• Journal of Plant Biotechnology
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• v.47 no.4
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• pp.324-329
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• 2020

Reed (Phragmites spp.) is a rhizomatous plant of the Poaceae family and is known as high tolerant plant to heavy metal contaminants. This plant is widely recognized as a Cd root-accumulator, but improved heavy metal tolerance and uptake capacity are still required for phytoremediation efficiency. To enhance capacity of hyperaccumulator plants, ethyl methane sulfonate (EMS) as chemical mutagen has been introduced and applied to remediation approaches. This study aimed to select EMS-mutagenized reeds representing high Cd resistance and large biomass and to investigate their ability of Cd accumulation. After 6 months cultivation of M₂ mutant reeds under Cd stress conditions (up to 1,500 µM), we discovered seven mutant individuals that showed good performances like survivorship, vitality, and high accumulation of Cd, particularly in their roots. Compared to wild type (WT) reeds as control, on average, dry weight of mutant type (MT) reeds was larger by 2 and 1.5 times in roots and shoots, respectively. In addition, these mutant plants accumulated 6 times more Cd, mostly in the roots. In particular, MT8 reeds showed the greatest ability to accumulate Cd. These results suggest that EMS mutagenesis could generate hyperaccumulator plants with enhanced Cd tolerance and biomass, thereby contributing to improvement of phytoremediation efficiency in Cd-contaminated soil or wastewater. Further studies should focus on identifying Cd tolerance mechanisms of such EMS-mutagenized plants, developing techniques for its biomass production, and investigating the practical potential of the EMS mutants for phytoremediation.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.58 no.3
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• pp.578-585
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• 2009

In this paper, we present the design of an electromagnetic scanning mirror with torsional springs. The scanning mirror consisting of torsional springs and electromagnetic coils was designed for the applications of laser animation systems. We analyzed and optimized three types of torsional springs, namely, straight beam springs (SBS), classic serpentine springs (CSS), and rotated serpentine springs (RSS). The torsional springs were analyzed in terms of electrical resistance, fabrication error tolerance, and resonance mode separation of each type using analytical formula or numerical analysis. The RSS has advantages over the others as follows: 1) A low resistance of conductors, 2) wide resonance mode separation, 3) strong fabrication error tolerance, 4) a small footprint. The double-layer coils were chosen instead of single-layer coils with respect to electromagnetic forces. It resulted in lower power consumption. The geometry of the scanning mirror was optimized by calculations; RSS turn was 12 and the width of double-layer coil was $100{\mu}m$, respectively. When the static rotational angle is 5 degrees, the power consumption of the mirror plate was calculated to be 9.35 mW since the resistance of the coil part and a current is $122{\Omega}$ and 8.75 mA, respectively. The power consumption of full device including the mirror plate and torsional springs was calculated to be 9.63 mW.

• Journal of Microbiology and Biotechnology
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• v.12 no.4
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• pp.576-582
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• 2002

Probiotics are live microbial feed supplements which beneficially affect the host animal by improving its intestinal microflora. To select the best Lactobacillus spp. as a chicken probiotic, probiotic characteristics of 10 selected Lactobacillus strains isolated from chicken cecum or obtained from KCTC were investigated. The strains were examined for resistance to pH 2.0 and 0.3% oxgall, and adhesion to cecal mucus and cecal epithelial cells. All strains grew in MRS containing 0.3% oxgall. However, Lb. plantarum AYM-10, Lb. fermentum YL-3, AYM-3, and Lb. paracasei YL-6 showed relatively high resistance to 0.3% oxgall. Lb. fermentum YL-3, YM-5, AYM-3, and Lb. paracasei YL-6 survived 4 hours of incubation at pH 2.0. Lb. fermentum YL-3, KCTC 3112, and Lb. plantarum AYL-5 were strongly adhesive to cecal mucus, while the rest showed moderate or low adhesion. Lb. plantarum AYM-10, AYL-1, and AYL-5 had good adhering properties to cecal epithelial cells (30.7$\pm$10.82, 40.2$\pm$20.90, and 14.5$\pm$4.22, respectively). Lb. fermentum YL-3, AYM-3, and KCTC 3547 showed Intermediate adhesion ability, and Lb. plantarum showed better adhesion ability to cecal epithelial cells than Lb. fermentum. Attached Lb. fermentum YL-3 to cecum after 60 min incubation was confirmed using CLSM. Lb. fermentum YL-3 attached to a matrix which was composed of a mucus layer adjacent to intracrypts and pericryptal region. Some Lb. fermentum YL-3 bound to mucosal epithelial cells. From these results, Lb. fermentum YL-3 was selected as a chicken probiotic. In vivo trials of chicks inoculated with Lb. fermentum YL-3 had decreased Salmonella population in cecal contents and livers (p<0.5).

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2002.05a
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• pp.852-855
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• 2002

In order to investigate the fracture behaviors(penetration modes) and resistance to penetration during ballistic impact of surface hardening treated Aluminum, Titanium alloy laminates, ballistic tests were conducted. In this paper, Anodized Al 5083-H131 alloy laminates and nitrified Ti (Gr.2) alloy laminates were used to achieve higher surface hardness. Surface hardness test were conducted using a Micro victor's hardness tester and thickness of surface hardening treated specimens was measured by video microscope. Resistance to penetration is determined by the protection ballistic limit($V_50$), a statistical velocity with 50% probability far complete penetration. Fracture behaviors and ballistic tolerance, described by penetration modes, are respectfully observed at and above ballistic limit velocities, as a result of $V_50$ test and Projectile Through Plates (PTP) test methods. PTP tests were conducted with $0^{\circ}$ obliquity at room temperature using 5.56mm ball projectile. $V_50$ tests with $0^{\circ}$ obliquity at room temperature were conducted with projectiles that were able to achieve near or complete penetration during PTP tests.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2000.11a
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• pp.507-512
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• 2000

The ob.jective of this study is to determine fracture behaviors(penetrati0n modes) and resistance to penetration duringballistic impact of Al 5052-H34 alloy laminates and anodized Al 5052-H34 alloy laminates. Resistance to penetration is determined by $V_{50}$ ballistic limit, a statical velocity with 50% probability for complete penetration, test method. Fracture behaviors and ballistic tolerance, described by penetration modes, are respectfully observed that result from V50 test and Projectile Through Plates (PTP) test at velocities greater than $V_{50}$. PTP tests were conducted with 0" obliquity at room temperature using 5.56mm ball projectile. $V_{50}$ tests with 0" obliquity at room temperature were conducted with projectiles that were able to achieve near or complete penetration during PTP tests. Surface Hardness, resistance to penetration, and penetration modes of A1 5052-H34 alloy laminates compared to those of anodized Al 5052-H34 alloy laminates.y laminates.

• Journal of the Korean Society for Precision Engineering
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• v.20 no.3
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• pp.178-186
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• 2003

In order to investigate the fracture behaviors (penetration modes) and the resistance to penetration during ballistic impact of Al 5052-H34 alloy laminates, cold-rolled Al 5052-H34 alloy laminates, anodized Al 5052-H34 alloy laminates, and anodized Al 5052-H34 alloy after cold-rolling, a ballistic testing was conducted. In general, superior armor materials are brittle materials which have a high hardness. Ballistic resistance of these materials was measured by a protection ballistic limit (V$_{50}$), a statistical velocity with 50% probability fur incompletete penetration. Fracture begaviors and ballistic tolerance, described by penetration modes, ate observed from the results from the results of V$_{50}$ test and Projectile Through Plates (PTP) test at velocities greater than V$_{50}$, respectively. PTP tests were conducted with 0$_{\circ}$obliquity at room temperature using 5.56mm ball projectile. V$_{50}$ tests with 0$_{\circ}$obliquity at room temperature were concucted with projectiles that could achieve neat or complete penetration during PTP tests. Surface hardness, resistance to penetration, and penetration modes of Al 5052-H34 alloy laminates are compared to those of cold-rolled Al 5052-H34 alloy laminates and anodized Al 5052-H34 alloy laminates and anodized Al 5052-H34 cold-rolled alloy.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1997.10a
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• pp.820-824
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• 1997

In order to investigate the effect of surface treatment(Anodizing) and rolling in Al 5083-H131 alloy, ballistic testing was conducted. Ballistic resistance of these materials. was measured by protection ballistic limit(V$_{50}$), a statical velocity with 50% probability for incomplete penetration. Fracture behaviors and ballistic tolerance, described by penetration modes, are observed respectfully, resulting from V$_{50}$ test and Projectile Through Plates(PTP) test at velocities greater than V$_{50}$. PTP tests were conducted with 0$^{\circ}$obliquity at room temperature were also conducted with projectiles that were able to achieve near or complete penetration during PTP tests. Resistance to penetration, and penetration modes of Al 5052-H34 alloy, compared to those of Al 5083-H131 alloy.alloy.

• Plant Biotechnology Reports
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• v.5 no.4
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• pp.323-329
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• 2011

MuSI, a gene that corresponds to a domain that contains the rubber elongation factor (REF), is highly homologous to many stress-related proteins in plants. Since MuSI is up-regulated in the roots of plants treated with cadmium or copper, the involvement of MuSI in cadmium tolerance was investigated in this study. Escherichia coli cells overexpressing MuSI were more resistant to Cd than wild-type cells transfected with vector alone. MuSI transgenic plants were also more resistant to Cd. MuSI transgenic tobacco plants absorbed less Cd than wild-type plants. Cd translocation from roots to shoots was reduced in the transgenic plants, thereby avoiding Cd toxicity. The number of short trichomes in the leaves of wild-type tobacco plants was increased by Cd treatment, while this was unchanged in MuSI transgenic tobacco. These results suggest that MuSI transgenic tobacco plants have enhanced tolerance to Cd via reduced Cd uptake and/or increased Cd immobilization in the roots, resulting in less Cd translocation to the shoots.

• Journal of Yeungnam Medical Science
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• v.12 no.2
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• pp.319-330
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• 1995

The influence of normal late pregnancy on insulin action and insulin secretion was studied in the Sprague-Dawley female rats. On 20th day after mating, intravenous glucose tolerance test(IVGTT) was performed in non pregnant control and pregnant rats. As results of IVGTT, glucose disappearance rate was not significantly different in both groups, but secretory response of insulin was significantly(p<0.05) increased in pregnant rat. And the ratio of insulin/glucose was significantly higher in pregnant rats, which means existence of insulin resistance. These insulin resistance was overcomed by increased secretory response of pancreatic insulin. Insulinogenic index(${\Delta}$ insulin/glucose - 5 min) was highly significantly (r=0.62, p<0.01) correlated with progesterone concentration. Glycogen level and amounts of $^{14}C$-glucose incorporated into glycogen after IVGTT were significantly(p<0.05) decreased in the liver, but were not changed significantly in soleus. Glycogen synthase activity of soleus and liver was not differ significantly in the both groups. Insulin binding at varying concentrations of insulin to crude membrane of pregnant liver was not significantly different from control. In conclusions, although these pregnant rats were normal glucose tolerance due to increased secretory response of insulin, that was correlated with progesterone concentration, pregnant rat had insulin resistance. The mechanisms of insulin resistance were not related to defect of insulin binding phase and glycogen synthase, but suggest pre-receptor and/or postreceptor phase.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.66 no.1
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• pp.8-17
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• 2021

As rice originates from tropical regions, low temperature stress during the germination stage in temperate regions leads to serious problems inhibiting germination and seedling establishment. Identifying and characterizing quantitative trait loci (QTLs) for low-temperature germination (LTG) resistance help accelerate the development of rice cultivars with LTG tolerance. In this study, we identified QTLs for LTG tolerance (qLTG5, qLTG9) and germination coefficient of velocity under optimal conditions (OGCV) (qOGCV7, qOGCV9) using 129 recombinant inbred lines (RILs) derived from the cross between a low-temperature sensitive line Milyang23 and a low-temperature tolerant variety Gihobyeo. qLTG9 and qOGCV9 were detected at the same location on chromosome 9. At both LTG QTLs (qLTG5 and qLTG9), the alleles for LTG tolerance were contributed by the japonica variety Gihobyeo. At qOGCV7 and qOGCV9, the alleles for low temperature tolerance were derived from Milyang23 and Gihobyeo, respectively. The RILs with desirable alleles at two or more QTLs, i.e., GroupVII: qLTG5+qLTG9 (qOGCV9) and GroupVIII: qLTG5+qOGCV7+qLTG9 (qOGCV9), showed stable tolerance under low-temperature stress. Our results are expected to contribute to the improvement of tolerance to low-temperature and anaerobic stress in japonica rice, which would lead to the wide adoption of direct-seeding practices.