• 한국약용작물학회지
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• 제18권1호
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• pp.15-21
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• 2010

This study was conducted to investigate the effects of Lycii fructus and Astragalus membranaceus mixed extracts on immunomodulators and prevention in a streptozotocin-induced diabetes rat model. A total of 28 male rats were divided into four dietary groups and fed a commercial diet (A), commercial diet plus induced diabetes by a streptozotocin (STZ) injection (B), induced diabetes by STZ plus medicinal crop extracts(I&$H^{(R)}$) diet (C), and medicinal crop extracts (I&$H^{(R)}$) diet (D). Immunoblotting analyses revealed cytokine expression, and ELISA analyses revealed immunoglobulin E and nitric oxide production. As a results, the tumor necrosis factor-$\alpha$ (TNF-$\alpha$) and inducible nitric oxide synthase (iNOS) as a inflammatory cytokine were decreased. Interleukin-6 (IL-6) and signal transducer and activation of transcription 3 (STAT3) cytokine related in diabetes expression through JAK/STAT3 pathway were also decreased. Furthermore, immunoglobulin E and nitric oxide production were decreased in the serum and lens, respectively. These results suggest that Lycii fructus and Astragalus membranaceus mixed extracts provide positive effects on immunomodulators and prevention in diabetes and eye disease complications.

• Korean Journal of Acupuncture
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• 제22권2호
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• pp.107-125
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• 2005

Objectives : The aim of this study was to investigate the asthma-suppressive and immuno-regulatory effect of NR-HA(Notopterygii Rhizoma Herbal-acupuncture) at Pyesu(BL13) on OVA(ovalbumin)-induced asthma in mice. Methods : C57BL/6 mice out of all the experimental sloops, except the Normal group and the NR-HA group, were sensitized and challenged with OVA. The mice in the NR-HA group and the OVA-NR-HA group were treated with NR-HA(1%) at Pyesu(BL13). The mice in the OVA-Saline group were injected with saline at Pyesu(BL13). The mice in the OVA-Needle-prick group were treated with a single prick with an injection needle at Pyesu(BL13). NR-HA, saline injection and needle prick were administered for 8 weeks, three times a week Results : in vitro 1. The populations of granulocytes, $CD3e^-/CCR3^+$cells, $CD69^+/CD3e^+$ cells, $CD4^+\;cells\;and\;CD23^+/B220^+$ cells in the OVA-induced asthmatic mouse lungs decreased significantly by NR-HAS(Notopterygii Rhizoma Herbal-acupuncture solution). 2. The lung weight and total cells in lung of the OVA-NR-HA group decreased significantly compared with those of the OVA-Control group. 3. Total leukocytes and eosinophils in BALF of the OVA-NR-HA group decreas ed significantly compared with those of the OVA-Control group. 4. The collagen accumulation in the lung sections of the OVA-NR-HA group decreased significantly compared with that of the OVA-control group. 5. The concentrations of IL-4, IL-5, IL-13, IgE in BALF and serum of the OVA-NR-HA group decreased significantly compared with those of the OVA- Control group. 6. The numbers of $Gr-1^+/CD11b^+,\;CCR3^+,\;CD3e^+, \;CD19^+,\;CD3e^+/CD69^+$ cells in the OVA-NR-HA group decreased significantly compared with those of the OVA-Control group. 7. The mRNA expressions of $TNF-{\alpha}$, IL-5, IL-4 and IL-13 in lung of the OVA-NR-HA group decreased significantly compared with those of the OVA- Control group. 8. The NR-HA group did not show my considerable difference from the Normal group. The OVA-saline group and the OVA-Needle prick group showed suppressive effects on OVA-induced asthma however they were not statistically significant. Conclusion : These results suggest that NR-HA at Pyesu(BL13) is considered to be effective in treating asthma and to be put to practical use in the future asthma clinic.

• 한방안이비인후피부과학회지
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• 제27권3호
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• pp.72-83
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• 2014

Objective: This study was to evaluate whether herbal mixture (HM-A : Houttuynia cordata Thunberg, Rubus coreanus, Rehmannia glutinosa, Prunus yedoensis, HM-B : Houttuynia cordata Thunberg, Rubus coreanus, Rehmannia glutinosa, Angelica gigas nakai) supresses the development of atopic dermatitis in Balb/c mice sensitized by ovalbumin. Methods: Mice were sensitized by intraperitoneal injection of ovalbumin plus aluminum hydroxide hydrate, followed by epicutaneous sensitization for 6 weeks. After induced atopic dermatitis, HM-A and HM-B were orally administrated for two weeks(once a two days) as a 50 mg/kg concentration. After all mice were sacrificed at the end of the experiment, skin and blood were harvested. Results: Oral administration group was reduced the infiltration of eosinophils, mast cells and total T cells on the skin areas as well as blood analysis. Also, cutaneous expression of IL-4,13,17 decreased. Blood IgE level was decreased. Conclusion: These drugs could be potential candidates for the atopic dermatitis.

• 동의생리병리학회지
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• 제19권1호
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• pp.106-113
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• 2005

Asthma is an inflammatory disease of airways that is induced by Th2 cytokines and inhibited by Th1 cytokines. In this study we wanted to investigate the effect of SCT and BJT on eosinophilla and cytokines of BALF in a mouse model established airway inflammmation. Asthma was induced to male c57/bl6 mice. Allergen-specific antibody responses, cytokine$(IL-4,\;IL-5,\;INF-{\gamma})$, and eosinophil inflammation of the airways were investigated on the BALF and splenocyte. SCT and BJT effectively induced $INF-{\gamma}$ and inhibited IL-4, IL-5 as well as eosinophilic inflammation when SCT and BJT were administered. Total Ig E level in the BALF decreased. SCT was more effectiveness than BJT. It is considered that SCT and BJT have faborable effect on the asthma because the asthma specific series of abnormalities in respiratory system were decreased.

• 대한임상검사과학회지
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• 제39권3호
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• pp.201-209
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• 2007

Among the inhalant and foods which caused allergy, which is increasing nowaday, this research has a purpose of studying allergy-causing materials (allergen) which most frequently cause allergy. In order to conduct this research, we selected 100 hundred patients (M : F = 65 : 35) from in Kwangju Christian Hospital, who were tested through allergy multiple antigen simultaneous test (MAST) immunoblot assay (RIDA$^{(R)}$ X-Screen; r-Biopharm Co., Darmstadt, Germany). According to the test, the total IgE positive rate (above Class 2) from the inhalant is 58.3%, and that from food is 35%, respectively. This research showed that the first most allergen was the tick (Derm. farinae/Derm. pteronyssinus), the second most was housedust, and the third was the hair of pets such as cats and dogs; 65%, 35%, 26.3% in inhalant panel, and 52.5%, 40%, 50% in food panel, respectively. Thease allergens newly added to inhalant and food panels were not contributable to the detection of specifical allergen. We needs further improvement to be used as primary screening or quantitative test for allergy.

• 대한한방소아과학회지
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• 제21권2호
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• pp.169-184
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• 2007

Objetives The late research tendency on atopic dermatitis is mainly focused on the experiment of unbalance of cytokine released from T cell subtype. Merhods As there's no experiment with herbal medicine using Hanghaedan(HHD), we isolated and cultured monocytes of atopic dermatitis pateints (AD) peripheral blood, for knowing the influence of HHD on the secretory function of IL-4, IL-5, $IFN-{\gamma}$. Results 1. In AD group, HHD exhibits statistically significant inhibitory effect on IL-4(p<0.05). 2. In AD group, HHD exhibits statistically significant inhibitory effect on IL-5(p<0.05). 3. In AD group, HHD exhibits statistically significant inhibitory effect on $IFN-{\gamma}$ (p<0.01). 4. Sample group comparing with the cytokine concentration rate value of AD patients and that of NAD patients IL-4 of AD patients exhibits statistically significant decrease(p<0.05). 5. In AD group, there is no significant relation between serum IgE and cytokines. 6. In AD group, there is no significant relation between total eosinophil and IL-5. Conclusions Effects of Hanghaedan on the cytokine(IL-4, IL-5, $IFN-{\gamma}$) secretory function of monocytes of atopic dermatitis patients is recognized.

• 대한한방소아과학회지
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• 제15권2호
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• pp.177-188
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• 2001

Background : Atopic Dermatitis is thought to be a common and chronic relapsing inflammatory skin disease that probably results from allergic reaction. Because it make some serious problems in children, it is needed to treat and at least subside symptoms. Objective : The aim of this study was to evaluate the efficacy of GamiYeoldahansotang(加味熱多寒少湯) for treating Atopic Dermatitis and survey the general characteristics in children with Atopic Dermatitis. Method : Atopic Dermatitis with typical clinical symptoms were included in this study. Forty two patients were treated with hot water extract of GamiYeoldahansotang(加味熱多寒少湯) for four weeks. Clinical evaluation were made by Jacob T scoring system before and after treatment. Total Serum IgE, Eosinophil count were also conducted. Results : Reduction in body surface area was not observed. But significant reductions of severity scores before and after adminstration of GamiYeoldahansotang(加味熱多寒少湯) were observed in all of three groups: mind, moderate, severe. specially severe intensity group was very statistically significant.(p<0.05) Conclusion : We speculate that GamiYeoldahansotang(加味熱多寒少湯) has some therapeutic effects in mitigating the symptoms of Atopic Dermatitis.

• 한방안이비인후피부과학회지
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• 제22권2호
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• pp.223-237
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• 2009

본 임상연구는 임상연구 모집 공고를 통하여 모집된 대상자 중 Atopic Dermatitis Research Group (2005) 한국인 아토피 피부염 진단기준에 따라 아토피 피부염으로 판정되고 현재 약물(한약, 양약)치료나 외용제 치료를 하고 있지 않은 만 5$\sim$50세의 아토피 피부염 환자를 대상으로 하였다. 피험자들을 이중맹검, 블록무작위배정하여 시험군과 대조군으로 나누어 6주간의 시험기간 동안 육안적 평가, 혈액학적 검사, 피부측정 장비를 통한 기기적 평가를 시험 전후에 실시하여, 시험군에 사용된 제품이 대조군 사용된 제품에 비하여 얼마나 효과적으로 아토피 피부염으로 손상된 피부 장벽을 회복하는지 관찰하였다. 본 임상시험을 통해 'Atomento solution & cream'이 손상된 피부장벽을 회복시키고 피부 보습력을 증가시켜 아토피 피부염에 효과적이었으며, 인체에 이상반응이나 부작용 없이 안전하게 사용될 수 있음을 확인하였다.

• 한국임상약학회지
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• 제22권3호
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• pp.268-273
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• 2012

The definition of anaphylaxis is 'a serious, life-threatening generalized or systemic hypersensitivity reaction' and is considered as the life threatening adverse drug reaction. We experienced a case of cefotetan induced anaphylaxis with negative pre-skin test, used for surgical prophylaxis. A 82-year-old female was scheduled for total knee replacement therapy. She had no previous history of allergy and her skin test results were also negative. On her right knee surgery, she underwent cefotetan therapy as a surgical prophylaxis for a week with no problems identified. Next left knee surgery, she also received the prophylaxis of intravenous cefotetan. However, a few minutes later, anaphylactic reaction developed with vomiting, severe hypotension, bronchospasm, and dyspnea. After immediate intensive care treatment, she recovered without significant complications. Though commonly used laboratory data in case reports, such as the specific IgE, tryptase, histamine, or allergic skin prick test were limited, we successfully confirmed anaphylaxis based on clinical criteria for diagnosing anaphylaxis based on WAO 2011 guideline with through concurrent patient°Øs medical history review and the process of identifying the causes.

• Parasites, Hosts and Diseases
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• 제44권1호
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• pp.81-86
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• 2006

Anisakiasis is a parasitic disease caused by ingestion of raw fish infected with anisakid larvae. Endoscopic changing patterns of submucosal lesions in chronic gastric anisakiasis have not been known yet. Here we report 4 cases of suspected gastric anisakiasis which were improved during follow-up periods without surgical treatment. The patients presented with abdominal pain, nausea and vomiting after consuming raw marine fish, and visited our gastroenterology outpatient department. Their endoscopic findings showed firm and yellowish submucosal masses accompanied with eccentric erosions. Histologic findings showed severe eosinophilic infiltrations. In blood tests, peripheral eosinophil counts and total IgE levels were elevated. We believed that all cases were caused by larval anisakid infections. The submucosal mass lesions disappeared during the follow-up periods of 2 to 4 mo.