• The Korean Journal of Physiology and Pharmacology
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• v.2 no.4
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• pp.419-425
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• 1998

To clarify the effect of extracellular ATP in cultured C6 glioma cells, ATP-induced cytosolic free calcium ($[Ca^{2+}]_i$) mobilization and cell proliferation were investigated. ATP-induced $[Ca^{2+}]_i$ increased in a dose-dependent manner $(10^{-7}\;M{\sim}10^{-3}\;M)$. ATP-induced $[Ca^{2+}]_i$ increases were slightly slowed in extracellular calcium-free conditions especially in sustained phase. ATP-induced $[Ca^{2+}]_i$ increment was also inhibited by the pretreatment of U73122, a phospholipase C (PLC) inhibitor, in a time-dependent manner. Suramin, a putative $P_{2Y}$ receptor antagonist, dose-dependently weakened ATP-induced $[Ca^{2+}]_i$ mobilization. Significant increases in cell proliferation were observed at 2, 3, and 4 days after ATP was added. Stimulated cell proliferation was also observed with adenosine at days 2 and 3. This cell proliferation was significantly inhibited by the treatment with suramin. Ionomycin also stimulated cell proliferation in a concentration-dependent manner. In conclusion, we suggest that extracellular ATP stimulates C6 glioma cell proliferation via intracellular free calcium mobilization mediated by purinoceptor.

• Steel and Composite Structures
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• v.32 no.2
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• pp.239-252
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• 2019

Since conical sandwich shells are important structures in the modern industries, in this paper, for the first time, vibration behavior of the truncated conical sandwich shells which include temperature dependent porous FG face sheets and temperature dependent homogeneous core in various thermal conditions are investigated. A high order theory of sandwich shells which modified by considering the flexibility of the core and nonlinear von Karman strains are utilized. Power law rule which modified by considering the two types of porosity volume fractions are applied to model the functionally graded materials. By utilizing the Hamilton's energy principle, and considering the in-plane and thermal stresses in the face-sheets and the core, the governing equations are obtained. A Galerkin procedure is used to solve the equations in a simply supported boundary condition. Uniform, linear and nonlinear temperature distributions are used to model the effect of the temperature changing in the sandwich shell. To verify the results of this study, they are compared with FEM results obtained by Abaqus software and for special cases with the results in literatures. Eigen frequencies variations are surveyed versus the temperature changing, geometrical effects, porosity, and some others in the numerical examples.

• Biomedical Science Letters
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• v.26 no.4
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• pp.256-266
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• 2020

Carboxypeptidase D (CPD) is a zinc-dependent protease, which is highly expressed in macrophages, and is thought to participate in inflammatory processes. In the present study, we investigated the possible regulatory effect of all-trans retinoic acid (ATRA), which is an active form of vitamin A and plays a critical regulatory role in both the innate and adaptive immunity, on CPD expression and secretion in human monocytic THP-1 cells. CPD mRNA expression first increased, from a concentration as low as 10 nM ATRA to a maximum level of expression, at 1 μM. ATRA enhanced intracellular CPD expression in a time- and concentration-dependent manner but did not affect cell surface CPD expression. Interestingly, 9-cis-RA did not affect CPD expression. Additionally, an experiment with RAR/RXR selective agonist or antagonists demonstrated that ATRA-induced enhancement of CPD expression was RAR/RXR dependent. ATRA also enhanced CPD secretion from THP-1 cells; however, this enhancement was RAR/RXR-independent. The anti-inflammatory agent dexamethasone reversed ATRA-induced enhancement of CPD expression and secretion. Our results suggest ATRA exerts regulatory effects on expression and secretion of CPD in human monocytes, and ATRA-induced CPD secretion may be associated with inflammatory response.

• Asia-Pacific Journal of Business
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• v.11 no.2
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• pp.27-43
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• 2020

Purpose - This study explores the impact of the real estate industry on related industries for the perspective of Chinese steel companies. Design/methodology/approach - The impact of housing prices on the 41 listed steel companies' performance was analyzed by using the panel data model. We used two kinds of housing price indexes that are set in the panel data models to estimate the range of the real estate market, driving the performance growth of steel listed companies. Moreover, the net profit of steel companies is used as the dependent variable. To test the stability of the model, ROA used as a dependent variable for the robustness test. Also, to avoid the time trend of housing prices, this paper selects the growth rate of housing prices as the primary research variable. After Fisher-type testings, there is no unit root problem in both independent and dependent variables. Findings - The results indicated that the rise in the housing price has a positive influence on the steel company performance. When the housing price increases by 1%, the net profit of steel enterprises will increase by 5 to 20 million yuan. Research implications or Originality - In this paper, empirical data at the micro-level and panel model are used to quantify China's real estate industry's driving effect on the iron and steel industry, providing evidence from the microdata level. It helps us to understand further the status and role of China's real estate industry in the economic structure.

• Journal of The Korean Astronomical Society
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• v.38 no.2
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• pp.175-178
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• 2005

We show that next-generation galaxy surveys such as KAOS (the Kilo-Aperture Optical Spectro-graph)will constrain dark energy even if the baryon oscillations are missing from the monopole power spectrum and the bias is scale- and time-dependent KAOS will accurately measure the quadrupole power spectrum which gives the leading anisotropies in the power spectrum in redshift space due to peculiar velocities, the finger of God effect, as well as the Alcock-Paczynski effect. The combination of monopole and quadrupole power spectra powerfully breaks the degeneracy between the bias parameters and dark energy and, in the complete absence of baryon oscillations ($\Omega$b = 0), leads to a roughly $500\%$ improvement in constraints on dark energy compared with the monopole spectrum alone. As a result, for KAOS the worst case with no oscillations has dark energy errors only mildly degraded relative to the ideal case, providing insurance on the robustness of KAOS constraints on dark energy. We show that nonlinear effects are crucial in correctly evaluating the quadrupole and significantly improving the constraints on dark energy when we allow for multi-parameter scale-dependent bias.

• Korean Journal of Pharmacognosy
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• v.50 no.4
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• pp.277-284
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• 2019

Rumex crispus is known to have anticancer, antioxidant, antibacterial, and bone loss inhibitory activities. Mast cells are critical immune cells that induce a type 1 IgE-mediated allergic reaction. However, there are no reports of inhibitory effects of Rumex crispus on mast cells and allergic reactions. In this study, we performed some experiments to investigate whether Rumex crispus ethanol extract(RCE) has any inhibitory effect on antigen-induced type I allergic response in vitro and in vivo. RCE inhibited degranulation of IgE-mediated mast cells(IC₅₀, ~57 ㎍/ml) and cytokine production such as TNF-α and IL-4 in a dose-dependent manner. In vivo, RCE significantly inhibited passive cutaneous anaphylaxis(PCA)(ED₅₀, ~198 mg/kg) in mice. Furthermore, RCE inhibited degranulation of MCs in ear tissue of mice with PCA. Mechanism studies showed that RCE inhibited the activation of Syk and Syk-dependent pathway such as LAT, PLC-γ, Akt, and MAP Kinase. Our results demonstrate for the first time that RCE inhibits type I hypersensitive response by suppressing the activity of Syk in mast cells, thereby reducing degranulation and cytokine production. Taken together, RCE could be used as a novel therapeutic material to suppress allergic diseases.

• Journal of Physiology & Pathology in Korean Medicine
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• v.27 no.2
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• pp.239-245
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• 2013

In in vitro study, the anti-platelet aggregation effect of Shiitake mushroom extract was examined by measuring the collagen induced platelet aggregation and the DPPH radical scavenging. In in vitro study, anti-thrombotic effect of Shiitake mushroom extract was examined using the carotid artery thrombosis rat model. Carotid artery thrombosis rat model was made by 35% $FeCl_3$ treatment. After that, we investigate thrombus weight and blood flow. In platelet aggregation test, the extract significantly inhibited platelet aggregation in a concentration dependent manner(p<.001). Also, extract increased DPPH radical scavenging activity in a concentration dependent manner. Extract significantly inhibited thrombus weight to compare with control group. And blood passage time were shorter in the Shiitake mushroom extract supplemented groups than in control group. These results provide experimental evidence that Shiitake mushroom can be used to prevent platelet aggregation and thrombosis, then could apply the clinical diseases such as cardiovascular disease, and so on.

• Earthquakes and Structures
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• v.12 no.5
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• pp.529-541
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• 2017

According to the definition, progressive collapse could occur due to the initial partial failure of the structural members which by spreading to the adjacent members, could result in partial or overall collapse of the structure. Up to now, most researchers have investigated the progressive collapse due to explosion, fire or impact loads. But new research has shown that the seismic load could also be a factor for initiation of the progressive collapse. In this research, the progressive collapse capacity for the 5 and 15-story steel special moment resisting frames using push-down nonlinear static analysis, and nonlinear dynamic analysis under the gravity loads specified in the GSA Guidelines, were studied. After identifying the critical members, in order to investigate the seismic progressive collapse, the 5-story steel special moment resisting frame was analyzed by the nonlinear time history analysis under the effect of earthquakes with different characteristics. In order to account for the initial damage, one of the critical columns was weakened at the initiation of the earthquake or its Peak Ground Acceleration (PGA). The results of progressive collapse analyses showed that the potential of progressive collapse is considerably dependent upon location of the removed column and the number of stories, also the results of seismic progressive collapse showed that the dynamic response of column removal under the seismic load is completely dependent on earthquake characteristics like Arias intensity, PGA and earthquake frequency contents.

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.3
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• pp.1-12
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• 2006

Purpose : This study was aimed to investigate the inhibitory effect of Leonurus sibiricus on the proliferation of human uterine leiomyoma cells and the expression of gene related the mechanism of cell apoptosis. Methods : We counted the number of death cells treated with indicated concentration of Leonurus sibiricus and investigated cell death rate by MTS assay. Furthermore, flow cytometry analysis and DNA fragmentation assay were used to dissect between necrosis and apoptosis and then we observed the differential gene expression by western blot analysis. Results : Leonurus sibiricus significantly inhibited the proliferation of uterine leiomyoma cell in a dose-dependent and time dependent manner. Fluorescence activated cell sorter (FACS) analysis indicated that Leonurus sibiricus induced G1 cell cycle arrest. Leonurus sibiricus enhanced the expression of p27 and p53 with cell cycle arrest. Conclusion : These findings suggest that Leonurus sibiricus is a candidate agent for the treatment of uterine leiomyoma. p27, $p53^{1}$ may play an important role in Leonurus sibiricus-induced cell cycle arrest and cell growth inhibition.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.4 no.1
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• pp.51-57
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• 2000

Changes in the proline accumulation of ten-day-old seedlings of Vigna angularis in response to NaCl treatment were monitored. The proline content increased gradually both with an increase in the exposure time to salt stress and in a concentration-dependent manner. The increased proline accumulation was stronger in the shoots than in the roots. The salt stress by itself resulted in a significant inhibition of the chlorophyll content. Pre-treatment with proline before salinization lasting 48 h did not significantly affect the endogenous proline level in the roots, in contrast, a considerable increase of proline was observed in the shoots. The application of exogenous proline to the seedlings increased the endogenous proline content and improved the root and shoot growth under saline conditions. Detached leaves also exhibited an increased proline level in response to the applied NaCl, however, at a lower magnitude than in the intact seedlings. The proline alleviated the inhibitory effect of the NaCl in a concentration-dependent manner, thereby suggesting that salinity is a strong inducer of proline accumulation. In addition, abscisic acid eliminated the inhibitory effect of the salt salinity, thereby indicating a protective role on salinity stress and a regulatory role in proline synthesis. Accordingly, it would appear that proline may be involved in salt tolerance.