarin-riched ethylacetate fraction showed the highest activity.