• 한국식품영양과학회지
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• 제43권10호
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• pp.1619-1626
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• 2014

마리네이드, 간장소스, 된장소스 및 고추장소스를 대상으로 총균수 및 Bacillus cereus 접종포자에 대해 초고압, 줄가열, 항균물질에 단독처리나 hurdle 복합처리에 의한 저감효과를 분석하였다. 줄가열 처리는 된장 및 고추장소스의 총균과 접종포자에 대해서 1.0~2.0 log 범위의 저감효과를 나타내었다. 한편 J($85^{\circ}C$), A(EtOH 3.0%) 및 P(550 Mpa, 5분)를 허들로 설정하였을 경우 된장소스의 총균수는 JA 처리구가 0.35 log, JP 처리구가 0.92 log 및 JAP 처리구가 1.21 log 감소하였고, 고추장소스의 총균수는 JA 처리구가 1.26 log, JP 처리구가 1.7 log 및 JAP 처리구가 1.47 log 감소하였다. 포자의 경우 된장소스는 AP 처리구가 0.50 log, JP와 JAP 처리구는 각각 1.42 log 및 1.38 log로 감소 효과가 증가하였고, 고추장소스는 AP 처리구가 0.47 log, JA 처리구가 3.3 log, JP와 JAP 처리구가 모두 3.45 log만큼 크게 감소하였다. 상기 소스의 총균과 포자에 대한 처리구는 $30^{\circ}C$에서 8주간 저장 시 대조구 대비 유사하거나 낮은 수준을 유지하였다. 따라서 소스류 중에서 고추장소스와 된장소스는 줄가열과 초고압을 연계한 허들처리를 통해 총균수와 B. cereus 포자를 제어할 수 있을 것으로 기대된다.

• 미생물학회지
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• 제3권1호
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• pp.7-10
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• 1965

Higher number in colony counts in coliforms, total aerobes, and anerobes was obtained from marketable soy-bean mash than front that in fermented tank. The ratio between coliform contamination and total viable cells was higher in red pepper mash than in soy-bean mash. E. coli, contaminated in soy-bean mash persisted longer at low temperature ($0^{\circ}C$-$^5{\circ}C$) than at room temperature and they vanished after seven days of storage at room temperature. At 30.deg.C and 35.deg.C, these organisms were more effected than at room temperature. E. coli cells, inoculated in red pepper mash, were not recovered at room temperature after five days incubation. Soy-bean mash, completely fermented at normal conditions, were detected to contain $10^8$- $10^9$ organisms per gram of sample. On the contrary, marketable soy-bean mash were found to have more than 10$^{9}$ per gram samples. Since samples were found to have more than $10^9$ aerobes and anaerobes per gram, contamination of coliforms seemed to be apparent.

• 한국식품과학회지
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• 제33권5호
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• pp.545-550
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• 2001

Effects of high hydrostatic pressure on pH, titratable acidity, color, hardness and microorganisms of Chinese Cabbage Kimchi were investigated. Kimchi was pressurized at $200{\sim}600$ MPa for 5 min. There were no significant differences in color and hardness between control and pressurized Kimchi (p>0.05). Total aerobes and lactic acid bacteria were effectively inactivated by high hydrostatic pressure above 400 MPa. Changes in pH, titratable acidity, color, hardness and microbial counts for 4 weeks storage of Kimchi were investigated Kimchi was pressurized at 400 MPa for 5 min and stored at $4^{\circ}C$. The pH of control decreased to 3.94 but pressurized Kimchi maintained its initial pH value throughout the storage. The color of control showed significantly low values compared with pressureized Kimchi (p<0.05), but hardness was not significantly changed (p>0.05). Total aerobes and lactic acid bacteria in the control were reduced from the initial value of $10^8{\sim}10^9$ CFU/mL to $10^6$CFU/mL after 4 weeks storage. Whereas microbial counts in pressurized Kimchi was maintained about $10^3{\sim}10^4$ CFU/mL during storage.

• Journal of Nutrition and Health
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• 제32권1호
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• pp.64-64
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• 1999

The relations between micorfloral compositions and environmental factors of 32 Korean infants were sought through statistical analysis after examination of fecal bacterial and questions to their mothers about feeding experiences at three different times for each subjects, about one week after birth, before weaning (2-3 months after birth), and after weaning 95-7 months after birth). The majority of mothers fed their infants cereal foods after the age of 4 months and began weaning with fruit juice and commercial weaning foods. Defection frequencies and fecal pH of infants decreased significantly during the examination period and 37.5% of total infants in fecal samples increased significantly during the examination period, which means that the kinds of bacterial genera increase with aging of infants. Frequencies of streptococci were significantly higher in infants fed delivered by Caesarian section than infants delivered naturally. Frequencies of clostridia were significantly higher in infants fed with cereal food before 4 months of age than after 5 months. The infants fed with probiotics showed significantly higher frequencies of veillonella at about 1 week old. They also showed significantly higher frequencies of clostridia before weaning than the infant fed with no probiotics, but significantly lower frequencies of C. perfringens before weaning. The infants fed with probiotics showed significantly higher number of streptococci at the age of about 1 week and significantly higher numbers of total aerobes before weaning, but significantly lower numbers of bacteroides after weaning than their counter parts. The fecal pH was directly proportional to the number of clostridia, klebsiella, and total aerobes at about 1 week after birth, to the number of E. coli before weaning, and to the number of streptococci and clostridia after weaning. Fecal pH had a negative relationship to the total number of anaerobes in 1-week-old infants. The infants that had diarrhea during lactation showed higher frequencies of bacteroides before weaning than those that didn't.

• Journal of Nutrition and Health
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• 제32권1호
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• pp.67-74
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• 1999

The relations between micorfloral compositions and environmental factors of 32 Korean infants were sought through statistical analysis after examinaton of fecal bacterial and questions to their mothers about feeding experiences at three different times for each subjects, about one week after birth, before weaning (2-3 months after birth), and after weaning 95-7 months after birth). The majority of mothers fed their infants cereal foods after the age of 4 months and began weaning with fruit juice and commercial weaning foods. Defection frequencies and fecal pH of infants decreased significantly during the examination period and 37.5% of total infants in fecal samples increased significantly during the examination period, which means that the kinds of bacterial genera increase with aging of infants. Frequencies of streptococci were significantly higher in infants fed delivered by Caesarian section than infants delived naturally. Frequencies of clostrida were significantly higher in infants fed with cerealfood before 4 months of age than after 5 months. The infants fed with probiotics showed significantly higher frequencies of veillonella at about 1 week old. They also showed significantly higher frequencies of clostridia before weaning than the infant fed with no probiotics, but significantly lower frequencise of C. perfringens before weaning. The infants fed with probiotics showed significantly higher number of streptococci at the age of about 1 week and significantly higher numbers of total aerobes before weaning, but significantly lower numbers of bacteroides after weaning than their counter parts. The fecla pH was directly proportional to the number of clostridia, klebsiella, and total aerobes at about 1 week after birth, to the number of E. coli before weaning, and to the number of streptococci and clostridia after weaning. Fecal pH had a negative relationship to the total number of anaerobes in 1-week-old infants. The infants that had diarrhea during lactation showed higher frequencies of bacteroides before weaning than those that didn't.

• 한국식품과학회지
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• 제31권1호
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• pp.54-61
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• 1999

본 연구는 해동 중 발생하는 drip 손실, 미생물의 오염, 표면의 과열 등을 방지하면서 급속 해동 방법을 찾기 위해 동결 돈육에 여러 가지 해동 방법을 적용하였다. 4, 28, $50^{\circ}C$에서의 해동과 2,450, 915 MHz의 microwave를 이용하여 해동을 실시하였고, 915 MHz microwave 해동은 출력을 5, 10, 15 kW로 변화시키면서 단일 조사와 $120^{\circ}C$의 열풍을 함께 조사한 경우를 해동시간, 온도분포, drip 손실, 보수력, 표면색도, 총세균수를 측정하였다. 915 MHz microwave를 이용한 해동은 열전도에 의한 해동보다 2백배 이상 시간이 단축되었고, 2,450 MHz microwave를 이용한 해동 방법의 침투깊이의 제한에서 발생되는 문제를 개선시킬 수 있었으며 drip 손실, 보수력, 총세균수에 있어서 효과가 컸다. 저주파수(915 MHz), 저출력(5 kW)의 microwave와 대류 가열을 병용하여 품질 저하를 최소한으로 방지하면서 급속해동을 위한 산업적 이용이 가능한 것으로 기대할 수 있었다.

• Journal of Nutrition and Health
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• 제31권3호
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• pp.305-314
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• 1998

Microflora in 14 breast-fed(BF) and 15 formula-fed(FF) infants were investigated before (2-3 months after birth) and after weaning (5-7 months birth) to find the floral differences between the two lactation groups and the floral changes after weaning. Bifidolbacteria showed the highest count among the species of microflora in the BF group before and after weaning, but in the FF group, streptococci showed the highest count before weaning and bifidobacteria after weaning. Before weaning , the count of bifidobacteria, lactobacilli and total acaerobes were significantly higher in the BF group than in the FF. However, the frequencies of clostridia and klesidella , along with the counts of streptococci and eubacteria, were higher in the FF group. After weaning , the only difference was that the total count of aerobic bacteria was higher in the BF group. The counts of streptococci in the BF group and lactobacilli and total anaerobes in the FF group after weaning were significantly higher than before weaning. However, the count of total aerobes in the FF group after weaning was significantly lower than the count before weaning.

• 한국식품과학회지
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• 제27권6호
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• pp.991-996
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• 1995

비열처리공법 중 하나로 새롭게 주목받고 있는 초고압처리를 신선초 녹즙에 적용하여 미생물의 살균정도 및 갈변효소의 불활성화정도를 살펴보았다. 실험에는 $3800\;kg_f/cm^2{\sim}6700\;kg_f/cm^2$ 범위의 압력에서 $3{\sim}17\;min$동안 처리하였으며 반응표면분석법(RSM)을 이용하여 미생물 및 효소 불활성화의 최적조건을 구하고자 하였다. 반응표면분석결과 $5700\;kg_f/cm^2$ (558.6 MPa)에서 7.16 min 처리할 때 3.44 log cycles의 총균수 감소효과를 얻을 수 있을 것으로 예상되었다. 대장균의 경우, 모든 처리 조건에서 완전살균이 가능하였다. Peroxidase나 PPO와 같은 갈색화효소는 초고압처리에 의해서 쉽게 불활성화 되지 않았으며, 미생물이나 효소의 불활성화는 처리시간보다는 압력에 더욱 의존함을 알 수 있었다.

• Journal of Microbiology
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• 제33권4호
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• pp.273-277
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• 1995

Although trout farming is well established in Korea, very little information is available on the composition of intestinal microflora in rainbow trout (Salmo gairdnerii). In 1994, from October through November, we investigated the composition and succession of intestinal bacteria. As fish grew, total viable counts increased dramatically until 45 days after fertilization when anaerobes started to appear on the media. After this time, they increased steadily. Ten aerobic generic were identified and Gram negative bacteria constituted 85% of total isolates. Among these, Pseudomonas, Eikenella, and Alcaligenes were the three major genera. Six anaerobic genera were isolated and identified. The ratio of anaerobes to aerobes was about 1 : 1 in adult trout and the composition of genera was changed under different conditions.

• Preventive Nutrition and Food Science
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• 제9권3호
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• pp.218-221
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• 2004

Irradiated red pepper powder (IRPP) was tested for its ability to retard fermentation and to maintain a high quality of Kimchi by the reduction of the initial microbial load. Kimchi containing IRPP at the doses of 0, 5, 10, 15, or 20 kGy was prepared. Quality indices for Kimchi in this study were pH, titratable acidity, reducing sugar content, total microbial count, lactic acid bacterial load, and sensory evaluation. Based on the pH and titratable acidity, the Kimchi with IRPP showed a retarded fermentation until 15 days. The number of the total aerobes and lactic acid bacteria of the Kimchi with IRPP were lower by about 1 log CFU/mL compared to control at day 0, however, the counts increased to 8.5 log CFU/mL after 10 days, which was similar to the control group. Kimchi that was fermented with 5 kGy IRPP was better than control and other treatments in odor and color, whereas the control scored highest in taste. Addition of IRPP showed a limited retardation of Kimchi fermentation without other quality deterioration.