• Title/Summary/Keyword: tissue retention

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Quantitative Comparison of Acupuncture Needle Force Generation According to Diameter

  • Lee, YeonSun;Bong, SungMin;Kim, Eun Jung;Lee, Seung Deok;Jung, Chan Yung
    • Journal of Acupuncture Research
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    • v.35 no.4
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    • pp.238-243
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    • 2018
  • Background: Various factors can alter the efficacy of acupuncture treatment, such as the location of points, manipulations, depth of insertion, needle retention time, and needle type. In this study, the effect of needle diameter on the efficacy of acupuncture treatment was quantitatively evaluated. Methods: Five acupuncture needles of different diameters used in clinical practice were compared. Force on the porcine tissue phantom was measured using a sensor. Lifting-thrusting and twisting-rotating movements were performed using a needle insertion-measurement system. After repeated measurements, force magnitude was calculated and compared. Following this, we correlated needle diameter and force magnitude during lifting-thrusting and twisting-rotating movements. Results: The force magnitude was significantly altered between needle diameters during lifting-thrusting movements, as shown by a significant positive correlation between needle diameter and force magnitude. In contrast, there was no difference in force magnitude with different needle diameters during twisting-rotating movements. Conclusion: Needle diameter can significantly affect stimuli and force magnitude dependent upon the type of manipulation. Research into the effect of other needle type characteristics and stimulation method is necessary to fully elucidate the role of acupuncture needle choice in treatment efficacy.

Freeze-dried bovine amniotic membrane as a cell delivery scaffold in a porcine model of radiation-induced chronic wounds

  • Oh, Daemyung;Son, Daegu;Kim, Jinhee;Kwon, Sun-Young
    • Archives of Plastic Surgery
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    • v.48 no.4
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    • pp.448-456
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    • 2021
  • Background Locoregional stem cell delivery is very important for increasing the efficiency of cell therapy. Amnisite BA (Amnisite) is a freeze-dried amniotic membrane harvested from bovine placenta. The objective of this study was to investigate the retention of cells of the stromal vascular fraction (SVF) on Amnisite and to determine the effects of cell-loaded Amnisite in a porcine radiation-induced chronic wound model. Methods Initially, experiments were conducted to find the most suitable hydration and incubation conditions for the attachment of SVF cells extracted from pig fat to Amnisite. Before seeding, SVFs were labeled with PKH67. The SVF cell-loaded Amnisite (group S), Amnisite only (group A), and polyurethane foam (group C) were applied to treat radiation-induced chronic wounds in a porcine model. Biopsy was performed at 10, 14, and 21 days post-operation for histological analysis. Results Retaining the SVF on Amnisite required 30 minutes for hydration and 1 hour for incubation. A PKH67 fluorescence study showed that Amnisite successfully delivered the SVF to the wounds. In histological analysis, group S showed increased re-epithelialization and revascularization with decreased inflammation at 10 days post-operation. Conclusions SVFs had acceptable adherence on hydrated Amnisite, with successful cell delivery to a radiation-induced chronic wound model.

Mucocele in the maxillary sinus involving the orbit: A report of 2 cases

  • Yeom, Han-Gyeol;Lee, Wan;Han, Su-Il;Lee, Jae-Hoon;Lee, Byung-Do
    • Imaging Science in Dentistry
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    • v.52 no.3
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    • pp.327-332
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    • 2022
  • Mucocele of the paranasal sinuses is a benign, slow-growing, expansile lesion. Maxillary sinus mucoceles are usually associated with painless bulging of the cheek; however, orbital expansion is rarely observed. Maxillary sinus mucoceles can be classified as primary or secondary according to their etiology. An impediment to sinus ostium ventilation is thought to be the cause of primary mucocele, while sequestering of residual mucosa after surgery in the wound and long-term retention of tissue fluid have been suggested to lead to the formation of secondary mucocele. This report presents 2 cases of primary and secondary mucoceles, with a focus on radiographic features. As primary and superiorly positioned secondary maxillary sinus mucoceles are uncommon and their close proximity to the orbit predisposes the patient to significant morbidity, the authors expect that this report will contribute to a better understanding and diagnosis of maxillary sinus mucocele involving the orbit.

The Usefulness of Integrated PET/CT to Distinguish between Benignancy and Malignancy in Solitary Pulmonary Nodule (고립성 폐결절의 악성 감별에서 Integrated PET/CT의 유용성)

  • Park, Won-Jong;Kim, Dong-Hee;Yu, Sung-Ken;Shin, Kyeong-Cheol;Chung, Jin-Hong;Lee, Kwan-Ho;Chun, Kyung-Ah;Cho, Ihn-Ho
    • Journal of Yeungnam Medical Science
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    • v.23 no.2
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    • pp.205-212
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    • 2006
  • Background: Malignant pulmonary nodules account for about 30 to 40 percent of solitary pulmonary nodules (SPN). Therefore, tissue characterization of SPNs is very important. Recently, PET/CT has been widely used for tissue characterization, and has become of importance. The purpose of this study was to compare and to assess multiple factors in PET/CT comparing benign and malignant nodules. Materials and Method: Nineteen patients with SPN underwent PET/CT and biopsy. The difference of standardized uptake value 1 (SUV1), standardized uptake value 2 (SUV2) and retention index in PET/CT between malignancy and benignancy were compared by Levene's test. Result: There were twelve malignant and seven benign nodules. SUV1 and SUV2 were significantly different between malignant nodule and benign nodule (p=0.006 and 0.022), but retention index was not significantly different between malignant nodule and benign nodule (p=0.526). By receiver-operating-characteristic (ROC) analysis, the sensitivity was 66.7% and the specificity was 71.4% at a cut off value of 5.40 in SUV1. The sensitivity was 75% and the specificity was 71.4% at cut off value of 7.45 in SUV2. Conclusion: There was a statistically significant difference in SUV1 and SUV2 between benign and malignant nodules. However, the cut off value of SUV1 and SUV2 by receiver-operating-characteristic (ROC) analysis was 5.40 and 7.45 which is different from previous studies. Therefore, studies on a larger sample of patients are required for confirmation.

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Biodisc Regeneration Using Annulus Fibrosus Cell with Hyaluronic Acid Impregnated Small Intestinal Submucosa Sponge (히알루론산이 함유된 SIS 스폰지와 섬유륜세포를 이용한 디스크재생)

  • Hong, Hee-Kyung;Lee, Seon-Kyoung;Song, Yi-Seul;Kim, Dae-Sung;Eom, Shin;Kim, Hyoung-Eun;Lee, Dong-Won;Khang, Gil-Son
    • Polymer(Korea)
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    • v.34 no.3
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    • pp.282-288
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    • 2010
  • The porcine small intestinal submucosa (SIS) has been widely used as a biomaterial without immuno rejection responses and hyalunonic acid (HA) can be used as biocompatible materials to regenerate tissue. We developed the SIS sponge and HA loaded SIS sponges (SIS/HA) for the possibility of the application of the tissue engineering using annulus fibrosus (AF). SEM observation shows that SIS and SIS/HA sponges have interconnected and open pores. We demonstrated the presence of HA in SIS/HA sponge from C-O functional group observed by the FTIR analysis. In conclusion, we suggest that SIS/HA sponge may be useful to tissue engineering using AF cell. This may be due to the enhanced biocompatibility and higher water retention capacity of HA.

A HISTOLOGIC STUDY OF INITIAL CHANCE AND REPAIR OF TOOTH AND PERIODONTAL TISSUE IN EXTRUSION OF YOUNG ADULT DOGS (유성견 소구치 정출시 치아 및 치주 조직의 초기 조직학적 변화 및 재생에 관한 연구)

  • Yoon, Byung-sun;Cha, Kyung-Suk;Lee, Jin-Woo
    • The korean journal of orthodontics
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    • v.28 no.3 s.68
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    • pp.419-429
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    • 1998
  • This study was carried out in order to study early histologic changes and repair reaction appling to extrusive force for 3rd premolar of adult dogs. After 1 week of extrusive force with elastic chain, one of dogs was sacrified and after 3 weeks retention period, another dog was sacrified. The paraffin sections of samples were stained with Hematoxylin - Eosin and Masson's Trichrome and were examed by light microscopy . The obtained results as follows 1. In Hematoxylin - Eosin and Masson Trichrome stain of control group , the periodontal ligament width was constant from apical third to cervical third of the root and periodontal fiber arrangement was horizontal or oblique in cervical third. oblique in middle third, oblique in apical third of root. in alveolar bone, smooth appearance was shown 2. In Group 1, all periodontal fiber arrangement was oblique toward tooth, and the periodontal ligament width increased Partially PDL was ruptured in apex. In MT stain, immature bone formation was seen at alveolar crest area. Active bone formation was observed along the one side of alveolus, and apical portion of pulp was involved with blood vessel rupture , vacuolization of pulp tissue and hyperemia 3. In Group 2, most periodontal ligament arrangement and PDL width was repaired and fiber density increased. In MT stain, mineralization of immature bone on the alveolar crest was progressed. In pulp, vacuole and hyperemia was diminished and fibrotic change was diminished 4. After 3 week periodontal ligament has more repair ability than pulp tissue. pulp was involved with vacuolization and fibrosis, so it takes more time for repair.

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Long-term Management of a Gingival Fibromatosis Patient with the Primary Dentition (유치열기에서 나타난 치은섬유종증 환자의 장기간 관리)

  • Kang, Chungmin;Lee, Jaeho;Choi, Hyungjun;Song, Jeseon;Kim, Seongoh
    • Journal of the korean academy of Pediatric Dentistry
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    • v.41 no.4
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    • pp.328-334
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    • 2014
  • Gingival fibromatosis is a rare oral condition that is characterized by proliferative fibrous overgrowth of the attached gingiva, the marginal gingiva, and the interdental papilla, typically presenting in the growth period. A case of a 27-month-old girl with a generalized severe gingival overgrowth is described herein. The patient had no known systemic disease, but enlarged gingival tissue had gradually covered her teeth. The excess gingival tissue was removed by conventional gingivectomy, which involved extraction of the retentive primary teeth under general anesthesia when she was 5 years old. Post surgical follow-up at 18 months after the surgery demonstrated no recurrence. Resectional surgery of the enlarged gingival tissue is the treatment choice for gingival fibromatosis, although there is a high risk of recurrence. More frequent professional follow-ups and oral hygiene instruction might be required. A delay in the surgical treatment may have significant consequences for the patient, such as primary dentition retention and consequent delay in the eruption of the permanent teeth, difficulties in mastication and phonation, malpositioning of the teeth, and psychological problems. Early surgical treatment should be performed according to the severity of enlargement.

MARSUPIALIZATION FOR TREATMENT OF ORAL RANULA (조대술에 의한 하마종의 치료)

  • Kang, Dong-Gyun;Hwang, Kyung-Mun;Kim, Eun-Jung;Kim, Hyun-Jung;Kim, Young-Jin;Nam, Soon-Hyeun
    • Journal of the korean academy of Pediatric Dentistry
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    • v.33 no.1
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    • pp.139-145
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    • 2006
  • Ranula is a mucocele which occurs at the mouth floor and is mostly related to sublingual gland. In other words ranula is definded as a pseudocyst which occurs as the secretion duct of sublingual gland is destructed there as the saliva from the secretion duct flows out and retention in the soft tissue. The cause of ranula is destruction or obstruction of the duct. The clinical findings of ranula is a painless, unilateral bluish transparent swelling around the frenum and shows fluctuation when palpated. Histological finding represent a formation of cavity inside the connective tissue, but a pseudo-cyst can be seen which the wall of the cyst is composed of granulation tissue rather than epithelial cells. The first treatment of ranula can be considered as marsupialization. which induces the inner wall of the Ranula to be a part of oral mucosa. This case report shows a treatment of marsupialization with gauze packing in a young patient representing a clinical finding of characteristic ranula.

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Effects of Glucomannan, Carrageenan, Carboxymethyl cellulose, and Transglutaminase-B on the Quality Properties of Pork Patties Containing Pork Skin Connective Tissue (Glucomannan, Carrageenan, Carboxymethyl cellulose 및 Transglutaminase-B 첨가가 돈육껍질결체조직 함유 돈육 패티의 품질 특성에 미치는 영향)

  • Jin, Sang-Keun;Yang, Han-Sul
    • Journal of Animal Science and Technology
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    • v.54 no.4
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    • pp.307-313
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    • 2012
  • This study was conducted to investigate the effects of glucomannan (GMN), carrageenan (CAR), carboxymethyl cellulose (CMC), and transglutaminase-B (TGB) on the quality and storage properties of pork patties manufactured with pork skin connective tissue during 21 d of storage at $4^{\circ}C$. Results showed that CIE color values like lightness, redness and yellowness did not differ significantly among the pork patties. Sensory attributes also did not differ between the treatments (P>0.05). However, cooking loss was significantly lower in the group with added GMN, CAR, CMC, and TGB compared to the control at 21 d of storage. The shear force value of GMN and TGB were lower than the control at 21 d of storage (P<0.05). The pork patties added with GMN and TGB had lower thiobarbituric acid reactive substances (TBARS) values than the control at 1 or 21 d of storage (P<0.05). Volatile basic nitrogen (VBN) values of all treatment samples was lower than the control at 21 d of storage (P<0.05). Therefore, result of cooking loss suggested that the decrease in shear force in GMN and TGB were due to higher moisture retention. Also, the pork skin connective tissue with added GMN and TGB decreased lipid oxidation of pork patties.

Influence of Various Root Media in Pot Growth of 'Seolhyang' Strawberry on the Growth of Daughter Plants and Early Yield after Transplant ('설향' 딸기 포트육묘를 위한 혼합상토 종류가 자묘의 생육과 정식 후 초기수량에 미치는 영향)

  • Park, Gab Soon;Kim, Yeoung Chil;Ann, Seoung Won;Kang, Hee Kyoung;Choi, Jong Myung
    • Horticultural Science & Technology
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    • v.33 no.2
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    • pp.219-226
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    • 2015
  • The objective of this research was to investigate the influence of various root media on the growth of mother and daughter plants during propagation and early yield after transplanting of 'Seolhyang' strawberry. To achieve this, daughter plants were fixed to connected small pots that contained expanded rice-hull (ERH), a strawberry-specialized commercial medium (SSCM), soil mother materials (SMM), or loamy sand (LS). Then, growth of daughter plants in above- and below-ground tissue as well as early yield after transplanting to plastic house soil were investigated. The growth of daughter plants in terms of plant height, leaf area and fresh weight were the highest in the SSCM treatment. Root growth in terms of the amount of primary roots and root dry weight were the highest in the treatments of ERH and SMM and the lowest in that of SSCM, among treatments tested. The ERH treatment also showed the highest values among treatments in root length, surface area and volume when roots with 0 to 0.4 mm in diameter were investigated. The flower bud differentiation of daughter plants began on Sept. 3 in the ERH treatments, earlier than the SMM (Sept. 5) and in SSCM (Sept. 7) treatments. The tissue N contents of daughter plants were in the range of 1.41 to 1.55% in all treatments, and no significant differences were observed among treatments. This indicates that the low moisture retention capacity of ERH and water stress, rather than tissue N contents, promote the flower differentiation of daughter plants. In the evaluation of early yield after transplant, the ERH treatment of showed the highest yield in the period from November to December, reaching 667 g fruit weight per 10 plants. The yields per 10 plants in the other treatments were 581 g in SMM, 475 g in SSCM and 295 g in LS. Above results imply that the various root media have different effects on the growth of daughter plants as well as flower bud differentiation. Therefore, improvement in early yield after transplant can be achieved through selection of proper root medium for daughter plant propagation.