• Pediatric Infection and Vaccine
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• v.23 no.1
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• pp.62-66
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• 2016

Skin and soft tissue infections (SSTIs) caused by community-associated (CA)-methicillin-resistant Staphylococcus aureus (MRSA) have become a worldwide concern. An otherwise healthy 16-month-old Korean girl was admitted because of skin abscess on the left chest wall with a history of recurrent SSTIs since the age of 6 months. Immunologic evaluation including serum immunoglobulin level and nitroblue-tetrazolium (NBT) test were normal. Pus and nasal swab cultures revealed CA-MRSA ST714-SCCmec type IV with the Panton-Valentine leukocidin (PVL) genes, which was initially reported in the Netherlands in 2006 and has not been previously reported in Korea. The skin abscesses were successfully treated by needle aspiration and the use of antibiotics. In addition, nasal mupirocin was applied as a decolonization method. No more episodes of SSTI were observed over a follow-up period of 10 months.

• Tissue Engineering and Regenerative Medicine
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• v.15 no.6
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• pp.751-760
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• 2018

BACKGROUND: Bone tissue engineering based on pluripotent stem cells (PSCs) is a new approach to deal with bone defects. Protocols have been developed to generate osteoblasts from PSCs. However, the low efficiency of this process is still an important issue that needs to be resolved. Many studies have aimed to improve efficiency, but developing accurate methods to determine efficacy is also critical. Studies using pluripotency to estimate efficacy are rare. Telomerase is highly associated with pluripotency. METHODS: We have described a quantitative method to measure telomerase activity, telomeric repeat elongation assay based on quartz crystal microbalance (QCM). To investigate whether this method could be used to determine the efficiency of in vitro osteogenic differentiation based on pluripotency, we measured the pluripotency pattern of cultures through stemness gene expression, proliferation ability and telomerase activity, measured by QCM. RESULTS: We showed that the pluripotency pattern determined by QCM was similar to the patterns of proliferation ability and gene expression, which showed a slight upregulation at the late stages, within the context of the general downregulation tendency during differentiation. Additionally, a comprehensive gene expression pattern covering nearly every stage of differentiation was identified. CONCLUSION: Therefore, this assay may be powerful tools for determining the efficiency of differentiation systems based on pluripotency. In this study, we not only introduce a new method for determining efficiency based on pluripotency, but also provide more information about the characteristics of osteogenic differentiation which help facilitate future development of more efficient protocols.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.34 no.2
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• pp.142-146
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• 1989

Anther and/or tissue culture of cross pollinated crops would be very important because it can result in the direct use of haploids or doubled haploids for developing superior hybrids or varieties. The objective of the study was to investigate the response frequencies in anther and/or tissue-cultured hybrids of corn. pearl millet and buckwheat to identify agronomically acceptable germplasm of the crops. 27 crosses of corn inbred lines were evaluated by plating their anthers on N6. MS and Yu-Pei media. Two genotypes of FR1l41/FR16 hybrid cultured on N6 medium and Fla 2BT73/S6013 hybrid cultured on N6 medium responded with one anther producing calli when plated after 5$^{\circ}C$ low temperature treatment for one week. Immature embryos of corn hybrid Suwon 19 responded producing calli that were regenerated to plants at a 8.6 percent success rate. Of the 20 corn hybrids. immature tessels of FR1l41/FR16. B68/A1l6N//KS15. KS16/KS17. GA209/DB578 and SDB126/GA209 crosses responded at a relatively higher success rate producing calli that were regenerated to plants. In tissue culture of elongating culms of pearl millet x Napier grass interspecific hybrid. 2.5-4.0mm long pieces of the culm were good for callus induction resulting in higher success rate. The epicotyl of buckwheat was very good for tissue culture. and the node produced the plants regenerated directly without callus induction on the B5 medium containing I ppm BA and 0.05 ppm IBA. There were great differences in response to anther and/or tissue culture of corn, pearl millet and buckwheat due to genotype x medium and environment interactions.

• Journal of Microbiology
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• v.45 no.2
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• pp.146-152
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• 2007

A mutant exhibiting decreased cytotoxic activity toward INT-407 intestinal epithelial cells and carrying a mutation in the rtx gene cluster that consists of rtxCA and rtxBDE operons was screened from a library of V. vulnificus mutants. The functions of the rtxA gene, assessed by constructing an isogenic mutant and evaluating its phenotypic changes, demonstrated that RtxA is essential for the virulence of V. vulnificus in mice as well as in tissue cultures.

• Advances in pediatric surgery
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• v.4 no.2
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• pp.144-147
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• 1998

The sacrococcygeal region is the frequent site for meningocele, congenital dermal sinus and pilonidal cyst. From May 1995 to July 1998, we have treated 8 neonatal patients with an abscess in the sacrococcygeal area. The mean age at onset was 8.3 days with a range from 6 to 11 days. The sex ratio was 5:3 with male preponderance. Mild fever was the only systemic symptom. Ultrasonogram revealed a slightly hypo echoic lesion in the subcutaneous tissue which became more hypoechoic with time. Pus cultures showed $Staph.$ $aureus$ in 7 patients, two of them had mixed infection with $E.$ $coli$ and other 2 had methicillin-resistant $Staph.$ $aureus.$ The remaining one patient had a mixed infection with $Klebsiella$ and $Proteus.$ Histopathological examination revealed non-specific granuloma and fibrinoid necrotic debris. All patients were easily treated by incision, drainage and proper antibiotics.

• Asian-Australasian Journal of Animal Sciences
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• v.4 no.3
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• pp.203-209
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• 1991

Collection, evaluation and preservation of tropical forage grasses, napier grass breeding, tissue culture of pangola grass and alfalfa selection were conducted in Taiwan. The results showed that some species such as pangola grass (Digitaria decumbens) with good performance and wide adaptability were selected and released. Forage yield and quality of napier grass have been improved and have good results in animal performance. Embryogenic callus cultures from young inflorescences and stem segments of pangola grass could provide an alternative method for rapid propagation and improvement. Three better varieties of alfalfa were selected. They maintain satisfactory stands for two or three years on well-drained sand loam and loam soil, and used only as annual crop in flat area and acid soil. However, more studies in forage breeding for acid soil arc required to maximise the forage quality and animal production. Thus, animal fed with forages of high quality and a few grains supplement will be the future achivement in livestock industry.

• KSBB Journal
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• v.13 no.5
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• pp.530-534
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• 1998

The addition of the ceramic powder as state of bare in culture medium has stimulated the growth of Achyranthes japonica in both the disorganized cell and the plantlet. The grwoth rate of Hyoscyamus niger adventitious root and Pylatycodon grandiflorum hairy root was enhanced up to 100 and 250%, respectively, even though Scopolia parviflora hairy root and Hyoscyamus albus adventitious root were not. The ceramic powder has enhanced the growth of H. niger adventitious root even in a test tube immersed into its culture medium to irradiate alone without any direct contact. The ceramic powder seems to have a significant role on both the growth and the physiological action of some plants.

• Journal of Plant Biotechnology
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• v.34 no.3
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• pp.197-200
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• 2007

Mature seeds of Artemisia annua L. were placed onto Murashige and Skoog's (MS) medium supplemented with $4.52\;{\mu}M$ 2,4-dichlorophenoxyacetic acid (2,4-D). After 6 weeks of culture, off-white, compact calluses were formed on the plumule of seedlings at a frequency of 5.9%. Calluses were subcultured on the same medium. After an additional 2 weeks of subculture, calluses produced a few somatic embryos at a frequency of 28.8%. Upon transfer to MS basal medium, calluses producing a few somatic embryos gave rise to numerous somatic embryos, which subsequently developed into plantlets. Plantlets were successfully transplanted to potting soil and grown to maturity in a greenhouse.

• BMB Reports
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• v.50 no.2
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• pp.79-84
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• 2017

Emphysema, a pathologic component of the chronic obstructive pulmonary disease, causes irreversible destruction of lung. Many researchers have reported that mesenchymal stem cells can regenerate lung tissue after emphysema. We evaluated if spheroid human adipose-derived mesenchymal stem cells (ASCs) showed greater regenerative effects than dissociated ASCs in mice with elastase-induced emphysema. ASCs were administered via an intrapleural route. Mice injected with spheroid ASCs showed improved regeneration of lung tissues, increased expression of growth factors such as fibroblast growth factor-2 (FGF2) and hepatocyte growth factor (HGF), and a reduction in proteases with an induction of protease inhibitors when compared with mice injected with dissociated ASCs. Our findings indicate that spheroid ASCs show better regeneration of lung tissues than dissociated ACSs in mice with elastase-induced emphysema.

• Environmental Analysis Health and Toxicology
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• v.16 no.2
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• pp.57-66
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• 2001

Marine organisms such as fish (Sebastes schlegeli) and mussels were cultured in sea water tanks placed at Dukpo area which was contaminated by the two oil spill accidents. Results showed that PAHs concentrations in flesh tissue were higher than in fish fiver. This was explained by the cytochrome P45O 1A induction in fish limier after PAHs exposure. Other studies showed that higher PAHs levels were detected in mussels cultures in oil contaminated area than in control site. From these results, we concluded that Dukpo area is still polluted by oil including PAHs and it takes a long time to recover of oil contamination after the oil spill accidents.