• Development and Reproduction
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• v.21 no.1
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• pp.47-54
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• 2017

Unlike mouse results, cloning efficiency of nuclear transfer from porcine induced pluripotent stem cells (piPSCs) is very low. The present study was performed to investigate the effect of cell cycle inhibitors on the cell cycle synchronization of piPSCs. piPSCs were generated using combination of six human transcriptional factors under stem cell culture condition. To examine the efficiency of cell cycle synchronization, piPSCs were cultured on a matrigel coated plate with stem cell media and they were treated with staurosporine (STA, 20 nM), daidzein (DAI, $100{\mu}M$), roscovitine (ROSC, $10{\mu}M$), or olomoucine (OLO, $200{\mu}M$) for 12 h. Flow Cytometry (FACs) data showed that piPSCs in control were in G1 ($37.5{\pm}0.2%$), S ($34.0{\pm}0.6%$) and G2/M ($28.5{\pm}0.4%$). The proportion of cells at G1 in DAI group was significantly higher than that in control, while STA, ROSC and OLO treatments could not block the cell cycle of piPSCs. Both of viability and apoptosis were affected by STA and ROSC treatment, but there were no significantly differences between control and DAI groups. Real-Time qPCR and FACs results revealed that DAI treatment did not affect the expression of pluripotent gene, Oct4. In case of OLO, it did not affect both of viability and apoptosis, but Oct4 expression was significantly decreased. Our results suggest that DAI could be used for synchronizing piPSCs at G1 stage and has any deleterious effect on survival and pluripotency sustaining of piPSCs.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2009.10a
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• pp.225-228
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• 2009

The simulated indoor pass-by noise measurement system is the tool to measure and evaluate the pass-by noise at the test laboratory, without doing measurement at the field. This measurement system can realize the precision measurement under the specific condition and overcome the limitations of the field measurement, i.e. weather conditions, repeatability, .. This measurement system is done in time domain process using the array techniques, which synchronizes the time signals. The reliability of the obtained result depends on the array shapes, which can generate the moving source effect. In this paper, the validations are checked focusing the time domain synchronization of the signals with the optimum microphone array shape.

• The Transactions of the Korean Institute of Electrical Engineers P
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• v.59 no.1
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• pp.47-52
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• 2010

The low power of ultra-wideband (UWB) signal makes the acquisition of UWB signal be a more challenging task. In this paper, we propose the method of high order template signal technique that reduces the synchronization time. Experimental results are presented to show the improvements of performance in the mean acquisition time (MAT) and the probability of detection. The performance compared with the serial search, the truly random search and the random permutation search. It is shown that over typical UWB multipath channels, a random permutation search scheme may yield lower MAT than serial search.

• Proceedings of the KIEE Conference
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• 2005.07d
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• pp.2489-2491
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• 2005

Currently, Programmable Logic Contorller(PLC) uses Real Time Operation System(RTOS) as basic OS. RTOS executes defined results as to defined time. General features of RTOS emphasize the priority in each task, high-speed process of external interrupt, task scheduling, synchronization in task, the limitation of memory capacity. For safety critical placement, PLC software needs Verification and Validation(V&V). For example, nuclear power plant. In this paper, PLC RTOS is verified by formal methods. Particularly, formal method V&V uses verification tool called 'STATEMATE', and shows the results.

• 제어로봇시스템학회:학술대회논문집
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• 1996.10b
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• pp.1312-1315
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• 1996

Polishing work for a curved surface die demands simple and repetitive operations and requires much time while it also demands high precision. Therefore it is operated by skilled worker in handiwork. But workers avoid polishing work gradually because of the poor environments such as dust and noise. In order to reduce the polishing time and to alleviate the problem of shortage of skilled workers, researches for automation of polishing have been pursued in the developed countries such as Japan. In this research we develop a polishing robot with 2 degrees of freedom motion and pneumatic system, and attach it to machining center with 3 degrees of freedom to form an automatic polishing system which keeps the polishing tool vertically on the surface of die and maintains constant pneumatic pressure. The developed polishing robot is controlled by real time sliding mode control using DSP(digital signal processor). A synchronization between machining center and polishing robot is accomplished by using M code of machining center. A performance experiment for polishing work is executed by the developed polishing robot.

• 제어로봇시스템학회:학술대회논문집
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• 2002.10a
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• pp.33.1-33
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• 2002

The INS provides high rate position, velocity and attitude data with good short-term stability while the GPS provides position and velocity data with long-term stability. By integrating the INS with GPS, a navigation system can be achieved to provide highly accurate navigation performance. For the best performance, time synchronization of GPS and INS data is very important in GPS/INS integrated system. But, it is impossible to synchronize them exactly due to the communication and computation time-delay. In this paper, to reduce the error caused by the measurement time-delay in GPS/INS integrated systems, error compensation methods using separate bias Kalman filter are suggested for both the...

• MALSORI
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• no.59
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• pp.69-87
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• 2006

Among the conventional time-scale modification (TSM) methods, the synchronized overlap and add (SOLA) method is widely used due to its good performance relative to computational complexity But the SOLA method remains complex due to its synchronization procedure using the normalized cross-correlation function. In this paper, we introduce a computationally efficient SOLA method utilizing 3 level center clipping method, as well as zero-crossing and level-crossing information. The result of subjective preference test indicates that the proposed method can reduce the computational complexity by over 80% compared with the conventional SOLA method without serious degradation of synthesized speech quality.

• Proceedings of the KIEE Conference
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• 2008.07a
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• pp.277-279
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• 2008

현재 많은 전력계통용 보호제어 벤더들은 변전소 자동화 국제표준 프로토콜인 IEC 61850을 적용한 전력 장비들을 개발하여 변전소에 적용하고 있다. 이러한 많은 장비들은 표준화된 IEC 61850 프로토콜을 적용하여 상호 호환성을 유지하였으며, 기존에 적용되고 있는 표준화된 기술들을 채택하고 있다. 변전소 장비들은 정밀한 시간 동기를 위해서 표준화된 시각 동기 프로토콜인 SNTP(Simple Network Time Protocol)나 NTP(Network Time Protocol)를 사용하고 있다. 본 연구에서는 IEC 61850 프로토콜을 적용한 IED가 시각 동기 장치로부터 시간 동기화되는 방안에 대해 연구하였다.

• Proceedings of the KSMRM Conference
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• 2003.10a
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• pp.44-44
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• 2003

To determine feasibility of contrast-enhanced MRI (co-MRI) at true end-diastole (ED) free from limitation of time for inversion-recovery and trigger window for quantifying transmural extent of infarction. 대상 및 방법： MRI was performed in 18 patients with myocardial infarction. Cine imaging and co-MRI with same registered slices in short axis were peformed. To allow true ED co-MRI, ECG synchronization should use two RR-intervals for one acquisition of a segment of k-space by setting the heart rate to half that of the true heart rate. Trigger delay time was adjusted to the RR-interval for imaging at ED and to the sum of RR-interval plus the time between R-wave and the end-systole (ES) determined in cine images for imaging at ES.

• Proceedings of the KSMRM Conference
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• 2003.10a
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• pp.91-91
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• 2003

To determine feasibility of contrast-enhanced MRI (co-MRI) at true end-diastole (ED) free from limitation of time for inversion-recovery and trigger window for quantifying transmural extent of infarction. 대상 및 방법： MRI was peformed in 18 patients with myocardial infarction. Cine imaging and co-MRI with same registered slices in short axis were performed. To allow true ED co-MRI, ECG synchronization should use two RR-intervals for one acquisition of a segment of k-space by setting the heart rate to half that of the true heart rate. Trigger delay time was adjusted to the RR-interval for imaging at ED and to the sum of RR-interval plus the time between R-wave and the end-systole (ES) determined in cine images for imaging at ES.