• Clinical Endoscopy
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• v.57 no.3
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• pp.384-392
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• 2024

Background/Aims: Endoscopic ultrasound-guided tissue acquisition (EUS-TA) is a standard diagnostic method for biliary tract cancer (BTC), and samples obtained in this manner may be used for comprehensive genomic profiling (CGP). This study evaluated the utility of EUS-TA for CGP in a clinical setting and determined the factors associated with the adequacy of CGP in patients with BTC. Methods: CGP was attempted for 105 samples from 94 patients with BTC at the Aichi Cancer Center, Japan, from October 2019 to April 2022. Results: Overall, 77.1% (81/105) of the samples were adequate for CGP. For 22-G or 19-G fine-needle biopsy (FNB), the sample adequacy was 85.7% (36/42), which was similar to that of surgical specimens (94%, p=0.45). Univariate analysis revealed that 22-G or larger FNB needle usage (86%, p=0.003), the target primary lesions (88%, p=0.015), a target size ≥30 mm (100%, p=0.0013), and number of punctures (90%, p=0.016) were significantly positively associated with CGP sample adequacy. Conclusions: EUS-TA is useful for CGP tissue sampling in patients with BTC. In particular, the use of 22-G or larger FNB needles may allow for specimen adequacy comparable to that of surgical specimens.

• Analytical Science and Technology
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• v.27 no.6
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• pp.321-332
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• 2014

The accumulation levels of hexabromocyclododecanes (HBCDs) were measured in samples of muscle tissue from crucian carp (Carassius auratus) and also in samples of associated sediments. Samples were collected from 15 sites along the courses of four major rivers. Widespread contamination by HBCDs in this area was identified from the results of this investigation. HBCDs were found above the detection limit from all of the sampling sites. The ${\Sigma}HBCDs$ (sum of ${\alpha}$-, ${\beta}$-, and ${\gamma}$-HBCDs) levels ranged from 1.3 to 19 ng/g (lipid wt) in the crucian carp musscle tissue samples, and from 0.17 to 30 ng/g (dry wt) in the associated sediment samples. The predominance of ${\alpha}$-diastereomer in the muscle tissue of the carp and ${\gamma}$-diastereomer in the sediments was identified, accounting for 88% and 60% of the ${\Sigma}HBCDs$, respectively. The ${\Sigma}HBCDs$ levels in the muscle tissue samples of carp collected from the upstream sections of each river were much lower than those in the samples from carp in the downstream sections. In addition, the ${\Sigma}HBCDs$ levels in carp tissue samples from an isolated site were much lower than those in carp collected from the sites along the major rivers. This suggests that HBCD contamination in the rivers is likely to be sourced from the environment adjacent to them.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1128-1128
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• 2001

We have developed a probe for measuring the light levels inside illuminated fruit. The probe has minimal effect on the light levels being measured and enables the sampling of the light flux at any point within the fruit. We present experimental light extinction rates within apple, nashi, kiwifruit, and mandarin fruit. Moving from the illuminated side to the far side of the fruit, the extinction level follows an initial power law decay as the light diffuses into the fruit then reduces to an exponential decay through the rest of the fruit. Significant variations in the rates of light extinction are found in the core, skin and differing flesh regions. Monte Carlo simulations of the light distribution in fruit, which use scattering and absorption coefficients for the diffusely scattering tissue, and boundary conditions for the skin effects, produce results that follow the experimental results closely.

• Journal of Yeungnam Medical Science
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• v.3 no.1
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• pp.343-349
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• 1986

Histopathological diagnosis of brain stem glioma should be performed for the purpose of the determination of its management and clinical course, but its surgical biopsy has been followed by high mortality and morbidity. We performed the tissue sampling for histological examination with BRW stereotaxic system under local anesthesia successfully.

• Journal of Biomedical Engineering Research
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• v.21 no.2
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• pp.213-218
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• 2000

Pressure distributions of the soft tissue are valuable for understanding and diagnosing the disease characteristics due to the mechanical loading. Our system measures dynamic pressure distributions in real-time under the general PC environment, and analyzes various foot disorders. Main features of the developed system are as follows: (1) With the resistive pressure sensor matrix of 40${\times}$40 cells, the data is sent to the PC with the maximum sampling rate of 40 frames/sec. (2) For each frame, contact area, pressure and force are analyzed by graphic forms. Thus, various biomechanical parameters are easily determined at specific areas of interests. (3) A certain stance phase can be chosen for the analysis from the continuous walking, and the detailed biomechanical analysis can be done according to an arbitrary line dividing anterior/posterior or medial/lateral plantar areas. (4) The center of pressure (COP) is calculated and traced from the pressure distribution data, and thus the movement of the COP is monitored in detail. A few experiments revealed that our system successfully measured the dynamic plantar distribution during normal walking.

• Korean Journal of Radiology
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• v.23 no.6
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• pp.625-637
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• 2022

With regard to the indolent clinical characteristics of prostate cancer (PCa), the more selective detection of clinically significant PCa (CSC) has been emphasized in its diagnosis and management. Magnetic resonance imaging (MRI) has advanced technically, and recent international cooperation has provided a standardized imaging and reporting system for prostate MRI. Accordingly, prostate MRI has recently been investigated and utilized as a triage tool before biopsy to guide tissue sampling to increase the detection rate of CSC beyond the staging tool for patients in whom PCa was already confirmed on conventional systematic biopsy. Radiologists must understand the current paradigm shift for better PCa diagnosis and management. This article reviewed the recent literature, demonstrating the diagnostic value of pre-biopsy prostate MRI with targeted biopsy and discussed unsolved issues regarding the paradigm shift in the diagnosis of PCa.

• Korean Journal of Agricultural and Forest Meteorology
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• v.9 no.3
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• pp.203-208
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• 2007

The woody tissue respiration rates in stems ($R_{stem}$) of red pine (Pinus densiflora Sieb. et Zucc.) forest in Higashi-Hiroshima, west Japan, were measured using an open flow measurement system with several chambers in two seasons (the winter and summer in 2002). $R_{stem}$ ranged from 0.25 to $0.55{\mu}mol\;m^{-2}s^{-1}$ in winter, and from 1.25 to $1.63{\mu}mol\;m^{-2}s^{-1}$ in summer. The variability of $R_{stem}$ among the sampled trees (n=15) was similar between the two seasons, with the coefficient variation of about 23%. The numbers of sampling points required to estimate the stem respiration rate within 10 to 20% of its actual value were 24 and 6, respectively in both seasons (probability level is 95%). Based on the relationship between stem temperature and average $R_{stem}$ the $Q_{10}$ values of the winter and summer seasons were 1.49 and 1.45, respectively. The $R_{20}$($R_{stem}$ at $20^{\circ}C$ of stem temperature) was higher in summer ($1.23{\mu}mol\;m^{-2}s^{-1}$) than winter ($0.61{\mu}mol\;m^{-2}s^{-1}$). The woody tissue respiration in stems of red pine trees during the summer season amounted about 50% of the total respiration rates.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.2
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• pp.159-165
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• 1993

To clarify the effects of sink demand for assimilate on leaf photosynthetic rate, tissue composition, and leaf senescence of soybean [Glycine max (L.) Merr.] J plants, pod and leaf tissues were removed at growth stage $R^3$. Plant responses were measured every 10days from 2 through 42days following treatment. Leaves of depodded plants exhibited increased starch and chlorophyll contents and specific leaf weight. Stomatal resistance was also increased and leaf photosynthetic rate was reduced. Dry weight of vegetative tissues except leaves was increased by pod removal. Leaf removal resulted in a decreased starch content of leaves from 22 to 42days after treatment and that of roots at all sampling times. Specific leaf weight was decreased while leaf photosynthetic rate was increased. Stomatal resistance and chlorophyll content were little affected. Weight per seed was decreased 3.0% by leaf removal. Except for the seed, tissue protein contents were increased by pod removal but decreased by leaf removal, however, seed protein content was not affected by either. Apparent senescence was delayed by depodding. Both apparent and functional senescence were accelerated by leaf removal.

• Clinical Endoscopy
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• v.56 no.1
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• pp.107-113
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• 2023

Background/Aims: Immune checkpoint blockade has recently been reported to be effective in treating microsatellite instability (MSI)-high tumors. Therefore, sufficient sampling of histological specimens is necessary in cases of unresectable pancreatic cancer (UR-PC). This multicenter study investigated the efficacy of endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) using a Franseen needle for MSI evaluation in patients with UR-PC. Methods: A total of 89 patients with UR-PC who underwent endoscopic ultrasound-guided fine-needle aspiration (EUS-FNA) or EUS-FNB using 22-G needles at three hospitals in Japan (2018-2021) were enrolled. Fifty-six of these patients (FNB 23 and FNA 33) were followed up or evaluated for MSI. Patient characteristics, UR-PC data, and procedural outcomes were compared between patients who underwent EUS-FNB and those who underwent EUS-FNA. Results: No significant difference in terms of sufficient tissue acquisition for histology was observed between patients who underwent EUS-FNB and those who underwent EUS-FNA. MSI evaluation was possible significantly more with tissue samples obtained using EUS-FNB than with tissue samples obtained using EUS-FNA (82.6% [19/23] vs. 45.5% [15/33], respectively; p<0.01). In the multivariate analysis, EUS-FNB was the only significant factor influencing the possibility of MSI evaluation. Conclusions: EUS-FNB using a Franseen needle is desirable for ensuring sufficient tissue acquisition for MSI evaluation.

• Journal of Genetic Medicine
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• v.2 no.1
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• pp.23-26
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• 1998

Facioscapulohumeral muscular dystrophy (FSHD) is an autosomal dominant neuromuscular disorder which has been clinically shown to cause progressive weakness and result in atrophy of the facial muscles, shoulder girdle and upper arm muscles. The responsible gene for the FSHD has been located on chromosome 4q35-qter. The probes p13E-11 and pFR-1 detect DNA rearrangements associated with FSHD as under 28 kb DNA fragment in genomic southern analysis digested with EcoRI and the fragment contains 3.3 kb Kpn I tandem repeats. In this study, 4 fetuses with a family history of FSHD were analysed by genomic southern hybridization analysis with probes to determine whether they carried the deleted region. Of the 4 fetuses, three of them had mothers who were FSHD patients and the other one had a father affected with FSHD. After 10-11 weeks of gestation, we performed chorionic villi sampling and extracted DNA from uncultured and cultured tissue cells for the direct DNA analysis. The result of the southern analysis showed two fetuses having received about 15-18 kb of deleted genes from the father and the mother respectively, and found to be FSHD patients. The other two fetuses were shown to have two normal alleles from the parents and found to be normal. Two pregnancies which were determined to be normal were carried to term delivering two healthy babies.