• 한국연초학회지
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• 제22권1호
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• pp.45-50
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• 2000

In order to improve the sowing practice, pelleted seeds of tobacco NC 82(Nicotiana tobacco L.) were manufactured in use of binders at the different levels of viscosity, and their physical properties according to pellet size and biological activity in seed germination were investigated while storage time elapsed. Proper range of the binder viscosity for the pellet formation was 20.3-37.2 m.pas. At the high level of viscosity(45.7 m.pas) took longer time to form the pellet and pelleting was not easy. The high binder viscosity and large pellet size revealed higher level in hardness of the pelleted tobacco seeds, which caused the longer splitting time of pellets in water. High level of binder viscosity(37.2 m.pas) in pelleted seeds led to decrease the biological activity of tobacco seed germination by the storage at 4 t over 30 days. But at the level of 20.3m.pas in binder viscosity, the germination of pelleted seeds was little affected by the long storage time to 120 days.

• 한국연초학회지
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• 제24권2호
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• pp.82-87
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• 2002

The purposes of this study are to investigate water content and treatment period in Solid Matrix Priming(SMP; method for enhanced rapid and uniform seedling emergence in tobacco seeds). The higher water content made the faster imbibition speed of tobacco seed. But the radicle emergence shorten the period of water equilibrium. The range of water content in SMP treatment within the limits of no radicle emergence of tobacco seed was 28∼32% to dry weight of tobacco seed. The rate of radicle emergence and seedling emergence was higher in water content 30% and 40% treatment plot than that in 50% and 60% treatment plot. But it was not different between SMP treatment and non-SMP treatment. In water content 40% during 9 days treatment, germinative energy was highest, and T50 and mean germination time(MGT) were the shortest. The germination speed was higher in SMP treatment than that in non-SMP treatment. It was not different on the germination rate between non-SMP treatment and SMP treatment.

• 한국연초학회지
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• 제18권2호
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• pp.126-131
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• 1996

In this study effects of isolation distance, transplanting time of maternal plants, and bagging of flower head with the gauze-cloth bag on the outcrossing of burley tobacco (Nicotiana tabacum L.) were investigated. Also the effect of fertilizer level and control of the number of capsules per plant on seed production and quality were examined. A male sterile line. produced 0.3 to 3.8 capsules Per plant when it was Planted with normally flowering tobacco with the average outcrossing of 7.2 plants, ranging from 2 to 18 out of 20 plants. With the farther the isolation distance between maternal plants and pollen donor plant, the lower the outcrossing occurred. Outcrossing occurred even at the isolation distance of 312 m. When the maternal plants were transplanted 35 days after transplanting the pollen donor ones, the outcrossed plants were not decreased significantly. The bagging of the flower head with the gauze-cloth bas (#0.9∼ 1.0 mm) decreased the outcrossed plants significantly, but couldn't prevent the outcrossing completely. The seed amount per plant was higher in the highly fertilized cultivation. The number of seed capsules per plant affected significantly on seed yield and quality. When the seed capsules was controlled by 30 or 50 capsules per plant, the weight of 1,000 seeds and germination rate were higher than those with 70 or 90 capsules per plant. Key words : Nicotiana tabacum, outcrossing, bagging.

• Applied Biological Chemistry
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• 제35권4호
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• pp.272-275
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• 1992

한국산 녹차 종자(Camellia sinecis L.)에 함유된 단백질, 탄수화물, 조지방, 조회분 함량은 해바라기(Helianthus annuus)씨나 잇꽃종실(Carthamus tinctorius L.) 등과 대등하였으나, 조사포닌은 12.2%로 참깨(0.29%)나 땅콩(0.63%)보다 월등히 높게 함유하였고, oleic acid를 비롯한 불포화 지방산 조성은 82%였다. Tocopherol은 ${\alpha}-form$만 $22\;{\mu}g/g$ 보였을 뿐 일반종자보다 현저히 낮은 수준으로 함유하였다.

• Journal of Ginseng Research
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• 제5권2호
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• pp.85-91
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• 1981

Ginseng seeds were gathered from 3,4,5 and 6 years of age and were classified into four qroups (below 4mm, 4∼5mm, 5∼6mm and above 6mm in across sieve). They were sown in seedling bed and some characters were investigated in each qroup of seed size. 1. The distribution of seed size of below 4mm, 4-5mm, 5-6mm and 6mm were 23.7%, 60.8%, 12.4% and 4.5%, respectively. 2. The ratio of seed coat dehiscence was not affected by seed size but emergence ratio and emerging vigor were superior in large seed. 3. The large seed showed superiority in stem length, stem diameter, leat and also in root length, root diameter and root weight. but diseased root was not affected by seed size. The effect of age(seed harvest) was not significant on all those characters.

• 한국연초학회지
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• 제21권2호
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• pp.152-159
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• 1999

A CAB cDNA clone(pKGCAB) encoding the light harvesting chlorophyll a/b binding protein of the semi-shade plant, Korean ginseng(Panax ginseng C. A. Meyer) was isolated by the one-way path random sequencing of ginseng cDNA library clones and transgenic tobacco plants(Nicotiana tabacum NC82) were produced by the transformation of this ginseng CAB gene in use of Agrobacterium tumefaciens LBA4404. The CAB gene showed type 1 structure of LHCP-II, 84% similarity in nucleotide sequence and 92% in amino acid sequence to that of Nicotiana tabacum CAB40, respectively. Seed germination and initial growth of the transgenic tobacco plants transformed with the cDNA fragment were accelerated under low light intensity compared with those of normal tobacco plant, that may result from the higher light sensitivity of the transgenic plants than that of the normal.

• 한국작물학회지
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• 제31권1호
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• pp.19-23
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• 1986

27개 농가포장에서 인삼종자의 생산량 및 종자소질을 조사하였던 바, 그 결과를 요약하면 다음과 같다. 1. 포장별 채종량은 거의가 4~10$\ell$/간(간 : 180x90cm의 면적) 절위이였다. 2. 채종량이 많은 포장의 종자일수록 100입중, 4mm이하의 종자 생산 비율이 모두 낮아지는 경향이었다. 3. 전종자의 12%는 4mm이하의 종자이었으며 66%는 4-5mm, 22%는 5mm이하의 종자이었다. 4. 채종모본 선정 포장에서는 방임채종포에 비해 채종량은 감소되었으나 100입중, 4mm이하 종자의 생산비율은 오히려 증가되었다. 5. 종자의 길이와 폭 간에는 정(＋)상관이 인정되었으나 폭과 두께, 길이와 두께간에는 유의상관 인정되지 않았다. 6. 채종량은 채종모본의 생육상태에 따라 차이가 있었으나 4mm이하의 종자생산 비율을 감안할 때 100간당 7-8$\ell$ 미만 채종하는 것이 좋을 것으로 보여졌다.

• 한국연초학회지
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• 제20권1호
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• pp.50-56
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• 1998

The complementary DNA (cDNA) of potato virus Y- vein necrosis strain (PVY-VN) replicase gene (Nlb) was transformed into tobacco (Nicotiana tabacum cv. Burley 21) plants. Out of 25 putative transformants regenerated, 3 were resistant to PVY-VN, one highly resistant plant with no symptom until seed harvest time and the other two with mild chlorotic spot symptoms at late stages after infection. No symptom was observed in the highly resistant plant, while mild vein necrotic symptoms were developed on suckers of the moderately resistant plants after seed harvest time, In the first generation (T1) via self fertilization, resistance to susceptibility frequency in transgenic plants from the highly resistant transformant was about 3 : 1, while it was lowered much (about 1:2 and 1:19) in T1 of the moderately resistant transformants. In the second generation (T2) of the highly resistant plant, resistance frequencies were similar to T1, but resistance levels varied greatly and appeared to be decreased. Key words : potato virus Y, viral replicate gene, transgenic tobacco plants, resistance.

• 한국연초학회지
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• 제20권2호
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• pp.166-171
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• 1998

In order to improve the efficiency of F$_1$ hybrid seeds production(KF 114), some experiments were carried out in a greenhouse this year. Mother plant(MSNC567) and pollen plant(NC) were grown in some pots (30 x 30cm, WxH). The gathered pollens were mixed with celite, pollen deluent dusts and stored in refrigerator at l$0^{\circ}C$ before pollination. To establish the critical range of the mixed rate and the storage period of pollens, the change in a percent of capsule set, number of seed and seed weight per capsule, weight of 1000 seeds and germination percent of seed resulted of pollination with pollen deluent dusts were investigated. The results are as follows; Percent of capsule set showed the mixed rate 1:5 resulted in no difference and those of 1:10 and 1:20 decreased about 5 ～15% when compared with trials using pollen alone. There was no difference among duration of storage in a same mixed rate. Numbers of seed per capsule showed a significant difference among the mixed rates and among durations of storage. Numbers of seed per capsule were decreased about 55 ～ 90 % as the mixed rate increase, about 2 ～ 28 % as the duration of storage increase. Weight of seed per capsule decreased about 16 ～ 23 % as the duration increase and decreased about 3 ～ 89 % as the mixed rate increase when compared with trials using pollen alone. Weight of 1000 seeds showed non significant difference among duration of storage but were higher than trials used pollen alone as the mixed rate increase.

• 한국작물학회지
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• 제28권2호
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• pp.262-266
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• 1983

인삼종자의 내과피에 부생하는 진균이 배생장 및 개압에 미치는 영향을 알기 위하여 종자와 층적장의 모래를 살균 처리한 뒤 종자를 층적하여 얻은 결과를 요약하면 다음과 같다. 1. 종자소독에 의해 종자의 내과피에 부생하는 균밀도가 현저히 저하되었으며 종자의 경도변화가 크게 억제되었고, 종자의 경도와 균밀도간에는 부(-)상관 $(r=0.984^**)$이 인정되었다. 2. 종자내 수분함량의 증가는 종자소독구가 대조구보다 20일 늦게 시작되었다. 3. 배생장과 개압은 종자소독에 의해 크게 억제되었고 종자의 경도가 낮을수록 배생장은 잘되었다. 인삼종자의 내과피에 부생하는 진균이 내과피를 경화시켜 산소, 수분 등의 공급을 쉽게 함으로써 배생장 및 개갑을 촉진시키는 것으로 생각된다.