• 보건교육건강증진학회지
• /
• 제7권2호
• /
• pp.71-77
• /
• 1990

Streptococcus mutans 10449 was cultured in sucrose-containing BHI broth with ethyl alcohol of different concentration from 1% to 18%, The pH of culture media was from pH 7.00 to pH 5.00. Tobacco smoke and tobacco extract were also used. Ethyl acohol began to inhibit sucrose fermentation by S. mutans at 2% and completely inhibited it between 9% and 18%. The lower the pH of media was, the stronger the inhibition of ethyl alcohol became. 9% Ethyl alcohol completely inhibited sucrose fermentation by S. mutans below pH 5.50, Inhibition by tobacco extract was obvious, but it did not inhibit the growth of S. mutans also. Therefore, the increase of caries activity in drinkers and smokers could be the result of indirect effect of alcohol and tobacco by oral ecology, behavior, or systematic course, rather than the result of direct effect of alcohol and tobacco to plaque bacteria and their metabolism.

• 한국연초학회지
• /
• 제11권2호
• /
• pp.143-148
• /
• 1989

Field experiment was conducted to find out the chemical characteristics of 12 Korean local varities, Local varities were compared with oriental, flue-cured and burley tobacco. Among tested varities, Hyangcho and Matskawa were desirable in respect of flavor, leaf color and texture. Hyangcho and Matskawa were higher in the content of nicotine and petroleum ether extract. Gajacho, Byeolcho and Kukjuo were higher in the content of reducing sugar, but lower nicotine, total nitrogen and petroleum ether extract. The nicotine and total nitrogen content of Kwangcho, Daruma and Catterton were lower than that of Br. 21. In the content of volatile organic acid which were major characteristic component of oriental tobacco, the Korean local varities were lower in the content of 3-methyl butanoic and 3-methyl pentanoic acid, but hirities, Hyangcho, Matskawa and Daruma were higher in the content of volatile organic acid. Korean local varities, especially small leaf group, were higher in the content of benzyl alcohol and furfural than any other type tobacco.

• Archives of Pharmacal Research
• /
• 제5권1호
• /
• pp.25-26
• /
• 1982

Nicotelline was directly analyzed from the crude tobacco extract by metastable studies. The metastable transitions of nicotelline were investigated by MS with DADI/MIKE spectrometry.

• 한국환경보건학회지
• /
• 제8권2호
• /
• pp.33-46
• /
• 1982

This experiment was carried out for the purpose of reducing alkaloid in reconstituted tobacco sheet and effluent of reconstituted tobacco sheet manufacturing company by treating oxidizing agents such as ozone, sodium hypochlorite, perchloric acid and hydrogen peroxide to tobacco extract created from the manufacturing process of reconstituted tobacco sheet. The effect of alkaloid reduction in tobacco extract by the volume added, time of treatment and pH of oxidizing agents were as follows: 1. When the solid rate of tobacco extract stood at 10 percent, the content of alkaloid, total sugar, total nitrogen and chlorine was 1,600mg/l, 11,000mg/l, 3,200mg/l and 4,000mg/l, respectively. 2. The effect of alkaloid reduction through ozone treatment was in proportion to time of ozone treatment. Alkaloid showed a 31.2 percent reduction under 8 hours' ozone treatment and 0.23g ozone consumed to remove lmg alkaloid. 3. Alkaloid reduction through sodium hypochlorite treatment was influenced by quantity of chlorine in sodium hypochlorite solution. To remove lmg alkaloid, 36.3mg chlorine was used. Reduction of alkaloid was not affected by time of sodium hypochlorite treatment, while showed the best reaction under pH 5-7. 4. The effect of alkaloid reduction by perchloric acid was under the control of the volume added and time of treatment of perchloric acid. The volume of perchloric acid required to remove alkaloid was on the decrease as time of treatment was getting longer. lmg alkaloid was removed by 0.15g perchloric acid under 8 hours' perchloric acid treatment. 5. Alkaloid reduction reacted slowly to the volume added and time of treatment of hydrogen peroxide. Under 8 hours' hydrogen peroxide treatment, it showed maximum removal, registering 10 percent alkaloid reduction.

• 고려인삼학회:학술대회논문집
• /
• 고려인삼학회 1997년도 춘계총회 및 학술대회
• /
• pp.15-15
• /
• 1997

• 생약학회지
• /
• 제23권1호
• /
• pp.24-28
• /
• 1992

Red ginseng extracts were prepared with various concentrations of ethanol. Extract yields were examined and saponins in the extracts were identified and determined by TLC and HPLC, respectively. Yields of the extracts, $19.7{\sim]50.3%$, were the highest in water extract and showed significant decrease with the increase of ethanol concentration used for extraction. Contrary to the extract yields, saponin yields from red ginseng were conspicuously increased with the increase of ethanol concentration and were $3.47{\sim}5.13%$ of crude saponins and $1.28{\sim}1.93%$ of six major ginsenosides. Saponin contents in the red ginseng extracts were $6.9{\sim}24.2%$, of crude saponin and $2.57{\sim}9.22%$, of six major ginsenosides.

• 한국연초학회지
• /
• 제4권1호
• /
• pp.73-78
• /
• 1982

A procedure for the simultaneous determination of reducing sugar, nicotine alkaloid, total sugar and ammonia has been developed. The four components of tobacco extracted from ground tobacco sample with 2 % acetic acid were deter mined with a Technicon autoanalyzer system. The relative errors in burley and flue-cured tobacco leaves were 3.6% in comparison with each official methods.

• 한국연초학회지
• /
• 제6권2호
• /
• pp.215-219
• /
• 1984

This experiment was conducted to investigate the effect of physical and chemical characteristics of lower grade tobacco with treatment of ozone. In ozonization of low grade tobacco, optimum moisture was 20 percentage, and decrease ratio of content of tobacco leaf was 30 percentage in nicotine , 15.9 percentage in total volatile base, 10.5 percentage in petroleum-ether-extract, 29.5 percentage in solanesol, and decrease ratio of cigarette smoke composition was 31 percentage in nicotine, 6.08 percentage in tar.

• 한국연초학회지
• /
• 제29권2호
• /
• pp.85-89
• /
• 2007

This study was conducted to evaluate the prediction of sensory characteristics of smoke from the leaf chemical compounds and characterize leaf chemical components for the best tobacco taste's leaves in oriental tobacco. For analytical and sensory evaluations, seventy two grades were used. Sensory evaluation of tobacco smoke for six attributes were scored on fifteen-point scale by $10{\sim}14$ expert panels trained to estimate smoking quality quantitatively. The major leaf chemical compounds to predict the sensory characteristics of smoke were ether extract for tobacco-like, nicotine for impact and total nitrogen/nicotine ratio for irritation, and total sugar for off taste & odor. Within ${\pm}20%$ range of difference, the predictable probabilities of sensory characteristics of smoke from the leaf chemical compounds were 87.5 % for off taste & odor and $94.4{\sim}98.6\;%$ for tobacco-like, impact and irritation. As a result of K-means cluster analysis on the basis of tobacco taste, the desirable leaf chemical compound contents were $5.9{\sim}8.3\;%$ in ether extract, $1.35{\sim}2.27\;%$ in nicotine and $1.17{\sim}2.24$ in total nitrogen/nicotine ratio. This study suggest that the some regression equations may be useful to predict the sensory characteristics of tobacco smoke with a few selected leaf chemical compounds in oriental tobacco and to select the oriental tobacco leaves by means of enhancing the tobacco taste of cigarette.

• 한국연초학회지
• /
• 제10권2호
• /
• pp.109-116
• /
• 1988

Oriental tobacco (KA 101) was transplanted from Mar. 25 to May 5 with 10 days interval in 1984 and 1985, and its agronomic characteristics, chemical properties ware compared to determine the Proper transplanting time of aromatic tobacco In Korea. As the transplanting was delayed, days to flowering of plant was shortened, length and width of largest leaf, leaf area index tended to decrease. Yield was highest for the Apr. 5th transplanting followed by Mar. 25th planting, then decreased as the planting date was delayed. Quality by price decreased as the transplanting was delayed later than Apr. 25. Delaying transplanting increased nicotine, total nitrogen and ash content, but decreased reducing sugar and petroleum ether extract of cured leaves, The content of volatile organic acids such as 3-methyl pentanoic acid was lower when it was transplanted later than Apr. 25th. Neophytadiene content increased as the transplanting was delayed, but there were no trends with the content of alcohols, aldehydes esters and ketones. Several quality indices including the ratio between the content of volatile organic acids plus petroleum ether extract and ash content plus pH was higher for the Apr. 5th transplanting.