• BMB Reports
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• v.37 no.5
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• pp.538-545
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• 2004

A new CRT binding factor (CBF) gene designated Cbcbf25 was cloned from Capsella bursa-pastoris, a wild grass, by the rapid amplification of cDNA ends (RACE). The full-length cDNA of Cbcbf25 was 898 bp with a 669 bp open reading frame (ORF) encoding a putative DRE/CRT (LTRE)-binding protein of 223 amino acids. The predicted CbCBF25 protein contained a potential nuclear localization signal (NLS) in its N-terminal region followed by an AP2 DNA-binding motif and a possible acidic activation domain in the C-terminal region. Bioinformatic analysis revealed that Cbcbf25 has a high level of similarity with other CBF genes like cbf1, cbf2, and cbf3 from Arabidopsis thaliana, and Bncbf5, Bncbf7, Bncbf16, and Bncbf17 from Brassica napus. A cold acclimation assay showed that Cbcbf25 was expressed immediately after cold triggering, but this expression was transient, suggesting that it concerns cold acclimation. Our study implies that Cbcbf25 is an analogue of other CBF genes and may participate in cold-response, by for example, controlling the expression of cold-regulated genes or increasing the freezing tolerance of plants.

• The Journal of Korean Association of Computer Education
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• v.5 no.2
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• pp.21-28
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• 2002

This paper proposes a new approach to rollback-recovery using multi-agent in distributed computing system. Previous rollback-recovery protocols are dependent on inherent communication and operating system, which causes a decline of computing performance in distributed computing system. By using multi-agent, we propose rollback-recovery protocol that is independent on operating system. We define three kinds of agent. One is a recovery agent that performs rollback-recovery protocol after a failure. Other is an information agent that constructs domain knowledge as a rule of fault tolerance and information during failure-free operation. The other is facilitator agent that controls the efficient communication between agents. Also we propose rollback-recovery protocol using multi-agent and simulated the proposed rollback-recovery protocol using JAVA and agent communication language in CORBA environment.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.77.2-78
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• 2003

An EREBP/AP2-type transcription factor (CaPFl) was isolated by DDRT-PCR following inoculation of soybean pustule pathogen Xanthomonas axonopodis pv. glycines Bra which induces HR on pepper leaves. Genomic Southern blot analysis revealed that the CaPFl gene is present as a single copy within the hot pepper genome. The deduced amino acid sequence of CaPFl has two potential nuclear localization signals, a possible acidic activation domain, and an EREBP/AP2 motif that could bind to a conserved cis- element present in promoter region of many stress-induced genes. The mRNA level of CaPFl was induced by both biotic and abiotic stresses. We observed higher-level transcripts in resistance-induced pepper tissues than diseased tissues. Expression of CaPFl is also induced upon various abiotic stresses including ethephon, MeJA, cold stress, drought stress and salt stress treatments. To study the role of CPFI in plant, transgenic Arabidopsis and tobacco plants which express higher level of pepper CaPFl were generated. Global gene expression analysis of transgenic Arabidopsis by cDNA microarray indicated that expression of CaPFl in transgenic plants affect the expression of quite a few GCC box and DRE/CRT box-containing genes. Furthermore, the transgenic Arabidopsis and tobacco plant, expressing CaPFl showed tolerance against freezing temperature and enhanced resistance to Pseudomonas syrnigae pv. tabaci. Taken together, these results indicated that CaPFl is a novel EREBP/AP2 transcription factor in hot pepper plant and it may has a significant role(s) in regulation of biotic and abiotic stresses in plant.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.2
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• pp.198-207
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• 2018

Objective: This study investigated the association of yeast species with improved aerobic stability of total mixed ration (TMR) silages with prolonged ensiling, and clarified the characteristics of yeast species and their role during aerobic deterioration. Methods: Whole crop corn (WCC) silages and TMR silages formulated with WCC were ensiled for 7, 14, 28, and 56 d and used for an aerobic stability test. Predominant yeast species were isolated from different periods and identified by sequencing analyses of the 26S rRNA gene D1/D2 domain. Characteristics (assimilation and tolerance) of the yeast species and their role during aerobic deterioration were investigated. Results: In addition to species of Candida glabrata and Pichia kudriavzevii (P. kudriavzevii) previously isolated in WCC and TMR, Pichia manshurica (P. manshurica), Candida ethanolica (C. ethanolica), and Zygosaccharomyces bailii (Z. bailii) isolated at great frequency during deterioration, were capable of assimilating lactic or acetic acid and tolerant to acetic acid and might function more in deteriorating TMR silages at early fermentation (7 d and 14 d). With ensiling prolonged to 28 d, silages became more (p<0.01) stable when exposed to air, coinciding with the inhibition of yeast to below the detection limit. Species of P. manshurica that were predominant in deteriorating WCC silages were not detectable in TMR silages. In addition, the predominant yeast species of Z. bailii in deteriorating TMR silages at later fermentation (28 d and 56 d) were not observed in both WCC and WCC silages. Conclusion: The inhibition of yeasts, particularly P. kudriavzevii, probably account for the improved aerobic stability of TMR silages at later fermentation. Fewer species seemed to be involved in aerobic deterioration of silages at later fermentation and Z. bailii was most likely to initiate the aerobic deterioration of TMR silages at later fermentation. The use of WCC in TMR might not influence the predominant yeast species during aerobic deterioration of TMR silages.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.17 no.1 s.104
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• pp.74-80
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• 2006

This paper presents the characteristics of electromagnetic field uniformity in a reverberation chamber that can be used alternatively for the analysis, test and evaluation of electromagnetic interference and immunity. The dual-band diffuser, which can be used at two different frequency bands in a reverberation chamber, is applied, and it is made of two different single-band Schroeder's Quadratic Residue Diffusers. The FDTD method is used to analyze the field characteristics. Compared with single-band diffuser, the dual-band diffuser shows the improvement in not only expansion of test frequency band but also in characteristics of the field uniformity, polarity, power efficiency, and tolerance. Therefore, the reverberation chamber with the dual-band diffuser is a better facility, for the analysis and measurement of electromagnetic interference and immunity, than the reverberation chamber with a single-band diffuser.

• Journal of the Institute of Electronics Engineers of Korea CI
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• v.45 no.6
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• pp.33-44
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• 2008

Multipath routing protocols with load balance, fault tolerance, aggregated bandwidth in Ad-hoc Networks provide improved throughput and reliable route as compared with singlepath routing protocols. However, multipath routing protocols have not been explored thoroughly in the domain of overhead in Ad-hoc Networks. In this paper, we analyze and compare on-demand singlepath and multipath routing with IEEE 802.11 DCF in terms of Routing overhead and MAC overhead. The results reveals that in comparison with singlepath routing protocol, multipath routing mechanism creates more overheads but provides better performance in congestion and capacity provided that the route length is within a certain upper bound which is derivable. The analytical results are further confirmed by simulation.

• Journal of the Korea institute for structural maintenance and inspection
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• v.14 no.6
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• pp.254-262
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• 2010

In order to evaluate vibration serviceability by means of non-contact vibration meter, serviceability of existing bridge were experimentally evaluated by using laser vibration meter. Test results were analyzed and compared with evaluation results acquired by using accelerometer and LVDT. To testify accuracy of laser vibration meter measured natural frequency were compared with that acquired by using accelerometer. According to test and comparison results it is showed that serviceability can be evaluated properly enough from the tolerance curve of Reiher-Meister for the estimated acceleration that can be calculated by the numerical differentiation of measured velocity. But because of cumulative numerical error occurred during integration of measured velocity in the time domain serviceability grade could be different from the result acquired by LVDT.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.18 no.5 s.120
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• pp.471-477
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• 2007

This paper presents the characteristics of electromagnetic field uniformity in a reverberation chamber with flat diffusers composed of multiple dielectric materials that can be used as an alternative facility for the analysis, test and evaluation of electromagnetic interference and immunity. The field characteristics and the size of the test volume inside the reverberation chamber with the new diffuser of multiple dielectric materials are simulated and analyzed at $2.5{\sim}3$ GHz band. The FDTD method is used to analyze the field characteristics. The field uniformity, polarization characteristics and tolerance are improved by the new diffuser with smaller physical size. The reverberation chamber with flat diffusers composed of multiple dielectric materials shows better electromagnetic performance and larger test volume than normal chamber.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.4
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• pp.432-440
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• 2019

Global climate change exerts adverse effects on maize production. Among abiotic stresses, drought stress during the tasseling stage (VT) can increase anthesis-silking intervals (ASI) and decrease yield. We performed an evaluation of ASI and yield using a drought-sensitive line (Ki3) and a drought-tolerant line (Ki11) to analyze the correlation with ASI and yield. Moreover, the de novo data of Ki11 were analyzed to find putative novel transcripts related todrought stress in tropical maize. A total of 182 transcripts, with a log2 ratio >1.5, were found by comparing drought conditions to a control. The top 40 transcripts of high expression levels in the de novo analysis were selected and analyzed with PCR. Of the 40 transcripts, six novel transcripts were detected by quantitative real-time PCR (qRT-PCR) using seedling and VT stage samples. Five transcripts (transcripts_1, 12, 34, 35, and 40) were up-regulated in the Ki11 shoot at seedling stage, and transcripts_1, 12, and 40 were up-regulated at the re-watering stage after 12 h of drought stress. The transcripts_32 and 34 were up-regulated at the VT stage. Hence, transcript_34 possibly plays a significant role in drought tolerance during the seedling and VT stages. The transcript_32 was identified as chloramphenicol acetyltransferase (CAT) by Pfam domain analysis. The function of the other transcripts remained unknown. Further characterization of these novel transcripts in genetic regulation will be of great value for the improvement of maize production.

• Plant Breeding and Biotechnology
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• v.5 no.2
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• pp.123-133
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• 2017

AP2/EREBP gene family consists of transcription factor genes with a conserved AP2 DNA-binding domain and is involved in various biological processes. AP2/EREBP gene families were identified through genome-wide searches in five Cucurbitaceae species including cucumber, wild cucumber, melon, watermelon, and bitter gourd, which consisted of more than 100 genes in each of the five species. The gene families were further divided into five groups including four subfamilies (ERF, DREB, AP2 and RAV) and a soloist group. Among the subfamilies, DREB subfamily which is known to be related to abiotic stress response was more analyzed and a total of 25 genes were identified as Cucurbitaceae homologues of Arabidopsis CBF/DREB1 genes which are important for abiotic stress-response and tolerance. In silico expression profiling using RNA-Seq data revealed diverse expression patterns of cucumber AP2/EREBP genes. AP2/EREBP gene families identified in this study will be valuable for understanding the stress response mechanism as well as facilitating molecular breeding in Cucurbitaceae crops.