• 한국연초학회지
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• 제25권1호
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• pp.7-11
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• 2003

Repellencies of herb plants and essential oils against alate Myzus persicae were investigated in a insect cage, a screen house, and a tobacco field. Numbers of nymphs deposited by alate or apterous Myzur persicae were lower on rosemary(Rosmarinus officinalis), silver thyme (Thymus vulgaris), and lemon balm(Melissa of fcianazis). Rosemary and silver thyme show little repellency against alate Myzus persicae on a nearby tobacco plant Rosemary oil, peppermint (Mentha piperita) oil, and ginger oil showed repellency against alate Myzus persicae, but spearmint (Mentha spicata) oil and lavender oil showed little repellency in a screen house. Rosemary oil, ginger oil, peppermint oil, lavender oil, and spearmint oil showed repellency against alate Myzus persicae in a tobacco field, but there was no significant difference among oils.

• 한국연초학회지
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• 제8권2호
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• pp.43-49
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• 1986

Field experiment was conducted to investigate the effect of vinyl mulch on phosphate availability of soil and fertilizer in 1982. Growth of tobacco was more vigorous at each level of phosphate treatment in vinyl mulched than in bare soil. There was significant culture x Phosphorus treatment interaction for the yields of cured tobacco leaves. These results indicated that vinyl mulch was effective to improve phosphate availability of soil and fertilizer. Increase in soil temperature and maintenance of bulk density suitable for Phosphorus diffusion by vinyl mulch were likely to Play major roles on Phosphate availability, but other unknown factors appeared to be involved.

• Journal of Plant Biology
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• 제29권1호
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• pp.1-10
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• 1986

Intra- and inter-specific protoplast fusion of tobacco (Nicotiana tabacum cv. Virginia 115) and pea (Pisum sativum cv. Sparkle) were carried out in highly inhomogeneous alternating electric fields. Under the electric field of alternating current (AC, sine wave), 600 V/cm and 800 kHz for tobacco protoplast, and 600 V/cm and 700 kHz for pea protoplasts, the protoplasts were aggregated in pearl chains. Intra-specific protoplast fusions were most effectively induced within the aggregates of tobacco and pea, respectively, by the additional application of a single high field pulse of direct current (DC, square wave) at 1 kV/cm for 50 $mutextrm{s}$. Inter-specific fusions between protoplasts of the two plants were most effectively induced in the electric field of 600 V/cm and 700 kHz, and square wave pulse at 1 kV/cm for 50 $mutextrm{s}$. The duration of the pulse over the electrical breakdown voltages was simulated from 1 to 100 $mutextrm{s}$ in both tobacco and pea protoplast. The yield of the electrofusion products was significantly high (above 60%), compared with that (20%) of the standard fusion method by polyethylene glycol (PEG) 4,000, and the viability of electrofused protoplasts was above 70%, but that of PEG-fused protoplasts 8~16%, when determined by Evan's blue staining method.

• 한국연초학회지
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• 제24권1호
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• pp.7-12
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• 2002

During the screening or antiviral substances having inhibitory effect on tobacco mosaic virus(TMV) infection to tobacco plants, we found that a bacterial isolate, KTB61, which was identified as a Pseudomonas sp., strongly inhibited the formation of TMV local lesions. When the culture filtrate from KTB61 was applied on the upper surface of leaves of N. tabaccum Xanthi-nc tobacco at the same time of or 24 hours before TMV inoculation, almost complete inhibition was achieved. Incidence of systemic TMV infection to the susceptible tobacco cultivar, NC82, was reduced by 95% when TMV was inoculated onto the upper surface of leaves 24 hours after spraying the culture filtrate. Also 75∼80% of inhibitory effect was obtained by the inoculation of TMV onto the under surface of the leaves treated with culture filtrate 24 hours beforehand. In field trials, the TMV infection was reduced by 96.5% when the tobacco seedlings, N. tabaccum cv. NC82, were soaked with culture filtrate before transplanting.

• 한국연초학회지
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• 제6권2호
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• pp.179-184
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• 1984

This experiment was carried out to select the suitable burley varieties for paddy field. Two varieties, two $F_1$ hybrids and three $F_1$ hybrids with pale yellow line which were all early maturing were tested in the paddy field condition. The results were as follows. 1. Va 528 and all $F_1$ hybrids flowered 4-8 days earlier than Burley 21. 2. Leaf maturity among varieties and $F_1$ hybrids were not significantly different at first priming, but significant differences were detected from second to forth primings. (MS Ky 12x JB 7802-30)Fl and (MS Br 21 x L8)$F_1$ hybrids matured 3-5 days earlier than Burley 21. Pale yellow hyorids matured early, yielded high and were favorable in physical properties, but were low in quality. 4. Va 528 and (MS Br 21 x L8) $F_1$ hybrid showed earthy maturing, high yield and good quality compared with Burley 21. So two varieties were considered suitable for paddy field cultivation.

• 한국연초학회지
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• 제21권1호
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• pp.36-48
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• 1999

Field test was conducted in Chungbuk province to evaluate the soil fertility using landscape and soil attributes by application of geographic information system(GIS) in 48 tobacco fields during 2 years(1996 ; 23 fields, 1997 ; 25 fields). The soil fertility factors and fertilizer effects were estimated by twenty five independent variables including 13 chemical properties and 12 GIS databases. Twenty five independent variables were classified by two groups, 15 quantitative indexes and 10 qualitative indexes and were analyzed by multiple linear regression (MLR) of SAS, REG and GLM models. The estimation model for evaluation of soil fertility and fertilizer effect was made by giving the estimate coefficient for each quantitative index and for each group of qualitative index significantly selected by MLR. Estimation for soil fertility factors and fertilizer effects by independent variables was better by MLR than single regression showing gradually improvement by adding chemical properties, quantitative indexes and qualitative indexes of GIS. Consequently, it is assumed that this approach by MLR with quantitative and qualitative indexes was available as an evaluation model of soil fertility and recommendation of optimum fertilization for tobacco field.

• 한국연초학회지
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• 제6권2호
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• pp.155-168
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• 1984

Field test were conducted to select the best method for soil phosphorus test and to obtain the critical levels of each method for P fertilizer recommendations based on tobacco production. Truog method was recognized to be the mort suitable one for soil P test. N. Carolina, Bray No.1 and Olsen methods were followed in order. Bray No. 2 and Lancaster methods showed the least satisfaction. The critical levels of available P by lancaster, Bray No. 1, N. Carolina,01sen, and Truog methods were 55ppm, 32ppm, 21ppm, 19ppm, and 22ppm, respectively. Below the critical level a large yield response can be expected with 10kg $P_2O_5$/10a, above critical level little or no response is expected. In soils the more the initial available p, the more available P was carried over, while the more the total amount of silt and clay, the less the residual P was found. The increase in residual P by 10kg of $P_2O_5$ was greater than that of 5kg/10a. The chemical composition and the price of the cured leaf were not affected by the $P_2O_5$ application, indicating that the increase in the rate of $P_2O_5$ application would not exert on the quality of tobacco.

• 식물병연구
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• 제8권2호
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• pp.78-83
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• 2002

Tobacco diseases have not been recorded until 1900s in Korea, where tobacco plants were introduced at early 1700s. Practical researches on the disease have been conducted since mid 1960s. Major ten tobacco diseases were mosaic caused by tobacco mosaic virus·potato virus Y·cucumber mosaic virus, bacterial wilt, hollow stalk, wild fire caused by angular leaf spot strain, black shank, brown spot, powdery mildew and fusarium wilt. But their annual occurrences were varied according to changes of tobacco varieties and their cultivating practices. As no useful chemicals, several biological tactics have been developed to control the viral or bacterial diseases that give significant economic damages on sustainable crop yield, but not practicable to field farming condition yet. Transgenic tobacco plants containing foreign disease resistant genes have been developed by current bio-technology, but not released to farmers yet. Though some disease-resistant tobacco varieties have been developed by the conventional breeding technology and currently used by farmers, their disease controlling efficacy have been diminished by occurrence of the new strain or race. Future research on tobacco diseases has been focused on technical development to produce high quality tobacco with less production cost, which leads Korean tobacco industry to keep its competence against foreign industry and decreasing overall market.

• 한국연초학회지
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• 제13권2호
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• pp.42-47
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• 1991

Control effect of an avirulent bacterlocin-producing strain(ABPS) Y6l-1 of Pseudomonas solanacearum on bacterial wilt was 58.8 % when the bacterial suspension had poured onto the rhizosphere soil of tobacco cultivar NC82 on one day before transplanting to the field and of hilling time. Until eight weeks after inoculation, survival of the strain on rhizoplane and in stem of the plants inoculated was better than that of other four strains tested. It suggested that survival of the ABPS in and on the plants should be supported for the sufficient protection.

• 한국연초학회지
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• 제8권2호
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• pp.29-41
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• 1986

Effects of some mineral nutrients on the chlorine absorption by the plants, on the chemical constituents and physical properties of leaves were investigated tinder the paddy field and pot conditions. The chlorine content of cured leaf grown in paddy field was high in iron and manganese application groups and highest in combined application of iron and manganese. Lime application inhibited the absorption of chlorine and increased the yield and quality of cured leaf, and inhibited the absorption of iron and manganese those causing the grey leaf. Lime application reduced the leaf chlorine content and rate of muddy grey leaf by increasing the soil pH in acid soil.