• Proceedings of the Korean Society of Plant Pathology Conference
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• 1999.04a
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• pp.29-29
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• 1999

• Life and Agrochemicals
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• s.283
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• pp.42-44
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• 2012

우리나라 파프리카에 발생하는 주요 바이러스는 오이모자이크바이러스(Cucumber mosaic virus, CMV), 고추모틀바이러스(Pepper mottle virus, PepMoV), 잠두위조바이러스2(Broad bean wilt virus2, BBWV2), 고추약한모틀바이러스(Pepper mild mottle virus, PMMoV), 토마토반점위조바이러스(Tomato spotted wilt virus, TSWV) 및 사탕무황화바이러스(Beet western yellows virus, BWYV) 등 6종이다. 생산량 감소 및 고품질 규격품 생산의 가장 큰 제한요인이 되고 있는 바이러스병의 피해를 최소화하기 하기 위하여 바이러스별 병징 및 발생원인에 따른 예방법을 알아본다.

• Korean Journal of Organic Agriculture
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• v.22 no.4
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• pp.657-665
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• 2014

This study was carried out to study the effect of eggplant (EG203) grafting and strawberry removal time on succeeding tomato cropping in high temperature season. In non tillage culture, bacterial wilt incidence was 30% and 0% in nongrafting and eggplant (EG203) grafting while 25% in non-grafting and 0% in eggplant (EG203) grafting in tillage culture. Commercial yield was similar in tillage and non-tillage culture using EG-203 grafting as 2,693 kg/10a and 2,657 kg/10a respectively with no difference in soluble solid content and hardness. Strawberry removal time after tomato planting enhanced plant height and internode length in 20days and 30days later removal while no difference in other growth characters. Commercial yield was higher as 1,885 kg/10a in strawberry removal before tomato planting than 1,678 kg/10a in 30days later removal. From this research, strawberry removal time should be determined by considering strawberry price and growth and expecting profit by succeeding tomato cropping.

• Korean Journal of Environmental Biology
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• v.39 no.3
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• pp.319-328
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• 2021

Tomato spotted wilt virus (TSWV) is one of the most destructive viruses worldwide, which causes severe damage to economically important crops, such as pepper and tomato. In this study, we examined the molecular and biological characterization of a TSWV isolate (SW-TO2) infecting tomato and compared it to the recently reported isolates from boxthorn, butterbur, and angelica plants. The phylogenetic analysis based on the complete genome sequences confirmed that SW-TO2 was clustered with those of isolates from boxthorn and pepper in Korea with the maximum nucleotide identities ranging from 98% to 99%. We developed the bioassay method for screening TSWV resistance and tested some commercial pepper and tomato cultivars for resistance evaluation of four isolates of TSWV. TSWV resistance was evaluated as TSWV resistance when all the following three conditions were satisfied: first, when symptoms of necrotic spots or no symptoms were present in the inoculated leaves; second, when there were no symptoms in the upper leaves; and third, when the upper leaves were negative as a result of RT-PCR diagnosis.

• Research in Plant Disease
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• v.11 no.2
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• pp.193-197
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• 2005

The effect of pH on the survival of R. solanacearum and its transmission via roots of tomato in hydroponic culture were studied in laboratory and greenhouse. In laboratory experiment, R. solanacearum could not survive for 24h in nutrient solution with pH of $4{\cdot}0;or\;4{\cdot}5$, while 1, 14, 51 and $62\%$ of inoculum survived at pH $5{\cdot}0,\;5{\cdot}6\;and\;6{\cdot}5$, respectively. When tomato plants were inoculated with R. solanacearum through wounds on the stems, the bacteria moved downward from the inoculation site to the roots and infectious bacteria were released from the roots into the nutrient solution. Of two pH regimes tested in greenhouse nutrient-film technique(NFT) culture, the R. solanacearum population was significantly lower in pH 5.0 than in pH 6.5 in most sampling data. In treatments in which R. solanacearum was introduced by transplanting two root-inoculated plants, significantly move plants developed wilt at pH $6{\cdot}5$(34 out of 48 plants) than at pH 5.0(11 out of 48 plants). In addition, when the bacterium was introduced by transplanting two stem-inoculated plants at pH $6{\cdot}5$, seven out of 24 plants developed wilt.

• Research in Plant Disease
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• v.28 no.4
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• pp.231-236
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• 2022

Oxypetalum coeruleum, commonly known as Tweedia, is a perennial herbaceous plant of the Apocynaceae family native to southern Brazil and Uruguay. Tweedia plants are grown as one of the most popular ornamental flowers for floral arrangement in Korea. In May 2021, several tweedia plants in a single greenhouse in Gimje, Jeollabuk-do were found to show virus-like symptoms including necrotic rings, vein-clearing, chlorotic mottle, and mosaic on the leaves, and necrosis on the stems. Here, we have identified tomato spotted wilt virus (TSWV) in symptomatic tweedia leaves by applying high-throughput RNA sequencing. In the result, a single infection by TSWV was verified without mixed infections of different virus species. To confirm the presence of TSWV, a reverse transcription polymerase chain reaction was performed with a specific primer set to the N gene of TSWV. The complete genomic sequence of L, M, and S segments of TSWV 'Oxy' isolate were determined and deposited in GenBank under accession numbers LC671525, LC671638, and LC671639, respectively. In the phylogenetic tree analysis by maximum likelihood method, 'Oxy' isolate showed a high relationship with TSWV 'Gumi' isolate from Gerbera jamesonii in Gyeongsangbuk-do, Korea; for all three RNA segments. To our knowledge, this is the first report of TSWV infection of O. coeruleum in Korea.

• Research in Plant Disease
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• v.23 no.2
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• pp.193-201
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• 2017

Major tomato viruses in Korea are Tomato chlorosis virus (ToCV), Tomato spotted wilt virus (TSWV), Cucumber mosaic virus (CMV), Pepper mottle virus (PepMoV), and Tomato mosaic virus (ToMV). RT-PCR conditions for the viruses were examined, especially in primer set and RT-PCR mixture. Total 46 primer sets from the unique sequence of the viruses were tested for nonspecific background products in a RT-PCR mixture without template. Among them 16 primer sets were applied to healthy tomato RNA, resulting the compatibility between RT-PCR mixture and primer set influenced RT-PCR to reduce nonspecific background products. Based on the combinations among cDNA synthesis parameters and RT-PCR mixtures, two reaction mixtures were finally selected for ToCV detection. The condition allowed to determine more specific primer sets; C029 (ToCV), C072 (TSWV), C070 (CMV), C048 (PepMoV), and C065 (ToMV). These primer sets are expected to be of use to specific detection of the major viruses in tomato plants.

• Journal of Bio-Environment Control
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• v.28 no.3
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• pp.212-217
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• 2019

Bacterial wilt caused by Ralstonia solanacearum is a major disease that affects tomato plants widely. R. solanacearum is a soil born pathogen which limits the disease control measures. Therefore, breeding of resistant tomato variety to this disease is important. To identify the susceptible variety, degree of disease resistance has to be determined. In this study, micro sap flow sensor is used for accurate prediction of resistant degree. The sensor is designed to measure sap flow and water use in stems of plants. Using this sensor, the susceptibility to bacterial wilt disease can be identified two to three days prior to the onsite of symptoms after innoculation of R. solanacearum. Thus, this find of diagnosis approach can be utilized for the early detection of bacterial wilt disease.

• The Plant Pathology Journal
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• v.34 no.1
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• pp.23-34
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• 2018

The Ralstonia solanacearum species complex (RSSC) can be divided into four phylotypes, and includes phenotypically diverse bacterial strains that cause bacterial wilt on various host plants. This study used 93 RSSC isolates responsible for potato bacterial wilt in Korea, and investigated their phylogenetic relatedness based on the analysis of phylotype, biovar, and host range. Of the 93 isolates, twenty-two were identified as biovar 2, eight as biovar 3, and sixty-three as biovar 4. Applied to the phylotype scheme, biovar 3 and 4 isolates belonged to phylotype I, and biovar 2 isolates belonged to phylotype IV. This classification was consistent with phylogenetic trees based on 16S rRNA and egl gene sequences, in which biovar 3 and 4 isolates clustered to phylotype I, and biovar 2 isolates clustered to phylotype IV. Korean biovar 2 isolates were distinct from biovar 3 and 4 isolates pathologically as well as genetically - all biovar 2 isolates were nonpathogenic to peppers. Additionally, in host-determining assays, we found uncommon strains among biovar 2 of phylotype IV, which were the tomato-nonpathogenic strains. Since tomatoes are known to be highly susceptible to RSSC, to the best of our knowledge this is the first report of tomato-nonpathogenic potato strains. These results imply the potential prevalence of greater RSSC diversity in terms of host range than would be predicted based on phylogenetic analysis.

• The Plant Pathology Journal
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• v.39 no.1
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• pp.108-122
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• 2023

Fusarium oxysporum f. sp. lycopersici (Fol) and Fusarium oxysporum f. sp. cubense (Foc), are the causal agent of Fusarium wilt disease of tomato and banana, respectively, and cause significant yield losses worldwide. A cost-effective measure, such as biological control agents, was used as an alternative method to control these pathogens. Therefore, in this study, six isolates of the Streptomyces-like colony were isolated from soils and their antagonistic activity against phytopathogenic fungi and plant growth-promoting (PGP) activity were assessed. The results showed that these isolates could inhibit the mycelial growth of Fol and Foc. Among them, isolate STRM304 showed the highest percentage of mycelial growth reduction and broad-spectrum antagonistic activity against all tested fungi. In the pot experiment study, the culture filtrate of isolates STRM103 and STRM104 significantly decreased disease severity and symptoms in Fol inoculated plants. Similarly, the culture filtrate of the STRM304 isolate significantly reduced the severity of the disease and symptoms of the disease in Foc inoculated plants. The PGP activity test presents PGP activities, such as indole acetic acid production, phosphate solubilization, starch hydrolysis, lignin hydrolysis, and cellulase activity. Interestingly, the application of the culture filtrate from all isolates increased the percentage of tomato seed germination and stimulated the growth of tomato plants and banana seedlings, increasing the elongation of the shoot and the root and shoot and root weight compared to the control treatment. Therefore, the isolate STRM103 and STRM104, and STRM304 could be used as biocontrol and PGP agents for tomato and banana, respectively, in sustainable agriculture.