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Effects of Physico-chemical and Microbiological Inhibitors for Odour gas Evolution in the Fermentation of Liverstock Feces (가축(家畜) 분뇨(糞尿) 발효시(醱酵時) 악취(惡臭)가스 생성억제제(生成抑制劑) 시용(施用) 효과에 관(關)한 연구(硏究))

  • Yun, Sei-Young;Lee, Sang-Kyu
    • Korean Journal of Soil Science and Fertilizer
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    • v.25 no.1
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    • pp.62-69
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    • 1992
  • A series of laboratory experiment was conducted to find out the effects of physico-chemical, microbiological and commercial inhibitors on the odorous gas evolution in the fermentation of livestock feces. The results obtained were summarized as follows. 1. The rate and amount of evolution of gas were the highest at 7 days after incubation, thereafter gradually decreased until 24 days after incubation. 2. The rate and amount of gases were evolved in order of $CO_2>N_2O>CH_4>NH_3>N_2S$, respectively. 3. The highest amount of methane gas was evolved from the poultry feces, those of carbon dioxide and nitrous oxide were evolved from the pig feces, and that of hydrogen sulfide was dominantly evolved from the cattle feces. 4. Negative correlation were obtained between the total amounts of $NH_3$ and $CH_4$, $CO_2$ and $CH_4$, $N_2O$ and $CH_4$, $N_2O$ and $CH_4$, while positive correlations were obtained between the amounts of $CO_2$ and $N_2O$, $CO_2$ and $NH_3$, and $NH_3$ and $N_2O$, respectively. 5. There was no significantly inhibiting effect obtained that the application of commercial gas inhibitor as VK 88. On the other hand there was significantly inhibiting effect obtained when application of fertile paddy soil and photosynthetic bacteria to the fermentation of livestock feces.

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A Study on the Classified Jang(Fermented Soybean) in Goryeo and Chosun Dynasty Period (고려시대 및 조선시대 장류)

  • Ann, Yong-Geun;Woo, Nariyah
    • The Korean Journal of Food And Nutrition
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    • v.25 no.3
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    • pp.460-482
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    • 2012
  • On the basis of the cookbooks and Data Base of the Korean Classics(http://db.itkc.or.kr/itkcdb/mainIndexIframe.jsp), this paper analyzed the fermented soybean listed in the general documents of the Chosun Dynasty(1392~1897) and the Goryeo Dynasty(918~1392). In the Goryeo Dynasty, there are 15 kinds of Jang(soybean paste or solution), among which are Jang (soybean paste fermented by mold)(6 documents), Yeomgjang, Yeomshi(2), and Gaejang(1). However, the cookbook at that time is defunct. The Goryeo Court relieved the famine-stricken people by proving them with Jang. In the Chosun Dynasty, 111 kinds of Jang were listed in the general documents, and 153 kinds in cookbooks. There were 55 kinds of general Jang, such as Jang(204), Yeomjang(63), Chojang, Goojang(7), and Gaejang(6), are listed in the general documents, and in the cookbooks, there are 55 kinds of Jang, such as Sookwhangjang(9 cookbooks), Daemaekjang(8), Myeonjang(8), Saengwhangjang (8), and Yooinjang(8), and among them, 13 kinds belong to the Chinese origin. A total of 9 Kinds of Ganjang(soybean solution fermented by mold), such as Soojang(30), Cheongjang(23), Gamjang(8), and Ganjang(3) are found in the general documents. In the cookbooks, 12 kinds of Jang, as Cheongjang(10), Cheonrijang(4), Ganjang(3), and etc., are listed. There were 9 kinds of Gochoojang(red pepper-soybean paste), such as Chojang(12), Gochojang(3), and etc., are listed in the general documents, and 9 kinds as Gochojang(7), Manchojang(7), rapid Manchojang(4), and etc., are in the cookbooks. In addition, 16 Kinds of Yookjang(fermented soybean-meat paste) as Haejang(15), Hyejang(11), Yookjang(11), and etc., are found in the documents, and 22 kinds as Nanjang(9), Gejang(6), Yookjang(5), Shoigogijang(4), and etc., are in the cookbooks. Eighteen Kinds of Shi(soybean paste fermented by bacteria) as Yeomshi(40), Shi(35), Shijang(6), and etc., are recorded in the documents, and 19 kinds as Jeonkookjang(6), Shi(4), Sooshijang(4), and etc., are in the cookbooks, and among them 11 kinds belong to the Chinese origin. Six kinds of Jipjang(aqueous soybean paste) as Jipjang(7), Uoopjang(4), Pojang (2), Jangzoop(2) are recorded in the documents, and 15 kinds as Jipjang(9), Zoopjeo(7), and Hajeoljipjang(5) are in the cookbooks. Soybean paste, or solution for relieving hunger is not recorded in the documents. However, the Chosun court, for the purpose of relieving famine-stricken people, used general Jang. Such 21 Jang to relieve the famine-stricken people as Pojang(7), rapid Jang(6), and Sasamgilgyeongjang(4) are listed in the cookbook. Geonjang(dried soybean paste), Nanjang (egg-soybean paste), Doojang(soybean paste), Maljang(random soybean paste), Myeonjang(wheate-soybean paste), Sodoojang (red bean-soybean paste), Yookjang(soybean-meat paste) and Jang(soybean paste) are recorded in the documents, as well as in the cookbooks. Chinese-original Jang and Shi are recorded in the cookbooks, with no list in the general documents. Therefore, it seems that it didn't pass down to the general public.

Antimicrobial Resistance of Bacteria Isolated from Bronchoalveolar lavage (BAL) in Patients with Lung Infiltrations in Burn and Non-Burn Intensive Care Unit (화상 중환자실과 비화상 중환자실 폐 침윤 환자에서 기관지폐포 세척술(BAL)을 통해 획득한 호흡기 검체에서 분리된 균주에 대한 항균제 내성 실태)

  • Kim, Jong-Yeop;Kim, Cheol-Hong;Park, Soo-Hee;Ko, Yoo-Sang;Kim, Mi-Jung;Kang, Hye-Ryun;Hwang, Yong Il;Park, Yong-Bum;Jang, Seung-Hoon;Woo, Heungjeong;Kim, Dong-Kyu;Lee, Myung-Koo;Hyun, In-Gyu;Jung, Ki-Suck
    • Tuberculosis and Respiratory Diseases
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    • v.62 no.6
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    • pp.506-515
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    • 2007
  • Background: Nosocomial pneumonia in an intensive care unit (ICU) is associated with a high mortality rate. Diagnosing a respiratory tract infection in critically ill patients is still difficult but detailed information for the pathogens is needed to establish an adequate antimicrobial treatment. This study examined the causative organisms and their antimicrobial resistance using bronchoalveolar lavage (BAL) from patients suspected of having pneumonia in the ICU. Methods: From January 2004 to June 2006, ICU patients with diffuse lung infiltration were prospectively enrolled. The BAL was used to diagnose the respiratory infection, with 104 > or = organisms considered a positive result. The most common organisms and their antimicrobial resistances were analyzed from the quantitative BAL cultures in the burn ICU and non-burn ICU. Results: A total 72 patients were included, 35 (M 29, F 6) in the burn ICU and 37 (M 26, F 11) in the non-burn ICU. 27 patients (77.1%) in the burn ICU and 22 patients (59.5%) in the non-burn ICU met the criteria for a positive BAL culture. The major pathogens were Staphylococcus aureus, Acinetobacter species and Pseudomonas aeruginosa. All strains (100%) of Staphylococcus aureus isolated from BAL (9 cases) were methicillin-resistant (MRSA) in the burn ICU, but 5 strains (71.4%, 7 cases) were MRSA in the non-burn ICU. Regarding Pseudomonas aeruginosa, the rate of resistance to amikacin, ciprofloxacin, cefepime, imipenem, ceftazidime, piperacillin/tazobactam in the burn and non-burn ICU ranged from 45.5% to 90% and 25% to 50%, respectively. In addition, the rate of resistance of Acinetobacter species to the above drugs in the burn and non-burn ICU ranged from 81.8% to 100% and 62.5% to 100%, respectively. Conclusions: These results are expected to provide useful guidelines for choosing the effective empirical antimicrobial therapy in patients with lung infiltrations in the burn and non-burn ICU.

Effects of Momordica charantia Saponins on In vitro Ruminal Fermentation and Microbial Population

  • Kang, Jinhe;Zeng, Bo;Tang, Shaoxun;Wang, Min;Han, Xuefeng;Zhou, Chuanshe;Yan, Qiongxian;He, Zhixiong;Liu, Jinfu;Tan, Zhiliang
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.4
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    • pp.500-508
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    • 2016
  • This study was conducted to investigate the effects of Momordica charantia saponin (MCS) on ruminal fermentation of maize stover and abundance of selected microbial populations in vitro. Five levels of MCS supplements (0, 0.01, 0.06, 0.30, 0.60 mg/mL) were tested. The pH, $NH_3-N$, and volatile fatty acid were measured at 6, 24, 48 h of in vitro mixed incubation fluids, whilst the selected microbial populations were determined at 6 and 24 h. The high dose of MCS increased the initial fractional rate of degradation at t-value = 0 ($FRD_0$) and the fractional rate of gas production (k), but decreased the theoretical maximum of gas production ($V_F$) and the half-life ($t_{0.5}$) compared with the control. The $NH_3-N$ concentration reached the lowest concentration with 0.01 mg MCS/mL at 6 h. The MSC inclusion increased (p<0.001) the molar proportion of butyrate, isovalerate at 24 h and 48 h, and the molar proportion of acetate at 24 h, but then decreased (p<0.05) them at 48 h. The molar proportion of valerate was increased (p<0.05) at 24 h. The acetate to propionate ratio (A/P; linear, p<0.01) was increased at 24 h, but reached the least value at the level of 0.30 mg/mL MCS. The MCS inclusion decreased (p<0.05) the molar proportion of propionate at 24 h and then increased it at 48 h. The concentration of total volatile fatty acid was decreased (p<0.001) at 24 h, but reached the greatest concentration at the level of 0.01 mg/mL and the least concentration at the level of 0.60 mg/mL. The relative abundance of Ruminococcus albus was increased at 6 h and 24 h, and the relative abundance of Fibrobacter succinogenes was the lowest (p<0.05) at 0.60 mg/mL at 6 h and 24 h. The relative abundance of Butyrivibrio fibrisolvens and fungus reached the greatest value (p<0.05) at low doses of MCS inclusion and the least value (p<0.05) at 0.60 mg/mL at 24 h. The present results demonstrates that a high level of MCS quickly inhibits in vitro fermentation of maize stover, while MCS at low doses has the ability to modulate the ruminal fermentation pattern by regulating the number of functional rumen microbes including cellulolytic bacteria and fungi populations, and may have potential as a feed additive applied in the diets of ruminants.

Effects of extreme heat stress and continuous lighting on growth performance and blood lipid in broiler chickens (연속조명과 폭염 스트레스가 육계의 혈액지질 및 성장능력에 미치는 영향)

  • Park, Sang-Oh;Hwangbo, Jong;Ryu, Chae-Min;Yoon, Jae-Sung;Park, Byung-Sung;Kang, Hwan-Ku;Seo, Ok-Suk;Chae, Hyun-Seok;Choi, Hee-Chul;Choi, Yang-Ho
    • Journal of the Korean Applied Science and Technology
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    • v.30 no.1
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    • pp.78-87
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    • 2013
  • In this study, the effect of extreme heat diet on growth performance, lymphoid organ, blood immunoglobulin and cecum microflora change in broilers exposed to continuous lighting and extreme heat stress (EHS) was studied. Broilers raised under normal environment temperature ($25^{\circ}C$ or extreme heat stress temperature ($33{\pm}2^{\circ}C$, and consumed chow diet (CD) or extreme heat stress diet (EHSD). Five hundred Ross 308 day-old commercial broilers were arranged in a completely randomized block design of 5 treatment groups with 4 repetitions (25 heads per repetition pen). The broilers were divided into: T1 (normal environment+CD), T2 (EHS+CD), T3 (EHS+EHSD in which the tallow in CD was substituted by soy oil and contained 5% molasses), T4 (EHS+EHSD in which the tallow in CD was substituted by soy oil and contained 5% molasses, and 1.5 times more methionine and lysine than CD), and T5 (EHS+EHSD in which the tallow in CD was substituted by soy oil, contained 5% molasses, 1.5 times more methionine and lysine than CD, and 300ppm of vitamin C). The EHS significantly reduced the body weight gain and feed intake. The blood immunoglobulin, bursa of Fabricius, thymus, and spleen weight were significantly reduced when broilers were exposed to EHS. Compared to the normal environment temperature group, the cecum Lactobacillus sp. was low in the EHS treatment group, while Escherichia sp., Salmonella sp. and total aerobic bacteria in the EHS treatment group were high. A statistically significant difference was acknowledged between the treatment groups.

Effects of Amendments on Ginseng Root Rot Caused by Fusarium solani Population Changes of the Microorganisms in Soil (토양(土壤)개량(改良)이 Fusarium solani 에 의한 인삼근부병과(人蔘根腐病)과 미생물(微生物) 변동(變動)에 미치는 효과(效果))

  • Son, Suh-Gyu;Shin, Hyun-Sung;Lee, Min-Woong
    • The Korean Journal of Mycology
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    • v.13 no.1
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    • pp.41-47
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    • 1985
  • Eighteen plant residues were added to soil and the amended soil was inoculated with chlamydospores (5,000 cells/g soil) of Fusarium solani causing root rot of ginseng in soil to test the effects of amendment using pea (Pisium sativa L.) as an index plant. Distributional conditions of microogranisms in soil were compared with each other before and after dealing with soil amendment by plant debries. Infection rate by index plant's infectivity showed a higher degree in the treatment of wheat crushed than in control group, and followed by stalk of sweet potato, chinese cabbage, ginseng leaves and soybean pod. On the other hand, the de­creasing order of infection rate was root of garlic, welsh onion, cabbage leaf and stalk, green onion stalk, wheat straw and barley straw. In comparison with control group, the propagules of fungi increased in the treatment of ginseng leaves, soybean ground, wheat crushed, maize stalk, and chinese cabbage, but decreased in the root of garlic, cabbage, and barley straw. Population of total bacteria increased in the treatment of soybean ground, chinese cabbage, radish stalk, welsh onion, and wheat crushed, but decreased in barley straw, tobaco root, ginseng stalk, and wheat straw. The numbers of actinomycetes increased only in the treatment of soybean ground in a comparison with control and also decreased in the garlic stalk and tobaco root. The propagules of Fusarium spp increased in the treatment of chinese cabbage, welsh onion, radish stalk, wheat crushed, and sweet potato stalk, wheat crushed, and sweet potato stalk, but decreased in the treat­ment of wheat straw, ginseng leaves, and cabbage than control.

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A Study on Quality Characteristics of Sourdough Breads with Addition of Red Yeast Rice (홍국(Red Yeast Rice)을 이용한 Sourdough Bread의 품질특성 연구)

  • Lee, Jae-Hoon;Kwak, Eun-Jung;Kim, Ji-Sang;Lee, Kwang-Suck;Lee, Young-Soon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.6
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    • pp.785-793
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    • 2007
  • This study was carried out to investigate the effects of red yeast rice addition on the physical characteristics of sourdough starters ($SD1{\sim}4$) and the quality characteristics of sourdough bread (CSB, $SDB1{\sim}3$). Moisture contents of red yeast rice, wheat flour, and rye flour were 6.15, 12.53, and 8.56%; ash contents were 0.15, 0.44, and 1.64%; protein contents were 7.30, 12.57, and 11.18%; crude lipid contents were 0.97, 1.16, and 2.49%, respectively. The pH decreased with increasing red yeast rice addition. Reducing sugar and total sugar increased with increasing red yeast rice addition. Lactic acid bacteria were not detected from 0 day for all samples and was maximum on the 2nd day. The yeast counts of SD1 were not detected from $0{\sim}4th$ day, but $SD2{\sim}4$ increased until the 3rd day. Peak time, peak value, and width of tail of CSB were higher than $SDB1{\sim}3$, and they increased with increasing red yeast rice contents of sourdough. The fermentation expansive power of $SDB1{\sim}3$ was higher than that of CSB. Baking loss and specific volume of SDB1 were higher than other samples and when baking loss of CSB and SDB1${\sim}$3 increased, the specific volume increased. L values decreased with increasing red yeast rice contents of sourdough bread whereas, a and b values increased. Springiness and brittleness of CSB and gumminess of SCB1 were lower than other samples. Springiness, brittleness, and gumminess increased with increasing red yeast rice content of sourdough bread. The sensory evaluation indicated that color, hardness, springiness, sweetness, and sourness increased with increasing red yeast rice content of sourdough bread. Aircell non-uniformity of SDB1 was lower than other samples, whereas off-flavor was higher than other samples.

Analysis of Microbial Contamination and Preservatives in Children's Favorite Foods Around Elementary Schools in Gyeonggi and Incheon (경인지역 초등학교 주변 어린이 기호식품의 미생물 오염도 및 보존료 검사)

  • Park, Shin-Young;Choi, Jin-Won;Yeon, Ji-Hye;Lee, Min-Jeong;Ha, Sang-Do;Park, Ki-Hwan;Moon, Eun-Sook;Ko, Myung-Hee;Lee, Ji-Hyun;Cho, Yu-Sean;Ryu, Kyung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.2
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    • pp.224-230
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    • 2006
  • Microbial contamination levels and legal preservative appropriation in child foods sampled from the neighborhood of elementary schools were investigated. Contamination levels of total aerobic bacteria in seasoned dried fish slices, bread and snacks, sausages, sugar products and dumplings were $1.70\~6.91,\;1.40\~6.66$, 4.50, $3.48\~5.88$, and $4.79\~4.82\;log_{10}$ CFU/g, respectively. Coliforms in four kinds of foods except for dumplings were $2.30\~6.60,\;4.22\~~5.98$, 2.00, and $2.78\;log_{10}$ CFU/g, respectively. Yeasts and molds in those foods were $0.10\~4.23,\;1.66\~4.91,\;1.46\~1.91,\;1.56\~4.26$, and $1.12\~1.84\;log_{10}$ CFU/g, respectively. S. aureus was isolated in $18\%$ of seasoned dried fish slices ($1.00\~2.84\;log_{10}$ CFU/g), $33\%$ of bread and snacks ($1.70\~1.79\;log_{10}$ CFU/g), $50\%$ of sausages ($3.28\;log_{10}$ CFU/g), $22\%$ of sugar products ($2.16\~2.88\;log_{10}$ CFU/g), and $100\%$ of dumplings $(1.18\~3.31\;log_{10}\;CFU/g)$ B. cereus was isolated in $21\%$ of seasoned dried fish slices $(0.70\~2.48\;log_{10}\;CFU/g)$, $50\%$ of bread and snacks $(0.70\;log_{10}\;CFU/g)$, and $11\%$ of sugar products $(0.30\;log_{10}\;CFU/g)$. Both E. coli and Salmonella spp. were not isolated in all samples. Preservative was only labeled on four products among 15 products but preservative on 13 products including 4 products haying an indication of preservative were not detected. Moreover, $0.30\%$ of sorbic acid was detected in one of Squid products . The results of this study indicated that the hygienic level of child foods in Gyeonggi and Incheon was very poor and need to be improved.

Clinical characteristics of deep neck abscess in children; 15 year experience in a university hospital (소아 심경부농양의 임상적 고찰 : 일개 대학병원에서 15년간의 경험)

  • Goo, Min-Ji;Yeom, Jeong-Suk;Kim, Eo-Jin;Park, Jung-Je;Seo, Ji-Hyun;Park, Eun-Sil;Lim, Jae-Young;Park, Chan-Hoo;Woo, Hyang-Ok;Youn, Hee-Shang
    • Clinical and Experimental Pediatrics
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    • v.50 no.7
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    • pp.649-654
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    • 2007
  • Purpose : The objective of this study was to clarify the presentation, associated preceding illness, pathologic organisms, treatment and outcome of deep neck abscess in children according to age and location. Methods : We retrospectively reviewed the in-patient charts of children treated at our hospital for deep neck abscess. Thirty-five such patients were identified as having been treated from March 1990 to December 2005. Results : A total of 35 were enrolled in our study: 25 boys and 10 girls. Their ages ranged from 11 months to 15 years. Presenting symptoms included mass, fever, irritability, trismus and dysphagia. The most commonly known associated preceding illness was viral upper respiratory infection (53%). The most common site of infection was the submandibular space (37%). Bacteria was identified in 16 patients. The most common pathogen was Staphylococcus aureus. Thirteen (37%) children recovered from the infection with conservative treatment and twenty-four (68%) children received surgical drainage. The duration of hospitalization was longer in the group who underwent surgery than in the group who were managed with conservative treatment. No complication occurred. Conclusion : Unexplained torticollis, trismus or irritability in children were suggestive of deep neck abscess. Our results demonstrate that deep neck abscesses in children is respond well to conservative treatment if diagnosed early.

Herbicidal and Antifungal Activities of the aqueous extracts of Several Naturalized Plants (수종의 귀화식물 수용성추출물의 제초 및 항균 활성 탐색)

  • Hyoun, Do-Gyoung;Song, Jin-Young;Kim, Tae-Keun;Jung, Dae-Cheon;Song, Sang-Churl;Kang, Young-Sik;Cha, Jin-Woo;Lee, Hee-Sean;Yang, Young-Hoan;Kim, Hyoun-Chol;Song, Chang-Khil
    • Korean Journal of Organic Agriculture
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    • v.22 no.2
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    • pp.303-319
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    • 2014
  • The study researched germination of the plants and growth of experimented bacteria according to concentration of water extract in order to provide basic data for developing natural agricultural resources by using naturalized plants including Solidago altissima, Amaranthus retroflexus and Sida spinosa. As concentration of water extract increased, most of test plants showed a decrease in relative germinability. Sida spinosa(r=-0.540, p<0.01), Physalis wrightii(r=-0.693, p<0.01), Amaranthus retroflexu(r=-0.724, p<0.01), Solidago altissima(r=-0.728, p<0.01) and Eclipta prostrata(r=-0.779, p<0.01) showed tendency of decrease in relative germinative power in order, respectively. For average germination period, as concentration of the processed group increased, the time for germination increased (r = 0.769, p<0.01) and according to donor plants and test plants, there was a little difference. Also, as concentration of water extract of donor plant, length of above-aerial part(r=-0.587, p<0.01), length of underground part(r=-0.741, p<0.01), fresh weight(r=-0.574, p<0.01) and generation of root hair decreased. An then, for growth of test fungi according to concentration of water extract of donor plants, growths of Botrytis cinerea(r=-0.266, p<0.05), Diaporthe citri(r=-0.323 p<0.01), Colletotrichum gloeosporioides(r=-0.512, p<0.01), Pythiumultimum(r=-0.581, p<0.01) and Rhizoctonia solani(r=-0.806, p<0.01) were repressed in order, respectively. For total amount of content of phenol with herbicidal and Antifungal activities, S. altissima $17.3{\pm}0.5mg/g$, A. retroflexus $13.1{\pm}0.3mg/g$, P. wrightii $12.0{\pm}0.4mg/g$, S. spinosa $9.5{\pm}0.1mg/g$ and E. prostrata L. $4.1{\pm}0.1mg/g$ showed in order, respectively. As these results are summarized, donor plants which were naturalized, have competitive advantage because they release phenolic compounds with allelopathic effect and affect on germination, growth and fungi growth on underground flora compared to native plants and they have eligibility for natural herbicide and germicide.