• Title/Summary/Keyword: tolerance or resistance

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Endophytic fungi harbored in Chinese native gramineous plants

  • Wang, Zhiwei;Ji, Yanling
    • 한국균학회소식:학술대회논문집
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    • 2009.10a
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    • pp.44-56
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    • 2009
  • $Epichlo\ddot{e}$ endophytes, including Neotyphodium spp. and $Epichlo\ddot{e}$ spp., enhance plant growth, mediate more plant tolerance or resistance to biotic and abiotic stresses, and also synthesis various biologically active compounds in their host plants, and important in many areas. In early stages, most of $epichlo\ddot{e}$ endophytes were described during surveys practiced by American, European and Oceania scientists, while fungal endophytes within native Asian plants were poorly investigated. In recent years, an $Epichlo\ddot{e}$ sp. and 4 Neotyphodium spp. were described in cool season Chinese native gramineous plants. Most of Chinese native Neotyphodium spp. were presumed as hybrids originated from members of ETC and EBY. Investigation on NRPS genes shows lack of toxic ergopeptines and potential production of peramine. Biological and ecological roles of Chinese native $epichlo\ddot{e}$ endophytes should be investigated in future, and it will be very valuable if we can have some joint projects with Korean scientists for Asian native $epichlo\ddot{e}$ endophytes.

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Study on the Compositions of Photosensitive Resistor Paste Using Epoxy Acrylate Oligomers and Conductive Carbonblack (에폭시 아크릴레이트 올리고머와 전도성 카본블랙을 이용한 감광성 저항 페이스트 조성 연구)

  • Park, Seong-Dae;Kang, Nam-Kee;Lim, Jin-Kyu;Kim, Dong-Kook
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2008.11a
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    • pp.421-421
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    • 2008
  • Generally, the polymer thick-film resistors for embedded organic or hybrid substrate are patterned by screen printing so that the accuracy of resistor pattern is not good and the tolerance of resistance is too high(${\pm}$20~30%). To reform these demerits, a method using Fodel$^{(R)}$ technology, which is the patterning method using a photosensitive resin to be developable by aqueous alkali-solution as a base polymer for thick-film pastes, was recently incorporated for the patterning of thermosetting thick-film resistor paste. Alkali-solution developable photosensitive resin system has a merit that the precise patterns can be obtained by UV exposure and aqueous development, so the essential point is to get the composition similar to PSR(photo solder resist) used for PCB process. In present research, we made the photopatternable resistor pastes using 8 kinds of epoxy acrylates and a conductive carbonblack (CDX-7055 Ultra), evaluated their developing performance, and then measured the resistance after final curing. To become developable by alkali-solution, epoxy acrylate oligomers with carboxyl group were prepared. Test coupons were fabricated by patterning copper foil on FR-4 CCL board, plating Ni/Au on the patterned copper electrode, applying the resistor paste on the board, exposing the applied paste to UV through Cr mask with resistor patterns, developing the exposed paste with aqueous alkali-solution (1wt% $Na_2CO_3$), drying the patterned paste at $80^{\circ}C$ oven, and then curing it at $200^{\circ}C$ during 1 hour. As a result, some test compositions couldn't be developed according to the kind of oligomer and, in the developed compositions, the measured resistance showed different results depending on the paste compositions though they had the same amount of carbonblack.

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Greenhouse Evaluation of Melon Rootstock Resistance to Monosporascus Root Rot and Vine Decline as Well as of Yield and Fruit Quality in Grafted 'Inodorus' Melons

  • Jang, Yoonah;Huh, Yun-Chan;Park, Dong-Kum;Mun, Boheum;Lee, Sanggyu;Um, Yeongcheol
    • Horticultural Science & Technology
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    • v.32 no.5
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    • pp.614-622
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    • 2014
  • Melons (Cucumis melo L.) are generally grafted onto Cucurbita rootstocks to manage soilborne pathogens such as Monosporascus root rot and v ine decline (MRR/VD) and Fusarium wilt. However, g rafting onto Cucurbita rootstocks reportedly results in the reduction of fruit quality. In this study, the resistance to MRR/VD, yield, and fruit quality of melons grafted onto melon rootstocks were evaluated under greenhouse conditions. Eight melon rootstocks (R1 to R8) were used and the inodorus melon 'Homerunstar' was used as scion. Melon rootstocks R1 to R6 were selected based on resistance to MRR/VD under greenhouse conditions. Non-grafted 'Homerunstar' and plants grafted onto squash interspecific hybrid 'Shintozwa' rootstock (Cucurbita maxima D. ${\times}$ C. moschata D.) served as controls. Grafted melons were cultivated in the greenhouse infested with Monosporascus cannonballus during two growing seasons (summer and autumn). The responses to MRR/VD, yield, and fruit quality differed depending on the rootstocks and growing season. The melons grafted onto 'Shintozwa' exhibited less severe disease symptoms and higher survival rates than non-grafted melons in both seasons. While the melon rootstocks in the summer cultivation did not increase the survival rate compared to non-grafted melons, the melon rootstocks R1 and R2 in the autumn cultivation led to higher survival rates. The melon rootstocks resistant to MRR/VD increased the percentage of marketable fruits and marketable yields. Grafting onto the melon rootstocks caused little or no reduction of fruit quality such as low calcium content, fruit softening, and vitrescence, especially in lower-temperature autumn season. Accordingly, these results suggest that grafting onto the melon rootstocks may increase the tolerance to MRR/VD and the marketable yield without a reduction of fruit quality.

A Safety Evaluation of Genetically Modified Feedstuffs for Livestock Production; the Fate of Transgenic DNA and Proteins

  • Beever, D.E.;Glenn, K.;Phipps, R.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.16 no.5
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    • pp.764-772
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    • 2003
  • Two genetic constructs used to confer improved agronomic characteristics, namely herbicide tolerance (HT) in maize and soyabean and insect resistance (Bt) in maize, are considered in respect of feeding to farm livestock, animal performance and the nutritional value and safety of animal products. A review of nucleic acid (DNA) and protein digestion in farm livestock concludes that the frequency of intact transgenic DNA and proteins of GM and non-GM crops being absorbed is minimal/non existent, although there is some evidence of the presence of short fragments of rubisco DNA of non-GM soya in animal tissues. It has been established that feed processing (especially heat) prior to feeding causes significant disruption of plant DNA. Studies with ruminant and non-ruminant farm livestock offered GM feeds demonstrated that animal performance and product composition are unaffected and that there is no evidence of transgenic DNA or proteins of current GM in the products of animals consuming such feeds. On this evidence, current HT and Bt constructs represent no threat to the health of animals, or humans consuming the products of such animals. However as new GM constructs become available it will be necessary to subject these to rigorous evaluation.

Evaluation of the Autonomic Nervous System Using Laser Doppler (Laser Doppler를 이용한 자율신경 기능 평가 방법)

  • Nam, Sang-Hee;Shin, Jung-Woog;Cho, Jong-Man
    • Proceedings of the KOSOMBE Conference
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    • v.1993 no.11
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    • pp.51-54
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    • 1993
  • The purpose of this research is to investigate the relationship between the blood flow and autonomic nervous system which regulates the circulation system. After the oral glucose tolerance test(GTT) the velocity changes of the blood flow were measured from the normal, hyperglycemia and diabetic neuropathy using the laser doppler. The FFT analyses showed that the spectrums were concentrated around the particular bands(0.02-0.166Hz, 0.8-1.1Hz) for the normal, while almost uniform distribution of the spectrums was observed for the abnormal. The blood glucose affects the compliance of the blood vessels and viscosity and finally causes the resistance problem in the blood vessels. An index PG was introduced, which was determined by the ratio of the change or the integral power spectrum in the range between 0.02 and 0.166Hz to change of the blood glucose before and after GTT. The index shows 1.0. 0.3 and 0.1 for the normal, hyperglycemia and diabetic neuropathy, respectively. As a conclusion, this spectrum analysis shows the potential of checking the progress or the diabetic, also car be used to evaluate the vasomotion which are regulated by the autonomic nervous system.

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'Arirang', Early-flowering Pure White Gladiolus with Purple Core (조생 순백색 자주 화심 글라디올러스 '아리랑')

  • Cho, Hae Ryong;Rhee, Hye Kyung;Lim, Jin Hee
    • FLOWER RESEARCH JOURNAL
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    • v.18 no.2
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    • pp.142-146
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    • 2010
  • 'Arirang', an early-flowering pure white gladiolus with purple core, was released by the National Institute of Horticultural & Herbal Science (NIHHS) in 1998. The selection was derived from the cross between the progenies of the early flowering dark orange 'Arianne' and bright red domestic cultivars 'Hongkwang'. The breeding was conducted in 1993 and finally selected in 1998 after five (5) years (1994 to 1998) of experimentation. 'Arirang' is a cut flower with bright white color petals and purple core which exhibited early flowering and vigor without stem bending. These can be cultivated in areas or regions with strong winds during summer. 'Arirang' has a very firm growth form and sturdy branches which do not bend when planted in the open fields. Likewise, the plant had high tolerance to flooding and resistance hence could grow vigorously in open areas. It is a fast- growing plant which exhibits high corm production rate. 'Arirang' had a 10.5 cm wide flower and 140 cm plant height. During summer, the average number of days to flowering was recorded at 75 days of 'Arirang'. Top dressing must be conducted once or twice during corm production to enhance corm quality and quantity.

A Study of the usage of Corrugated Fiberboard Cartons for Domestic Fresh Produce and Their Physical Properties: Focused on a Gyeongsan City, North Gyeongsan Province (국내 신선 농산물 포장용 골판지 상자의 이용 실태 및 물리적 특성 연구: 경북 경산 소재 지역 중심으로)

  • Kim, Minhwi;Lee, Myungho;Boonsiriwit, Athip;Lee, Youn Suk
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.28 no.1
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    • pp.55-66
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    • 2022
  • In this study, forty corrugated fiberboard (CF) boxes for fresh produce were collected from farms located in Gyeongsan city and evaluated its characterizations and physical properties according to Korean Standard (KS). The basis weight, flute, and paperboard combination were determined for the characterizations of CF boxes. The measured basis weights of all samples were within the tolerance limit referred to KS and the flutes were B/F, EB/F, and AB/F flute, respectively. The bursting strength (BS), edgewise crush resistance (ECR), and compression strength (CS) were tested to evaluate the physical properties. There are four single-walled (SW) CF boxes showed BS and ECR higher than 638 kPa and 3.30 kN/m, respectively. One SW CF boxes showed BS and ECR higher than 785 kPa and 3.43 kN/m, respectively. In case of double-walled (DW) CF boxes, sixteen CF boxes showed BS and ECR higher than 785 kPa, 4.96 kN/m (AB flute), or 4.90 (EB flute), respectively. The other nineteen CF boxes showed BS and ECR higher than 981 kPa, 5.29 kN/m (AB flute), or 5.20 (EB flute), respectively. All the CF boxes except two samples showed the acceptable compression strength in reference to the guideline of KS.

Characterization of Lactobacillus acidophilus Isolated from Piglets and Chicken

  • Ahn, Y.T.;Lim, K.L.;Ryu, J.C.;Kang, D.K.;Ham, J.S.;Jang, Y.H.;Kim, H.U.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.12
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    • pp.1790-1797
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    • 2002
  • Lactic acid bacteria were isolated from piglets and chicken and characterized. Lactic acid bacteria showing resistance to low pH and bile, adhesion to intestinal epithelium cells, and the inhibition of Escherichia coli and Salmonella spp. were identified as Lactobacillus acidophilus. L. acidophilus PF01 survived for 2 h in MRS broth adjusted to pH 2. L. acidophilus CF07 was less resistant than L. acidophilus PF01 to pH 2, but survived at pH 2.5 for 2 h. Both of isolates were able to grow in MRS broth containing 0.3% (w/v) bile, with L. acidophilus CF07 being more tolerant to bile than L. acidophilus PF01. L. acidophilus PF01 and CF07 adhered specifically to the duodenal and jejunal epithelium cells of piglet, and the cecal and duodenal epithelium cells of chicken, respectively. Both of isolates did not adhere to the epithelium cells of the various animal intestines from which they were isolated. When L. acidophilus was cultured with E. coli and Salmonella spp. in MRS broth, MRS broth containing 2% skim milk powder or modified tryptic soy broth at $37^{\circ}C$, L. acidophilus PF01 and CF07 inhibited the growths of E. coli K88 and K99, and S. enteritidis and S. typhimurium, respectively. Both of isolates were found to possess the essential characteristics of probiotic lactic acid bacteria for piglet and chicken.

Creating damage tolerant intersections in composite structures using tufting and 3D woven connectors

  • Clegg, Harry M.;Dell'Anno, Giuseppe;Partridge, Ivana K.
    • Advances in aircraft and spacecraft science
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    • v.6 no.2
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    • pp.145-156
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    • 2019
  • As the industrial desire for a step change in productivity within the manufacture of composite structures increases, so does the interest in Through-Thickness Reinforcement technologies. As manufacturers look to increase the production rate, whilst reducing cost, Through-Thickness Reinforcement technologies represent valid methods to reinforce structural joints, as well as providing a potential alternative to mechanical fastening and bolting. The use of tufting promises to resolve the typically low delamination resistance, which is necessary when it comes to creating intersections within complex composite structures. Emerging methods include the use of 3D woven connectors, and orthogonally intersecting fibre packs, with the components secured by the selective insertion of microfasteners in the form of tufts. Intersections of this type are prevalent in aeronautical applications, as a typical connection to be found in aircraft wing structures, and their intersections with the composite skin and other structural elements. The common practice is to create back-to-back composite "L's", or to utilise a machined metallic connector, mechanically fastened to the remainder of the structure. 3D woven connectors and selective Through-Thickness Reinforcement promise to increase the ultimate load that the structure can bear, whilst reducing manufacturing complexity, increasing the load carrying capability and facilitating the automated production of parts of the composite structure. This paper provides an overview of the currently available methods for creating intersections within composite structures and compares them to alternatives involving the use of 3D woven connectors, and the application of selective Through-Thickness Reinforcement for enhanced damage tolerance. The use of tufts is investigated, and their effect on the load carrying ability of the structure is examined. The results of mechanical tests are presented for each of the methods described, and their failure characteristics examined.

Overexpression of ginseng cytochrome P450 CYP736A12 alters plant growth and confers phenylurea herbicide tolerance in Arabidopsis

  • Khanom, Sanjida;Jang, Jinhoon;Lee, Ok Ran
    • Journal of Ginseng Research
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    • v.43 no.4
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    • pp.645-653
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    • 2019
  • Background: Cytochrome P450 enzymes catalyze a wide range of reactions in plant metabolism. Besides their physiological functions on primary and secondary metabolites, P450s are also involved in herbicide detoxification via hydroxylation or dealkylation. Ginseng as a perennial plant offers more sustainable solutions to herbicide resistance. Methods: Tissue-specific gene expression and differentially modulated transcripts were monitored by quantitative real-time polymerase chain reaction. As a tool to evaluate the function of PgCYP736A12, the 35S promoter was used to overexpress the gene in Arabidopsis. Protein localization was visualized using confocal microscopy by tagging the fluorescent protein. Tolerance to herbicides was analyzed by growing seeds and seedlings on Murashige and Skoog medium containing chlorotoluron. Results: The expression of PgCYP736A12 was three-fold more in leaves compared with other tissues from two-year-old ginseng plants. Transcript levels were similarly upregulated by treatment with abscisic acid, hydrogen peroxide, and NaCl, the highest being with salicylic acid. Jasmonic acid treatment did not alter the mRNA levels of PgCYP736A12. Transgenic lines displayed slightly reduced plant height and were able to tolerate the herbicide chlorotoluron. Reduced stem elongation might be correlated with increased expression of genes involved in bioconversion of gibberellin to inactive forms. PgCYP736A12 protein localized to the cytoplasm and nucleus. Conclusion: PgCYP736A12 does not respond to the well-known secondary metabolite elicitor jasmonic acid, which suggests that it may not function in ginsenoside biosynthesis. Heterologous overexpression of PgCYP736A12 reveals that this gene is actually involved in herbicide metabolism.