• Title/Summary/Keyword: tolerances

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NITROGEN EXCRETION IN THE BIVALVE MOLLUSCS (이매패의 질소배설 2. 굴)

  • CHIN Pyung;LEE Bok Kyu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.12 no.4
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    • pp.293-296
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    • 1979
  • The effects of temperature and salinity on tile rates of ammonia and amino nitrogen excretion, and oxygen consumption were measured for Crassostrea gigas. There was variability with temperature and salinity changes in both the rates of nitrogen excretion and the proportionality between ,ammonia and amino acids in the excreta, and also in the rates of oxygen consumption. Rates of nitrogen excretion and oxygen consumption were markedly decreased with increase in salinity, especially at high salinity-high temperature, whereas at low salinity-high temperature condition they were significantly increased. These changes are considered as the responses of physiological tolerances to the high temperature stress and the results of the metabolic temperature compensation at the low salinity-high temperature condition. Most of nitrogenous excretory products was ammonia, and large amounts of amino-nitrogen was excreted, and especially the rate of amino-nitrogen excretion was dominant at $32.5\%_{\circ}-22^{\circ}C$. The amounts of amino-nitrogen excreted by animals were decreased in the medium of high salinity and increased in the medium of low salinity through the experimental temperature. The atomic ratios of oxygen consumed to ammonia-nitrogen excreted (O: N ratio) was low at the low temperature $(15^{\circ}C)$, and was high at $22^{\circ}$ and $29^{\circ}C$ in the medium of 32.5 and $37.5\%_{\circ}$ but low in the low salinity $27.5\%_{\circ}$.

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Isolation and Characterization of Wild Yeasts for Improving Liquor Flavor and Quality (주류의 풍미 및 품질 향상을 위한 야생 효모의 분리 및 특성분석)

  • Baek, Seong Yeol;Lee, You Jung;Kim, Jae Hyun;Yeo, Soo-Hwan
    • Microbiology and Biotechnology Letters
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    • v.43 no.1
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    • pp.56-64
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    • 2015
  • It has been known for some time to the wine industry that non-Saccharomyces yeasts play an important role in increasing volatile components through the secretion of extracellular enzymes. The objective of this study was to investigate what types of enzymes are produced by 1,007 non-Saccharomyces yeast strains isolated from Korean fermented foods. Among 1,007 yeast strains, the 566, 45 and 401 strains displayed β-glucosidase, glucanase and protease activity, respectively. In addition, the 563 and 610 strains possessed tolerances against cerulenin and TFL, and the 307 strain was tolerant to 15% ethanol. Yeasts producing harmful biogenic amines and hydrogen sulfide were excluded from further study, and eventually 12 yeast strains belonging to the genera Wickerhamomyces, Hanseniaspora, Pichia, Saccharomyces were identified, based on the 26S rRNA gene sequences. Among the 12 strains, the 9 and 5 strains possessed glucose and ethanol tolerance, respectively. Yeasts belonging to the genus Saccharomyces produced more than 8% alcohol, but non-Saccharomyces yeasts produced only 3% alcohol.

Development and Performance of Cutting and Crushing Instrument of Hair to Prevent Blocking U-trap in Home Drainage System (하수구 막힘 방지를 위한 모발 절단 분쇄 장치 개발 및 성능평가)

  • Kim, Donhue
    • Journal of Korean Society of Environmental Engineers
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    • v.35 no.6
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    • pp.394-399
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    • 2013
  • The recalcitrant hairs which are accumulated in the U-trap equipped to prevent the stench from the drainage produce the phenomenon of blocking the flow of water through the home drainage pathway. The chemical dissolution solutions commercially manufactured are excessively poured into U-trap to solve this blocking problem. In this study, the specially designed instrument was developed for the experiments to cut and crush the hairs of which main component are the recalcitrant keratin proteins. The performance of the instrument for cutting and crushing hairs was investigated using experimental methods. The cutting and weighing method was used in order to obtain the average length of a large amount of hairs cut by instrument. This method is relatively simple to measure the weight of cutting hairs to obtain the average length of the fibers, the values of average length showed not greater tolerances. Also the average cutting number was defined to evaluate the performance of the cutting and crushing instruments designed for this study. We were able to apply these evaluation methods to provide the criteria to obtain the optimal structure of instrument and proper operation time in the given experimental conditions. These experimental methods and results will provide the good example to design and to analyze the various device that can be used for cutting the fibers. The cutting and crushing instrument developed in this study showed the effective abilities to cutting the hairs. The general using the cuttting and crushing instrument at every home would be helpful to prevent water environmental pollution.

A Non-Uniform Convergence Tolerance Scheme for Enhancing the Branch-and-Bound Method (비균일 수렴허용오차 방법을 이용한 분지한계법 개선에 관한 연구)

  • Jung, Sang-Jin;Chen, Xi;Choi, Gyung-Hyun;Choi, Dong-Hoon
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.36 no.4
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    • pp.361-371
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    • 2012
  • In order to improve the efficiency of the branch-and-bound method for mixed-discrete nonlinear programming, a nonuniform convergence tolerance scheme is proposed for the continuous subproblem optimizations. The suggested scheme assigns the convergence tolerances for each continuous subproblem optimization according to the maximum constraint violation obtained from the first iteration of each subproblem optimization in order to reduce the total number of function evaluations needed to reach the discrete optimal solution. The proposed tolerance scheme is integrated with five branching order options. The comparative performance test results using the ten combinations of the five branching orders and two convergence tolerance schemes show that the suggested non-uniform convergence tolerance scheme is obviously superior to the uniform one. The results also show that the branching order option using the minimum clearance difference method performed best among the five branching order options. Therefore, we recommend using the "minimum clearance difference method" for branching and the "non-uniform convergence tolerance scheme" for solving discrete optimization problems.

Persistence and Dislodgeable Residues of Chlorpyrifos and Procymidone in Lettuce Leaves under Greenhouse Condition (상추의 생산단계별 Chlorpyrifos 및 Procymidone의 잔류허용기준 설정)

  • Kim, Young-Sook;Park, Ju-Hwang;Park, Jong-Woo;Lee, Young-Deuk;Lee, Kyu-Seung;Kim, Jang-Eok
    • Korean Journal of Environmental Agriculture
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    • v.21 no.2
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    • pp.149-155
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    • 2002
  • Two pesticides commonly used for lettuce cultivation including chlorpyrifos and procymidone were subjected to a field residue trial to ensure safety of terminal residues in the harvest. After pesticides were applied at standard and double rates in a foliar spray, leaf persistence of their residues was investigated far 10 days prior to harvest. Even though far exceeded the tolerances, initial leaf residues were rapidly dissipated with time and remained only 0.4$\sim$7.2% of the residues in the harvest. As well fitted by the first-order kinetics, biological half-lives of the pesticide residues in lettuce leaves ranged 1.2$\sim$2.6 days. Slow dissipation of the residues in the harvest was observed during storage at room temperature and 4$^{\circ}C$ for 7 days. Portions of dislodgeable residues which resided in detergent washings decreased as time elapsed. Patterns in dissipation and distribution of dislodgeable residues were not largely affected by the application rate of pesticides. It is concluded that timing of pesticide application, that is, pre-harvest interval would be the first factor to determine the terminal residue level in edible portions of lettuce.

Screening of saline tolerant plants and development of biological monitoring technique for saline stress . 1. Survey of vegetation in saline region and determination of saline tolerance of the plant species of the region. (내염성 식물의 탐색 및 생물학적 염해 모니터링 기술의 개발 1. 염해지 식생분석 및 식물종의 내염성 평가)

  • Kang, Byeung-Hoa;Shim, Sang-In
    • Korean Journal of Environmental Agriculture
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    • v.17 no.1
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    • pp.26-33
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    • 1998
  • This experiment was conducted to classify the plant species occurring in the saline reclaimed land by saline tolerance. The vegetation of reclaimed land was composed of various plant species from halophyte to glycophyte showing different saline tolerances. In the investigated saline region, reclaimed land of Youngjong island, Inchun city, 175 species belonging to 32 families were found. Our survey was carried out in two region, having different salinity due to different desalinization. The electricalconductivity (EC) of more saline region showed 48.0mS/cm nd the other region showed 13.0mS/cm. It is assumed that intensity of precipitation and runoff of rainfall may cause salinity gradient in the investigated region. The plant species occurred in the experimental region were classified as 72 species of annual, 42 species of biennial, and 61 species of perennial according to life cycle. For knowing relationship between vegetation of saline region and saline tolerance of occurring species, we tested the saline susceptibility of plant species collected at the saline regions. Testing plants were cultured by nutrient solution containing 200 mM NaCl, the critical concentration of survival in glycophytes. The saline tolerance was graded by the growing capacity in the sand-culture system. The more saline-tolerant species screened by sand culture were Atriplex gmelini, Suaeda asparagoides, Aster tripolium, Suaeda maritima, Salicornia herbacea, and Suaeda japonica. The most saline tolerant family was Chenopodiaceae. Poaceae, Cyperaceae, and Brassicaceae showed relatively high tolerance to saline stress. In the course of growth under the high saline condition, the most noticeable change was the darkening of leaves by increasing of chlorophyll content. The chlorophyll contents were increased with saline stress in most species.

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Solvent-tolerant Lipases and Their Potential Uses (유기용매 내성 리파아제와 그 이용가능성)

  • Joo, Woo Hong
    • Journal of Life Science
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    • v.27 no.11
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    • pp.1381-1392
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    • 2017
  • This review described solvent-tolerant lipases and their potential industrial, biotechnological and environmental impacts. Although organic solvent-tolerant lipase was first reported in organic solvent-tolerant bacterium, many organic solvent-tolerant lipases are in not only solvent-tolerant bacteria but also solvent-intolerant bacterial and fungal strains, such as the well-known Bacillus, Pseudomonas, Streptomyces and Aspergillus strains. As these lipases are not easily inactivated in organic solvents, there is no need to immobilize them in order to prevent an enzyme inactivation by solvents. Therefore, the solvent-tolerant lipases have the potential to be used in many biotechnological and biotransformation processes. With the solvent-tolerant lipases, a large number insoluble substrates become soluble, various chemical reactions that are initially impossible in water systems become practical, synthesis reactions (instead of hydrolysis) are possible, side reactions caused by water are suppressed, and the possibility of chemoselective, regioselective and enantioselective transformations in solvent and non-aqueous systems is increased. Furthermore, the recovery and reuse of enzymes is possible without immobilization, and the stabilities of the lipases improve in solvent and non-aqueous systems. Therefore, lipases with organic-solvent tolerances have attracted much attention in regards to applying them as biocatalysts to biotransformation processes using solvent and non-aqueous systems.

Acid Tolerance Response of Streptococcus mutans at Anaerobic Condition (Streptococcus mutans의 혐기적 산 내성도 평가)

  • Han, Yang-Keum;Song, Sang-Sun;Lee, In-Soo
    • Journal of dental hygiene science
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    • v.1 no.1
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    • pp.7-11
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    • 2001
  • Streptococcus mutans is one of the primary bacteria that cause dental caries which further result in plaque build up. Acid production resulted from carbohydrate metabolism can threaten survival of the bacteria. However some populations of S. mutans which are exposed to low acidic condition for a period of time would develop resistance and tolerance of cells to acidity that will enhance the chance of survival. Similar acid tolerances has been reported in case of Salmonella enterica serovar typhimurium, E. coli, Shigella flexneri. These acid tolerance responses(ATR) have been evolved in a similar manner as S. mutans. The protein produced in acidic condition has been proven to be important for ATR and confirmed by using chloramphenicol procedure. We hypothesize here that proteins synthesized in response to acid shock and other elements are important for ATR of cells. In this study we have confirmed that S. mutans developed acid tolerance and resistance against anaerobic condition. Mutational DNA analysis responsible for acid tolerance should be additionally required in the future. Since the development of acid tolerance that is essential for the survival of S. mutans and development of dental caries, ATR of S. mutans shoule be farther to prevent dental caries.

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Optical Design of the Integrated Triplexer Fabricated by Micro Block Stacking Method (MBS 방법으로 제작한 집적형 Triplexer의 광학 설계)

  • Yoon, Hyun-Jae;Kim, Jong-Hyuk
    • Korean Journal of Optics and Photonics
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    • v.22 no.4
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    • pp.191-197
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    • 2011
  • In this paper, we have designed an integrated triplexer which is the basic component for a FTTH(Fiber To The Home) system which can transmit CATV and voice/data at the same time in a single fiber. The integrated triplexer can be fabricated with a novel technique of "Micro-Block Stacking (MBS)" method which automatically aligns the optical components in the optical beam pass using accurate ceramic holders. We analyze the displacement of the optical focus according to the tolerances of the component dimensions and the assembly process using code V simulator. For the transmitter, the most serious shift of the focal points is caused by the displacements of the LD spot. So the focal point moves up to $72{\mu}m$ from the center point for ${\pm}25{\mu}m$, ${\pm}25{\mu}m$, ${\pm}30{\mu}m$ displacements. For the receiver the most serious shift of the focal points is caused by the displacements of a 0.8mm ball lens (for the analog receiving part) and a micro ball lens (for the digital receiving part), and the focal point moves up to $55{\mu}$ for ${\pm}55{\mu}m$, ${\pm}5{\mu}m$, ${\pm}55{\mu}m$ micro ball lens displacements.

IGRINS Mirror Mount Design for Three Off-Axis Collimators and One Slit-Viewer Fold Mirror

  • Rukdee, Surangkhana;Park, Chan;Kim, Kang-Min;Lee, Sung-Ho;Chun, Moo-Young;Yuk, In-Soo;Oh, Hee-Young;Jung, Hwa-Kyoung;Lee, Chung-Uk;Lee, Han-Shin;Rafal, Marc D.;Barnes, Stuart;Jaffe, Daniel T.
    • Journal of Astronomy and Space Sciences
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    • v.29 no.2
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    • pp.233-244
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    • 2012
  • The Korea Astronomy and Space Science Institute and the Department of Astronomy at the University of Texas at Austin are developing a near infrared wide-band high resolution spectrograph, immersion grating infrared spectrometer (IGRINS). The compact white-pupil design of the instrument optics uses seven cryogenic mirrors, including three aspherical off-axis collimators and four flat fold mirrors. In this study, we introduce the optomechanical mount designs of three off-axis collimating mirrors and one flat slit-viewer fold mirror. Two of the off-axis collimators are serving as H and K-band pupil transfer mirrors, and are designed as system alignment compensators in combination with the H2RG focal plane array detectors in each channel. For this reason, the mount designs include tip-tilt and parallel translation adjustment mechanisms to properly perform the precision alignment function. This means that the off-axis mirrors' optomechanical mount designs are among the most sensitive tasks in all IGRINS system hardware. The other flat fold mirror is designed within its very limitedly allowed work space. This slit-viewer fold mirror is mounted with its own version of the six-point kinematic optics mount. The design work consists of a computer-aided 3D modeling and finite element analysis (FEA) technique to optimize the structural stability and the thermal behavior of the mount models. From the structural and thermal FEA studies, we conclude that the four IGRINS mirror mounts are well designed to meet all optical stability tolerances and system thermal requirements.