• Asian-Australasian Journal of Animal Sciences
• /
• v.29 no.9
• /
• pp.1280-1286
• /
• 2016

This study was aimed to evaluate the in vitro effects of medicinal herb extracts (MHEs) on ruminal fermentation characteristics and the inhibition of protozoa to reduce methane production in the rumen. A fistulated Hanwoo was used as a donor of rumen fluid. The MHEs (T1, Veratrum patulum; T2, Iris ensata var. spontanea; T3, Arisaema ringens; T4, Carduus crispus; T5, Pueraria thunbergiana) were added to the in vitro fermentation bottles containing the rumen fluid and medium. Total volatile fatty acid (tVFA), total gas production, gas profiles, and the ruminal microbe communities were measured. The tVFA concentration was increased or decreased as compared to the control, and there was a significant (p<0.05) difference after 24 h incubation. pH and ruminal disappearance of dry matter did not show significant difference. As the in vitro ruminal fermentation progressed, total gas production in added MHEs was increased, while the methane production was decreased compared to the control. In particular, Arisaema ringens extract led to decrease methane production by more than 43%. In addition, the result of real-time polymerase chain reaction indicted that the protozoa population in all added MHEs decreased more than that of the control. In conclusion, the results of this study indicated that MHEs could have properties that decrease ruminal methanogenesis by inhibiting protozoa species and might be promising feed additives for ruminants.

• Food Science and Preservation
• /
• v.10 no.3
• /
• pp.354-359
• /
• 2003

This study was conducted to investigate the fermentation characteristics of kimchi treated with different methods of water extracts of powdered green tea(GT). Four different kimchis, the SB-kimchi(control) which was not treatment of the extracts, SG-kimchi prepared with Chinese cabbage(CC) salted in 1％ GT containing 10％ brine, DG-kimchi prepared with CC dipped in 1％ GT for 30 min after salting, MS-kimchi prepared with the salted CC mixed with 1％ GT containing seasonings. All kimchis was fermented at 10$^{\circ}C$. The pH of treated-kimchis were maintained higher than those of control products during fermentation, but it showed no big difference between each treated groups. Total microbe of SB-kimchi(6.27-9.37 cfu/mL) was higher than those of GT-treated kimchi(5.17-9.20) during fermentation. The ratio of lactic acid bacteria against total microbe was higher than the treated kimchis. Total polyphenol content of kimchi was 52.75 mg％ in DG-kimchi, 47.71 mg％ in MS-kimchi, 44.89 mg％ in SG-kimchi, 30.70 mg％ in SB-kimchi on the 5th days of fermentation. Scores of crispy taste of SG- and DG-kimchi on the 5th days of fermentation was 4.03 and 4.01 points, respectively which was higher than control products. Scores of fishy and hot taste of GT-treated kimchi were lower than those of control products during all fermentation periods.

• Food Science and Preservation
• /
• v.10 no.3
• /
• pp.345-359
• /
• 2003

This study was conducted to investigate the fermentation characteristics of kimchi treated with different methods of water extracts of powdered green tea(GT). Four different kimchis, the SB-kimchi(control) which was not treatment of the extracts, SG-kimchi prepared with Chinese cabbage(CC) salted in 1％ GT containing 10％ brine, DG-kimchi prepared with CC dipped in 1％ GT for 30 min after salting, MS-kimchi prepared with the salted CC mixed with 1％ GT containing seasonings. All kimchis was fermented at 10$^{\circ}C$. The pH of treated-kimchis were maintained higher than those of control products during fermentation, but it showed no big difference between each treated groups. Total microbe of SB-kimchi(6.27-9.37 cfu/mL) was higher than those of GT-treated kimchi(5.17-9.20) during fermentation. The ratio of lactic acid bacteria against total microbe was higher than the treated kimchis. Total polyphenol content of kimchi was 52.75 mg％ in DG-kimchi, 47.71 mg％ in MS-kimchi, 44.89 mg％ in SG-kimchi, 30.70 mg％ in SB-kimchi on the 5th days of fermentation. Scores of crispy taste of SG- and DG-kimchi on the 5th days of fermentation was 4.03 and 4.01 points, respectively which was higher than control products. Scores of fishy and hot taste of GT-treated kimchi were lower than those of control products during all fermentation periods.

• Journal of the Korea Safety Management & Science
• /
• v.11 no.4
• /
• pp.111-118
• /
• 2009

In this study the real situation of apartment house in seoul is reproduced with multi-zone modeling program CONTAM2.4. This model include disinfection system which is consist of dilution, filtration, UVGI(ultra violet germicidal irradiation). It's energy consumption was also analyzed through the linked model of CONTAM and TRNSYS according to the combination of components. The comparison of total energy consumption through energy analysis revealed that adjusting the air change rate of the UVGI air sterilizer to maintain the same indoor microbe removal capability was more advantageous in terms of energy consumption.

• Proceedings of the Korean Environmental Health Society Conference
• /
• 2005.06a
• /
• pp.406-409
• /
• 2005

Since 2003, Korean government has restricted landfill application of organic waste, which shares approximately 56% of total waste sludge from municipal and industrial wastewater treatment plants. In addition, enforcement of the ocean disposal prohibition law is effective from 2005. Organic sludge was composted for the purpose of converting to organic fertilizer. After moisture content was regulated with bulking agents aerobic treatment performed. When composting was conducted, commercial and activated microbe materials, identified from soil were seeded in sewage sludge. Carbon dioxide production was increased sharply after 24 hours. Temperature and pH of compost reached to $66.2^{\circ}C$ and 8. Heavy metals were lower than their regulatory limits, which enable it to utilize as organic fertilizer.

• Journal of Dairy Science and Biotechnology
• /
• v.39 no.3
• /
• pp.113-120
• /
• 2021

Although the number of incidences of illness caused by ingestion of the bacterial pathogen Enterobacter sakazakii (Cronobacter spp.) has dramatically declined, there remains a need for a robust isolation method to recover this microbe from powdered infant formula (PIF). The current method described in the FDA's Bacteriological Analytical Manual requires multiple steps, and 3-4+ days for complete analysis of PIF isolated E. sakazakii (Cronobacter spp.). We describe a bacteriological method including a one-step enrichment followed by plating on chromogenic agar for presumptive identification of E. sakazakii (Cronobacter spp.). Suspected colonies are confirmed by either biochemical analyses, or a Real-Time PCR-based assay. Using this method, E. sakazakii (Cronobacter spp.) in PIF can be isolated and identified within one day (24 hours).

• Korean Journal Plant Pathology
• /
• v.13 no.2
• /
• pp.100-104
• /
• 1997

Influence of the moisture content in soils was examined on population variations of soil microbes, including Cylindrocarpon destructans causing root rot of ginseng, in vivo and under the field condition. Fungal populations decreased in soils treated with various moisture contents in vivo as days after the treatment in creased, but there was not a significant difference in the population among other treatments except 135% moisture content (flooding) at 15 weeks after the treatment. In flooded soils populations of total fungi and C. destructans were reduced to 1/10 and 1/50 of initial populations, respectively. There was, however, a little difference in the population of total bacteria or Actinomycetes between before and at 15 weeks after flooding. On the other hand, population variations of bacteria and Actinomycetes were much greater than those of fungi at different intervals after the moisture treatment. Variations of microbial populations in flooded soils under the field condition were similar to those in vivo. Especially, populations of Fusarium and pectolytic bacteria in flooded soils were reduced to 1/100 of populations in nonflooded soils at 170 days after treatment.

• Food Science and Preservation
• /
• v.13 no.1
• /
• pp.95-101
• /
• 2006

Quality characteristics of Cheongbukjangs with $1\~3\%$ desalted and dried sea tangle powder were investigated after fermentation for 48 hr at $40^{\circ}C$. Addition of sea tangle, irrespective of concentration, did not affect pH and slime content of Cheongbukjang. Color L* and a* values were generally decreased with increasing sea tangle concentration. There were no significant differences in total microbe, amino nitrogen content, and protease and amylase activity between control and Cheongbukjangs with up to $2\%$ sea tangle. However, addition of $3\%$ sea tangle resulted in decreased total microbe, amino nitrogen content, and enzyme activity compared with those of control. Fiber content increased with increasing sea tangle concentration and hardness increased by 1.5 times at $2\%$ sea tangle and by 2.6 times at $3\%$ sea tangle compared with that of control. Results for sensory evaluation revealed that addition of $2\%$ sea tangle was the optimum concentration in view of reducing bitter taste and odor, and improving overall acceptability.

• Food Science of Animal Resources
• /
• v.38 no.4
• /
• pp.749-758
• /
• 2018

The objective of this study was to provide preliminary data for food industry by investigating the distribution of microorganisms in raw materials and sausage examining the effect of heating temperature on sausage quality. Total microbes in sausage ranged 2.21-3.11 Log CFU/g. Bacillus pumilus, B. licheniformis, Staphylococcus saprophyticus, and Enterococcus faecalis were detected on sausage. Total microbes in raw materials was 1.59-7.16 Log CFU/g. Different types of microorganisms were found depending on raw materials, with B. pumilus and B. subtilis were being detected in both raw materials and sausage. Total microbes in sausage after heating was in the range of 1.10-2.22 Log CFU/g, showing the trend of decrease in total microbe with increasing heating temperature, although the decrease was not significant. With increasing heating temperature, pH and hardness were also increased. The yield of sausage manufactured at $85^{\circ}C$ was 95.42% while that manufactured at $65^{\circ}C$ was 96.67%. Therefore, decreasing heating temperature during sausage production might increase yield and save energy without microbiological effect.

• Food Science and Preservation
• /
• v.6 no.2
• /
• pp.188-193
• /
• 1999

This study was carried out to examine the quality of Korean pork regular loins and hams which m exported in Japanese market. The samples were stored at 0${\pm}$1$^{\circ}C$(A, B companies) and 2${\pm}$1$^{\circ}C$(C, D companies). In analysis of Korean pork exported to Japanese market, the pork of A and D companies showed below 10$\^$6/CFU/$\textrm{cm}^2$ of total aerobic bacteria at 40 days storage and panels felt off-flavor after 40 days. Therefore the edible period will be 40 days through the analysis of microbe and sensory test in porks of A and D companies. The porks of B and C companies showed over 10$\^$6/CFU/$\textrm{cm}^2$ of total aerobic bacteria at 50 days storage and panels couldn't feel off-flavor in sensory test after 50 days. Therefore the edible period will be 50 days through the analysis of microbe and sensory test in porks of B and C companies. As the results, shelf-life of A and D companies will be 32 days and B and C companies will be 40 days from edible period by a factor of 0.8. In analysis of stored pork for export in Korean market, the porks of A and B companies showed over 10$\^$6/CFU/$\textrm{cm}^2$ at 40 days storage and C and D companies did over 10$\^$6/CFU/$\textrm{cm}^2$ at 30 days storage in analysis of microbe. The pork of A and D companies showed 10$^4$CFU/$\textrm{cm}^2$ but B and C companies did 10$\^$5/CFU/$\textrm{cm}^2$ at 50 days in coliform count. Therefore shelf-life of the pork of A company was logest and the next were B and C, but shelf-life of the pork of D company was 20 days, therefore it was even shorter than the others.