• 한국연초학회지
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• 제6권2호
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• pp.169-178
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• 1984

This study was conducted to investigate the influence of the topping time and height on the nonvolatile organic and higher fatty acid contents and the correlation among acid contents of tobacco plants. Within the topping time, the flowering (normal) stage of topping was the lowest in total analyzed acid (TAA), total nonvolatile organic acid (TNOA) and magic acid contents of NC2326, however, the highest in those of Burley 21 on 30 days after budding. Total higher fatty acid (THFA) and linoleic acid contents tended to be low with normal stage of topping. The lower the topping height, the lower the TAA, THFA and linoleic acid contents in middle and upper leaves (or NC2326 and Burley 21 were observed. On the other hand, the lower the topping height, the lower the TNOA and malic acid contents in middle and upper leaves for Burley 21 and upper leaves for NC2326 were found. There was a significant positive correlation between magic acid and TNOA contents. Similarly, TAA content showed a significant positive correlation with TNOA, oxalic acid and citric acid contents. But there was a negative correlation between malic acid and THFA contents. There were highly positive correlations among oleic acid, linoleic acid and THFA contents, respectively.

• 한국응용곤충학회지
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• 제20권3호
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• pp.168-172
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• 1981

한국의 거세미류중에서 숯검은밤나방이 과연 우점종인가를 밝히고 그 가해기간 및 가해기의 유충령기를 조사하였다. $1979\~1980$년의 조사결과 숯검은밤나방유충이 봄철에 채집된 거세미류중 약 $80\%$ 이상을 차지하며 밭작물의 피해초기에는 대부분이 $5\~6$령충으로 가해기간은 약 $1\~l.5$개월이 됨을 알 수 있었다. 이 해충의 영명을 'dark grey cutworm'으로 명명하였다.

• 한국연초학회지
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• 제15권2호
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• pp.111-114
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• 1993

This study was carried out to obtain the basic information for breeding of aromatic burley tobacco, and to select the breeding lines with higher DVT (diterpenes $\alpha$-and $\beta$-4, 8, 13-duvatriene-1, 3-diols) and cis-abienol (Z-labda-12, 14-diene-8$\alpha$-ol) concentrations. The parental genotypes used to develop the Fl and Fl generations were Burley 21, Ky 17, male-sterile(MS) NC 107, TC 612, TC 613, Va 509 and Tl 1068. To estimated the modes of inheritances of DVT and cis-abienol known as the flavor and aroma compounds, the method of thin - layer chromatography (TLC) was used. It was considered that TLC procedure being relatively simple and reproducible in the selection of the breeding lines with higher DVT and cis-abienol. DVT was present in all of parents except MS NC 107, but cia-abienol presented only in Tl 1068. It was estimated from the TLC analysis of parents and Fl's that DVT and cis-abienol were each inherited by dominant gene or genes. But it was less clear to determine the presence or not of sucrose ester(SE) on the TLC plate. The eleven plants which had burley leaf color and also estimated to have DVT and cis-abienol were selected in the F2 population of three combinations.

• 한국연초학회지
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• 제15권2호
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• pp.145-151
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• 1993

Using a flue - cured tobacco variety, KF lH9, recently bred and released to farming, effect of growth regulators(fatty alcohol and C - MH) on the change of alkaloid contents and dynamics of MH uptake and distribution were investigated. An efficient sucker control method was also pursued. Treatment of fatty alcohol at button stage remarkably increased the rate of C - MH uptake. However, except in the plants treated with 2- fold volume of C - MH, residue of C -MH was kept under maximum residue level(80 ppd). C - MH residue in stalk was higher than in foliage in general. Compared to untreated control, leaf weight per unit area was increased by the treatment even in lower leaves where vigorous transfer of C - MH from treated tips was indicated and sucker control higher than 94.9% was achieved with all treatments except for sole half volume C - MH treatment at topping stage. Alkaloid content in foliage was reduced by dual treatment of fatty alcohol and C - MH but increased in stalk and root.

• 한국연초학회지
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• 제15권2호
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• pp.152-160
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• 1993

Using a flue- cured tobacco variety, KF 109, effect of growth regulators(fatty alcohol and C- MH) on the change of protein, DNA, and RNA were investigated. Generally, inhibition of DNA synthesis was observed soon after become notably reduced when checked on 14 days after the treatment. Fatty alcohol treatment appeared to alleviate the inhibition of DNA synthesis caused by the C - MH treatment. It was also observed that in the tips DNA content increased slightly at the early stage after the C - MH treatment but evident reduction of it was resulted from 7th day after the treatment. RNA content in cutters and tips was increased initially but variable transcription inhibitory activities - not so obvious as was observed in DNA synthesis - according to leaf positions were shown thereafter. Ripening of leaves probably due to senescence was advanced by the treatment of the growth regulators. DNA content in root was relatively higher in plants treated with the growth regulators while it was clearly decreased in stalk, However, RNA contents in tissue of stalk and root was not different with that of foliage. Increase of protein content in foliage as well as in stalk was evident 14 days after dual treatment of fatty alcohol and C - MH.

• 한국연초학회지
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• 제17권1호
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• pp.49-56
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• 1995

Morphology, chromosome and transmission of potato virus Y(PVY) of the green peach aphid populations collected from tobacco fields were examined. Based on the morphological characteristics, especially values of linear discriminant functions and length of ultimate rostral segment, 8 green peach aphid clones with different color morphs could be divided into two groups, namely Myzus persicae and M. nicotianae, according to the proposal by Blackman in 1987. The red (RED)-, Brown (BRN)- and green-colored (GR1, GR2) aphid clones belonged to the M. nicotianae type, while the pale green (PG1, PG2, PG3) and dark brown (DBR) clones to the M. persicae type. The karyotype of the pale green-colored clone (PG1, M. persicae type) appeared normal and was 2n=12 with no indication of chromosomal translocation. On the contrary, in the green-colored aphid clone (GR1, M. nicotianae type), translocation and dissociation of autosome 3 were often found, having karyotype of 2n=13. Both of the above aphid clones transmitted PVY-VN to tabacco plants (cv. Burley 21), but the GR1 clone had higher transmissibility than the PG1 clone.

• 원예과학기술지
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• 제17권4호
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• pp.473-475
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• 1999

본 연구는 antisense 유전자를 이용하여 웅성불임 식물체를 유기하는 웅성불임 유기 유전자의 발현을 억제함으로써 임성을 회복하기 위하여 실시하였다. 약특이 promoter(GBAN215-6 promoter)와 antisense 방향의 diphtheria toxin(DTx-A) 유전자로 제작된 pKDA215b로 담배(cv. petit Havana SR1)를 형질전환시키고 형질전환이 확인된 76개의 $R_0$ 세대를 자가수분하여 $R_1$ 세대를 획득하였다. $R_1$ 세대의 유전 분석후 antisene 유전자가 복수로 존재하는 5개의 $R_1$ 계통 (21505, 21507, 21511, 21522, 21525)을 선발하고 동형접합체를 획득하기 위해 자가수분을 하였다. 임성회복을 유도하고 회복친을 선발하기 위하여 antisense 유전자를 가진 $R_2$ 계통과 웅성불임 식물체를 교배하였다. 그 결과 꽃가루가 완전이 회복된 개체, 부분적으로 회복된 개체, 회복되지 않은 개체 등 3종류의 식물체를 획득하였으며 이들 식물체의 화분 이외의 표현형은 정상식물체와 같았고 그 기작 구명을 위해 선발하고 계속 연구가 진행되고 있다.

• 한국연초학회지
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• 제27권1호
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• pp.5-10
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• 2005

Studies on effect of Decyl alcohol EC and Butralin EC on tobacco sucker control were conducted in the tobacco field of Agro-tech Research Group of KT&G Central Research Institute, in 2004. Control effects of two chemicals were highest at the sucker length of 1$1\~2cm$. This indicates that the first application of Decyl alcohol should be conducted before button stage in the flue-cured and at the button stage in the burley tobacco. Effects of Decyl alcohol and Butralin on sucker control were $89.6\%\;and\;90.7\%$, respectively at 14 days after application in the variety KF118. But that of sequential application of two chemicals was $81.9\%$ at 30 days after application in the variety KF118 and $86.1\%$ in the variety KB303. Effect of two applications of Yellow ribbon or Butralin was highest in two varieties. The central downward spray showed a little higher control effect than the pouring of two chemicals. There was not significant difference of sucker control effect between forty and fifty times solution of Decyl alcohol in the variety KB303.

• 한국연초학회지
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• 제13권1호
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• pp.69-73
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• 1991

Varietal response to cool temperature(18$^{\circ}C$) and short (8hr.)-day treatment were investigated under controlled conditions of Phytotron in the Suwon Agronomy Experiment Station. Five flue-cured and one burley tobacco varieties (Nicotiana tabacum L.) were used, and the length of treatment ranging from 8 to 32 days at 3-day intervals and also includes continuous treatment up to the plants were flowered. The days to flower decreased significantly than the no treatment when the length of treatment was 11 to 14 days for the varieties NC82, Br.21 and NC22NF, and 17 to 26 days for SPG-28, Mc.944 and TC499, respectively. And also the number of leaves decreased significantly than the no treatment when the length of treatment was 8 days for NC22NF, 11days for NC82 and Br.21, 14 days for Mc.944, and 17 days for SPG-28 and TC499, respectively. The maximum decreasing ratio of the leaf number by the cool temperature and short-day treatment were 47.7 to 58.5% for NC82, Br.21 and NC22NF, and 38.9% for Mc.944, 33.4% for SPG-28 and 29.0% for TC499, respectively.

• 한국연초학회지
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• 제2권1호
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• pp.37-52
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• 1980

신개간지 토양에 대한 인산시용 수준별 석회, 퇴비증시, 퇴비와 붕소의 혼합시용에 따른 비옥도 증진의 효과가 연초의 수량 및 품질에 미치는 영향을 2년간에 걸쳐 시험한 결과를요약하면 다음과 같다. 1. 개량제 처리의 효과는 1차년도에서 보다 2차년도에 담배 생육과 엽면적지수와 단위엽 면적중을 월등하게 증가시켰다. 3. 개량제의 시용으로 초년도의 pH, 치환성염기등이 숙전과 같은 수준으로 개선되었으며, 처리별 비옥도 증진효과는 퇴비증시구가 컸다. 3. 2차년도 시험결과에서 수량은 대조구에 비하여 15∼25% 증수되었고, 품질은 처리간에 큰 차 없으나, 개량제 시용으로 대조구에 비하여 3 - 7% 향상되었다. 수익 (10a당 수납대금)은 인산흡수계수에 대한 3 %조절량+퇴비증시구가 가장 높아 대조구보다 34%의 증가를 가져왔다.