• Speech Sciences
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• v.10 no.2
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• pp.169-189
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• 2003

Trained singers, one group of professional voice users, have much more interest on the voice than common people, and on its management, too. They train for singing beautiful songs, and, at the same time, try for efficient voice production. The present study was performed with three tenors and three baritones, undergraduate students majored in classical singing, to investigate the degree of improvement of their voice production efficiency through vocal function exercise, by measuring the three dependent variables, maximum phonation time, speed quotient of glottal contact, and the number of semi tones. For the baseline establishment, dependent variables were measured 3$\sim$6 times for two weeks. Then, the subjects exercised vocal function exercise for seven weeks, and after the termination of training, evaluation was performed four times for two weeks, to find the maintenance of the training effect. Vocal function exercise is composed of four successive steps: warm-up, stretching exercise, contracting exercise, power exercise. As results, all of six subjects showed improvement in the aspect of maximum phonation time, speed quotient if glottal contact, and the number of semitones.

• Steel and Composite Structures
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• v.26 no.5
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• pp.533-547
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• 2018

In this paper the time-dependent creep analysis of a thick-walled FG cylinder with finite length subjected to axisymmetric mechanical and thermal loads are presented. First order shear deformation theory (FSDT) is used for description of displacement components. Inner and outer temperatures and outer pressure are considered as thermo-mechanical loadings. Both thermal and mechanical loadings are assumed variable along the axial direction using the sinusoidal distribution. To find temperature distribution, two dimensional heat transfer equation is solved using the required boundary conditions. The energy method and Euler equations are employed to reach final governing equations of the cylinder. After determination of elastic stresses and strains, the creep analysis can be performed based on the Yang method. The results of this research indicate that the boundaries have important effects on the responses of the cylinder. The effect of important parameters of this analysis such as variable loading, non-homogeneous index of functionally graded materials and time of creep is studied on the behaviors of the cylinder.

• Steel and Composite Structures
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• v.28 no.3
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• pp.331-347
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• 2018

In this paper history of stresses, strains, radial and circumferential displacements of a functionally graded thick-walled hollow cylinder due to creep phenomenon is investigated. The cylinder is subjected to an arbitrary non-axisymmetric two dimensional thermo-mechanical loading and uniform magnetic field along axial direction. Using equilibrium, strain-displacements and stress-strain relations, the governing differential equations of the problem containing creep strains are derived in terms of radial and circumferential displacements. Since the displacements are varying with time due to creep phenomenon, an analytical solution is not available for these equations. Thus, a semi-analytical procedure based on separation of variables and Fourier series together with a numerical procedure is employed. The numerical results indicate that the non-axisymmetric loading and the material grading index have significant effect on stress redistributions. Moreover, by proper selection of material for any combination of non-axisymmetric loading, one can arrive suitable response for the cylinder to achieve optimal design. With some simplifications, the results are validated with the existing literature.

• Journal of Energy Engineering
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• v.21 no.2
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• pp.138-143
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• 2012

The present study has been carried out to elucidate the unsteady temperature characteristics of a refrigerator near gasket region by measuring time dependent temperature measurements. From the measured values of the time dependent temperature inside and outside region of a refrigerator, one could see that the temperature varies periodically with time from the effect of refrigerator operation. The measured mean temperatures at the wall had great different value from the previous other research results conducted by numeric ofheat transfer using improperfheat transfer boundary condition. The present study could give the experiment ofdata for the properfnumeric ofheat transfer an oysis and suggest more accuratefheat transfer boundary conditions for the inside and outside of a refrigerator.

• Nuclear Engineering and Technology
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• v.30 no.6
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• pp.531-540
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• 1998

The DYLAM-3 code which overcomes the limitation of event tree/fault tree was applied to LOOP (Loss of Off-site Power) in the mid-loop operation employing HEPs (Human Error Probabilities) supplied by the ASEP (Accident Sequence Evaluation Program) and the SEPLOT (Systematic Evaluation Procedure for Low power/shutdown Operation Task) procedure in this study. Thus the time history of core uncovery frequency during the mid-loop operation was obtained. The sensitivity calculations in the operator's actions to prevent core uncovery under LOOP in the mid-loop operation were carried out. The analysis using the time dependent HEP was performed on the primary feed & bleed which has the most significant effect on core uncovery frequency. As the result, the increment of frequency is shown after 200 minutes duration of simulation conditions. This signifies the possibility of increment in risk after 200 minutes. The primary feed & bleed showed the greatest impact on core uncovery frequency and the recovery of the SCS (Shutdown Cooling System) showed the least impact. Therefore the efforts should be taken on the primary feed & bleed to reduce the core uncovery frequency in the mid-loop operation. And the capability of DYLAM-3 in applying to the time dependent concerns could be demonstrated.

• The Journal of Korean Society for Radiation Therapy
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• v.12 no.1
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• pp.166-173
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• 2000

Angiopoietin-1(Ang-1) is a vasculogenic factor that signals through the endothelial cell-specific Tie2 receptor tyrosine kinase. We examined the effect of angiopoietin-1(Ang-1) on radiation-induced apoptosis in human umbilical vein endothelial cells(HUVECS) and receptor/second messenger signal transduction pathway for Ang-1's effect on HUVECs. The percent of apoptotic cells under control condition(0Gy) was $8.2\%$. Irradiation induced apoptosis was increased in a dose(1, 5, 10, and 15Gy)- and time 12, 24, 48 and 72hr)-dependent manner. The percent of apoptotic cells was approximately $34.9\%$ after 15 Gy of irradiation. Under these conditions, pretreatment with Ang-1's (50, 100, 200, and 400 ng/ml) inhibited irradiation-induced apoptosis in human umbilical vein endothelial cells in a dose-dependent manner. Two hundred ng/ml of Ang-1 inhibited approximately $55-60\%$ of the apoptotic events that occurred in the 10 Gy-irradiated cells. Pre-treatment with soluble Tie2 receptor, but not Tie1 receptor, blocked the Ang-1's antiapoptotic effects. Phosphatidylinositol 3'-kinase (P13-kinase) specific inhibitor, wortmanin and LY294002, blocked the Ang-1-induced antiapoptotic effect. Ang-1 promotes the survival of endothelial cells in irradiation-induced apoptosis through Tie2 receptor binding and P13-kinase activation. Pretreatment of Ang-1 could be beneficial in maintaining normal endothelial cell integrity during irradiation therapy.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.1
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• pp.199-205
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• 2009

It is unclear whether Fructus ligustri Lucidi (FLL) extract anti-proliferative effect in human glioma cells. The present study was therefore undertaken to examine the effect of FLL on cell viability and to determine the underlying mechanism in A172 human glioma cells. Cell viability and cell death were estimated by MTT assay and trypan blue exclusion assay, respectively. Apoptosis was measured by Annexin-V binding assay and cell cycle analysis. Activation of kinases and caspase-3 was estimated by Western blot analysis. FLL resulted in apoptotic cell death in a dose- and time-dependent manner. FLL-induced cell death was not associated with reactive oxygen species generation. Western blot analysis showed that FLL treatment caused down-regulation of PI3K/Akt pathway, but not ERK. The PI3K/Akt inhibitor LY984002 sensitized the FLL-induced cell death and overexpression of Akt prevented the cell death. FLL induced caspase-3 activation and the FLL-induced cell death was prevented by caspase inhibitors. These findings indicate that FLL results in a caspase-dependent cell death through a P13K/Akt pathway in human glioma cells. These data suggest that FLL may serve as a potential therapeutic agent for malignant human gliomas.

• The Journal of Internal Korean Medicine
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• v.26 no.4
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• pp.853-863
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• 2005

Object : This study was performed to investigate the anti-fibrogenic effect of Artemisiae Capillaris Herba(ACH) on cultured rat hepatic stellate cells. Methods : Hepatic Stellate Cells were obtained from a 350gm Sprague-Dawley rat by tissue perfusion system, and the cells for the study were selected after 3 passages of culture on non-coated plastic culture dishes which enable the cells to activate, thus producing collagens in the cell media. Cells were treated with various concentrations of Artemisia Capillaris Herba(ACH) extract powder for 24 or 48 hours. After the treatment, Soluble collagen, procollagen levels and the mRNA of the procollagen type I C were measured by using assay kit and RT-PCR method. Results : Procollagen production by the hepatic stellate cells decreased after the treatment in a time-dependent dose-dependent manner. The mRNA expression decreased consistently with the volume of the secreted procollagen which indicates the herb hat inhibitory effect on fibrogenesis of the liver by regulating one of the fibrosis associated genes in transcription. Conclusion : These results suggest that ACH is beneficial in the treatment of cirrhotic patients as well as for the patients with chronic hepatitis.

• Biomedical Science Letters
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• v.17 no.3
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• pp.217-223
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• 2011

${\beta}$-sitosterol glucoside exists in a variety of plants and have anti-tumor, anti-microbial, and immunomodulatory activities. Mast cells and eosinophils play important roles in a variety of inflammatory diseases, specifically asthma and atopic dermatitis. In the present study, we used lactose-${\beta}$-sitosterol (L-BS) and investigated the effect of L-BS on inflammatory responses of the human mast cell line, HMC-1 and the human eosinophilic leukemia cell line, EoL-1. In HMC-1 cells, L-BS significantly inhibited cell migration in response to stem cell factor without cytotoxicity. However, the mRNA expression of CC chemokine receptors (CCRs), including CCR1-5, were not altered after L-BS treatment in HMC-1 cells. LPS-induced IL-4 production was also suppressed by L-BS in a dose-dependent manner. In EoL-1 cells, the concentration ranging from 0.1 ${\mu}M$ to 10 ${\mu}M$ of L-BS had no cytotoxicity and had no effect on mRNA expression of major protein-mediators derived from activated eosinophils. However, 100 ${\mu}M$ of L-BS induced the apoptosis of EoL-1 cells in a time-dependent manner. This finding indicates the possibility of L-BS as a potential therapeutic molecule in inflammatory diseases and may contribute to the need to improve current therapeutic drugs.

• Advances in Traditional Medicine
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• v.8 no.1
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• pp.24-31
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• 2008

In the present investigation, we studied the effect of alcoholic extract of Moringa oleifera (M. oleifera) seed kernels on various experimental models of bronchial asthma. Significant (P < 0. 05) increase in preconvulsion time was observed due to pretreatment with M. oleifera when the guinea pigs were exposed to either acetylcholine (Ach) or histamine aerosol. This bronchodilating effect of M. oleifera was comparable to ketotifen fumarate. Spasmolytic effect of M. oleifera was also observed by dose dependent inhibition of ideal contractions induced by Ach, 5HT, histamine and $BaCl_2$. Alcoholic extract of M. oleifera produced significant dose dependent protection by egg albumin and compound 48/80 induced mast cell degranulation. Pretreatment with alcoholic extract of M. oleifera also decreased carrageenan induced rat paw edema, which was comparable to that of standard diclofenac sodium. Minimum inhibitory concentration for alcoholic extract of M. oleifera was low as compared to cold-water extract and hot water extract when antimicrobial activity was tested against various respiratory pathogens like Escherichia coli (E. coli), Staphylococus aureus (S. aureus) and pseudomonas aeruginosa (P. aeruginosa). Our data suggest that antiasthmatic activity of M. oleifera seed kernels may be due to its bronchodilator, anti-inflammatory, mast cell stabilization and antimicrobial activity.