• Molecules and Cells
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• 제27권1호
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• pp.47-54
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• 2009

A cDNA clone for a transcript preferentially expressed during an early phase of flooding was isolated from Nicotiana tabacum. Nucleotide sequencing of the cDNA clone identified an open reading frame that has high homology to the previously reported glycine-rich RNA-binding proteins. The open reading frame consists of 157 amino acids with an N-terminal RNA-recognition motif and a C-terminal glycine-rich domain, and thus the cDNA clone was designated as Nicotiana tabaccum glycine-rich RNA-binding protein-1 (NtGRP1). Expression of NtGRP1 was upregulated under flooding stress and also increased, but at much lower levels, under conditions of cold, drought, heat, high salt content, and abscisic acid treatment. RNA homopolymer-binding assay showed that NtGRP1 binds to all the RNA homopolymers tested with a higher affinity to poly r(G) and poly r(A) than to poly r(U) and poly r(C). Nucleic acid-binding assays showed that NtGRP1 binds to ssDNA, dsDNA, and mRNA. NtGRP1 suppressed expression of the fire luciferase gene in vitro, and the suppression of luciferase gene expression could be rescued by addition of oligonucleotides. Collectively, the data suggest NtGRP1 as a negative modulator of gene expression by binding to DNA or RNA in bulk that could be advantageous for plants in a stress condition like flooding.

• The Plant Pathology Journal
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• 제23권2호
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• pp.76-89
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• 2007

CaCDPK4, a full-length cDNA clone encoding Capsicum annuum calcium-dependent protein kinase 4, was isolated from chili pepper (Capsicum annuum L.). Deduced amino acid sequence of CaCDPK4 shares the highest homology with tobacco NpCDPK8 and chickpea CaCDPK2 with 79% identity. Genomic blot analyses revealed that CaCDPK4 is present as a single copy in pepper genome, but it belongs to a multigene family. CaCDPK4 was highly induced when pepper plants were inoculated with an incompatible bacterial pathogen. Induced levels of CaCDPK4 transcripts were also detected in pepper leaves by the treatment of ethephon, an ethylene-inducing agent, and high-salt stress condition. The bacterial-expressed GST-CaCDPK4 protein showed to retain the autophosphorylation activity in vitro. GUS expression driven by CaCDPK4 promoter was examined in transgenic Arabidopsis containing transcriptional fusion of CaCDPK4 promoter. GUS expression under CaCDPK4 promoter was strong in the root and veins of the seedlings. GW (-1965) and D3 (-1377) promoters conferred on GUS expression in response to inoculation of an incompatible bacterial pathogen, but D4-GUS (-913) and DS-GUS (-833) did not. Taken together, our results suggest that CaCDPK4 can be implicated on signal transduction pathway of defense response against an incompatible bacterial pathogen in pepper.

• 식물병연구
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• 제23권4호
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• pp.379-382
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• 2017

2016년 3월, 모자이크와 황화 병징을 보이는 명월초 잎에서 CMV를 분리하여 CMV-Gyp로 명명하였다. 기주식물의 생물적 반응과 RT-PCR, PCR-RFLP, 이동단백질 및 외피단백질 유전자의 염기서열 분석을 통해 CMV를 동정하였다. 가지과(담배, 고추, 꽈리)와 비름과(흰명아주, 붉은명아주)에서는 전형적인 CMV의 병징이 나타났으나 박과(쥬키니호박, 오이)에서는 병징이 발현되지 않는 특성을 보였다. 3a 및 CP 유전자의 계통학적 분석 결과, CMV-Gyp가 CMV Subgroup II에 속하는 것으로 나타냈다. CMV-Gyp의 이동단백질과 외피단백질유전자의 서열은 아미노산 수준에서 CMV-Hnt와 99.3% 및 100% 일치하였다. 이 논문은 명월초에서 CMV 감염에 대한 첫 보고이다.

• Plant Biotechnology Reports
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• 제4권2호
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• pp.165-172
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• 2010

Genes that are expressed early in specific response to high salinity conditions were isolated from rapeseed plant (Brassica napus L.) using an mRNA differential display method. Five PCR fragments (DD1.5) were isolated that were induced by, but showed different response kinetics to, 200 mM NaCl. Nucleotide sequence analysis and homology search revealed that the deduced amino sequences of three of the five cDNA fragments showed considerable similarity to those of ${\beta}$-mannosidase (DD1), tomato Pti-6 proteins (DD5), and the tobacco harpin-induced protein hin1 (DD4), respectively. In contrast, the remaining clones, DD3 and DD2, did not correspond to any substantial existing annotation. Using the DD3 fragment as a probe, we isolated a full-length cDNA clone from the cDNA library, which we termed BnSWD1 (Brassica napus salt responsive WD40 1). The predicted amino-acid sequence of BnSWD1 contains eight WD40 repeats and is conserved in all eukaryotes. Notably, the BnSWD1 gene is expressed at high levels under salt-stress conditions. Furthermore, we found that BnSWD1 was upregulated after treatment with abscisic acid, salicylic acid, and methyl jasmonate. Our study suggests that BnSWD1, which is a novel WD40 repeat-containing protein, has a function in salt-stress responses in plants, possibly via abscisic acid-dependent and/or -independent signaling pathways.

• 한국작물학회지
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• 제33권1호
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• pp.64-69
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• 1988

보리(Hordeum vulgare)의 유묘이 $^{14}$ co$_2$를 10분 및 30분간 처리한 후 잎을 채취하여 아미노산, 당, 인산염 및 유기산과 같은 수용성 대사물질을 분석하였다. 배양기간에 따른 label 정도는 비슷하였으나 종류와 양에 있어서는 차이가 있었다. 10분 처리시에는 아미노산 분획보다 중성분회(당, 인산염에스퇴르 및 유기산)에서 label 양이 4.5배 증가하였으나 30분 처리시에는 1.7배로 감소하였다. 당분획에 있어서는 처리 시간이 길어짐에 따라 label 양이 약간 증가 하였으나 아미노산 분획에 있어서는 양과 질적으로 label 양이 현저히 증가하였다. 탄소 동화율은 10분에서 30분으로 길어질수록 현저히 감소하였다(0.125대 0.034와 0.042 mole $CO_2$/mg 엽록소/분).

• Journal of Microbiology and Biotechnology
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• 제26권10호
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• pp.1755-1764
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• 2016

The application of Bacillus sp. in the biological control of plant soilborne diseases has been shown to be an environmentally friendly alternative to the use of chemical fungicides. In this study, the effects of bioorganic fertilizer (BOF) fortified with Bacillus amyloliquefaciens SQY 162 on the suppression of tomato bacterial wilt were investigated in pot experiments. The disease incidence of tomato wilt after the application of BOF was 65.18% and 41.62% lower at 10 and 20 days after transplantation, respectively, than in the control condition. BOF also promoted the plant growth. The SQY 162 populations efficiently colonized the tomato rhizosphere, which directly suppressed the number of Ralstonia solanacearum in the tomato rhizosphere soil. In the presence of BOF, the activities of defense-related enzymes in tomato were lower than in the presence of the control treatment, but the expression levels of the defense-related genes of the plants in the salicylic acid and jasmonic acid pathways were enhanced. It was also found that strain SQY 162 could secrete antibiotic surfactin, but not volatile organic compounds, to suppress Ralstonia. The strain could also produce plant growth promotion compounds such as siderophores and indole-3-acetic acid. Thus, owing to its innate multiple-functional traits and its broad biocontrol activities, we found that this antagonistic strain isolated from the tobacco rhizosphere could establish itself successfully in the tomato rhizosphere to control soilborne diseases.

• Applied Biological Chemistry
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• 제37권6호
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• pp.503-508
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• 1994

17종과 45종 한방 식물체 메탄올 추출물의 배추좀나방과 담배거세미나방 유충에 대한 살충활성 및 섭식저해활성을 잎침지법으로 조사하였다. 살충활성은 8,000 ppm에서 조사하였는바, 현호색 추출물만이 담배거세미나방 유충에 대해 강한 살충활성을 보였다. 섭식저해활성은 5,000 ppm에서 조사한 결과, 누리장나무, 거문누리장나무, 목향, 우방자, 인진, 전호, 백지 및 현호색 추출물이 강한 섭식저해활성을 나타내었다.

• BMB Reports
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• 제40권6호
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• pp.845-852
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• 2007

The highly evolved flowers of orchids have colorful sepals and fused columns that offer an opportunity to discover new genes involved in floral development in monocotyledon species. In this investigation, we cloned and characterized the homologous PISTALLATA-like (PI-like) gene PhPI15 ($\underline{Ph}alaenopsis$ $\underline{PI}$ STILLATA # $\underline{15}$), from the Phalaenopsis hybrid cultivar. The protein sequence encoded by PhPI15 contains a typical PI-motif. Its sequence also formed a subclade with other monocot PI-type genes in phylogenetic analysis. Southern analysis showed that PhPI15 was present in the Phalaenopsis orchid genome as a single copy. Furthermore, it was expressed in all the whorls of the Phalaenopsis flower, while no expression was detected in vegetative organs. The flowers of transgenic tobacco plants ectopically expressing PhPI15 showed male-sterile phenotypes. Thus, as a Class-B MADS-box gene, PhPI15 specifies floral organ identity in orchids.

• The Plant Pathology Journal
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• 제22권2호
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• pp.119-124
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• 2006

Kudzu vine(Pueraria montana var. lobata) is an invasive climbing woody vine that envelops trees and shrubs, pressing physically and shutting out sunlight, which needs to be controlled. Kudzu vine pathogens were surveyed as a way to seek its biocontrol agents in 2002. Occurrence of a bacterial halo blight disease of kudzu vine was observed at several localities in Korea including Euiwang and Suwon in Gyeonggi Province, Daejon, and Gochang and Buan in Jeonbuk Province. Symptoms of brown to black spots with a surrounding yellowish halo appeared from June and lasted till the rainy season without much expansion, but accompanying often leaf blight and defoliation. Isolated bacteria were identified as Pseudomonas syringae pv. phaseolicola based on physiological and cultural characteristics, Biolog, fatty acid and 16S rDNA sequencing analyses. In artificial inoculation test, these bacteria produced the same halo spot symptoms on kudzu vine and bean plants. They also induced hypersensitive responses (HR) on tobacco, tomato, and chili pepper leaves. This is the first report of a bacterial disease of kudzu vine in Korea, and the bacterial pathogen can be used as a biocontrol agent against the pest plant.

• 미생물학회지
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• 제50권1호
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• pp.67-72
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• 2014

담배식물과 담배재배 토양으로부터 최소배지에 유일 탄소원으로 니코틴을 첨가한 배지(MB/N)를 이용하여 니코틴을 분해하는 새로운 균주의 분리를 시도하였다. 16S rRNA 유전자의 염기서열 분석과 표현형 시험 및 형태학적 시험으로 분리균주들은 Micrococcaceae 과의 Arthrobacter 속에 포함되는 균주로 판명되었다. NU15는 Arthrobacter nicotinovorans와 99.8%의 상동성을 보였고, NU11는 Arthrobacter equi와 98.2%의 상동성을 보여 신주일 가능성이 있었다. 두 균주 모두 양성의 간구균이며, catalase 양성, oxidase 음성이었다. 신주일 가능성이 있는 NU11균주의 니코틴 분해를 확인하기 위하여 MB/N 액체배지에서 배양하면서 니코틴 특이적으로 나타내는 260 nm에서의 흡광도가 감소를 측정한 결과, 니코틴이 균주에 의해 특이적으로 분해되는 것을 확인 할 수 있었으며, 분해균들은 니코틴 오염을 복원하는데 사용될 수 있을 것으로 생각된다.