• 생태와환경
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• 제41권4호
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• pp.472-484
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• 2008

The calibrated Andong Reservoir hydro-dynamic module (PART I) of the 2-dimensional hydrodynamic and water quality model, CE-QUAL-W2 [v3.2], was applied to examine the dynamics of total phosphorus, and chlorophyll $\alpha$ concentration within Andong Reservoir. The modeling effort was supported with the data collected in the field for a five year period. In general, the model achieved a good accuracy throughout the calibration period for both chlorophyll ${\alpha}$ and total phosphorus concentration. The greatest deviation in algal concentration occurred on $10^{th}$ October, starting at the layer just beneath the surface layer and extending up to the depth of 35 m. This deviation is principally attributed to the effect of temperature on the algal growth rate. Also, on the same date, the model over-predicts hypolimnion and epilimnion total phosphorus concentration but under-predicts the high concentrated plume in the metalimnion. The large amount of upwelling of finer suspended solid particles, and re-suspension of the sediments laden with phosphorus, are thought to have caused high concentration in the epilimnion and hypolimnion, respectively. Nevertheless, the model well reproduced the seasonal dynamics of both chlorophyll a and total phosphorus concentration. Also, the model tracked the interflow of high phosphorus concentration plume brought by the turbid discharge during the Asian summer monsoon season. Two different hypothetical discharge scenarios (discharge from epilimnetic, and hypolimnetic layers) were analyzed to understand the response of total phosphorus interflow plume on the basis of differential discharge gate location. The simulated results showed that the hypolimnetic discharge gate operation ($103{\sim}113\;m$) was the most effective reservoir structural control method in quickly discharging the total phosphorus plume (decrease of in-reservoir concentration by 219% than present level).

• 환경영향평가
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• 제23권3호
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• pp.220-226
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• 2014

This study investigated temporal-spatial and variations in Geostationary Ocean Color Imager (GOCI) products of parameters total suspended solids (TSS) and chlorophyll-a in the North Marine Areas of East China Sea.GOCI data were collected daily from February 2012 to December 2012. The higher chlorophyll-a values were observed during the investigation period. The relatively large increase in TSS and chlorophyll-a at the sampling stations coupled with typhoon events during the summer rainy period. The abnormal chlorophyll-a concentration was mainly driven by meteorological factors such as typhoon and rainfall in the coastal areas of Jeju and the North Marine Areas of East China Sea. The abnormal high chlorophyll-a concentration at the majority of the coastal stations indicate eutrophication of coastal waters, especially Red tide. The events such as eutrophication and abnormal high chlorophyll-a concentration may potentially influence outbreak of Red Tide, detected with GOCI parameters.

• 한국환경농학회지
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• 제33권4호
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• pp.344-349
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• 2014

BACKGROUND: Purpose of the study was to establish the extinction coefficients of chlorophyll a and b in N,N-dimethylformamide(DMF) and Dimethylsulfoxide(DMSO) solvents and to find out the conditions of optimal extraction temperature and time in citrus leaves. METHODS AND RESULTS: Chlorophyll a and b standards were dissolved in DMF and DMSO. Extinction coefficients of chlorophyll pigments were determined and their contents were quantified using spectrophotometer. Chlorophyllous pigments of citrus(Citrus unshiu Marc. cv. Okitsu wase) leaves were extracted at 25, 40, 60 and $80^{\circ}C$ for 4, 6, 8, 24 and 48 hours to determine the optimal extraction condition. CONCLUSION: The extinction coefficients of chlorophyll a(Chl a) and chlorophyll b(Chl b) of DMF extracts for high extinction wavelength were 663.8 and 647.2 nm. Similarly, the high extinction wavelength of DMSO extracts were 665.8 and 649.0 nm for chl a and b respectively. Chl a, Chl b and total chlorophyll content of DMF extracts were Chl a = $12.10A_{663.8}-2.74A_{647.2}$, Chl b = $21.94A_{647.2}-5.06A_{663.8}$ and total $Chl=19.193A_{647.2}+7.04A_{663.8}$. Similarly, Chl a, Chl b and total Chl of DMSO extracts were Chl a = $14.53A_{665.8}-5.40A_{649.0}$, Chl b = $26.98A_{649.0}-7.11A_{665.8}$ and total $Chl=21.58A_{649.0}+7.43A_{665.8}$. The chlorophyll extracts of DMF and DMSO were very stable in dark. High chlorophyll contents of citrus leaves were found at $80^{\circ}C$ extraction for 6 hours in DMF and at $80^{\circ}C$ extraction for 24 hours in DMSO. However, the chlorophyll content was decreased significantly after 8 hours in DMF extraction while it was remained up to 30 hours in DMSO extraction.

• 동굴
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• 제8호
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• pp.43-54
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• 1998

The plant life of caves is made up of species that can live in total darkness. If any plant in a cave contains chlorophyll, the chlorophyll obviously cannot be energized by light rays from th sun. With a few exceptions noted below, none of the plants in the perpetually dark zone contains chlorophyll. The chief plant life there consists of bacteria, including actinomycetes, and fungi.(omitted)

• 한국수산과학회지
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• 제18권1호
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• pp.63-66
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• 1985

동해와 남해 동부연안에서 1983년 가을 중 식물플랑크톤이 함유한 클로로필-a 양을 세포의 크기 별로 구분하여 조사하였다. 1. 가을철 동해 해수중의 세포의 크기 $300{\mu}m$이하 전체의 식물플랑크톤이 함유한 클로로필-a 양은 $0.202{\sim}1.350mg/m^3$(평균과 표준편차는 $0.597{\pm}0.287\;mg/m^3$)이었다. 표층수중에 포함된 양이 심층수 50m층까지)에서의 양에 비해 약간 많았다. 그리고, 수표면 $1m^2$하 수주의 클로로필-a 양은 St.에 따라 큰 차이 없이 평균 $17.013\;mg/m^2$이었다. 2. 세포의 크기 $60{\mu}m$이하의 미세플랑크톤이 함유한 클로로필-a 양은 $0.100{\sim}0.855\;mg/m^3$(평균 및 표준편차는 $0.372{\pm}0.201\;mg/m^3$)로서 표층수에서의 양이 심층수에 비해 약간 많았다. 이를 미세플랑크톤이 전체 식물플랑크톤 현존량에 차지하는 율은 클로로필-a 양으로 $25.4{\sim}83.3\%$로써 평균 $62.2\%$이었고 이 율 역시 표층수에서 약간 높았다.

• Journal of Plant Biology
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• 제26권2호
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• pp.91-99
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• 1983

The formation of chlorophyll-protein complexes (CP-complexes) during the greening of rape cotyledons (Brassica napus cv. Yongdang) was investigated by the SDS-polyacrylamide gel electrophoresis. The total chlorophyll content and Chl a/b ratio were also determined. In addition, the effects of dark treatment on the CP-complex patterns during greening have been examined with respect to their photosynthetic electron transport activity. Greening has brought about the increasein total chlorophyll content and the decrease in Chl a/b ratio, but there have been no changes in Chl a/b ratio after 24 hrs of greening. The light-harvesting chlorophyll a/b-protein complex (LHCP-complex0 was predominant during the initial greening period. Thereafter, the amout of chlorophyll a-protein complex (CP I-complex) was gradually increased. Twenty-four-hr dark treatment immediately after illumination for 6 hrs and 12 hrs resulted in the increase of the Chl a/b ration and the CP I complex, otherwise the decrease of the LHCP-complex. The LHCP/CP I ratio was gradually decreased with further greening, and appeared no change after 48 hrs illumination. The investigation of the photosynthetic electron transport activity indicated that photosystem (PS) II activity (H2Olongrightarrowp-PD*＋FeCy**) did not change, but the activity of PS I was increased suddenly due to the dark treatment. The data suggests that the increase of CP I-complex may result in that of P-700, that is, the increase of PS I activity.

• Journal of Ginseng Research
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• 제21권1호
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• pp.28-34
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• 1997

The effects of light on the pigment production and chloroplast development were examined on ginseng hairy roots cultured in 1/2MS liquid medium. The chlorophyll and carotenoid production were increased from 1,000 to 3,500 lux condition, but decreased drastically in 7,000 lux condition. The anthocyanin production was significantly increased with increment light intensity(1,000∼7,000 lux). The thylakoid membrane of chloroplast was proplastid in dark condition and it began to develop into thylakoid membrane in 1,000 lux condition and then intact thylakoid membrane was developed in 3,500 lux condition. However, the development of thylakoid membrane in 7,000 lux condition was inhibited comparing to 3,500 lux condition. The total chlorophyll production in blue light condition were high comparing to other wavelength and same as 40% of total chlorophyll on white light(3,500 lux) condition. The chlorophyll and carotenoid production by sucrose concentration were high in 3% sucrose condition and anthocyanin production was high in 4% condition. The production of chlorophyll and carotenoid by light periods was high when explants were cultured in dark condition for 1 week and then transferred to light condition for 4 weeks. Our results suggest that pigment production and chloroplast development could be accelerated by light Intensity of specific wavelength in cultures of ginseng hairy root.

• The Korean Journal of Ecology
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• 제18권1호
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• pp.137-146
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• 1995

Acer pseudo-sieboldianum, A. ginnala, A. negundo and A. saccharinum were selected as materials for the studies on the seasonal fluctuation of chlorophyll content and photosynthetic rates. In all the four species during the growing season except in October, the principal component that determined the total chlorophyll content was chlorophyll a. Content of chlorophyll b increased with leaf age, but that of chlorophyll a decreased. In contrast to A. saccharinum and A. pseudo-sieboldianum which showed their maximum chlorophyll content in June, A. negundo, which showed the highest chlorophyll content of the four species, exhibited its maximum chlorophyll content in July, whereas September in the case of A. ginnala. The fluctuation of chlorophyll content was similar to that of air temperature, and it increased till July. But chloprophyll content showed a significant relationship in early stage of leaf development, and there could be any interdependence between them in accordance with the seasonal change, no longer. In all four species light compensation points decreased in accordance with the seasonal change, and the maximum photosynthetic rates were obtained in August. Respiratory rates were shown no significant difference among species, and they decreased according to the adbance of season.

• 한국식품과학회지
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• 제11권3호
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• pp.162-165
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• 1979

신미종(辛味種) 고추인 가락금장 2호를 공시(共試) 품종(品種)으로 하여 추숙(追熟) 시(時)에 red 광(光)을 처리(處理)하여 white 광(光)에 대(對)한 적변(赤變) 효과와 주요(主要) 성분(成分)에 미치는 광질(光質)의 영향(影響)을 조사(調査)하였다. 추숙용(追熟用) 녹숙(綠熟) 고추는 개화(開花) $30{\sim}35$일(日) 정도의 것을 사용(使用)하였고, 이때 실험(實驗)은 $25^{\circ}C$ 항온(恒溫)에서 행(行)하였으며, 약(約) $40\;{\mu}watt/cm^2{\cdot}sec$에너지 수준의 광(光) 처리(處理)를 1일(日) 12시간(時間) 하였다. 광(光) 처리(處理)에 의(依)한 chlorophyll의 감소(減少) 현상(現狀)은 blue 광하(光下)에서 뚜렷하였고, red 광구(光區)는 white 광구(光區)와 차이(差異)가 없었으며, chlorophyll a와 b도 blue 광(光)에 의한 total chlorophyll의 감소(減少) 현상(現狀)과 동일(同一)한 경향을 나타내었다. Total carotenoid는 blue 광(光) 처리구(處理區)에서 생성(生成)이 촉진되었고 (약(約) 31%), 착색도(着色度)(color score)와 chlorophyll의 영향(影響)과는 달리 대조구(對照區)인 white 광구(光區)에 비(比)하여 red 광(光) 처리구(處理區)에서 함량(含量)이 적었다. 그러나 ${\beta}-carotene$은 red 광(光)의 효과는 없었고, blue 광구(光區)에서 0.71 mg%-f.w.로 white 구(區)의 0.56 mg%-f.w.에 비(比)하여 많았다. 따라서 추숙(追熟) 시(時) 착색(着色) 효과는 blue 광하(光下)에서 현저하게 나타났다. 신미(辛味) 성분(成分)(capsaicin) 역시 red 광(光)의 효과를 인정(認定)할 수 없었고 다만 blue 광하(光下)에서 다소 많았다.

• 한국약용작물학회지
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• 제17권1호
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• pp.21-25
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• 2009

The results on the useful functional compounds of kenaf (Hibiscus cannabinus L.) leaves cultivated in reclaimed lands and the biological activity effects of extracts were as follows. On 98 days after sowing (DAS) Tainung-2 showed the highest total chlorophyll content (1.68 mg/g), and on 141 DAS Dowling showed higher chlorophyll content (1.50 mg/g) than the other two did. In all cultivars the total chlorophyll content was higher on 141 DAS than on 98 DAS. Total polyphenol and total flavonoid contents were the highest in Tainung-2 (30.50 mg/g and 57.03 mg/g, respectively), and total polyphenol and total flavonoid contents (30.50 mg/g and 57.03 mg/g, respectively) were the highest in 30% ethanol extraction. Ascorbic acid contents were higher on 141 DAS than on 98 DAS in three cultivars. SOD activities of kenaf leaf extract were generally over 90%. DPPH radical scavenging activity of Tainung-2 was higher than others.