• Title/Summary/Keyword: tomato leaves

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Detection of TMV, ToMV, and CMV from Tomato Seeds and Plants (토마토 종자와 식물체로부터의 TMV, ToMV 및 CMV 검출)

  • Park, Kyung-Hoon;Cha, Byeong-Jin
    • Research in Plant Disease
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    • v.8 no.2
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    • pp.101-106
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    • 2002
  • For the detection of Tobacco masaic virus (TMV), Tomato mosaic virus (ToMV), and Cucumber mosaic virus (CMV), tomato seeds of 11 table tomato and 7 cherry tomato cultivars were assayed by DAS-ELISA. Among the cultivars, TMV and ToMV were detected from 9 cultivars at the rates lower than 20% and 16%, respectively. In the assay on seed transmission rates, ToMV and CMV were detected as high as 24% and 8% , respectively, but TMV was not detected. In field survey on these viruses from tomato plants of 10 different places in Chungbuk province, ToMV and CMV were detected from most fields. TMV was detected from only 3 fields. The highest detection rates of these viruses were recorded in Cheongwon for TMV Chungju for ToMV, and the other locality of Chungju far CMV. It was difficult to find any relationship between the growth stage of tomato and infection rates. TMV usually caused mosaic on leaves while ToMV caused various symptoms including yellows, necrosis, and mottling. CMV-infected tomato plants showed symptoms of shoestring, fern leaf, and yellows.

Effect of Intercropping Ratio on the Cherry Tomato with Basil on the Growth, Physiological, and Productivity Parameters on the Rooftop in Urban Agriculture (옥상 도시농업에서 방울토마토(Lycopersicon esculentum)와 바질(Ocimum basilicum)간의 공영식재가 생육, 생리, 생산성에 미치는 영향)

  • Ju, Jin-Hee;Song, Hee-Yeon;Oh, Deuk-Kyun;Park, Sun-Yeong;Yoon, Yong-Han
    • Journal of Environmental Science International
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    • v.30 no.9
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    • pp.709-717
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    • 2021
  • This study evaluated the growth, physiological responses and productivity based on the intercropping ratio of cherry tomato (Lycopersicon esculentum L.) with basil (Ocimum basilicum L.). on the rooftops to determine out the efficient ratio in urban agriculture. From April to September 2019, an experiment was conducted on the rooftop of Konkuk University Glocal Campus. Cherry tomato and basil were selected as companion plants for eco-friendly urban agriculture on the rooftops. Each plot was created with a width of 100 cm, length of 100 cm, and height of 25 cm. After installing drainage and waterproof layers from bottom to top, substrate was laid out with a height of 20 cm. Intercropping ratio was consisted of a single tomato plant (TC), 2:1 tomato to basil (T2B1), 1:1 tomato to basil (T1B1), 1:2 tomato to basil 2 (T1B2), and a single basil plant (BC), were conducted using a randomized complete plot design with five treatments and three replication (a total 15 plots). Measurements were divided into growth, physiological responses, and productivity parameters, and detailed items were investigated and analyzed by classifying them into plant height, leaf length, leaf width, number of leaves, root length, root collar caliper, chlorophyll contents, fresh weight, dry weight, number of fruit, fruit caliper, fruit weight, and sugar content. Comparative analyses of cherry tomato with basil plants by intercropping ratio, growth, physiological, and productivity responses are determined to be efficient when the ratio of cherry tomato to basil ratio is 2:1 or 1:1.

Activation of Defense Responses in Chinese Cabbage by a Nonhost Pathogen, Pseudomonas syringae pv. tomato

  • Park, Yong-Soon;Jeon, Myeong-Hoon;Lee, Sung-Hee;Moon, Jee-Sook;Cha, Jae-Soon;Kim, Hak-Yong;Cho, Tae-Ju
    • BMB Reports
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    • v.38 no.6
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    • pp.748-754
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    • 2005
  • Pseudomonas syringae pv. tomato (Pst) causes a bacterial speck disease in tomato and Arabidopsis. In Chinese cabbage, in which host-pathogen interactions are not well understood, Pst does not cause disease but rather elicits a hypersensitive response. Pst induces localized cell death and $H_2O_2$ accumulation, a typical hypersensitive response, in infiltrated cabbage leaves. Pre-inoculation with Pst was found to induce resistance to Erwinia carotovora subsp. carotovora, a pathogen that causes soft rot disease in Chinese cabbage. An examination of the expression profiles of 12 previously identified Pst-inducible genes revealed that the majority of these genes were activated by salicylic acid or BTH; however, expressions of the genes encoding PR4 and a class IV chitinase were induced by ethephon, an ethylene-releasing compound, but not by salicylic acid, BTH, or methyl jasmonate. This implies that Pst activates both salicylate-dependent and salicylate-independent defense responses in Chinese cabbage.

Occurrence of Target Spot of Tomato Caused by Corynespora cassiicola in Korea (Corynespora cassiicola에 의한 토마토 갈색무늬병(가칭)의 발생)

  • Kim, Jong Tae;Yu, Seung Hun;Yun, Hae Keun
    • Korean Journal of Agricultural Science
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    • v.17 no.2
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    • pp.82-86
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    • 1990
  • A Corynespora species was isolated from brown target spots developed on leaves of tomato. The fungus was identified as Corynespora cassiicola and proved to be pathogenic to tomato plants. Growth of the fungus in vitro was optimum at $27^{\circ}C$, and poor at 19 and $35^{\circ}C$. Sporulation was abundant on potato dextrose malt agar and moderate on V-8 juice agar, potato dextrose agar and Czapek agar.

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Occurrence of Tomato spotted wilt virus in Chrysanthemum (Dendranthema grandiflorum) in Korea

  • Chung Bong-Nam;Pak Ha-Seung;Jung Jae-A;Kim Jeong-Soo
    • The Plant Pathology Journal
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    • v.22 no.3
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    • pp.230-234
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    • 2006
  • Tomato spotted wilt virus (TSWV) has been identified in commercial chrysanthemum cultivars in Korea. Nucleotide sequences of the N gene of TSWV-ch14 isolated from infected chrysanthemum were determined and deposited in GenBank under accession no. DQ453158. The symptoms consisted of dark colored leaf necrosis, black streaks along the stem, wilting of plant parts in 'Sinma'; and chlorotic spots, necrosis of axillary shoots and withering of leaves in 'Hwarang'. Electron micrographs of leaf preparation of Nicotiana rustica infected with TSWV-ch14 contained spherical particles around 85 nm in diameter. TSWV was identified from chrysanthemum by sequence determination of N nucleocapsid protein and virion observation by transmission electron microscope. This is the first reported observation on TSWV in chrysanthemum in Korea.

Agrobacterium-mediated transformation of Lycopersicon esculentum (cv. MicroTom) with two pathogen-induced hot pepper transcription factors

  • Seong, Eun-Soo;Oh, Sang-Keun;Eunsook Chung;Park, Doil
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.79.1-79
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    • 2003
  • Two pathogen-induced hot pepper transcription factors (CaNACl and CapIfl) were introduced into‘MicroTom’tomato by Agrobacterium tumefaciens-mediated transformation. We used to nptII containing kanamycin resistance gene as a selection marker. Both transformed and non-transformed plants were transferred to pot after rooting test in vitro. To approximate the levels of caNACl transcript in leaves of wild-type and transgenic plants, RNA blots were hybridized with double-stranded full-length CaNACl probe at moderate stringency, Although the relative signal strength for hybridization fluctuated among the samples on different blots, transgenic plant lines N-1, N-2 and N-3 consistently displayed increased levels of CaNACl transcript relative to other transgenic lines and wild-type plants. Of all the transgenic lines examined, line N-7 had the least amount of CaNACl transcript. Role of these transcription factors in pathogen defense will be examined by overexpression in tomato.

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Cadmium-Induced Phytotoxicity in Tomato Seedlings Due to the Accumulation of H2O2 That Results from the Reduced Activities of H2O2 Detoxifying Enzymes

  • Cho, Un-Haing
    • The Korean Journal of Ecology
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    • v.27 no.2
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    • pp.107-114
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    • 2004
  • Tomato (Lycopersicon esculentum) seedlings exposed to various concentrations of $CdC1_2$ (0∼100 $\mu$M) in the nutrient solution for up to 9 days were analyzed with the seedling growth, $H_2O_2$ production, glutathione levels and activity changes of enzymes related to $H_2O_2$ removal. The growth of seedlings was inhibited with over 50 $\mu$M Cd, whereas the levels of $H_2O_2$ and glutathione were enhanced with Cd exposure level and time. Meanwhile, Cd exposure increased the activities of catalase (CAT) and glutathione reductase (GR) but decreased the activities of dehydroascorbate acid reductase (DHAR) and ascorbate peroxidase (APX) in both leaves and roots. These results suggest that the altered activities of antioxidant enzymes particularly involved in the $H_2O_2$ removal and the subsequent $H_2O$$_2$ accumulation could induce the Cd-induced phytotoxicity.

Nucleotide Sequence of a Proteinase Inhibitor I Gene in Potato (감자에 존재하는 단백질분해효소 억제제 I 유전자의 염기서열)

  • 이종섭
    • Journal of Plant Biology
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    • v.32 no.2
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    • pp.67-78
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    • 1989
  • Hybridization of DNA isolated from leaves of Russet Burbank potato with tomato cDNA as a probe revealed the presence of about ten inhibitor 1 genes in the genome. Screening of a genomic library of Russet Burbank potato resulted in isolation of seven different genomic clones carrying inhibitor I genes. One of the genomic clones, clone 2, contained two EcoRI fragments of 3.4 and 1.8 kb in size, respectively, which were hybridized with the probe. The nucleotide sequence of parts of the hybridizing EcoRI fragments revealed that they contain a complete gene which codes for an open reading frame of 107 amino acids. It is interrupted by two intervening sequences of 502 and 493 bp, situated at the positions of codons 17 and 43, respectively, of the open reading frame. Putative regulatory sequences, TATAAA and CCACT, were found at the 5' flanking region. In addition, a copy of a 100 bp repeat found at a tomato inhibitor I gene was identified.

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Effect of Host Plants on the Development and Reproduction of Frankliniella occidentalis (Pergande) (Thysanoptera : Thripidae) (기주식물이 꽃노랑총채벌레(Frankliniella occidentalis)의 발육과 생식에 미치는 영향)

  • 이건휘;백채훈;황창연;최만영;김두호;나승용;김상수;최인후
    • Korean journal of applied entomology
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    • v.42 no.4
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    • pp.301-305
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    • 2003
  • This study investigated the ecological characteristics of Frankliniella occidentalis on four horticultural crops. On red pepper leaves, larval developmental period and survival rate of F. occidentalis were 6.4 days and 67%, respectively. While adult female lived longer on cucumber leaves as long as 16 days, on Petal lived 8.1 days. F. occidentalis adult female preferred tomato to eggplant, cucumber and red pepper, and higher number of hatched larva were from leaves of middle part of egg-plant compared with those from other parts. F. occidentalis adult female fed preferably on petal compared with leaves and Petals of eggplant, and among petals of four tested plants, the most preference was those of red pepper.

Selection of Compound Leaf Position for Sap Test of Hydroponic Tomato and Relationship between Inorganic Elements of Sap and Dried Matter (양액재배 토마토의 즙액검정을 위한 엽위 선정과 즙액 및 건물체의 무기원소 상관 관계)

  • Kim, Yang-Rok;Kang, Tae-Woo;Song, Sung-Jun;U., Zang-Kual
    • Korean Journal of Soil Science and Fertilizer
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    • v.36 no.6
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    • pp.391-398
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    • 2003
  • Research was conducted to establish the leaf and petiole sap test to diagnose the nutritional status of tomato. The concentrations of $NO_3$, $PO_4$, K, Ca, Mg and $SO_4$ ions extracted from the leaves and petioles in the different positions in a plant were measured and compared with their corresponding inorganic contents in the leaves analyzed by the chemical method. The ionic concentrations in the leaf and petiole sap showed the different values depending on their positions in a plant. The leaves and petioles in the lower positions of a plant had higher concentrations of $NO_3$, Ca, Mg and $SO_4$ ions. In particular, there were greater changes of ionic concentrations and less increases in the leaf length and width from the 9th compound leaf down from the uppermost cluster. On the other hand, the leaflets in a compound leaf had the same ionic concentrations. Therefore, it appeared that the optimal sampling position of leaf and petiole for the sap test is the leaflets of the 9th compound leaf down from the uppermost cluster. A good correlation between the sap test and the chemical analysis of plant showed that the ionic concentrations in the leaf and petiole sap reflect the nutritional status of tomatoes.