• Title/Summary/Keyword: tolclofos-methyl

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Extraction of pesticide residues in medical herbs by microwave (Microwave를 이용한 한약재 중의 잔류농약 추출)

  • Kim, Taek-Kyum;Kim, Tang-Eok
    • The Korean Journal of Pesticide Science
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    • v.4 no.3
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    • pp.60-67
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    • 2000
  • This study was conducted to elucidate extraction efficiency by microwave in comparison with Soxhlet for extraction of pesticide residues in dried medical herbs; red-ginseng, white-ginseng, Bupleuri Radix, Angelica gigas Nakai, Rehmannia glutinosa. The acetone extraction by microwave of tolclofos-methyl and quintozene in medical herbs was efficient. The extraction efficiency by microwave with power 45 to 150 watts, extraction time 1 to 5 minutes and solvent volume 30 ml was compared with that of Soxhlet with extraction time 7 hours and solvent volume 150 ml. The extraction efficiency by microwave with extraction time 3 to 5 minutes was similar with extraction time of 7 hours by Soxhlet. When medical herbs spiked with tolclofos-methyl and quintozene was analyzed to how the extraction efficiency of microwave by kind of medical herbs, the extraction efficiency by microwave with extraction time of 3 to 5 minutes was the same as Soxhlet extraction. The optimal condition for extraction of tolclofos-methyl and quintozene in medical herbs by microwave was 45 to 90 watts of power supply, 3 to 5 minutes of extraction time and acetone 30 ml of solvent volume.

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Effect of Aqueous Solutions of Pesticides on Survival of Entomopathogenic Nematodes (농약이 곤충병원성 선충의 생존에 미치는 영향)

  • 이동운;추호렬;최은정
    • Asian Journal of Turfgrass Science
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    • v.13 no.4
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    • pp.213-222
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    • 1999
  • The toxic effects of four pesticides on the entomopathogenic nematodes. Heterorhabditis bacteriophora NC strain, Steinernema glaseri NC and Dongrae strain were tested by checking the mortality of infective juveniles(Ijs) in aqueous solution of pesticide. Mortality of Ijs was influenced by pesticide and concentration and soaking time. The herbicide alachlor and insecticide fenitrothion were toxic to Ijc of three entomopathogenic nematodes. But, the fungicides mepronil and tolclofos-methyl were nontoxic to Ijs. Tolclofos-methyl showed significantly very week toxicity at 28 days after soaking for S. glaseri NC strain. H. bacteriophora NC strain was more highly sensitive to high temperature condition then were the other nematodes.

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Effects of Pesticides on the Pathogenicity of Entomopathogenic Fungi, Beauveria bassiana and Metarhizium anisopliae (곤충병원성곰팡이 Beauveria bassiana와 Metarhizium anisopliae의 병원성에 미치는 농약의 영향)

  • 이상명;이동운;추호렬;박영도
    • Korean journal of applied entomology
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    • v.36 no.2
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    • pp.179-184
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    • 1997
  • This study was conducted to observe the effects of pesticides on the pathogenicity of entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae isolated from Korea. Spomlation of entomopathogenic fungi in SMAY medium that mixed different concentrations of pesticides was similar to control in metalaxyl and tolclofos- methyl treatment but in half recommanded treatment of fenitrothion and mepronil very poor spomlation was showed. Pathogenicity of entomopathogenic fungi dipped in all tested pesticide solution for 1 to 5hr was 100% pathogenicity of M. aniropliae in the pesticide treated chinese cabage fields were similar to control, Bt, teflubenzuron, metalaxyl, and carbofuran treatment but alachlol was below 80%. Pathogenicity and persistence of entomopathogenic fungi in turfgrass when pesticides were posttreatmented were continued to 4 months in iprodione+thiram and tolclofos-methyl treated plots but those of B. bassiana and M. anisopliae was losted in fenitrothion pathogenicity and persistence of in mepronil were nil M. anisopliae from 3 months after treatment.

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Residues of Tolclofos-methyl, Azoxystrobin and Difenoconazole in Ginseng Sprayed by Safe Use Guideline (안전사용기준에 따라 살포된 살균제 Tolclofos-methyl, Azoxystrobin 및 Difenoconazole의 인삼 중 잔류량)

  • Kim, Jang-Eok;Kim, Tae-Hwa;Kim, Young-Hwan;Lee, Ju-Hee;Kim, Jin-Su;Paek, Seung-Kyoung;Choi, Su-Yeon;Youn, Young-Nam;Yu, Yong-Man
    • Korean Journal of Medicinal Crop Science
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    • v.16 no.6
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    • pp.390-396
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    • 2008
  • The aim of this work is to assess the safety of fungicide tolclofos-methyl, difenoconazole and azoxystrobin in ginseng sprayed by safe use guideline. When tolclofos-methyl was sprayed on ginseng by safe use guideline, the residue amounts (MRL) of it in ginseng was 0.13 mg/kg which is below than 0.3 mg/kg, maximum residue limit established by Korea Food & Drug Administration (KFDA). The residue amounts of ginseng parts, head part was 0.37 mg/kg and main body part was 0.13 mg/kg. In case of difenoconazole, the residue amounts in ginseng was 0.81 mg/kg. which was exceed the MRL, 0.2 mg/kg. By the analyze results of ginseng part, the residues of head and main body part were 3.01 and 0.40 mg/kg, respectively. In experiment of vinyl mulching, the residue amount of difenoconazole in ginseng was 0.05 mg/kg. The residue amounts of azoxystrobin in ginseng sprayed by safe use guideline was 0.14 mg/kg. This residue was not exceed the MRL 0.5 mg/kg. The residue amounts by ginseng parts was 0.51 mg/kg for head part and 0.28 mg/kg for main body part. In case of vinyl mulching, the residue amount of azoxystrobin was 0.02 mg/kg.

Screening of Seed Treatment Fungicide for Control of Damping-off caused by Rhizoctonia solani on Panax ginseng (Rhizoctonia solani에 의한 인삼 모잘록병 방제용 종자처리 약제 선발)

  • Shin, Jeong-Seop;Lee, Seung-Hwan;Cho, Hye-Sun;Cho, Dae-Hui;Kim, Kyoung-Ju;Hong, Tae-Kyun;Park, Chol-Soo;Lee, Seong-Kye;Jung, Hee-Young
    • The Korean Journal of Pesticide Science
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    • v.19 no.4
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    • pp.424-427
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    • 2015
  • Damping-off is a critical disease on ginseng seedling, which caused by the fungal pathogen Rhizoctonia solani. The disease has been prevented by tolclofos-methyl for the last 20 years. However, the tolclofos-methyl usually detected on the harvested roots of 6-year-old ginseng. Herein, we tried to select an alternative pesticide which not only must be safe but also efficiently inhibits the fungal pathogen. Four fungicides (fludioxonil, flutolanil, pencycuron, and thifluzamide) were applied to their inhibition efficacy against the pathogen. In in vitro test, fludioxonil treatment showed 80% inhibition activity for 25 days. Thifluzamide and flutolanil showed the activity for 10 days. Pencycuron showed for 1 days. In addition, the fludioxonil was more effective to control the pathogen comparing to other three fungicides in field. The incidence of damping-off was reduced to 71% by fludioxonil treatment. The level of the fungicide residue in seedling was 0.44 mg/kg, which value will be a negligible level in final products after 5 years. Consequently, the fludioxonil is a conceivable alternative for tolclofos-methyl to cope with R. solani.

Monitoring and Safety Assessment of Pesticide Residues in Ginseng (Panax ginseng C.A. Meyer) from Traditional Markets (유통 수삼 중 잔류농약 모니터링 및 안전성 평가)

  • Noh, Hyun Ho;Lee, Jae Yun;Park, Hyo Kyoung;Jeong, Hye Rim;Lee, Jeong Woo;Jin, Me Jee;Choi, Hwang;Yun, Sang Soon;Kyung, Kee Sung
    • The Korean Journal of Pesticide Science
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    • v.20 no.1
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    • pp.23-29
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    • 2016
  • This study was carried out to survey residual characteristics of pesticide in fresh ginsengs collected from 45 markets at 15 regions in Korea using multiresidue analysis with a GC-MS/MS and an LC-MS/MS. After residue analysis was performed, the pesticides detected from ginsengs were quantitated using their analytical methods validated by recovery tests with a GC-ECD/NPD. As a results of analysis of pesticide residue, cypermethrin, fenitrothion, fludioxonil, thifluzamide, and tolclofos-methyl were detected from 16 samples among 45 samples in total, indicating detection rate was 35.6%. Tolclofos-methyl was found to be highest in detection frequency in ginseng. Fenitrothion that has not established maximum residue limit and pre-harvest interval for ginseng was detected. The amounts of all pesticides detected were less than their MRLs. Ratios of estimated daily intakes to acceptable daily intakes of the detected pesticides in ginseng were found to be from 0.03 to 16.67%.

Development of Fermentation Process of Ginseng Leaf Extraction Probiotic Strain and Characterization of Product Quality (프로바이오틱 균주에 의한 인삼 잎 추출물 발효공정 확립 및 생성물의 품질 특성분석)

  • Hur, Sang-Sun
    • Journal of the Korean Applied Science and Technology
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    • v.35 no.4
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    • pp.1213-1223
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    • 2018
  • This study was carried out to investigate extraction efficiency by microwave for extraction of pesticide residues and the bioconversion of ginsenosides of ginseng leaf by using various lactic acid bacteria in order to promote the utilization of ginseng leaf. The hexane extraction by microwave of tolclofos-methyl and azoxystrobin in ginseng leaf was efficient. The optimal condition for extraction of tolclofos-methyl and azoxystrobin in ginseng leaf by microwave was 50 to 95 watts of power supply, 3 minutes of extraction.The gisenosides Rg1 and Rb1 contents have decreased, while the Rh1, Rg3, Rk1 and Rh2 have increased due to fermentation. The ginsenosides Rg3 of the fermented ginseng leaf has increased and the contents were $70.62{\sim}77.61{\mu}g/g$(control $2.77{\mu}g/g$). The total phenolic acid content and electron donating ability of the ginseng leaf have totally decreased after 7 days of fermentation. The total phenolic acid contents of the fermented ginseng leaf with various lactic acid bacteria did not show any tendency as different strains.

Risk Assessment of Pesticide for Earthworms (농약의 지렁이에 대한 위해성 평가)

  • Park, Kyung-Hun;Park, Yeon-Ki;Joo, Jin-Bok;Kyung, Kee-Sung;Shin, Jin-Sup;Kim, Chan-Sub;Park, Byung-Jun;Uhm, Jae-Youl
    • The Korean Journal of Pesticide Science
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    • v.7 no.4
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    • pp.280-287
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    • 2003
  • To assess the risk of pesticides on earthworm, the acute toxicities of 10 pesticides were investigated and their toxicity exposure ratios(TERs) were calculated. As the TERs of paraquat dichloride and pendimethalin were more than 100, their risks were rated negligible. Risk of benfuracarb, cadusafos, chlorpyrifos-methyl, endosulfan, isazofos and parathion which have TERs of $10\sim100$ were rated low. However, risk of imidacloprid and phorate which have TER of less than 10 were estimated highly to need a reproduction study. Earthworms were exposed to twenty two pesticides including dazomet 98% GR having PECs of more than $5mg{\cdot}kg^{-1}$ in artificial soil at standard and double dose for 14 days. All the earthworms exposed to dazomet 98% GR and metam-sodium 25% SL were died to show their high risk, while no serious adverse effects were observed in the soil treated with 15 pesticides, calcite 95% WP, calcium polysulfide 36% CF, chlorothalonil 75% WP, daminozide 85% WP, dichlonil 6.7% GR, etridiazole 25% EC, fosetyl-Al 80% WP, glyphosate 41 % SL, hymexazol 30% SL, iprodione 50% WP, machine oil 95% EC, mancozeb 75% WP, propineb 70% WP, terbuthylazine 80% WP and triazophos 40% EC. In case of thiophanate-methyl 70% WP, copper hydroxide 77% WP, dimethoate 46% EC, tolclofos-methyl 50% WP and propamocarb hydrochloride 67% SL, any effect did not show clearly, suggesting an additional subchronic toxicity study. The risk of thiophanate-methyl 70% WP to earthworm was estimated high, considering its subchronic effect, while effects of copper hydroxide 77% WP, dimethoate 46% EC, tolclofos-methyl 50% WP and propamocarb hydrochloride 67% SL to earthworms were negligible, considering no adverse effects in subchronic tests.

Chemical Control of White and Violet Root Rot Caused by Rosellinia necatrix and Helicobasidium mompa on Apple Tree (사과나무 흰날개무늬병과 자주날개무늬병의 약제 방제)

  • 이상범;정봉구;김기홍;최용문
    • Plant Disease and Agriculture
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    • v.1 no.1
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    • pp.37-44
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    • 1995
  • This study was carried out to select effective fungicides against white and violet root rot caused by Rosellinia necatrix and Helicobasidium mompa with nine fungicides including thiophanate-methyl from 1993 to 1994. Through laboratory, greenhouse and field trials on inhibitory effect of mycelial growth and disease incidence against the two fungal pathogens, 5 fungicides have been selected finally. Thiopanate-methyl, benomyl, iminoctadine-triacetate and isoprothiolane were proven to have high control effect against R. necatrix. In addition to thiopanate-methyl and benomyl, tolclofos-methyl has been selected for effective control of H. mompa, since it showed prominent control effect in field trial than in laboratory or green house test.

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Evaluation of Residual Pesticides in Fresh Ginseng Collected in Seoul

  • Kim, Tae-Rang;Park, Ki-Hwan;Jang, Mi-Ra;Choi, Young-Hee;Kim, Eun-Hee;Choi, Chae-Man;Park, Sung-Kyu;Yu, In-Sil;Hwang, In-Sook;Han, Ki-Young;Kim, Moo-Sang;Kim, Jung-Hun;Chae, Young-Zoo
    • Journal of Applied Biological Chemistry
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    • v.56 no.1
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    • pp.29-35
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    • 2013
  • This study was performed to analyze 48 kinds of pesticide residues using gas chromatography (GC)/nitrogen phosphorous detector, GC/micro electron capture detector, GC/mass selective detector, and high performance liquid chromatograph/diode array detector in 186 fresh ginseng samples collected in the Seoul area from 2010 to 2011. Fresh ginseng dietary intakes were estimated using the data from the 2009 Korea National Health and Nutrition examination survey. Residual pesticides were detected in 79 samples (42.5%) with eight different fungicides. Only 20 samples (10.8%) exceeded the maximum residue limits (MRLs) for pesticides registered by the Korea Food & Drug Administration. Among them, tolclofos-methyl residues (10.2%) exceeded the MRL for fresh ginseng in 18 ginseng seedlings and one of the two-year old fresh ginseng plants, and the residual level in just one ginseng seedling violated the MRL for pyrimethanil. The results showed that residual pesticides levels in marketable fresh ginseng around Seoul were relatively safe. The percent acceptable daily intake (%ADI) was calculated using pesticide residues in fresh ginseng and dietary intakes of fresh ginseng. The risk caused by pesticide residues in fresh ginseng was very low.