• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2003.10a
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• pp.65-65
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• 2003

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2004.10a
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• pp.97-97
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• 2004

• Proceedings of the Botanical Society of Korea Conference
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• 2000.04a
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• pp.27-27
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• 2000

• The Plant Pathology Journal
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• v.22 no.4
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• pp.353-359
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• 2006

Transgenic Nicotiana benthamiana plants harboring the coat protein(CP) gene of Zucchini green mottle mosaic virus(ZGMMV) were chosen as a model host for the environmental risk assessment of genetically modified plants with virus resistance. This study was focused on whether new virus type may arise during serial inoculation of one point CP mutant of ZGMMV on the transgenic plants. In vitro transcripts derived from the non-functional CP mutant were inoculated onto the virus-tolerant and -susceptible transgenic N. benthamiana plants. Any notable viral symptoms that could arise on the inoculated transgenic host plants were not detected, even though the inoculation experiment was repeated a total of ten times. This result suggests that potential risk associated with the CP-expressiing transgenic plants may not be significant. However, cautions must be taken as it does not guarantee environmental safety of these CP-mediated virus-resistant plants, considering the limited number of the transgenic plants tested in this study. Further study at a larger scale is needed to evaluate the environmental risk that might be associated with the CP-mediated virus resistant plant.

• Korean Journal of Weed Science
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• v.4 no.2
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• pp.115-124
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• 1984

Chlorsulfuron (chemical name : 2-Chloro-N-(4-methoxy-6-methyl-1,3,5-triazin-2-yl)-aminocarbonyl-benzenesulfonamide) is a herbicidally active ingredient which shows effect against susceptible weeds already at such low rates like 5-20g active ingredient per hectare. In the here reported trials metabolism in several sensitive cultivated plants and weeds have been analysed using ^{14}C-labelled active ingredient. The uptake of chlorsulfuron by leaves or the root system is good in all plants species, and translocation takes place either symplasmatically or apoplasmatically. Metabolism takes place in all investigated plant species by development of hydrophile suhstances in roots and shoots. Decomposition of chlorsulfuron in roots and shoots of tolerant species (Triticum aestivum and Hordeum vulgare) to polare substances takes place quantitatively faster and quicker than in susceptible species (Beta vulgaris and Matricaria chamomilla).

• Journal of Radiation Industry
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• v.4 no.3
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• pp.247-252
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• 2010

The present study has been performed to select the osmotic tolerant lines using polyethylene glycol (PEG 6000) through an in vitro and in vivo mutagensis with a gamma-ray. During the screening, we selected three mutant lines that seemed to confer elevated osmotic tolerance in high concentrations of PEG 6000. Fruits of these mutants (Os-HK101, Os-HK102 and Os-HK103) were increased to sugar concentration, L-glutamine acid, vitamin C content and lycopine content than those of the wild type. Also the chlorophyll contents were few decreased more in the three mutant lines than the WT plants. Our results suggest that the Os-HK101 is characterized as osmotic stress tolerance considering the sugar concentration and lycopine content. It is expected that the result of this study can be used for breeding more competitive species with respect to contents in sugar or functional chemicals from the selected osmotic resistant lines.

• Journal of Plant Biology
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• v.17 no.4
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• pp.23-30
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• 1974

The relations of the growth responses of plants, i.e. 4 species of crops, 12 species of roadside trees and 5 species of horticultural plants to cadmium (Cd) to which added soil were studied in pot culture. Growth in dry weight of corn, soybean, barley and wheat plant were decreased with increase of Cd concentration. Damage of corn plant caused by Cd treatment was more or less recovered when it was grown in soil with calcium, but that of other three crops was not recovered when it was with calcium. Although crop plants used here absorbed small amount of Cd through root, Cd content in the shoot was directly proportionate to the concentration of Cd treated to soil. Additions of calcium and sulfur to soil were effectively to change of soil pH, and only of calcium increased exchangeable calcium and cation exchange capacity of its soil. The chlorosis on leaves caused by Cd treatment was observed in 2 species such as Euonymus japonica and Rhododendron yedoense out of 5 species of the horticultural plants, especially at 50ppm of Cd concentration, Euonymus japonica occurred symptoms of chlorosis and difoliation, and at the higher concentration than that the symtoms were sever more and more. At 200 ppm of Cd feeble damage was observed in Pinus koraiensis and Ginkgo biloba but severe chlorosis observed in Robinia pseudoacacia and Sabina chinensis, Buxus koreana, Abies holophylla and Platanus orientalis. Nevertheless those plants had serious damage at 200 ppm of Cd, such symptom was weakened by adding calcium to soil with Cd. There were many Cd tolerant species out of the plants used in thies xperiment, such as Crassula falcata, Chrysanthemum morfolium, Hibiscus syriacus, Ligustrum ovalifolium, Liriodendron tulipeferia, Lespedeza crytobotrys.

• Journal of Environmental Science International
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• v.26 no.10
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• pp.1155-1166
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• 2017

This study selected commonly known species of fruit trees, and re-selected the species that endure the stress of extreme cold weather and physiologically restore themselves to the previous state until the following year. Then we could go ahead to propose the species that were appropriate as urban greening plants in weather condition of any part of the country. To do this, we conducted an experiments for six species of fruit trees based on the preference of the general public and recommendation of the experts; Morus alba (English name: mulberries), Diospyros kaki (English name: Persimmon), Prunus persia (English name: Peach), Elaeagnus umbellata var. coreana (English name: Korean Autumn Olive), Malus domestica 'Alps Otome' (English name: Alps Otome), and Prunus mume (English name: Blue Plum). The experiment verifies whether the trees survive without any stress from the cold weather under the national climate conditions (one in the suburbs of Seoul: Yongin city, one in the central Chungcheong region: Daejeon city, and in the southern Gyeongsang region: Jinju city in Korea). The experiment lasted for a year from August 2016 to August 2017. The levels of electrolytic efflux, chlorophyll content, plant height, fresh weight, and dry weight were measured four times (on August of 2016, January, February, and August of 2017) for each tree planted bare ground outdoors. Results showed that Diospyros kaki, Prunus persia, and Malus domestica 'Alps Otome' were proven durable and resistant to winters of all three areas (one in the suburbs of Seoul: Yongin city, one in the central Chungcheong region: Daejeon city, and in the southern Gyeongsang region: Jinju city in Korea). Especially, the increase of chlorophyll content and the reduction of electrolytic efflux were noticeable in Prunus persia than in the other two species, proving itself as the most cold-tolerant among the six species used in the experiment. In addition, interpreting from the physiological restoration data of one-year span before and after getting through winterer, Prunus persia was proven to be the most cold-tolerant species.

• Korean Journal of Environmental Agriculture
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• v.25 no.3
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• pp.268-275
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• 2006

ALA (5-aminolevulinic acid) has been proposed as a tetrapyrrole-dependent photodynamic herbicide by the action of the protoporphyrinogen IX oxidase (Protox IX). A study was conducted to determine photodynamic herbicidal effect of ALA on seedling growth of rice (Oryza sativa L.) and barnyard grass (Echinochloa crus-galli Beauv. var. oryzicola Ohwi) under dry and wet conditions. ALA effect on early plant growth of rice and barnyardgrass was greatly concentration dependant, suggesting that it promotes plant growth at very low concentration and inhibits at high concentration. No significant difference in herbicidal activity of biologically and synthetically produced ALAs on plant lengths of test plants was observed ALA exhibited significant photodynamic activity regardless of PSDIP and its duration. Significant shoot growth inhibition by ALA soaking treatment exhibited apparently, indicating that ALA absorbed through root system was translocated into shoot part of plants. ALA reduced plant heights of rice and barnyardgrass seedlings by 6% and 27%, respectively, showing more tolerant to ALA in rice under wet condition. Leaf thickness was reduced markedly by ALA with increasing of ALA concentration, due to mainly membrane destruction and severe loss of turgidity in mesophyll cells, although the epidermal was little affected. It was observed that photodynamic herbicidal activity of ALA applied by pre-and post-emergence application exhibited differently on plant species, and that the activity of ALA against susceptible plants was highly correlated with growing condition.

• Journal of The Korean Society of Grassland and Forage Science
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• v.43 no.1
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• pp.42-49
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• 2023

The plant-specific NAC transcription factors control various biological processes, including plant development and stress responses. We have isolated an ANAC032 gene, one of the NAC transcription factor family, which was highly activated by multi-abiotic stresses, including high salt and drought in Arabidopsis. Here, we generated transgenic plants constitutively expressing ANAC032 and its knockout to identify the functional roles of ANAC032 in Arabidopsis under abiotic stress responses. The ANAC032-overexpressing plants showed enhanced tolerance to salinity and drought stresses. The anac032 knockout mutants were observed no significant changes under the high salt and drought conditions. We also monitored the expression of high salt and drought stress-responsive genes in the ANAC032 transgenic plants and anac032 mutant. The ANAC032 overexpression upregulated the expression of stress-responsive genes, RD29A and ERD10, under the stresses. Thus, our data identify that transcription factor ANAC032 plays as an enhancer for salinity and drought tolerance through the upregulation of stress-responsive genes and provides useful genetic traits for generating multi-abiotic stress-tolerant forage crops.