• 제목/요약/키워드: time-recording

검색결과 862건 처리시간 0.03초

배양조건에 의한 일과성 저산소상태 후 신경세포회복의 차이 (Difference of Neuronal Recovery by Incubation Condition after Transient Hypoxia)

  • 문수현;오재인;박윤관;정흥섭;이훈갑;이기찬
    • Journal of Korean Neurosurgical Society
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    • 제29권9호
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    • pp.1161-1170
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    • 2000
  • Objective : The transverse hippocampal slice is one of the most commonly studied in vitro models of mammalian brain physiology. However, despite its broad usage, there has been no standardization of slice preparation techniques or recording condition. It is well known that variations in recording conditions can result in profound different effects to neuronal responses. Evoked field potentials, recorded extracellularly, were used to investigate the effects of variations in hippocampal slice preparation protocol on hypoxia responses of CA1 neurones. Material & Methods : Before hypoxic injury, hippocampal slices were incubated for 4 hours. During incubation period, the slices were placed in a incubation chamber($21^{\circ}C$) for recovery from preparation injury and then transferred to recording chamber($34^{\circ}C$) for more recovery and baseline electric recording with current stimulation(0.1Hz). Various time periods in incubation chamber and recording chamber were applied to each experimental group(group 1=60min : 180min, group 2=90min : 150min, group 3=180min : 60min, time in incubation chamber : time in recording chamber) before 10 min hypoxia produced by replacing 95% $O_2$+5% $CO_2$ mixed gas to 95% $N_2$+5% $CO_2$ gas. Calcium, Magnesium ions and several drugs effecting on glutamate receptor also were studied. Recoveries from hypoxic injury of hippocampal slices were estimated by percent recovery of population spike(PS). Statistic analysis of study were performed using paired t-test. Results : The percent recovery of PS after 10min hypoxia was considerably enhanced by increasing the period of current stimulation during incubation period before hypoxic injury. Temperature effect on the result of this experiment was also studied(group 4) but the result from this showed no statistic significance. Low magnesium ion concentration of artificial CSF(Mg-free aCSF) during incubation period enhanced the recovery of PS but low calcium (calcium-free) and high magnesium ion concentration(2mM) reduced it after hypoxic injury. L-glutamate($100{\mu}M$) and AP-5($50{\mu}M$) had no effect on the recovery of PS but CNQX($10{\mu}M$) in artificial CSF during incubation period markedly enhanced the recovery of PS. Co-treatment of AP-5($50{\mu}M$), CNQX($10{\mu}M$) and high magnesium concentration(2mM) enhanced recovery of PS in immediate following period of hypoxic injury but the effect of cotreatment after then decayed rapidly and lost statistic significance. Conclusions : Judging from above results, the condition of baseline recording is important in observing the recovery of population spike after hypoxia, and the time and the condition should be controled more strictly to obtain reliable results.

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실시간 TEM 분석에 유용한 영상 기록 프로그램, VirtualDub (VirtualDub as a Useful Program for Video Recording in Real-time TEM Analysis)

  • 김진규;오상호;송경;유승조;김영민
    • Applied Microscopy
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    • 제40권1호
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    • pp.47-51
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    • 2010
  • The capability of real-time observation in TEM is quite useful to study dynamic phenomena of materials in a certain variable ambience. In performing the experiment, the choice of video recording program is an important factor to obtain high quality of movie streaming. Window Movie Maker (WMM) is generally recommended as a default video recording program if one uses "DV Capture" function in DigitalMicrograph$^{TM}$ (DM) software. However, the image quality does not often satisfy the condition for high-resolution microscopic analysis since the severe information loss in the final result occurs during the conversion process. As a good candidate to overcome this problem, Virtual-Dub is highly recommended since the information loss can be minimized through the streaming process. In this report, we demonstrated how useful VirtualDub works in a high-resolution movie recording. Quantitative comparison of the information quality between the images recorded by each software, WMM and VirtualDub, was carried out based on histogram analysis. As a result, the image recorded by VirtualDub was improved ~13% in brightness and ~122% in contrast compared with the image obtained by WMM at the same imaging condition. Remarkably, the gray gradation (meaning an amount of information) becomes wider up to ~115% than that of the WMM result.

Digital Recording Device를 ol용한 회전중인 블레이드 표면의 압력섭동 측정 (Measurement of Surface Pressure Fluctuations on a Rotating Blade Using a Digital Recording Device)

  • 윤정식;강웅;성형진
    • 대한기계학회논문집B
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    • 제29권10호
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    • pp.1119-1129
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    • 2005
  • A new measurement system of wall pressure fluctuations on a rotating machinery, composed of digital recording device, was developed and evaluated. The small-sized digital recording device was attached on the rotating machinery and then was detached for data reduction. In order to obtain the system transfer function of the digital recording system, a dynamic calibration was performed utilizing the signal from a 1/8 inch B&K microphone as input. The time history of the unsteady pressure was then reconstructed from the output of the sensor by using this transfer function. The reconstructed pressure signals showed good agreement with the reference signal in both temporal and spectral sense. This sensor was then used to measure the wall pressure fluctuations on a rotating blade. An array of microphones were installed on the blade in the circumferential and radial directions. Various statistical moments were obtained from the measurement data set. Comparison of these quantities with the existing studies demonstrated satisfactory agreement. These tests give credence to the relevance and reliability of this device for applications in more complicated turbulent rotating machineries.

고밀도 자기 기록 채널의 비선형 왜곡 추정 기법 (An estimation technique for nonlinear distortion in high-density magnetic recording channels)

  • 이남진;오대선;조용수;김기호
    • 한국통신학회논문지
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    • 제22권11호
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    • pp.2439-2450
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    • 1997
  • 디지털 자기 기록 채널의 기록 밀도가 증가하면 기록 채널의 비선형 왜곡이 발생하고, 이로 인해 PRML이나 DFE와 같은 디지털 검출 방식의 성능이 급격히 저하된다. 고밀도 기록을 저해하는 요소는 비선형 천이 이동과 부분 삭제로 분류되는데, 이 중 비선형 천이 이동은 사전 보상 기법에 의해 보상 가능하고, 부분 삭제 현상의 보상을 위해서는 정교한 비선형 동화 기법이 필요하다. 고밀도 기록을 달성하기 위해서는 이러한 비선형 왜곡과 관련된 파라메타의 정확한 추정이 매우 중요하다. 본 논문에서는 시간 영역에서 제안된 적응 알고리듬을 사용하여 위의 두 비선형 왜곡 영향을 분리할 수 있는 새로운 추정 기법을 논하고, 제안된 적응 방식으로 비선형 파라메타를 바이어스 없이 추정할 수 있음을 컴퓨터의 실험을 통해 확인하였다.

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광변색 회절 이미지 형성을 위한 트라이아진계 메타아크릴레이트 모노머를 포함한 광고분자 필름의 특성평가 (Characterization of Photopolymers films containing triazine methacrylate monomer for photochromic diffractive image formation)

  • 오현진;이지예;김은경
    • 정보저장시스템학회논문집
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    • 제3권4호
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    • pp.173-177
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    • 2007
  • A photo-reactive methacrylate monomer having triazine as a core component was examined for photochromic diffractive image formation. Photochromic photopolymer films as a recording media contained a monomer mixture of triazine difunctional metharylate (DT) and ethylene glycol phenyl ether acrylate, binder polymer, photo initiator, and a photochromic spiro-oxazine dye. The content of photochromic dye was changed to examine the effect of photochromophore on diffraction efficiency and real holographic image formation. Holographic recording was performed on the photopolymer film by the combination of reference and probe beam. The diffraction efficiency of the photopolymer film in real-time measurement reached a maximum of $\sim90%$ within 30s. It was highly dependent on the photochromophore contents. After holographic recording, the color of the recorded area was changed under UV light (365 nm) and reversibly bleached to original color upon exposure to a visible light source. Films containing only photochromophore without monomer mixture were not reactive under the recording beam (491nm). Diffractive image formation and mechanism of the holographic recording in the presence of photochromophore will be presented.

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Ginseng Extract Regulates the Alterations of Sleep Architecture and EEG Power Spectra in Restraint Stressed Rats

  • Ma, Yuan;Eun, Jae-Soon;Yang, Shulong;Lee, Kwang-Seung;Lee, Eun-Sil;Kim, Chung-Soo;Oh, Ki-Wan
    • Journal of Ginseng Research
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    • 제34권1호
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    • pp.30-40
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    • 2010
  • The present investigation was conducted to evaluate the regulation of sleep architecture by the red ginseng water extract (RGE) in acutely and chronically restraint stressed rats. Adult rats were fitted with sleep.wake recording electrodes. Following post-surgical recovery, rats were extensively habituated for freely moving polygraphic recording conditions. Polygraphic signs of sleep-wake activities were recorded for 24 h after RGE administration and induction of stress and were analyzed to understand the regulation of sleep architecture. Acute stress decreased wakefulness and increased total sleep, non-rapid eye movement (NREM) sleep, and rapid eye movement (REM) sleep in both the daytime and nighttime recording. RGE shortened the daytime NREM and REM sleep, without changing the wakefulness and total sleep. RGE increased nighttime wakefulness, and decreased total, NREM and REM sleep. Chronic stress increased wakefulness and decreased total sleep in the daytime recording, and increased REM and decreased NREM sleep in both the day and night time recording. RGE ameliorated chronic stress and induced alterations of REM and NREM sleep in the day and night time sleep architecture. Acute and chronic stress could also induce alternations in cortex electroencephalogram (EEG) recording during NREM, REM sleep and wakefulness. These findings suggest that RGE may modulate the sleep behavior in acutely and chronically stressed rats and the ameliorating effect of RGE on the sleep architecture may involve in modulation of $\alpha$-, $\theta$- and $\delta$- wave activities of the cortical EEG.

고속기록기의 제어운용 소프트웨어 분석 및 개발에 관한 연구 (A Study on Development and Analysis of Control Operation Software of High-Speed Recorder)

  • 황철준;오세진
    • 융합신호처리학회논문지
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    • 제10권4호
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    • pp.280-288
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    • 2009
  • 본 논문에서는 한국우주전파관측망(Korean VLBI Network; KVN)을 통하여 천체의 미약한 우주전파를 고속으로 기록하는 Mark5B 고속기록기 시스템의 제어운용 소프트웨어를 분석하고 VSI(VLBI Standard Interface) 형식의 시각정보를 기록할 수 있는 소프트웨어를 개발하였다. 고속기록기 시스템의 제어운용 소프트웨어는 대용량 하드디스크를 제어할 수 있는 RAID 보드를 구동하는 소프트웨어와 네트워크를 통한 제어운용을 할 수 있다. 핵심 제어 소프트웨어가 시스템에서 구동하고 있으면 네트워크로 입력된 명령을 해석하고 실행 후 결과를 다시 네트워크를 통해 출력하도록 구성되어 있다. 이러한 소프트웨어 분석을 통하여 기록된 관측데이터를 상관처리하기 위해 필요한 시각정보를 함께 기록할 수 있는 소프트웨어를 본 논문에서 개발하였다. 개발된 소프트웨어는 데이터를 기록할 때 입력되는 1PPS 신호의 인터럽트를 확인한 후 데이터 기록과 함께 기록시각정보도 함께 데이터 기록시간동안 기록한다. 개발한 소프트웨어의 정상동작을 확인하기 위해 실제 관측을 수행하였으며, 기록한 데이터의 분석을 통하여 그 유효성을 확인하였다.

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광디스크의 노화에 관한 주사 탐침 현미경 연구 (Scanning Probe Microscopy Study on the Degradation of Optical Recoding Disks by Environmental Factors)

  • 윤만영;신현창
    • 한국인쇄학회지
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    • 제29권3호
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    • pp.97-104
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    • 2011
  • The storing ability of information of optical disks directly depends on the physical property of recording unit cells. It means that the degradation of optical disks ultimately causes the loss of the physical and chemical properties of recording unit cells and leads also information, too. We investigated the degradation and life time of optical disks which tell us the longevity of the preservation of information. Optical disks were aged using the accelerated aging system and studied by optical reflectivity spectroscopy and atomic force microscopy(AFM), and the preservation environment of electronic media in National central library of Korea also were analysed. Results show that the double reflective coated optical disks have good preservation of recording information but revealed some deformation of dye area in the AFM images. It means that we should include the mechanical and chemical degradation of the optical disks in the life time expectation evaluation.

A 10-Lead Long Duration Ambulatory ECG Design -Minimizing power consumption-

  • Kim, Eung-Kyeu;Lee, Hoon-Kyeu
    • 융합신호처리학회논문지
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    • 제16권1호
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    • pp.29-34
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    • 2015
  • The ECG(Electrocardiograph) ambulatory test as called Holter is performed usually to diagnose several heart diseases causing different arrhythmias. This paper exposes the insights of the design of a 10-lead ambulatory ECG recorder. Reducing the size and minimizing the power consumption of the ECG recorder are crucial to allow long recording time without causing discomfort to the patient. This paper proposes lower hardware design and differential compression algorithm to extend the maximum 72 hours recording time in consideration of smaller and light-weighted recorder size. The performance results by newly introduced compression algorithm are shown and discussed.

Effect of temperature on the rheological properties of dental interocclusal recording materials

  • Pae, Ahran;Lee, Ho-Rim;Kim, Hyeong-Seob
    • Korea-Australia Rheology Journal
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    • 제20권4호
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    • pp.221-226
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    • 2008
  • The purpose of this study was to compare rheological properties of six dental interocclusal recording materials and to investigate the effect of temperature on the rheological properties during setting. Five polyvinylsiloxane materials and one polyether material were investigated in this study. The storage modulus (G') and the loss factor ($tan{\delta}$) were measured from 30s after mixing during setting, using the universal dynamic spectrometer. Viscoelastic properties were evaluated by means of G' and $tan{\delta}$ from 5 repeats at $21^{\circ}C$ and $33^{\circ}C$. Individual changes during setting were also evaluated. All data were statistically analyzed using one-way ANOVA and multiple comparison $Scheff{\acute{e}}$ test at the significance level of 0.05. The mean of G was checked at $t_{set}$ (the setting time provided from manufacturer) and $t_{300}$ (the end of experimental time) and the mean of $tan{\delta}$ was checked at to and $t_{set}$. Whereas the increase of the G' value showed generally exponential changes at $21^{\circ}C$, the change of the G' value at $33^{\circ}C$ displayed sigmoidal curves during setting. The change of loss factor $tan{\delta}$ during setting varied. Within the limitations of this study, dental interocclusal recording materials had different viscoelastic properties and most of the materials showed different fluidity at $21^{\circ}C$ and $33^{\circ}C$.