• Title/Summary/Keyword: tomato disease

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Research on composting of sewage sludge using dryer facility with indirect heating system (간접가온 건조방식의 건조시설을 이용한 하수슬러지 퇴비화에 관한 연구)

  • Baik, Seon-Jai;Han, In-Sup;Choi, Ick-Hoon;Kang, Sung-Hyo;Hong, Seong-Min
    • Journal of Korean Society of Water and Wastewater
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    • v.28 no.3
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    • pp.299-304
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    • 2014
  • The need of reusing sewage sludge is highly increasing as the amount of domestic sewage sludge is growing and sewage management strategies were reinforced. Accordingly, in this research, we studied the composting possibility of sludge in sewage treatment plant J, using dryer facility with indirect system by conducting component analysis of dried sludge and experiment on cultivating crops. It was observed from the component analysis, that the dried sludge is appropriate for both decomposed manure standard of Ministry of Environment and fertilizer standard of Rural Development Administration. Besides, in the experiment on cultivating lettuce, the experimental group(soil + dried sludge) was superior to the control group(soil + bed soil) in apparent condition of leaves tensity and damage by disease and pest. In case of cultivating tomato, the experimental group showed stronger durability of fruits dangling on stems compared to the control group after 9 weeks. Consequently, the excess sludge dried by indirect heating system in the sewage treatment plant J is appropriate for the standard of both Ministry of Environment and Rural Development Administration and show good result in experiment on cultivating crops therefore it can be reused for composting.

Risk Assessment of Genetically Modified Organisms (유전자변형 생물체의 위해성평가)

  • 김형진;김환묵
    • Toxicological Research
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    • v.19 no.1
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    • pp.1-12
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    • 2003
  • New breeding method by genetic engineering is expected as a key technology to solve food shortage due to the growing world population in the year 2000s. Many genetically modified organisms (GMOs) were already developed and the commercial cultivation had started. The first GMO, Flavr Savr tomato, which rotted at a much slower pace than ordinary ones, was developed in US in 1994. Since then, over than 70 different agricultural products including corn, cotton, soybean, papaya, potato, and squash made with genetically modified plants are reportedly on sale worldwide. Supporters favor the GMOs because they have greater yields, longer shelf lives and stronger resistance to disease and insects. On the other hand, opponents say that the supporters ignore a potential danger that they may damage the environment as well as human beings. To assure the safe development and use of GMOs as food and other biotech products, the possible risks on biological environment and human health should be throughly examined and regulated by developer and government. Because the biosafety problem is a global, environmental, and trade issue, a new international treaty is under development. The Cartagena Protocol on Biosafety was adopted at the 1 st Extraordinary Conference of Parties of the Convention on Biological Diversity which was held at Mont-real, Canada, Jan. 29th, 2000. The adoption of the Protocol is seen as a breakthrough in that it is based on the" Precautionary Principle" despite scientific uncertainties surrounding potential risks that GMOs may inflict on human health and the environment and that it has laid the ground for introduction of specific steps to handle international trading of GMOs. In this paper, the authors would like to introduce the current status and perspective of environmental and human risk assessment of GMOs.t of GMOs.

Selection of Antagonistic Bacteria for Biocontrol of Botrytis cinerea Causing Gray Mold on Vitis spp (포도 잿빛곰팡이병의 생물적 방제를 위한 길항세균 선발)

  • Seo, Sang-Tae;Park, Jong-Han;Han, Kyoung-Suk;Cheong, Seung-Ryong
    • Research in Plant Disease
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    • v.12 no.3
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    • pp.267-271
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    • 2006
  • Botrytis cinerea Pers. was found to be highly virulent to the grapevine plant, especially in greenhouse condition. Pseudomonas species play key roles for the biocontrol of many plant diseases especially in soil. Of the 83 isolates of Pseudomonas spp., a bacterial strain P84, isolated from tomato rhizosphere, was shown to suppress a wide range of phytopathogenic fungi in vitro. The isolate was identified as Pseudomonas putida on the basis of its bacteriological and genetic characteristics. The P. putida P84 strain carry the phlD gene for 2,4-diacetylphloroglucinol biosynthesis and may produce the antibiotics as an antagonistic mechanism involved in biocontrol. The antagonistic activity of the bacterium has a promising implication for its use as a biocontrol agent to control grapevine gray mold.

Synthesis of 2(5H)-Furanones and 2-Cycloalkenones and Their Fungicidal Activities (2(5H)-Furanone 및 2-Cycloalkenone 유도체의 합성과 살균활성)

  • Heo, Jung-Nyoung;Song, Young-Seob;Park, No-Kyun;Choi, Gyung-Ja;Jang, Kyung-Soo;Kim, Bum-Tae
    • The Korean Journal of Pesticide Science
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    • v.11 no.1
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    • pp.1-7
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    • 2007
  • We have developed an efficient synthesis of 2(5H)-furanones using the microwave-promoted Suzuki-Miyaura coupling reaction and evaluated their fungicidal activities against six fungal pathogens in vivo. In addition, 2-cyclopentenones or 2-cyclohexenones possessing one or two additional methylene groups instead of the oxygen in the 2(5H)-furanone skeleton were also prepared by using the similar method. As the results of in vivo fungicidal screening against 6 plant diseases, a few derivatives displayed specific fungicidal activities against rice blast and tomato late blast. Further studies toward the optimization of the chemical structures are necessary for the development of novel fungicides with high potency.

Development of RT-PCR and Nested PCR for Detecting Four Quarantine Plant Viruses Belonging to Nepovirus (RT-PCR과 nested PCR을 이용한 Nepovirus속 식물검역 바이러스 4종의 정밀진단)

  • Lee, Siwon;Kang, Eun-Ha;Shin, Yong-Gil;Lee, Su-Heon
    • Research in Plant Disease
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    • v.19 no.3
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    • pp.220-225
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    • 2013
  • For quarantine purpose, we developed the RT- and nested PCR module of Tomato black ring virus (TBRV), Arabis mosaic virus (ArMV), Cherry leafroll virus (CLRV) and Grapevine fanleaf virus (GFLV). The PCR modules, developed in this study make diagnosis more convenient and speedy because of same PCR condition. And also, the methods are more accurate because it can check whether the result is contamination or not using the mutation-positive control. We discard or return the 27 cases of Nepovirus infection seed by employing the module past 3 years. This study provides a rapid and useful method for detection of four quarantine plant viruses.

Phytophthora citricola, a Causal Agent of Jujube (Zizyphus jujuba) Fruit Rot (대추 역병균의 Phytophthora citricola의 동정)

  • 지형진;임양숙;정기채;조원대
    • Korean Journal Plant Pathology
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    • v.14 no.5
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    • pp.402-407
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    • 1998
  • Phytophthora rot on jujube fruit has occurred at several cultivation areas in Kyung-buk and Kyung-nam provinces. Symptoms consisted of brownish to reddish rot on fruits resulting in early drop or mummification. The causal fungus isolated from infected fruits and adjacent leaf stalks was identified as Phytophthora citricola, which has never been reported in Korea. Sporangia were semi-papillate, noncaducous and highly variable in shapes. Plerotic oospores with paragynous antheridia were abundant is single cultures. Sporangia of two isolates were measured as 38-76$\times$20-40 ${\mu}{\textrm}{m}$ and averaged 51.4$\times$27.0 and 55.6$\times$36.0 ${\mu}{\textrm}{m}$. Oogonia were ranged from 26 to 36 ${\mu}{\textrm}{m}$ and averaged 31.3 and 32.0 ${\mu}{\textrm}{m}$. Colony pattern was slightly radiated with sparse aerial mycelia on common media. Minium, optimum and maximum temperatures for mycelial growth were recorded at 7, 25, and 32$^{\circ}C$, respectively. Among tested media, 10% V8A was the best and $25^{\circ}C$ was better than 15$^{\circ}C$ for oospore formation of the fungus. The jujube isolates of P. citricola were readily differentiated from other closely related species in the genus, namely; P. nicotianae, P. citrophthora, P. cactorum, P. capsici, and P. plalmivora on the basis of PCR-RFLP of r-DNA. The fungus showed strong pathogenicty to jujube, apple, pear, orange, persimmon and eggplant, and relatively weak to citron, tomato, pepper and cucumber. In this study, P. citrocola is firstly identified and jujube fruit rot caused by the fungus is recorded as a new disease in Korea.

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Environmental Factors Affecting Parasitism to Cucumber Powdery Mildew Fungus, Sphaerotheca fusca by Ampelomyces quisqualis 94013 and Its Host Range (Ampelomyces quisqualis 94013의 오이 흰가루병균 기생에 영향을 미치는 환경조건과 기주범위)

  • Lee, Sang-Yeob;Ryu, Jae-Dang;Kim, Hong-Gi
    • Research in Plant Disease
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    • v.11 no.2
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    • pp.167-172
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    • 2005
  • An isolate of Ampelomyces quisqualis 94013 was selected as an effective parasite for biological control against cucumber powdery mildew. Temperature range for the parasitism of A. quisqualis 94013 against cucumber powdery mildew was $12\~30^{\circ}C$, and optimal temperature for that $20\~28^{\circ}C$. In $20\~35\%$ humidity of the greenhouse, parasitic ability of A. quisqualis 94013 against Sphaerotheca fusca was not good. Inoculation tests revealed that A. quisqualis 94013 can parasitize on six species of Sphaerotheca in the 12 crops and Ersiphe cichoracerum in tomato. As host rang of A. quisqualis 94013 was broad and it may be used effectively as a biocontrol agent for powdery mildew of 13 crops.

Proteome Analysis of Disease Resistance against Ralstonia solanacearum in Potato Cultivar CT206-10

  • Park, Sangryeol;Gupta, Ravi;Krishna, R.;Kim, Sun Tae;Lee, Dong Yeol;Hwang, Duk-ju;Bae, Shin-Chul;Ahn, Il-Pyung
    • The Plant Pathology Journal
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    • v.32 no.1
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    • pp.25-32
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    • 2016
  • Potato is one of the most important crops worldwide. Its commercial cultivars are highly susceptible to many fungal and bacterial diseases. Among these, bacterial wilt caused by Ralstonia solanacearum causes significant yield loss. In the present study, integrated proteomics and genomics approaches were used in order to identify bacterial wilt resistant genes from Rs resistance potato cultivar CT-206-10. 2-DE and MALDI-TOF/TOF-MS analysis identified eight differentially abundant proteins including glycine-rich RNA binding protein (GRP), tomato stress induced-1 (TSI-1) protein, pathogenesis-related (STH-2) protein and pentatricopeptide repeat containing (PPR) protein in response to Rs infection. Further, semi-quantitative RT-PCR identified up-regulation in transcript levels of all these genes upon Rs infection. Taken together, our results showed the involvement of the identified proteins in the Rs stress tolerance in potato. In the future, it would be interesting to raise the transgenic plants to further validate their involvement in resistance against Rs in potato.

Nutrient Intake and Dietary Behaviors of Patients with Functional Dyspepsia (기능성 소화불량증 환자의 영양소 섭취 및 식행동)

  • Shin, Ji-Won;Lee, Geum-Ju;Park, Jae-Woo
    • Journal of the Korean Dietetic Association
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    • v.18 no.4
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    • pp.283-296
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    • 2012
  • As a disease that reduces quality of life, functional dyspepsia (FD) is associated with foods that may worsen its symptoms or cause it. The purpose of this study was to examine the nutritional status and dietary behaviors of FD patients. We investigated food intake, food intake frequency, and dietary habits of 45 FD subjects according to the Rome III Diagnosis Criteria. Average age and body mass index (BMI) were 47.7 years and $22.6kg/m^2$ (males: $23.4kg/m^2$, females: $22.1kg/m^2$), respectively. Average energy intake was 77% of Korean Dietary Reference Intake (KDRI), and it was less than that of the Korea National Health and Nutrition Examination Surveys (KNHANES). Other nutrient intake levels were similar to the KNHANES. Energy intake proportion of carbohydrates : protein : fat was 56 : 18 : 26, and the ratio of fat intake was higher than that of the KNHANES. Beans, laver, tomato, and yogurt were consumed very frequently. In the results of meal regularity, dietary behaviors and composition of diet were relatively good. It is likely that the patient controlled their diet by eating cautiously and by reducing alcohol drinking and smoking. Our results indicate that FD patients' nutritional status by consumption of nutrients was at a level of normal healthy people and that dietary habits were better than normal adults. However, their fat intake levels were somewhat higher than normal people. Therefore, further research is required to identify the relationship between dietary intakes and FD.

Major Dietary Patterns and Their Associations with Socio-Demographic, Psychological and Physical Factors Among Generally Healthy Korean Middle-Aged Women (건강한 한국 중년 여성에서의 주요 식이패턴과 인구.사회적, 심리적, 신체적 요인 간의 연관성)

  • Lee, Seung-Min;Oh, A-Rim;Ahn, Hong-Seok
    • Korean Journal of Community Nutrition
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    • v.13 no.3
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    • pp.439-452
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    • 2008
  • This study was conducted to identify major dietary patterns of generally healthy Korean middle-aged women and to examine associations of the dietary patterns with socio-demographic, psychological, and physical characteristics. Data were drawn from the 2001 Korean National Health and Nutrition Examination Survey (KNHANES), which is an ongoing national surveillance system. Healthy female subjects aged 40-64 years and provided the health interview examination and food frequency questionnaire (FFQ) data were included. We conducted factor analysis based on daily mean intake frequency of 26 food groups and identified 3 major dietary patterns, healthy, convenient, and alcohol-animal. The healthy pattern was characterized by higher intakes of fish, seafood, poultry, mushroom, seaweed, vegetable, tomato, potato, and fruit, the convenient pattern by processed meat and fish, ramen, noodle and rice cake, sweet snack, egg, bread, and fast food, and the alcohol-animal pattern by alcohol, poultry, meat, and fish. The healthy pattern was significantly related with higher socio-economic status, more stable family structure, healthier mentality, and sounder status of anthropometric and biochemical variables. Those with higher factor scores for the convenience pattern were found to be younger and having higher socio-economic status, higher subjective levels of health, and lower subjective levels of worry on health. Several physical characteristics significantly improved as the levels of the convenience pattern increased. The alcohol-animal pattern was significantly associated with several socio-demographic characteristics including a low education level, a young age, a small family size, and blue-collar jobs. Public health policy makers can utilize the study findings to select prior target populations with higher needs and to tailor dietary behavioral messages to lower chronic disease risks among Korean middle-aged women.