• Journal of Animal Science and Technology
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• v.63 no.1
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• pp.114-124
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• 2021

The objective of this study was to characterize the enzymatic hydrolysis of lipopolysaccharide (LPS) by wheat phytase and to investigate the effects of wheat phytase-treated LPS on in vitro toxicity, cell viability and release of a pro-inflammatory cytokine, interleukin (IL)-8 by target cells compared with the intact LPS. The phosphatase activity of wheat phytase towards LPS was investigated in the presence or absence of inhibitors such as L-phenylalanine and L-homoarginine. In vitro toxicity of LPS hydrolyzed with wheat phytase in comparison to intact LPS was assessed. Cell viability in human aortic endothelial (HAE) cells exposed to LPS treated with wheat phytase in comparison to intact LPS was measured. The release of IL-8 in human intestinal epithelial cell line, HT-29 cells applied to LPS treated with wheat phytase in comparison to intact LPS was assayed. Wheat phytase hydrolyzed LPS, resulting in a significant release of inorganic phosphate for 1 h (p < 0.05). Furthermore, the degradation of LPS by wheat phytase was nearly unaffected by the addition of L-phenylalanine, the inhibitor of tissue-specific alkaline phosphatase or L-homoarginine, the inhibitor of tissue-non-specific alkaline phosphatase. Wheat phytase effectively reduced the in vitro toxicity of LPS, resulting in a retention of 63% and 54% of its initial toxicity after 1-3 h of the enzyme reaction, respectively (p < 0.05). Intact LPS decreased the cell viability of HAE cells. However, LPS dephosphorylated by wheat phytase counteracted the inhibitory effect on cell viability. LPS treated with wheat phytase decreased IL-8 secretion from intestinal epithelial cell line, HT-29 cell to 14% (p < 0.05) when compared with intact LPS. In conclusion, wheat phytase is a potential therapeutic candidate and prophylactic agent for control of infections induced by pathogenic Gram-negative bacteria and associated LPS-mediated inflammatory diseases in animal husbandry.

• The Journal of Korean Academy of Prosthodontics
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• v.53 no.3
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• pp.207-214
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• 2015

Due to the limitations of conventional removable partial denture prostheses to treat a cleft lip & palate patient who shows scar tissue on upper lip, excessive absorption of the maxillary residual alveolar ridge, and class III malocclusion with narrow palate and undergrowth of the maxilla, 4 implants were placed on the maxillary edentulous region and a maxillary removable implant-supported partial denture was planned using a CAD/CAM milled titanium bar. Unlike metal or gold casting technique which has shrinkage after the molding, CAD/CAM milled titanium bar is highly-precise, economical and lightweight. In practice, however, it is very hard to obtain accurate friction-fit from the milled bar and reduction in retention can occur due to repetitive insertion and removal of the denture. Various auxiliary retention systems (e.g. $ERA^{(R)}$, $CEKA^{(R)}$, magnetics, $Locator^{(R)}$ attachment), in order to deal with these problems, can be used to obtain additional retention, cost-effectiveness and ease of replacement. Out of diverse auxiliary attachments, $Locator^{(R)}$ has characteristics that are dual retentive, minimal in vertical height and convenient of attachment replacement. Drill and tapping method is simple and the replacement of the metal female part of $Locator^{(R)}$ attachment is convenient. In this case, the $Locator^{(R)}$ attachment is connected to the milled titanium bar fabricated by CAD/CAM, using the drill and tapping technique. Afterward, screw holes were formed and 3 $Locator^{(R)}$ attachments were secured with 20 Ncm holding force for additional retention. Following this procedure, satisfactory results were obtained in terms of aesthetic facial form, masticatory function and denture retention, and I hereby report this case.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.6
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• pp.885-893
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• 2001

This review summarizes some recent research into ways of improving the productivity of ruminal fermentation by increasing protein flow from the rumen and decreasing the breakdown of protein that results from the action of ruminal microorganisms. Proteinases derived from the plant seem to be of importance to the overall process of proteolysis in grazing animals. Thus, altering the expression of proteinases in grasses may be a way of improving their nutritive value for ruminants. Inhibiting rumen microbial activity in ammonia formation remains an important objective: new ways of inhibiting peptide and amino acid breakdown are described. Rumen protozoa cause much of the bacterial protein turnover which occurs in the rumen. The major impact of defaunation on N recycling in the sheep rumen is described. Alternatively, if the efficiency of microbial protein synthesis can be increased by judicious addition of certain individual amino acids, protein flow from ruminal fermentation may be increased. Proline may be a key amino acid for non-cellulolytic bacteria, while phenylalanine is important for cellulolytic species. Inhibiting rumen wall tissue breakdown appears to be an important mechanism by which the antibiotic, flavomycin, improves N retention in ruminants. A role for Fusobacterium necrophorum seems likely, and alternative methods for its regulation are required, since growth-promoting antibiotics will soon be banned in many countries.

• Journal of Korean Society for Atmospheric Environment
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• v.12 no.3
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• pp.307-314
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• 1996

The rate of evolution of ethylent by tomato plants was rapidly increased by ozone fumigation. In the present study, the mechanism of ethylent evolution by ozone was investigated in experiments with aminoethoxyvinylglycine (AVG) and tiron, which inhibit the formation of ethylene and peroxidation of lipids, respectively. Pretreatment with AVG significantly inhibited the ozone-induced ethylent evolution, but the treatment of plants with tiron did not inhibit. These results indicate that the induction of the evolution of ethylene by ozone involves the pathway via aminocyclopropane-1-carboxylate (ACC), while not released as a result of the peroxidation of lipids. Ozone-induced ethylent evolution was greater in dar- than light-incubated, intact tomato plants. The difference between dark- and light-ethylene evolution was examined with diuron, an inhibitor of photosynthetic electron transport. The inhibitor treatment promoted ethylent evolution. These results suggest that ethylent retention and metabolism in plants were regulated by internal $CO_2$ levels which, in turn, were controlled in large part by photosynthesis. Thus, ethylene was retained in illuminated leaf tissue under low intenal $CO_2$ concentration which may develop in a sealed container without exogenously supplied $CO_2$.

• The Journal of Advanced Prosthodontics
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• v.8 no.5
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• pp.411-416
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• 2016

Patients who underwent resection of maxilla due to benign or malignant tumor, or accident will have defect in palatal area. They get retention, support and stability from remaining tissues which are hardly optimal. The advantage of swing-lock attachment design is having multiple contacts on labial and lingual side of the abutment teeth by retentive strut and palatal bracing component. Because the force is distributed equally to abutment teeth, abutment teeth of poor prognosis can be benefited from it. It is also more advantageous to cover soft tissue defects which are hard to reach with conventional prosthesis. A 56-year-old female patient who had undergone a maxillectomy due to malignant melanoma complaining of loose and unstable surgical obturator. Surveyed crowns were placed on #12, 26, and 27. Teeth #11, 21, 22, and 23 had lingual rest seat and #24 had mesial rest seat to improve stability and support of the obturator. This clinical report presents the prosthetic management of a patient treated with obturator on the maxilla using swing-lock attachment to the remaining teeth.

• The Journal of the Korean dental association
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• v.54 no.7
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• pp.513-520
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• 2016

In crossed occlusion, displacement of removable partial denture is likely to occur during function due to different characteristics of abutment and supporting tissue. It increases discomfort to the patient. In addition, adverse effect on the residual ridge and abutment can induce an unfavorable prognosis of the denture. In this case, a small number of implants can be placed in strategic locations. Attachment can be added for additional support and retention of removable partial denture assisted by implants. This article describes the rehabilitation of a crossed occlusion patient using implant-assisted removable partial denture with Locator$^{(R)}$ attachment. After 24 months, the patient was satisfied with the aesthetic and function of the prosthesis that is maintained stable.

• The Journal of Advanced Prosthodontics
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• v.7 no.5
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• pp.380-385
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• 2015

PURPOSE. The aim of this study was to determine the frequency of significant panoramic radiographic findings and eventual treatment requirements before conventional or implant supported prosthetic treatment in asymptomatic edentulous patients. MATERIALS AND METHODS. A total of 743 asymptomatic edentulous patients were retrospectively evaluated using a digital panoramic system. We analyzed the radiographic findings, including impacted teeth, retained root fragments, foreign bodies, severe atrophy of the posterior maxillary alveolar bone, mucous retention cysts, soft tissue calcifications and radiopaque-radiolucent conditions. RESULTS. Four-hundred-eighty-seven (65.6%) patients had no radiographic finding. A total of 331 radiographic findings were detected in 256 (34%) patients. In 52.9% (n=175) of these conditions, surgical treatment was required before application of implant-supported fixed prosthesis. However, before application of conventional removable prosthesis surgical treatment was required for 6% (n=20) of these conditions. CONCLUSION. The edentulous patients who will have implant placement for implant-supported fixed prosthesis can frequently require additional surgical procedures to eliminate pathological conditions.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.35 no.3
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• pp.184-188
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• 2013

Post-traumatic enophthalmos is a relatively common problem following orbitozygomatic fractures. Bony-volume expansion and soft tissue atrophy are considered the main etiological causes of this condition. Enophthalmos is corrected mostly through reducing the enlarged orbit volume. Autogenous graft and various alloplastic materials are used for this purpose. Porous polyethylene is highly biocompatible, durable, and remarkably stable. Also, the titanium plate embedded in a porous polyethylene sheet provides radiographic visibility and increased sheet strength and contour retention. We present experiences of titanium reinforced porous polyethylene for correction of the traumatic enophthalmos with literature review.

• Journal of Food Hygiene and Safety
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• v.6 no.3
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• pp.165-169
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• 1991

The effect of gamma irradiation on physico-chemical properties of salted sea mustard (Undaria pinnatifida) was investigated. Chlorophyll and carotenoid pigment of salted sea mustard were partially decreased by irradiation. However there was no significant difference in the retention rate of pigment between control and 2 kGy-irradiated samples after six months of storage at around $10^{\circ}C$, ranging values of 74 to 77% in chlorophyll and 54 to 56% in carotenoid. Total organic acids and volatile acids associated with the organoleptic quality of sea mustard increased in the samples of lower salt concentrations and of higher storage temperatures. The softening of sample tissue by irradiation was shown to be correlated with the extraction properties of alginic acid.

• Journal of Acupuncture Research
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• v.35 no.4
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• pp.238-243
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• 2018

Background: Various factors can alter the efficacy of acupuncture treatment, such as the location of points, manipulations, depth of insertion, needle retention time, and needle type. In this study, the effect of needle diameter on the efficacy of acupuncture treatment was quantitatively evaluated. Methods: Five acupuncture needles of different diameters used in clinical practice were compared. Force on the porcine tissue phantom was measured using a sensor. Lifting-thrusting and twisting-rotating movements were performed using a needle insertion-measurement system. After repeated measurements, force magnitude was calculated and compared. Following this, we correlated needle diameter and force magnitude during lifting-thrusting and twisting-rotating movements. Results: The force magnitude was significantly altered between needle diameters during lifting-thrusting movements, as shown by a significant positive correlation between needle diameter and force magnitude. In contrast, there was no difference in force magnitude with different needle diameters during twisting-rotating movements. Conclusion: Needle diameter can significantly affect stimuli and force magnitude dependent upon the type of manipulation. Research into the effect of other needle type characteristics and stimulation method is necessary to fully elucidate the role of acupuncture needle choice in treatment efficacy.